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Citations for Sartorius :
301 - 350 of 3095 citations since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2024Quote: Biolayer interferometry (BLI) experiments were performed at room temperature using Octet Red96e instrument (Sartorius). Biotin-labeled stem peptides (GenScript ...
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bioRxiv - Biochemistry 2024Quote: All BLI experiments were conducted on an Octet Red96 instrument (Forte Bio) using streptavidin biosensors (Sartorius 18-5019). Streptavidin biosensors were preincubated with BLI buffer (20mM Tris ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: Bio-Layer Interferometry (BLI) was performed using the Octet RED384 system (Sartorius BioAnalytical Instruments). The biotinylated SYK-tSH2 (50 μg/mL ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... The biotinylated SYK-tSH2 (50 μg/mL, C-terminal AviTag, N-terminal his tag cleaved) was loaded onto Octet Super Streptavidin Sensors (Sartorius 18-5057) in 50 μL 1XBLI kinetic buffer (Octet Kinetics Buffer 10 X ...
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bioRxiv - Biochemistry 2024Quote: ... phages were filtered through a 0.45-µm syringe filter (Sartorius). Full-length human and mouse CNPase were conjugated to desthiobiotin-N-hydroxysuccinimide ester (Berry and Associates) ...
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bioRxiv - Biochemistry 2024Quote: ... The complex was concentrated to 4.8 mg ml-1 using a 50,000 molecular weight cut-off concentrator (Sartorius Vivaspin20). Crystallization conditions were screened manually using the hanging-drop vapor-diffusion method in 48-well plates (Hampton VDX greased ...
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bioRxiv - Biochemistry 2024Quote: ... 4°C using a 3 kDa MWCO spin concentrator (Sartorius) until the final volume was 0.5 mL ...
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bioRxiv - Biochemistry 2024Quote: ... Finally pure FGF1 was concentrated to 150 µM using a 3 kDa MWCO 500 µL spin concentrator (Sartorius) then snap frozen in liquid nitrogen and stored at −80°C in 200 µL fractions ...
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bioRxiv - Biochemistry 2024Quote: ... Sensorgrams were fitted using the kinetic model with a mass transport parameter in QDat software (Sartorius).
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bioRxiv - Biochemistry 2024Quote: Purified PK-His6-Pol IVLF and its variants were immobilized onto Octet NTA biosensors (Sartorius, 18-5103) and incubated with varying concentrations of β2 clamp (31.3 to 8,000 nM as a dimer) ...
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bioRxiv - Biochemistry 2024Quote: SPR experiments were performed on a SensiQ Pioneer FE (Sartorius) at 25°C ...
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bioRxiv - Biochemistry 2024Quote: ... Shifts in the interference pattern upon interaction between Pol IVLF and β2 clamp were measured in real-time using Octet RED384 (Sartorius). To determine equilibrium dissociation constants ...
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bioRxiv - Biophysics 2024Quote: ... or tilted bottoms (Sartorius). Biosensors were hydrated in Sartorius Kinetics Buffer for 5 minutes prior to use ...
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bioRxiv - Biophysics 2024Quote: ... Binding curves were processed and fit using Octet Analysis Studio Software (Sartorius).
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bioRxiv - Biophysics 2024Quote: All measurements were performed on a FortéBio Octet RED384 instrument using Octet Ni-NTA biosensors (Sartorius) and 384-well black microplates with either flat (Greiner ...
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bioRxiv - Biochemistry 2024Quote: ... Biotinylated minibinders were diluted to a final concentration of 0.01 mg/mL in kinetics buffer and loaded onto streptavidin biosensors (Sartorius). TLR3 was diluted in kinetics buffer and its association was measured for 300 s ...
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bioRxiv - Biochemistry 2024Quote: ... Human TLR3-FC (Acro Biosystems) was diluted to 0.01 mg/mL in kinetics buffer and loaded onto Protein A tips (Sartorius). The cell assay constructs were diluted to 10 μM in kinetics buffer and their association was measured for 300 s ...
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bioRxiv - Physiology 2024Quote: ... bees were then placed in a refrigerator so that they would slow down enough to allow a body mass measurement to be taken on a microbalance (Sartorius Entris II, BCA224I-1S). Bees were released back at their nest boxes once all bees were tested for the day so that the same bee was not tested twice throughout the day ...
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bioRxiv - Cancer Biology 2024Quote: ... a wound was created in the cell monolayer using the WoundMaker (Sartorius, 4563) as per the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2024Quote: Invasion capacity was evaluated using a 96-well ImageLock plate (Sartorius, 4379) coated with Matrigel (Sigma-Aldrich ...
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bioRxiv - Cancer Biology 2024Quote: ... Cell images were acquired at various time intervals using the IncuCyte ZOOM Imaging System (Sartorius), and cell adhesion was determined through cell shape analysis and scoring.
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bioRxiv - Cancer Biology 2024Quote: ... 96-well ImageLock plates (Sartorius, 4379) were coated with Matrigel (Sigma-Aldrich ...
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bioRxiv - Cancer Biology 2024Quote: To assess cellular proliferation the Incucyte S3 Live-Cell Analysis System (Sartorius, Göttingen, Germany) was used to obtain growth curves with a high sensitivity ...
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bioRxiv - Biophysics 2024Quote: BLI experiments were performed at room temperature on a BLItz instrument (ForteBio) using Octet® Streptavidin Biosensor tips (Sartorius) and 4 µL sample drop holder ...
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bioRxiv - Cancer Biology 2024Quote: ... 3 x 104 WM852 or 5 x 104 WM793 cells were seeded in a 96-well ImageLock plate (Sartorius, 4379) and allowed to form a monolayer for 24 h prior to the assay ...
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bioRxiv - Microbiology 2024Quote: ... M9 was then filtered through a 0.22 μm Minisart® Syringe Filter (Sartorius, Germany). Tachyplesin-1 and tachyplesin-1-NBD were synthesised by WuXi AppTech (Shanghai ...
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bioRxiv - Microbiology 2024Quote: ... Multivariate statistical analyses were performed on these features using SIMCA® software 15.0 (Sartorius, Germany). First ...
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bioRxiv - Microbiology 2024Quote: ... These enrichment cultures were passed through a 0.22 µm filter to remove the remaining cells (Minisart High Flow PES, Sartorius), and the filtrate was used with the superhost in an agar overlay for viral plaque isolation as described above ...
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bioRxiv - Microbiology 2024Quote: ... 100 kDa MWCO vivaspin concentrator units (Sartorius VS04T42) equilibrated with VSB were used for washing and purifying the collected virus band ...
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bioRxiv - Molecular Biology 2024Quote: ... The resulting clarified supernatant was loaded onto Vivaspin protein concentrator columns with a 30 kDa molecular weight cut-off (Sartorius) and centrifuged at 4,000 x g and 4 °C until the sample was concentrated by a factor of 100 ...
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bioRxiv - Microbiology 2024Quote: ... 4G2 for 30 minutes at 4°C and washed in cytoperm/wash buffer twice before quantification of APC+ cells by flow cytometry (Intellicyt iQue Screener PLUS, Sartorius AG). Antibody 4G2 was isolated and purified by the Fred Hutchinson Cancer Center Antibody Technology core following expansion of the hybridoma D1-4G2-4-15 (Cat# HB-112 ...
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bioRxiv - Microbiology 2024Quote: ... Supernatants were transferred to new tubes and filtered using Minisart cellulose acetate syringe filters with a 1.2 µm pore size (Sartorius). The filtrate was transferred to ultra-clean 2 mL tubes and 280 µL were collected for nucleic acid extraction using the QIAamp Viral RNA mini kit (Qiagen) ...
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bioRxiv - Microbiology 2024Quote: ... Cells infected with DENV2-GFP were fixed with 2% paraformaldehyde and GFP+ cells quantified by flow cytometry (Intellicyt iQue Screener PLUS, Sartorius AG).
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bioRxiv - Microbiology 2024Quote: ... Supernatants were then filtered using Minisart cellulose acetate syringe filters with a 1.2 µm pore size (Sartorius) and transferred to ultra-clean 2 mL tubes ...
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bioRxiv - Microbiology 2024Quote: ... Supernatants were filtered (0.2 µm filter, Sartorius), treated or not for one hour at 56°C with 0.4 mg/mL proteinase K (P8107S ...
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bioRxiv - Microbiology 2024Quote: ... cells were processed according to the protocol described above (section “Viruses”) and infection was quantified by flow cytometry (Intellicyt iQue Screener PLUS, Sartorius AG).
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bioRxiv - Biochemistry 2024Quote: The BLI measurements were conducted using a Fortebio (Sartorius) K2 Octet using fiber optic biosensors ...
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bioRxiv - Microbiology 2024Quote: ... a filtration cassette (Vivaflow 50R, 100,000 MWCO, Hydrostat, model VF05H4, Sartorius or Vivaflow 200 100,00 MWCO ...
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bioRxiv - Developmental Biology 2024Quote: ... The bead solution was filtered by a 0.22 µm syringe filter (Sartorius) into 500 µL of 500 mM MES buffer (pH 6.0) ...
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bioRxiv - Neuroscience 2024Quote: ... sterilized by filtration using a 0.22μm filter (Sartorius, Göttingen, Germany) and stored at –20°C) ...
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bioRxiv - Molecular Biology 2024Quote: ... Sample was applied to a Vivacon 30k MWCO spin filter (Sartorius, VN01H21) and centrifuged at 12,500 g for 15 min ...
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bioRxiv - Biochemistry 2024Quote: ... The resulting sample was subject to buffer exchange in 30 kDa MWCO Vivaspin Turbo centrifugal filters (Sartorius) against 25 mM HEPES/NaOH ...
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bioRxiv - Immunology 2024Quote: ... biotinylated antigens were loaded onto Streptavidin Biosensors (Sartorius), and His-tagged proteins were loaded onto NTA Biosensors (FortéBio) ...
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bioRxiv - Biochemistry 2024Quote: ... 30000 MWCO ultrafiltration centrifugal concentrator (Sartorius, #VS2022). The concentrated protein samples were then purified by size exclusion chromatography using a HiLoadTM 16/600 SuperdexTM 200 pg Gel Filtration Column (Cytivia ...
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bioRxiv - Immunology 2024Quote: ... biotinylated natural Ara h 2 (InBio) diluted in kinetics buffer (Dulbecco’s PBS, 1% BSA, 0.02% Tween-20) was loaded onto streptavidin sensors (Sartorius) for 100 seconds ...
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bioRxiv - Immunology 2024Quote: ... Experiments were performed using Octet R2 Protein Analysis System (Sartorius) and affinity was calculated using Octet Analysis Studio Software ...
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bioRxiv - Immunology 2024Quote: ... Streptavidin sensors (Sartorius) were loaded with biotinylated natural Ara h 2 (InBio) ...
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bioRxiv - Biochemistry 2024Quote: ... The supernatant containing soluble His-SUMO-GLT25D1/COLGALT1 protein was filtered through a MiniSart GF 0.8 μm filter (Sartorius), loaded onto a 5 mL NiSepharose Excel column (Cytiva ...
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bioRxiv - Biochemistry 2024Quote: ... The sample was concentrated using 30 kDa MWCO Vivaspin Turbo centrifugal filters (Sartorius) and subject to final polishing into a Superdex 200 Increase 10/300 GL (Cytiva) ...
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bioRxiv - Immunology 2024Quote: ... Fluorescent images of live cells were obtained hourly for 7 days using an IncuCyte SX3 live cell imaging and analysis system (Sartorius Göttingen, Germany), as previously described 15.