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Citations for Sartorius :
201 - 233 of 233 citations for Recombinant Human PPAR gamma Protein His tag since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2020Quote: ... The protein was loaded on a HiLoad 16/600 Superdex 200 pg column pre-equilibrated with storage buffer and the fractions containing pure Rel protein were collected and concentrated in a Vivaspin turbo 15 (Sartorius). The purity of protein preparations and the absence of RNA were assessed by SDS-PAGE and on a 1% agarose gel (in 1 × TAE buffer ...
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bioRxiv - Molecular Biology 2021Quote: ... the clarified supernatant was loaded onto Vivaspin protein concentrator columns with a molecular weight cut-off of 30 kDa (Sartorius) and centrifuged at 4,000 x g ...
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bioRxiv - Microbiology 2020Quote: ... Protein samples were quantified by BCA assay (Pierce) and transferred to a 10 kDa MWCO spin filter (Vivaspin 500; Sartorius) before centrifugation at 12,000 x g to collect denatured and reduced proteins atop the filter membrane ...
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bioRxiv - Immunology 2021Quote: ... Purified S proteins were exchanged to storage buffer and then filtered through 0.22 um filter (Sartorius Stedim Biotech, Göttingen, Germany). The concentration of purified protein was determined by ELISA ...
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bioRxiv - Molecular Biology 2021Quote: ... the clarified supernatant was loaded onto Vivaspin protein concentrator columns with a molecular weight cut-off of 30 kDa (Sartorius) and centrifuged at 4,000 x g ...
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bioRxiv - Biochemistry 2020Quote: ... The protein was expressed and purified as previously described.26 The purified protein was concentrated to 10-20 mg/mL using a Vivaspin15R (Sartorius), and purity was estimated to be at least 95% by SDS-PAGE.
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bioRxiv - Biochemistry 2021Quote: ... The protein solution was concentrated to 10 – 20 mg/mL using a Centrisart I MWCO 100 kDa concentrator (Sartorius AG).
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bioRxiv - Immunology 2021Quote: ... S2K146 and respective UCA Ab were prepared at 3 μg/ml and immobilized on pre-hydrated protein-A biosensors (Sartorius) for 75 sec ...
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bioRxiv - Immunology 2022Quote: KD measurements of the Pertuzumab and Trastuzumab IgMs to their Her2 antigen (Cat: 10004-HCCH, SinoBiological) were performed by first immobilizing them on Protein L (Cat: 18-5085, Sartorius) and CaptureSelect™ Biotin Anti-IgM Conjugate (Cat ...
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bioRxiv - Microbiology 2022Quote: ... The peak fractions corresponding to the protein complex were concentrated to about 16-20 mg/ml using a centrifugal filter with a PES membrane (Sartorius) and used for preparation of cryo-EM sample grids immediately.
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bioRxiv - Systems Biology 2022Quote: ... live-cell proliferation and expression of YFP-tagged proteins were tracked for 48 h using an Incucyte S3 device (Sartorius).
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bioRxiv - Cancer Biology 2023Quote: ... fractions containing the protein of interest were pooled together and concentrated using Vivaspin 20 centrifugal concentrators with a 5 kDa cut-off (Sartorius). The concentration of BRD4 BD1 was determined spectrophotometrically at 280 nm using the calculated molar extinction coefficient ...
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bioRxiv - Biophysics 2023Quote: ... The flow-through volume containing the untagged protein was concentrated to 10 mg/mL using Vivacon500 filters (50 kDa, Sartorius) for further purification using a Superdex 200 Increase 5/150 GL column equilibrated in 50 mM Tris-HCl pH 7.0 ...
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bioRxiv - Molecular Biology 2024Quote: ... The resulting clarified supernatant was loaded onto Vivaspin protein concentrator columns with a 30 kDa molecular weight cut-off (Sartorius) and centrifuged at 4,000 x g and 4 °C until the sample was concentrated by a factor of 100 ...
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bioRxiv - Plant Biology 2024Quote: ... Protein extracts were filtered on a glass fiber-pre-filter combined with a 0.45-µm syringe filter (Sartorius Technologies, Germany). Next ...
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bioRxiv - Biochemistry 2024Quote: ... and appropriate elution peak fractions from multiple runs were combined and concentrated using a protein concentrator (Sartorius, Vivaspin, 30,000 MWCO) to obtain a final sample of ∼300 μL at ∼30 µM concentration.
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bioRxiv - Immunology 2024Quote: HLA-A2+ tumor cell lines were lentivirally transduced to express either Nuclight-Red or Nuclight-Green nuclear-localizing fluorescent protein (Sartorius) and sorted for their expression ...
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bioRxiv - Immunology 2024Quote: ... EGFR CAR-T cells were placed at an E:T ratio of 1:1 with red fluorescent protein expressing (Nuclight Lenti-Red, Sartorius, USA) SKOV3 cells in the presence of absence of various doses of anti-VHH mAbs ...
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bioRxiv - Biochemistry 2020Quote: ... Protein containing fractions were pooled and concentrated to 5 mg/ml in a Vivaspin 500 (3 kDa) centrifugal concentrator (Sartorius AG), after which they were aliquoted ...
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bioRxiv - Biochemistry 2021Quote: ... The protein was concentrated to 25-30 µM with the use of a Centrisart I MWCO 100 kDa concentrator (Sartorius AG) and passed through a spin-out column of Bio-Gel P-6 equilibrated with the protein storage buffer (0.1 M Hepes-HCl ...
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bioRxiv - Cancer Biology 2024Quote: ... Glioma cells were stably transduced with a lentiviral nuclear red fluorescent protein under the EF1a promotor (Sartorius, cat. 4476, Göttingen, Germany) for visualization in live imaging assays ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... native Tau was purified from the cell extract by sequential anion exchange and phosphocellulose chromatography as previously described.69 The Tau protein eluate was dialyzed against water and concentrated with Vivaspin® (15R, 2,000 MWCO, Sartorius, UK). Protein concentrations were determined by a bicinchoninic acid (BCA ...
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bioRxiv - Biochemistry 2020Quote: ... bengalensis protein hydrolysates (BBPH) were subjected to centrifugal ultrafiltration using 3kDa cut-off membrane filtration unit (Vivaspin20, VS2091, Sartorius AG, Goettingen, Germany) to separate the low molecular peptide fraction (< 3kDa) ...
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bioRxiv - Microbiology 2022Quote: The affinity between each antibody and spike protein was measured by bio-layer interferometry (BLI) with a BLItz Biolayer Interferometer System (Sartorius, Goettingen, Germany).
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bioRxiv - Plant Biology 2023Quote: The biolayer interferometry was performed as previously described (Xie et al., 2022) using Octet® R2 Protein Analysis System (Sartorius, Figure 1) or Gator-plus (Gator Bio ...
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bioRxiv - Plant Biology 2023Quote: ... Finally, protein concentration and buffer exchange (20 mM Tris-HCl, pH 7.5) was performed using Vivaspin 30K columns (Sartorius, Germany, Göttingen, Germany).
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bioRxiv - Biophysics 2023Quote: ... proteins were exchanged to labelling buffer (20 mM HEPES, 50 mM NaCl, pH 7.5) using VivaSpin 6 concentrator (Sartorius AG, Gottingen, Germany).
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bioRxiv - Bioengineering 2022Quote: ... and HT29 cells were transfected to express the nuclear-restricted mKate2 red fluorescent protein using the IncuCyte® NucLight Lentivirus Reagents (Sartorius, Cat: 4476) and following the manufacturer’s protocol ...
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bioRxiv - Immunology 2021Quote: ... Protein production at pilot scale for nonclinical trial material was archived in 500L Bioreactor (BIOSTAT STR®500, Sartorius Stedim Biotech, Göttingen, Germany) using a fed-batch process using PowerCHO-2 medium as basal medium ...
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bioRxiv - Biochemistry 2020Quote: ... we concentrated the proteins from the supernate by membrane centrifugation using 5 K molecular weight cutoff Vivaspin units of 6 mL (Sartorius Stedim, Goettingen, Germany). The retentate (~300 μL ...
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bioRxiv - Biochemistry 2022Quote: ... The dialyzed 2C protein was centrifuged at 75,000 x g at 4 °C for 30 min to isolate soluble protein and concentrated using a Vivaspin Turbo 15 (Sartorius, 10 kDa MWCO) to 2 – 5 mL for preparative gel filtration ...
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bioRxiv - Microbiology 2023Quote: ... Fc-tagged RBD proteins (20 μg/mL) were captured on the protein A biosensor followed by 30 s of baseline run in 1x kinetics buffer (Sartorius Cat#: 18-1105). The biosensors were blocked with a control Fc protein (Jackson ImmunoResearch Cat# ...
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bioRxiv - Cancer Biology 2023Quote: ... of PD-L1 protein/PD-L1 antibody was determined by Octet Biolayer interferometry (BLI) using the Octet RED384 system (Sartorius, Bohemia, NY, USA). Briefly ...