Labshake search
Citations for Sartorius :
151 - 200 of 3095 citations since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Immunology 2024Quote: Fab binding kinetics were measured using biolayer interferometry on an Octet 96 Red instrument (ForteBio) using FAB2G biosensors (Sartorius). C7 and C74 Fabs were diluted to 10 μg/mL in Kinetics Buffer (KB ...
-
bioRxiv - Microbiology 2024Quote: ... 50 mL of the supernatant was further filtered through sterile syringe filters (0.2 μm Sartorius) and then concentrated using a speedvac concentrator to a final volume of 500 μL ...
-
bioRxiv - Microbiology 2024Quote: ... Fecal virome isolation and purification were carried out according to our previous method with the following modifications 16: the Centriprep 50K was replaced by Centrisart® I centrifugal ultrafiltration unit (MWCO 100 kDa, Sartorius Stedim Biotech GmbH) and the enrichment step was conducted at 2500 × g for 30 min at 4°C ...
-
bioRxiv - Immunology 2024Quote: ... Antibodies were diluted to a final concentration of 20 ug/mL before being immobilized on ProteinA tips (Sartorius) for 600 s ...
-
bioRxiv - Immunology 2024Quote: ... Plates were left at room temperature for 20 min and transferred to the Incucyte SX1 instrument (Sartorius) for baseline images ...
-
bioRxiv - Microbiology 2024Quote: ... cells were imaged in the Incucyte SX5 Live-Cell Analysis System (Sartorius). Cell confluence and the percentage of GFP-positive area were quantified automatically with the Incucyte Analysis software ...
-
bioRxiv - Microbiology 2024Quote: ... The IncuCyte™ S3 HD live-cell imaging platform (Sartorius) is a wide-filed microscope mounted inside a tissue culture incubator and is controlled by IncuCyte™ software ...
-
bioRxiv - Microbiology 2024Quote: The Octet Red96 BLI platform (Sartorius BioAnalytical Instruments ...
-
bioRxiv - Cancer Biology 2024Quote: ... Caspase-3/7 Green Reagent for Apoptosis (Sartorius; cat. 4440) was added to the cell culture media ...
-
bioRxiv - Cancer Biology 2024Quote: ... and filter-sterilized using 0.22 µm Minisart® PES syringe filters (Sartorius, 16532-K). The filtered conditioned media from the transduced PC-3 were diluted 1:1 with fresh serum-free RPMI 1640 base medium ...
-
bioRxiv - Microbiology 2024Quote: ... HPLC (High Performance Liquid Chromatography) amino acid analysis of the supernatants was carried out by Sartorius Xell GmbH (Göttingen ...
-
bioRxiv - Neuroscience 2024Quote: ... the purified AAVs contained in the 40% iodixanol fraction were aspirated and transferred to 10,000 molecular weight cutoff Vivaspin tubes (Sartorius, Göttingen, Germany) for dialysis in PBS ...
-
bioRxiv - Microbiology 2024Quote: ... and buffer exchange to PBS using VivaSpin columns (Sartorius) was carried out ...
-
bioRxiv - Microbiology 2024Quote: ... 10 % glycerol) and proteins were concentrated using vivaspin 6 columns with a 5000 Da cut off (Sartorius). To remove the His-tag ...
-
bioRxiv - Microbiology 2024Quote: ... pooled enzyme samples after affinity chromatography were concentrated using centrifugal concentrators (10 kDa cutoff) (Sartorius, Germany) and applied to size exclusion chromatography (Superdex 200 HiLoad 26/60 prep grade column or Superose 6/100 column (Cytavia ...
-
bioRxiv - Microbiology 2024Quote: ... 500 µl glass beads (diameter 0.10–0.11 mm, Sartorius AG, Göttingen, Germany) were added to 1 mL sample and cells disrupted with a FastPrep®24 homogenizer (M ...
-
bioRxiv - Microbiology 2024Quote: Infected MRC-5 or ARPE19 cells were place into Incucyte S3 (Sartorius) at 2 hpi ...
-
bioRxiv - Microbiology 2024Quote: ... G3PDH containing fractions as judged by SDS-PAGE and activity measurements were pooled and imidazole was removed using a centrifugal concentrator (10 kDa cutoff, Sartorius, Germany). Protein aliquots were frozen in liquid nitrogen and stored at −80 °C for further use ...
-
bioRxiv - Immunology 2024Quote: BLI experiments were carried out using an Octet RED96e Biolayer Interferometer (Sartorius AG, Gottingen, Germany) using the Data Acquisition 12.0 software ...
-
bioRxiv - Immunology 2024Quote: ... Biotinylated OspA (5 µg/mL) in PBS containing 2% w/v BSA was captured onto streptavidin biosensors (#18-5019, Sartorius) for 5 min ...
-
bioRxiv - Immunology 2024Quote: ... Target-specific cytotoxic activity was assessed by imaging co-cultures using an Incucyte Sx5 (Sartorius) instrument and calculating the survival or outgrowth of fluorescent target cells over time.
-
bioRxiv - Immunology 2024Quote: ... Biotinylated mAbs were immobilized on streptavidin-coated biosensors (Sartorius, Goettingen, Germany) for 60 seconds at 50 µg/ml concentration ...
-
bioRxiv - Immunology 2024Quote: ... containing 0.1% BSA using Octet Red 96 (Sartorius, Goettingen, Germany). Biotinylated mAbs were immobilized on streptavidin-coated biosensors (Sartorius ...
-
bioRxiv - Immunology 2024Quote: ... The antibodies were buffer-exchanged into PBS and concentrated using 10,000 MWCO Vivaspin centrifugal spin columns (Sartorius).
-
bioRxiv - Immunology 2024Quote: ... 3C protease was removed using Ni-NTA resin and RBD-SD1 was buffer-exchanged into PBS and concentrated using 10,000 MWCO Vivaspin centrifugal spin columns (Sartorius).
-
bioRxiv - Immunology 2024Quote: ... Incucyte® Caspase-3/7 Dye (Sartorius, 4440). Antibodies ...
-
bioRxiv - Immunology 2024Quote: For quantification of cell dead events using IncuCyte (Sartorius), THP-1 cells were plated on a 24 or 96 well plate at a density of 4×108/well or 1×108/well ...
-
bioRxiv - Immunology 2024Quote: ... 10% FBS was added to the cells and real-time cell death analysis was performed using IncuCyte S3 Live-Cell Analysis instrument (Sartorius).
-
bioRxiv - Immunology 2024Quote: Real-time cell-death assays were performed using a two-color IncuCyte S3 Live-Cell Analysis instrument (Sartorius). Cell lines were seeded in 12 well plates and treated with cell death stimulating agents or infected with viruses ...
-
bioRxiv - Immunology 2024Quote: ... 2.5 µM Caspase-3/7 Green Dye (Sartorius, 4440) was used ...
-
bioRxiv - Biochemistry 2024Quote: ... Octet Streptavidin Biosensors (Sartorius, #18-5019) were used for all experiments ...
-
bioRxiv - Immunology 2024Quote: ... the stained cells were visualized using Incucyte S3 (Sartorius; GUI software versions 2019B Rev1 and 2021B).
-
bioRxiv - Microbiology 2024Quote: The sterile filtered fecal supernatant was concentrated using Centrisart centrifugal filters with a filter cut-off at 100 kDA (Sartorius) by centrifugation at 1,500 x g at 4 °C (dx.doi.org/10.17504/protocols.io.b2qaqdse) ...
-
bioRxiv - Microbiology 2024Quote: ... The fecal supernatant was filtered through a 0.45 µm Minisart High Flow PES syringe filter (Sartorius) to remove bacteria and other larger particles ...
-
bioRxiv - Evolutionary Biology 2024Quote: ... approximately 4 mg of pulverized tissue was weighed using an ultra-micro balance (CP2P Microbalance, Sartorius GmbH, Goettingen, Germany), packaged into tin capsules ...
-
bioRxiv - Cancer Biology 2024Quote: ... The area covered by migrating cells was measured with the Incucyte analysis program (Sartorius).
-
bioRxiv - Cancer Biology 2024Quote: ... Images were taken every 15 min for 48 hrs on an Incucyte S3 Live-cell analysis system (Sartorius, 4X/0.2). The area covered by migrating cells was measured with the Incucyte analysis program (Sartorius).
-
bioRxiv - Immunology 2024Quote: ... GFP expression was measured by using Incucyte S3 (Sartorius, Goettingen, Germany).
-
bioRxiv - Microbiology 2024Quote: ... the streptavidin sensor (Sartorius Corp) was coated with 50 nM Biotin-RBD or Biotin-HEL in 1×PBS for 30 min at 20°C ...
-
bioRxiv - Microbiology 2024Quote: ... The supernatants containing the fibrils were recovered and concentrated on vivaspin® 3 KDa (Sartorius, VS04T91) at 3,000 g ...
-
bioRxiv - Immunology 2024Quote: The binding kinetics of ZBP1-Zα1Zα2 with SARS-CoV-2 RNAs were evaluated using the Octet RED96 instrument (Sartorius). First ...
-
bioRxiv - Immunology 2024Quote: ... Binding was read by flow cytometry on an IntelliCyte High Throughput Flow Cytometry (IntelliCyte/Sartorius).
-
bioRxiv - Immunology 2024Quote: ... Supernatants were quantitated for antibody concentrations by bio-layer interferometry (BLI) on an Octet Red 96 (Sartorius) using protein A biosensors and a human IgG1 isotype control antibody as a standard ...
-
bioRxiv - Biophysics 2024Quote: ... Fractions with nanodiscs were concentrated using VivaSpin® 500 MWCO 100 kDa concentrators (Sartorius) to the target concentration (around 2 mg/mL).
-
bioRxiv - Cancer Biology 2024Quote: ... Initial data processing was performed using the ForeCyt 8 software (Sartorius). The AUC of viability and differentiation were calculated with in-house R scripts ...
-
bioRxiv - Cancer Biology 2024Quote: ... Cell numbers were counted before treatment and 24h after treatment using Incucyte® Live-Cell Analysis System (Sartorius) that automatically counts cells based on dedicated analysis adapted on specific cell lines morphology ...
-
bioRxiv - Cancer Biology 2024Quote: ... Cell growth was monitored for 48h after treatment using Incucyte® Live-Cell Analysis System (Sartorius) equipped with a FITC filter allowing the detection of cleaved caspase 3/7 leading to the cleavage of DEVD peptide and the release of green fluorescence ...
-
bioRxiv - Evolutionary Biology 2024Quote: ... We obtained the GFP Integrated Intensity (GCU·µm²/image) in each condition using a live-cell imaging platform (Incucyte SX5, Sartorius) at 24 hours post mixing and standardized to the signal obtained from the positive control (Wuhan-Hu-1 spike protein) ...
-
bioRxiv - Cancer Biology 2024Quote: ... and 1µg/µl of CpG and 0.9ul of caspase-3/7 dye (Sartorius, 4440) were added to the well for 72hrs incubation in the SpectraMax M5 and Lmax microplate reader ...
-
bioRxiv - Cell Biology 2024Quote: ... pollen contained in 5 ml suspension was vacuum-filtrated onto a round polyamide membrane filter (diameter 5 cm, pore size 0.45 µm; Sartorius) and transferred onto 3.5 ml solid (0.25 % phytagel [Sigma-Aldrich] ...