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Citations for Sartorius :
1 - 50 of 233 citations for Recombinant Human PPAR gamma Protein His tag since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2021Quote: ... recombinant SARS-CoV-2 S2-His-tag at 10 µg/mL was loaded on Anti-Penta-HIS (HIS1K) biosensors (Sartorius Corporation) for 1 min ...
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bioRxiv - Biochemistry 2022Quote: Anti-His-tag (HIS1K) biosensors (Sartorius) were incubated in BLI buffer (0.002% v/v Tween-20 in 1× PBS ...
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bioRxiv - Immunology 2021Quote: ... S2-His-tag was immobilized on Anti-Penta-His (HIS1K) biosensors (Sartorius Corporation), and a concentration series of S2 binding mAb Fab at 200 ...
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bioRxiv - Biophysics 2024Quote: ... His-tagged dROS1 protein (construct 2-3) were loaded onto penta-His sensors (Sartorius) to a response unit of about 0.5 nm followed by quenching in 10 ng/mL biocytin (Sigma-Aldrich ...
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bioRxiv - Immunology 2021Quote: RBD-His-tag at 5 µg/mL was first loaded on Ni-NTA (NTA) biosensors (Sartorius Corporation) for 15 sec and subsequently blocked with 5 µg/mL mAbs or BLI assay buffer for 5 min ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... The biotinylated SYK-tSH2 (50 μg/mL, C-terminal AviTag, N-terminal his tag cleaved) was loaded onto Octet Super Streptavidin Sensors (Sartorius 18-5057) in 50 μL 1XBLI kinetic buffer (Octet Kinetics Buffer 10 X ...
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bioRxiv - Systems Biology 2024Quote: ... The tag-cleaved protein was concentrated using a 100 kDa cutoff centrifugal concentrator (Sartorius) and subjected to size exclusion chromatography using a Superdex 200 10/300 GL column (GE Healthcare ...
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bioRxiv - Microbiology 2020Quote: ... For all recombinant proteins,protein-containing fractions were pooled and buffer exchange to PBS via VivaSpin columns (Sartorius) was performed ...
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bioRxiv - Synthetic Biology 2022Quote: ... His-tagged antigens using Anti-Penta-HIS biosensors (Sartorius/ForteBio). Antigen was loaded to a threshold of 1 nm shift ...
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bioRxiv - Microbiology 2023Quote: ... The tag-free RnpM protein was collected and concentrated using a Vivaspin turbo 15 (Sartorius, Germany).
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bioRxiv - Cell Biology 2024Quote: ... This recombinant protein was biotinylated for further assessment using the avidin-utilizing Octet R2 (Sartorius, Germany).
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bioRxiv - Immunology 2021Quote: ... Anti-penta-HIS biosensors (Sartorius) were immersed in kinetics buffer (PBS + 0.5% BSA + 0.05% Tween-20 ...
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bioRxiv - Immunology 2022Quote: ... Anti-penta-HIS biosensors (Sartorius) were immersed in kinetics buffer (PBS + 0.5% BSA + 0.05% Tween-20 ...
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bioRxiv - Biochemistry 2024Quote: ... The supernatant containing soluble His-SUMO-GLT25D1/COLGALT1 protein was filtered through a MiniSart GF 0.8 μm filter (Sartorius), loaded onto a 5 mL NiSepharose Excel column (Cytiva ...
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bioRxiv - Biochemistry 2024Quote: ... HNV-F proteins with the twin-strep tag at 6 µg/mL were captured onto Streptavidin (SA) Biosensors (Sartorius) for 300s ...
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bioRxiv - Biochemistry 2022Quote: ... or HIS 1K biosensors (Sartorius/ForteBio) (CrossMAb ...
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bioRxiv - Immunology 2024Quote: ... Anti-Penta-His (HIS1K) sensor tips (Sartorius) were pre-equilibrated in HEPES buffered saline (0.15M sodium chloride ...
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bioRxiv - Plant Biology 2024Quote: ... MBP-His-KD and MBP-His-PKD) were diluted to 100nM in Octet® Kinetics Buffer (SARTORIUS, #18-1105) for immobilized to Octet® Ni-NTA (NTA ...
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bioRxiv - Immunology 2021Quote: ... His-tagged ACE2 was immobilized onto Ni-NTA coated biosensors (Sartorius) before association of increasing concentrations of each RBD for 120 seconds followed by dissociation using HBS pH 7.4 for 360 seconds ...
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bioRxiv - Microbiology 2024Quote: ... sACE2-His (1 or 2 µg/ml)-coated HIS2 sensors (Sartorius) were incubated with various concentration of VHH-B07-Fc ...
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bioRxiv - Immunology 2024Quote: ... Octet Anti-Penta-His (HIS1K) sensor tips (Sartorius ForteBio 18-5120), Octet Streptavidin (SA ...
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bioRxiv - Bioengineering 2022Quote: ... and was captured by anti-Penta-His (HIS1K) sensors (Sartorius, 18-5120). The primary antibody was diluted to 150nM with a running buffer in wells ...
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bioRxiv - Microbiology 2022Quote: ... The aliquots containing the purified His-ChuP were concentrated using a VivaSpin 6 column (Sartorius), and desalted by gel filtration in PD-10 column (GE Healthcare ...
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bioRxiv - Immunology 2021Quote: His-tagged FcαRI (Sinobiological, Cat: 10414-H08H) were immobilized via Ni-NTA biosensor (Sartorius, Cat: 18-5101) and subject to free floating IgA variants to obtained ka ...
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bioRxiv - Cell Biology 2022Quote: ... His-PKR was further concentrated to at least 10 mg/ml using Vivaspin centrifugal concentrator 30 K (Sartorius), snap-frozen in liquid nitrogen and stored at −80 °C.
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bioRxiv - Immunology 2022Quote: KD measurements of Fc-tagged Human FcμR (Cat: 13556-H02H, SinoBiological) immobilised on Anti-Human IgG Fc (AHC) (Cat: 18-5060, Sartorius) biosensors were performed on the Octet Red96® system with the loading threshold set at 1.0 nm and utilizing the recombinant Pertuzumab and Trastuzumab IgM VH whole antibodies variants in five serial diluted concentrations (12.5 to 200nM ...
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bioRxiv - Immunology 2021Quote: ... His-tagged SARS-CoV-2 RBD was prepared at 8 μg/ml in Kinetics buffer (PBS 0.01% BSA) and loaded on pre-hydrated anti-penta-HIS biosensors (Sartorius) for 2.5 min ...
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bioRxiv - Biophysics 2024Quote: ... The 50 ml containing 6-His-AdhE was concentrated to a final volume of 10.5 ml using a Vivaspin 20 centrifugal concentrator (Sartorius) with a molecular weight cut-off of 30 kDa and then dialysed against buffer A ...
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bioRxiv - Microbiology 2024Quote: ... VHH-His (or sACE2-His) (5 µg/ml) diluted into Sartorius’s PBS working buffer were immobilized onto Ni-NTA biosensors (Sartorius), which were pre-equilibrated in the same buffer ...
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bioRxiv - Biophysics 2024Quote: ... The protein elutes were concentrated using a protein concentrator (Sartorius #VS15T01). For protein labeling ...
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bioRxiv - Molecular Biology 2023Quote: ... The Hs53BP1 fragment without its GST tag was recovered in the flow through and concentrated using Vivaspin concentrator (Sartorius).
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bioRxiv - Immunology 2022Quote: ... Anti-Human Fc sensor tips (ForteBio, now Sartorius) were loaded with 200 nM mAb at a threshold of 0.8nm and then submerged into wells containing 100 nM (protomer/monomer concentration ...
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bioRxiv - Biochemistry 2023Quote: ... Anti-Human Fc sensor tips (ForteBio, now Sartorius) were loaded with 200 nM mAb or FcACE2 to a threshold of 0.8 nm and then submerged into wells containing 100 nM (protomer/monomer concentration ...
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bioRxiv - Immunology 2021Quote: ... His-tagged SARS-CoV-2 RBD was prepared at 8 µg/ml and loaded on pre-hydrated anti-penta-HIS biosensors (Sartorius) for 2.5 min ...
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bioRxiv - Biochemistry 2022Quote: The specificity of the binding consisted in the immobilization of 2 μg/mL of purified VHHs on Anti-His coated sensors (His1K, Sartorius) via their His6 tag ...
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bioRxiv - Immunology 2022Quote: ... His-tagged SARS-CoV-2 RBD was prepared at 8 μg ml−1 and loaded on prehydrated anti-penta-HIS biosensors (Sartorius) for 3 min ...
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bioRxiv - Biophysics 2024Quote: ... The purified proteins were then concentrated using a protein concentrator (Sartorius #VS15T01) and verified by SDS-PAGE (Fig ...
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bioRxiv - Microbiology 2024Quote: ... 5 µg/mL anti-ALFA tag biotinylated nanobody in BLI buffer was immobilized on the surface of streptavidin-coated biosensors (Sartorius) to produce a total response of 1-2 nm ...
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bioRxiv - Microbiology 2024Quote: ... protein A biosensors (Sartorius) were first loaded with 10 μg/mL of mAb/bsAb in the running buffer until a binding threshold of 1 nm was reached ...
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bioRxiv - Biochemistry 2022Quote: ... IgGs produced from the scFvs from the above sort were assessed for their competition of binding with one another using anti-Penta HIS (His1K) biosensors (Sartorius/ForteBio). His1K tips were pre-quenched with buffer containing 10nM biotin ...
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bioRxiv - Molecular Biology 2024Quote: ... Anti-human IgG Fc capture (AHC) biosensor tips (Sartorius) were equilibrated for ten minutes in a 1:4 mixture of conditioned Freestyle 293 medium and 1X HBS EP+ to match the diluted media ...
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bioRxiv - Microbiology 2024Quote: ... the anti-human IgG Fc capture (AHC) biosensor (Sartorius) was conditioned in the detergent-containing Kinetic Buffer (KB ...
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bioRxiv - Microbiology 2024Quote: Protein–protein interactions were measured using the Octet N1 system (Sartorius, Göttingen, Germany). His-SeFtsZ and His-SeSepF were diluted in BLI buffer (20 mM Tris-HCl pH 7.4 ...
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bioRxiv - Biophysics 2024Quote: ... The Cy5-labeled tau proteins were then concentrated using a protein concentrator (Sartorius #VS15T01).
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bioRxiv - Immunology 2020Quote: ... Protein A biosensors (FortéBio/Sartorius) were first hydrated for 10 minutes in Kinetics buffer ...
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bioRxiv - Biochemistry 2023Quote: ... each Protein A biosensors (Sartorius) was pre-equilibrated in PBST buffer (PBS ...
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bioRxiv - Immunology 2023Quote: ... Antibodies were captured on anti-human Fc AHC2 tips (Sartorius). Data capture was performed using Octet Data Acquisition software version 9.0 (ForteBio) ...
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bioRxiv - Synthetic Biology 2024Quote: The binding properties of the Fab to PA tags were analyzed using biolayer interferometry (BLI) on the BLItz system (Sartorius Japan, Japan). The APS biosensor was hydrated in PBS for 10 minutes prior to use ...
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bioRxiv - Immunology 2023Quote: The binding affinity of recombinant antibodies to antigens was estimated by bio-layer interferometry (Octet® QK, ForteBio/Sartorius). Antibodies were loaded at 10-20 nM onto anti-human IgG Fc capture or anti-Mouse IgG Fc capture biosensors ...
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bioRxiv - Cancer Biology 2023Quote: ... Its binding to LIF was confirmed using recombinant LIF (ACRO Biosystems, LIF-H52H3) on an Octet RED 96e biolayer interferometry instrument (Sartorius).