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Citations for Sartorius :
1 - 50 of 3095 citations since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2024Quote: ... Anti-FLAG M2 was immobilized on protein G biosensors (Sartorius Cat. No. 18-5082) at 5 µg/mL (in binding buffer ...
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bioRxiv - Cancer Biology 2024Quote: Internalization was assessed using the Incucyte Fabfluor-pH Antibody labeling reagent (Sartorius, Germany), following the manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2024Quote: ... the media was changed to 90 µL of growth media containing 1:1000 Incucyte Nuclight Rapid Red Dye (Sartorius, 4717) and 1:1000 NucView 488 Caspase-3/7 substrate (Biotium ...
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bioRxiv - Molecular Biology 2024Quote: ... Anti-human IgG Fc capture (AHC) biosensor tips (Sartorius) were equilibrated for ten minutes in a 1:4 mixture of conditioned Freestyle 293 medium and 1X HBS EP+ to match the diluted media ...
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bioRxiv - Microbiology 2024Quote: The MV-containing cell-free supernatant was concentrated using Vivaspin Ultra filtration unit (Sartorius, Göttingen, Germany) with a 100 kDa MwCO until the desired volume of supernatant was achieved ...
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bioRxiv - Molecular Biology 2024Quote: ... Fractions containing IMP2 were collected and concentrated via centrifugal filtration using Vivaspin columns (30,000 MWCO, Sartorius), resuspended in SP buffer (HEPES ...
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bioRxiv - Molecular Biology 2024Quote: ... The eluted IMP2 fractions were concentrated via centrifugal filtration using Vivaspin columns (30,000 MWCO, Sartorius). Protein concentrations were measured by both UV spectroscopy (ε = 280 nm ...
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bioRxiv - Cancer Biology 2024Quote: ... and plates were placed into an Incucyte Live-Cell Assay incubator (Sartorius) for 72 hours ...
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bioRxiv - Biochemistry 2024Quote: ... The 35-mer template with a biotin-TEG at the 5’-overhang was annealed to an 11-mer primer (Supplementary Table 1) and immobilized on a streptavidin-coated biosensor (SAX, Sartorius AG). A chimeric RNA-DNA primer demonstrating the highest affinity to Polα (45 ...
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bioRxiv - Cell Biology 2024Quote: ... the ImageLock plate was placed into the WoundMaker (Sartorius) to create a uniform scratch in each well ...
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bioRxiv - Cell Biology 2024Quote: ... Cells were seeded into a 96-well ImageLock plate (Sartorius) at 3×104 cells/well and cultured overnight ...
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bioRxiv - Cell Biology 2024Quote: The migratory ability of trophoblasts was measured using an IncuCyte® (Sartorius, Bohemia, NY, USA) scratch wound healing assay ...
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bioRxiv - Biochemistry 2024Quote: ... and concentrated over a 3 kDa MWCO membrane using a Vivaspin 15 centrifugal device (Sartorius). The final Ws cytochrome c552 fraction was buffer exchanged to 5 mM Na-phosphate pH 7.0 using a desalting PD-10 column (Cytiva) ...
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bioRxiv - Biochemistry 2024Quote: Samples were concentrated by Vivaspin centrifugal devices (MWCO 50 kDa; Sartorius) before deposition on grids for cryogenic electron microscopy (Cryo-EM).
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bioRxiv - Biochemistry 2024Quote: ... concentrated using a Vivaspin Turbo Ultrafiltration unit (10 kDa MWCO; Sartorius) and buffer-exchanged in 10 mM HEPES ...
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bioRxiv - Biochemistry 2024Quote: ... The mixture was then concentrated using a Vivaspin Turbo Ultrafiltration unit (5 kDa MWCO; Sartorius) and buffer-exchanged to 20 mM tris-HCl ...
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bioRxiv - Biochemistry 2024Quote: ... concentrated using a Vivaspin Turbo Ultrafiltration unit (5 kDa MWCO; Sartorius) and buffer-exchanged to buffer L_A by a HiPrep 26/10 desalting column (Cytiva ...
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bioRxiv - Biochemistry 2024Quote: ... the monomer band harvested from a discontinuous sucrose gradient of the ΔsqdB strain was concentrated to 500 µL in a 50,000 MWCO centrifugal concentrator (Sartorius). This sample was split and incubated with and without 0.05% (w/v ...
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bioRxiv - Biochemistry 2024Quote: ... RC-LH1 monomer and dimer bands were buffer exchanged into 50 mM Tris at pH 7.5 with 100 mM NaCl and 0.03% w/v β-DDM by spin concentration with 50,000 MWCO centrifugal concentrators (Sartorius).
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bioRxiv - Biochemistry 2024Quote: ... The supernatant from this second spin step was subsequently passed through 2 0.22µm filters (Sartorius) and made up to 500 ml with 50 mM ammonium acetate buffer at pH 5 before loading onto a 30 mL SP Sepharose column (Cytiva) ...
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bioRxiv - Biochemistry 2024Quote: ... The mass of each “Ceres” sample was measured using an analytical balance (Sartorius, Krugersdorp, South Africa) and converted back to volume by assuming a density of 1 g/mL ...
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bioRxiv - Biochemistry 2024Quote: Binding kinetics of ZHER2-CK2α were measured by biolayer interferometry (BLI) on an OctetRed384 using in-house made HER2-Fc-AviTag immobilized on streptavidin biosensors (Sartorius, 18-5021). Kinetic measurements were performed in PBS with 0.1% bovine serum albumin and 0.05% Tween 20.
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bioRxiv - Biochemistry 2024Quote: ... and 1% Penicillin Streptomycin Solution (Biological Industries – Sartorius, 03-031-1B) under standard conditions (37 °C and 5% CO2 in a humidified incubator) ...
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bioRxiv - Biochemistry 2024Quote: ... 1% l-glutamine (Biological Industries – Sartorius, 03-020-1B), and 1% Penicillin Streptomycin Solution (Biological Industries – Sartorius ...
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bioRxiv - Biochemistry 2024Quote: ... and the peak corresponding to pure monomeric hP0 was collected and concentrated using Vivaspin centrifugal concentrators (Sartorius).
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bioRxiv - Biochemistry 2024Quote: ... adding 3 ml of ice-cold Tris buffer and by filtrating the PLs through 0.45 μm pore-size membrane filters (Sartorius Stedim Biotech). Filters were washed twice with 3 ml of Tris buffer and allowed to dry ...
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bioRxiv - Bioengineering 2024Quote: ... and the remaining supernatant was further concentrated using a 100 kDa Vivaspin 500 filter (Sartorius). The crude small EV suspension was then purified with SEC as previously described.
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bioRxiv - Bioengineering 2024Quote: ... and the resulting supernatant was concentrated using a 100 kDa MWCO Vivaspin 20 filter (Sartorius) at 3,000 ×g to a volume of ∼4 ml ...
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bioRxiv - Bioengineering 2024Quote: ... The purified protein from elutions containing the fewest contaminating proteins (typically elution steps 3-5) were concentrated in 1× PBS using a 5 kDa cut-off Vivaspin Centrifugal Concentrator (Sartorius, Oakville, Ontario, Canada). Protein concentrations were determined using the micro-BCA assay (Thermo Fisher Scientific ...
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bioRxiv - Bioengineering 2024Quote: ... After 24 hrs supernatant was collected and concentrated by the Vivaspin-6 5 kDa MWCO filters (Sartorius VS06S1). Cells were lysed in 20 mM Tris-HCl 7.4 pH at 4 °C for 1 hr ...
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bioRxiv - Cancer Biology 2024Quote: ... filtered with a 0.45µm filter (Sartorius), and stored at −80°C ...
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bioRxiv - Biophysics 2024Quote: ... The 50 ml containing 6-His-AdhE was concentrated to a final volume of 10.5 ml using a Vivaspin 20 centrifugal concentrator (Sartorius) with a molecular weight cut-off of 30 kDa and then dialysed against buffer A ...
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bioRxiv - Bioengineering 2024Quote: ... the refolded protein was concentrated using a VIVAFLOW50 system (Sartorius). The concentrated sample was filtrated with 0.22 µm membrane filter ...
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bioRxiv - Bioengineering 2024Quote: The three-step batch cocultivation was done by using Biostat B Plus bioreactor (Sartorius, Germany) equipped with pH-meter (Hamilton ...
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bioRxiv - Bioengineering 2024Quote: ... and arginine (pH 6) to a final concentration of ≥200 mg/mL using the Ambr Crossflow system (Sartorius, Germany).
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bioRxiv - Bioengineering 2024Quote: ... A D-Optimal design with quantitative and qualitative factors was created with MODDE Pro software version 12.1 (Sartorius AG, Göttingen, Germany) (Supplemental Table 1) ...
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bioRxiv - Cancer Biology 2024Quote: ... All cell lines tested negative for mycoplasma contamination using the Microsart AMP Mycoplasma Kit (Sartorius).
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bioRxiv - Cancer Biology 2024Quote: ... Cells were then incubated in an Incucyte S3 Live Cell Analysis Instrument (Sartorius) for six days ...
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bioRxiv - Cancer Biology 2024Quote: ... Glioma cells were stably transduced with a lentiviral nuclear red fluorescent protein under the EF1a promotor (Sartorius, cat. 4476, Göttingen, Germany) for visualization in live imaging assays ...
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bioRxiv - Cell Biology 2024Quote: ... Cytotoxicity and viability were measured using an Incucyte SX5 Live-Cell Analysis System (Sartorius). Images were acquired at regular intervals under 10x magnification using brightfield phase contrast and 100ms exposure of 460nm excitation ...
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bioRxiv - Molecular Biology 2024Quote: ... and Octet NTA biosensors (Sartorius, Göttingen, Germany). HEL (1 µM ...
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bioRxiv - Molecular Biology 2024Quote: ... Acquired data were analysed with the Incucyte® Neurotrack Analysis Software Module (Sartorius) considering the average value of the two images taken for each of the nine technical replicates for all the three biological replicates.
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bioRxiv - Microbiology 2024Quote: U2OS-53PB1-GFP and U2OS-NBS1-GFP cells were imaged using the IncuCyte S3 Live-Cell Analysis Instrument (Sartorius). Mean Fluorescence Intensity (MFI ...
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bioRxiv - Biochemistry 2024Quote: A Biostat A fermentor (Sartorius Stedim Systems GmbH, Guxhagen, Germany) was used to measure population-synchronized metabolic oscillations in continuous cultures with respect to the yeast metabolic cycle [30] ...
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bioRxiv - Biochemistry 2024Quote: Affinity of recombinant RBDs towards the purified ectodomain of TMPRSS2S441Awas assessed in real-time using a bio-layer interferometry Octet-R8 device (Sartorius). Nickel-NTA capture sensors (Sartorius ...
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bioRxiv - Biochemistry 2024Quote: ... Nickel-NTA capture sensors (Sartorius) were loaded for 10 min at 1,000 rpm shaking speed with the different RBDs at 400 nM in PBS ...
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bioRxiv - Biochemistry 2024Quote: ... was immobilized on Octet Amine Reactive 2nd Generation (AR2G) Biosensors (Sartorius) at a concentration of 200 nM in a 10 mM sodium acetate buffer (pH 5.0 ...
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bioRxiv - Biochemistry 2024Quote: ... The refolded solution was concentrated by TFF using a Vivaflow 50R Crossflow Cassette (Sartorius, Göttingen, Germany) and applied onto a preparative size-exclusion chromatography column (Superdex 75 10/300 GL ...
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bioRxiv - Biochemistry 2024Quote: The epitope binning assay was conducted using the Octet RED 96 system (Sartorius). Anti-IFNγ antibody (ab180547 ...
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bioRxiv - Biophysics 2024Quote: ... The acquired raw data for the binding of anti-TcPOP mAbs with TcPOP were processed and globally fit to a 1:1 binding model with Octet Analysis Studio 12.2.2.26 Software (Sartorius). The binding kinetics measurements were carried out in three replicates ...