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Citations for Sartorius :
51 - 100 of 233 citations for Recombinant Human PPAR gamma Protein His tag since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2023Quote: Human NIL iPSCs were cultured in Nutristem (Sartorius, #05-100-1A) supplemented with Pen/Strep 0.1% on geltrex-coated plates (Thermo Fisher Scientific ...
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bioRxiv - Immunology 2022Quote: ... Protein A biosensors (Sartorius, #18-5010) were hydrated in BLI assay buffer for 10-20 minutes and loaded onto an Octet Red96 BLI instrument (Pall ...
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bioRxiv - Pathology 2024Quote: Human bone specimens were homogenized by Mikro-Dismembrator U (Sartorius, Gottingen, Germany) and total RNA was isolated using the TRI Reagent (ThermoFisher Scientific ...
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bioRxiv - Neuroscience 2020Quote: ... using protein concentrator tubes (VIVASPIN 20, Sartorius). Both purified IgG fractions from patient and controls were re-tested on CBAs and the anti-IgLON5 IgG concentration was determined (titer 1:100) ...
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bioRxiv - Cancer Biology 2020Quote: Proteins were transferred to nitrocellulose membrane (Sartorius). The membranes were blocked with 10% BSA (Santa Cruz Biotechnology ...
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bioRxiv - Cell Biology 2024Quote: ... Proteins were concentrated using centrifugal concentrator (Sartorius) and further purified with size exclusion chromatography using a Superdex 200 column (GE Life Sciences) ...
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bioRxiv - Cancer Biology 2021Quote: ... Beads detecting cytokines from human were analyzed on an IQue Screener Plus (Sartorius). All assays were analyzed using quintuplicate determinations.
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bioRxiv - Evolutionary Biology 2022Quote: ... IgG was loaded onto Anti-Human Fc Capture (AHC) biosensors (Sartorius, #18-5060). To reduce the avidity effect ...
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bioRxiv - Immunology 2024Quote: Purified IgG1 mAbs were quantified using anti–human Fab-CH1 (FAB2G) biosensors (Sartorius) as previously described (58) ...
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bioRxiv - Microbiology 2023Quote: ... The eluted proteins were concentrated with a centrifugal device (MWCO 30 kDa for soluble EFNB2 proteins and 50 kDa for soluble Eph receptor proteins; Sartorius) before being separated on a Superdex 200 Increase 10/300 GL (Cytiva Life Sciences ...
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bioRxiv - Biochemistry 2020Quote: ... Proteins were concentrated using Vivaspin centrifugal filters (Sartorius) of appropriate cut-offs ...
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bioRxiv - Biochemistry 2020Quote: Purified proteins were concentrated with VIVASPIN concentrators (Sartorius) and dialyzed against E-Buffer with 1 mM DTT ...
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bioRxiv - Biochemistry 2022Quote: ... using a 100kDa MWCO Vivaspin protein concentrator (Sartorius), aliquoted ...
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bioRxiv - Biochemistry 2021Quote: ... Proteins were concentrated using Vivaspin concentrators (Sartorius Stedin) and the sample purity and homogeneity was assessed by SDS-PAGE.
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bioRxiv - Cell Biology 2024Quote: ... the protein was concentrated using Vivaspin column (Sartorius). Pellets from KCMF1-Strep expression were resuspended in 50mM Hepes/KOH pH7.5 ...
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bioRxiv - Molecular Biology 2022Quote: Human primary keratinocytes were plated in Essen ImageLock 96-well plates (Sartorius, Göttingen, Germany) at 15,000 cells per well for migration assay and in 12-well plates at 20,000 cells per well for cell growth assay ...
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bioRxiv - Biochemistry 2024Quote: The kinetics of the protein-protein interactions were determined using the bio-layer interferometry from the BLI system (Sartorius Octet BLI Discovery). Protein-protein interactions were observed by immobilizing 0.05 mg/ml of His-tagged VARP FL on a Ni-NTA biosensor or 0.05 mg/ml of biotinylated N-VARP on a streptavidin biosensor ...
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bioRxiv - Biophysics 2021Quote: ... Eluted protein was concentrated using centrifugal concentrators (VivaSpin, Sartorius) and then assessed for successful labeling by measuring fluorescence using the following settings and the monochromator in the BioTek Synergy Neo2 plate reader ...
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bioRxiv - Cell Biology 2023Quote: ... 2mM DTT and proteins concentrated via centrifugation (Vivaspin, Sartorius). Recombinant kinase was added to 10µl of 2X kinase buffer [40mM Hepes (pH 7.5) ...
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bioRxiv - Microbiology 2024Quote: Antibodies were loaded onto a protein A biosensor (Sartorius) for 300 s with a concentration of 10 μg/mL ...
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bioRxiv - Evolutionary Biology 2024Quote: ... Protein was concentrated (Vivaspin 20, 50 kDa MWCO, Sartorius), and flash frozen in liquid nitrogen until further use.
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bioRxiv - Biochemistry 2024Quote: ... The protein was concentrated in a Vivaspin 20 (Sartorius) concentrator (10-kDa MWCO ...
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bioRxiv - Cancer Biology 2023Quote: The human plasma samples were filtered (Minisart NML syringe filters, pore size: 0.8 µm; Sartorius) and diluted with 1x Phosphate-Buffered Saline without calcium and magnesium buffer (Gibco ...
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bioRxiv - Immunology 2023Quote: ... BLI binding experiments were performed with Anti Human IgG Fc (AHC) biosensors (Sartorius 18-5060). Using Octet buffer (PBS with 0.1% Tween20) ...
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bioRxiv - Biochemistry 2021Quote: ... All protein samples were concentrated using Vivaspin centrifugal concentrators (Sartorius). Denaturing gel electrophoresis was performed using NuPAGE 4-12 % Bis-Tris gels (Thermo Fisher Scientific) ...
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bioRxiv - Bioengineering 2024Quote: ... the refolded protein was concentrated using a VIVAFLOW50 system (Sartorius). The concentrated sample was filtrated with 0.22 µm membrane filter ...
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bioRxiv - Biophysics 2023Quote: ... Protein samples were concentrated by ultrafiltration (5 kDa Vivaspin, Sartorius) and further purified by reverse phase chromatography using a preparative C4 column (Vydac 214 TP ...
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bioRxiv - Biophysics 2023Quote: ... Protein samples were concentrated by ultrafiltration (5 kDa Vivaspin, Sartorius) and further purified by reverse phase chromatography using a preparative C4 column (Vydac 214 TP ...
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bioRxiv - Biochemistry 2023Quote: ... The cleaved protein was then concentrated (Vivaspin 30,000 MWCO, Sartorius) and ultracentrifuged in a TLA-120.2 rotor (Beckman Coulter ...
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bioRxiv - Cancer Biology 2022Quote: ... Then proteins were transferred into ultrafiltration tubes (10kDa, Sartorius AG) and washed three times with 50 mM NH4HCO3 at 14000g for 15 minutes ...
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bioRxiv - Biophysics 2023Quote: ... Purified proteins were concentrated using centrifugal filter devices (Vivaspin, Sartorius). Protein concentration was estimated from its absorbance at 280 nm ...
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bioRxiv - Microbiology 2024Quote: ... Protein sample was concentrated with a 10K MWCO concentrator (Sartorius) and further purified by size exclusion chromatography (Superdex 200 Increase 10/300 GL GE Life Sciences).
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bioRxiv - Immunology 2024Quote: ... Experiments were performed using Octet R2 Protein Analysis System (Sartorius) and affinity was calculated using Octet Analysis Studio Software ...
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bioRxiv - Biochemistry 2024Quote: ... using a 30kDa cut-off centrifugal protein concentrator (Vivaspin, Sartorius). The final protein concentration was estimated using a microplate spectrophotometer (Multiskan SkyHigh ...
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bioRxiv - Microbiology 2023Quote: We performed human plasma and avidity variant Abs’ binding using an Octet Red384 biolayer interferometer (Sartorius). We completed all the steps in 0.05% BSA and 0.002% Tween20 ...
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bioRxiv - Immunology 2024Quote: ... Monoclonal Moo1 and Moo2 antibodies were immobilized on anti-human IgG Fc Capture (AHC) biosensors (Sartorius) to capture monoclonal antibodies at a concentration of 50 µg/mL for 5 min and washed with octet buffer ...
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bioRxiv - Bioengineering 2024Quote: ... Biotinylated human serum albumin (25 μg/mL) was loaded for 600 sec on Octet SA Biosensors (Sartorius). Biosensor association to siRNA-L2 or siRNA-CQ-L2 duplexes diluted to the specified concentrations (1 to 0.00625 μM ...
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bioRxiv - Molecular Biology 2020Quote: ... Proteins were concentrated using 30 kDa cut-off Vivaspin columns (Sartorius) and their concentration was determined by their absorbance at 280 nm.
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bioRxiv - Biochemistry 2021Quote: ... Protein was concentrated to 32 mg/ml using centrifugal concentrators (Sartorius) with a relative molecular mass cut-off of 100K.
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bioRxiv - Synthetic Biology 2021Quote: ... Protein was concentrated using a Vivaspin 500 column (Sartorius, Goettingen, Germany) column following the manufacturer’s instructions.
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bioRxiv - Immunology 2023Quote: ... All proteins were diluted in Octet kinetics buffer (Sartorius, Göttingen, Germany). Antibodies were captured on anti-human Fc AHC2 tips (Sartorius) ...
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bioRxiv - Cancer Biology 2024Quote: ... concentrated 10x using Vivaspin™ protein concentrator spin columns (Sartorius, VS15T02) and stored at 4°C.
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bioRxiv - Microbiology 2023Quote: ... The purified proteins were concentrated in a Vivaspin turbo 15 (Sartorius) centrifugal filter device (cut-off 5 or 50 kDa) ...
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bioRxiv - Microbiology 2022Quote: ... Proteins were removed by ultrafiltration (3 kDa Vivaspin; Sartorius, Göttingen, Germany). ACE and ANSA hydrolysis activities were quantified in discontinuous HPLC-based assays at 30°C in lysis buffer ...
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bioRxiv - Molecular Biology 2023Quote: The MultiCyt® QBeads® PlexScreen Secreted Protein Assay Kit (Sartorius) was used to quantify the level of tumor necrosis factor alpha (TNF-a ...
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bioRxiv - Molecular Biology 2024Quote: ... The dialyzed protein was concentrated with Vivaspin Turbo 4 concentrator (Sartorius) with a 100 kDa MWCO.
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bioRxiv - Bioengineering 2023Quote: ... Antibody CSL362 hIgG1 (captured ligand) was captured by Anti-Human Fc capture biosensor (AHC) (Sartorius, PN: 18-5060) for 300s at 0.5µg/mL ...
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bioRxiv - Cell Biology 2024Quote: Human PSCs including iPSCs and ESCs were cultured in NutriStem® hPSC XF Medium (Sartorius, 05-100-1A) after coating with 50 μg/μL Vitronectin (VTN-N ...
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bioRxiv - Biochemistry 2020Quote: ... Protein was concentrated using a Vivaspin centrifugal concentrator (MWCO 10 kDa, Sartorius) following the manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: ... The protein was concentrated with Vivaspin 20 concentrators (30 kDa MWCO, Sartorius). Concentrated protein was further purified by size exclusion chromatography on a HiLoad 16/600 Superdex 200 pg column (Cytiva) ...