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Citations for BioLegend :
101 - 150 of 4397 citations for Urothelial Cell Culture Supplements since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Synthetic Biology 2023Quote: ... the cultures were stained for flow cytometry using anti-CD69-PE/Cy7 (1:150) (BioLegend, Cat.#310912) (Y780/60-A) ...
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bioRxiv - Immunology 2021Quote: IL-21 concentration from tonsil mono-cultures was measured using Human IL-21 ELISA MAX Deluxe kit (Biolegend) according to manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2019Quote: ... IL-6 levels of culture supernatants were determined using the LEGENDplex™ Human Inflammation Panel I (BioLegend, 740809), according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2020Quote: ... and taken in culture in the presence of 1μg/ml anti-CD40 antibody (102901, Biolegend, San Diego CA), 25ng/ml IL-4 (404-ML-025 ...
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bioRxiv - Immunology 2019Quote: ... Co-culture supernatants were assayed in duplicate for IgG1 production using the Legendplex Human Immunoglobulin Isotyping Panel (Biolegend) and data acquired on a BD Symphony A5 cytometer ...
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bioRxiv - Immunology 2022Quote: ... culture media was removed and replaced with media containing either 50 ng/mL interleukin 4 (IL-4) (BioLegend) for 48 hr to generate M2 macrophages or 50 ng/mL interferon-gamma (IFN-γ ...
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bioRxiv - Immunology 2023Quote: ... and plated in TRM-like culture media with 10 ng/mL mouse IL-7 (Biolegend Catalog No. 577804) and 10 ng/mL human TGFβ1 (Fisher Scientific PHG9214 ...
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bioRxiv - Immunology 2023Quote: Cytokine analysis from co-culture supernatants was performed with Human CD8/NK Panel (13-plex) Cat#741065 (BioLegend) following the manufacturer’s protocol.
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bioRxiv - Immunology 2019Quote: ... or with culture medium only in the presence of 5¼g/ml Brefeldin A (Biolegend, San Diego, CA, USA) before being submitted to the flow cytometry staining process.
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bioRxiv - Immunology 2022Quote: ... Also added to the culture with the CTLs was anti-CD107a-PE (1:20, BD, or 1:200, Biolegend), GolgiStop (1:800 ...
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bioRxiv - Microbiology 2020Quote: ... TNF-α and IL-2 levels were quantified in co-culture supernatants by ELISA (BioLegend, San Diego, CA, USA).
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bioRxiv - Immunology 2022Quote: ... murine cells were restimulated after 72h of culture with 10nM SIINFEKL257-264 in the presence of Brefeldin A (5µg/ml; Biolegend) for 4h ...
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bioRxiv - Immunology 2023Quote: ... and IL-22 were measured in the culture supernatants using the LEGENDPlex Mouse T Helper Cytokine Panel kit (BioLegend) following manufacturer’s instructions.
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bioRxiv - Immunology 2020Quote: Cytokine concentrations in supernatants of in vitro cultures were analyzed by the LegendPlex Mouse Th Cytokine Panel (13-plex) (BioLegend) according to the manufacturer’s instructions and analyzed on a FACS LSR II (BD Biosciences).
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bioRxiv - Immunology 2022Quote: ... TCR stimulation was evaluated by measuring IL-2 concentration in culture supernatant using an IL2 ELISA kit (#423001, #423501, #431001 and #421101, BioLegend).
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bioRxiv - Genomics 2020Quote: ... Cytokine concentrations in super-natants of in vitro cultures were analyzed by the LegendPlex Mouse Th Cytokine Panel (13-plex) (BioLegend) according to the manufacturer’s instructions and analyzed on a FACS LSR II (BD Biosciences).
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bioRxiv - Physiology 2019Quote: ... Cell culture supernatant was collected and frozen for IL-2 analysis using LEGENDplex™ technology according to the manufacturer’s instructions (Biolegend).
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bioRxiv - Immunology 2020Quote: ... Phenotypes of B cells before and after 6 days of culture were determined by flow cytometric analysis using mouse monoclonal antibody clones: CD19 PECy7 HIB19 (Biolegend), CD27 BV421 O323 (Biolegend) ...
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bioRxiv - Immunology 2021Quote: ... Cell viability staining was routinely performed for plate stimulations and U87 co-culture using fixable violet or near-infrared viability dyes (Zombie UV fixable viability kit [Biolegend] ...
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bioRxiv - Immunology 2020Quote: ... Cell viability staining was routinely performed for plate stimulations and U87 co-culture using fixable violet or near-infrared viability dyes (Zombie UV fixable viability kit [Biolegend] ...
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bioRxiv - Immunology 2022Quote: The presence of IL-17A in T-cell culture supernatants and of IL-6 and IL-8 in fibroblast culture supernatants was quantified by ELISA according to manufacturer instructions (deluxe or standard kits, respectively, all BioLegend). Plates were read at 450 nm using a Spark 10M (Tecan) ...
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bioRxiv - Immunology 2023Quote: ... the appropriate number of wells of a flat-bottomed tissue culture plate were coated with 10 μg/mL αCD3 (Biolegend) in PBS for 3h at 37°C ...
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bioRxiv - Bioengineering 2023Quote: IFNψ was measured from the supernatants of the co-culture experiments (TCE / CAR) using the colorimetric ELISA MAX Standard Set Human IFNψ kit (BioLegend) according to the manufacturer’s instruction ...
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bioRxiv - Immunology 2023Quote: Cytokines secreted in the culture supernatant of cytokine-stimulated sip-T were measured by a LEGENDplex Human CD8/NK Panel kit (BioLegend) following manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2023Quote: ... 2×106 bone marrow cells were cultured at 37°C in 10ml bmDC culture medium (RPMI 1640 medium with 10% FBS, penicillin/streptomycin and 2-Mercaptoethanol) with 40ng/mL GM-CSF (BioLegend). At day 3 ...
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bioRxiv - Bioengineering 2023Quote: ... The culture supernatant was collected and the Th2 cytokines were measured using the LEGENDplex™ MU Th cytokine panel (Biolegend) according to the manufacturer’s protocol.
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bioRxiv - Bioengineering 2023Quote: ... The culture supernatant was collected and the Th2 cytokines were measured using a LEGENDplex™ mouse Th cytokine assay (Biolegend) according to the manufacturer’s protocol.
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bioRxiv - Immunology 2024Quote: ... The following antibodies were used to stain human pDCs either from PBMCs or after purification and culture as described in the text: CD3 (UCHT1, BioLegend), CD14 (HCD14 ...
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bioRxiv - Cancer Biology 2019Quote: ... The cells were washed with HBSS twice by centrifugation at 250 x g for 8 min and then suspended in BMDC culture medium (RPMI-1640 medium [Thermo Fisher Scientific] containing 10% FBS, 20 ng/ml recombinant mouse GM-CSF [BioLegend] ...
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bioRxiv - Immunology 2022Quote: ... Global inflammatory cytokine analysis of supernatants of adipose tissue explant cultures and serum were performed using LEGENDPlex™ Mouse Inflammation Panel (740446, Biolegend). TNFα and IL-10 levels were measured by TNFα Mouse Uncoated ELISA Kit (88-7324-86 ...
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bioRxiv - Immunology 2020Quote: Supernatants of stimulated T cells were harvested on day 6 of culture and analyzed using the LEGENDplex™ human Thelper 1/2/9/17 multiplex kit (BioLegend). Beads were acquired on a BD Canto II (BD Bioscience ...
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bioRxiv - Immunology 2020Quote: ... TNFα and IL-23 expression in the culture medium was examined using an ELISA (Invitrogen for TNFα Biolegend for IL-23).
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bioRxiv - Immunology 2022Quote: ... IL-2 and IL-7 were removed from ILC2 culture and additionally cultured for 4 hr before restimulation with IL-33 (Biolegend, #580508) plus NMU (R&D systems ...
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bioRxiv - Immunology 2022Quote: ... T cells at 1×106/ml were kept in culture withdraw of cytokines for 8 hours before stimulated with 1µg/ml plate-coated anti-mouse CD3e (Biolegend, Cat.100340) and 1µg/ml soluble anti-mouse CD28 (Biolegend ...
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bioRxiv - Cancer Biology 2022Quote: ... LEPs from co-cultures were separated after 10 days for gene expression qPCR analysis by FACS using anti-human CD133-PE (BioLegend, clone7) and anti-human CD271-APC (BioLegend ...
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bioRxiv - Immunology 2023Quote: ... Purified naïve CD8 T cells were seeded on non-tissue culture treated 24 well plates coated with 2mg/ml αCD3ε (Ultra LEAF© purified, clone145-2C11, Biolegend) at 2x105 cells per well ...
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bioRxiv - Cancer Biology 2023Quote: ... co-culture samples were collected at 120h and stained for live/dead fixable Aqua and CD3 (Clone HIT3a, Biolegend, Cat# 300306) in FACS buffer ...
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bioRxiv - Immunology 2024Quote: Murine DCs were differentiated from isolated bone marrow via culture in RPMI + 10% FCS 1% Pen/Strep and 1% Amino Acids containing 20 ng/ml GMCSF (Biolegend, #576306) on silicone hydrogels with different stiffnesses ...
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bioRxiv - Microbiology 2022Quote: ... Quantification of cytokine and chemokine levels in culture media was performed using the LEGENDPlex kit from BioLegend (human anti-virus response panel), according to the manufacturer’s recommendations.
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bioRxiv - Genomics 2019Quote: ... clones that survived after 3-4 months of culture were washed with PBS and stained with FITC anti-HLA-DQ (Biolegend, Clone: HLADQ1) for 30 minutes on ice and cell surface expression assessed by flow cytometry ...
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bioRxiv - Physiology 2023Quote: Concentrations of tumour necrosis factor (TNF)-α from tissue culture supernatants post- stimulation were measured using ELISA MAX Standard Set Mouse TNF-α kit (Biolegend) according to the manufacturer’s protocol.
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bioRxiv - Immunology 2023Quote: ... Co-culture supernatant was collected following completion of culture and analyzed for IL-2 using the ELISA MAX™ Deluxe Set for human IL-2 (Biolegend, 431804) at a dilution of 1:5.
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bioRxiv - Bioengineering 2024Quote: The supernatant of CAR T and cancer co-culture systems was measured for their expression of cytokines using the LegendPlex Human Essential Immune Response Panel (Cat # 740930, BioLegend, San Diego, CA) (IL-4 ...
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bioRxiv - Immunology 2022Quote: ... cells were stimulated using Cell Activation cocktail (Biolegend) for 4h at 37°C in RPMI containing 10% FBS ...
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bioRxiv - Immunology 2021Quote: Peripheral blood mononuclear cells (Veri-Cells PBMC, BioLegend) were stained with each novel fluorochrome for 30 minutes and fixed with various fixatives according to the manufacturer’s instructions – Cell Staining Buffer (PBS + 10% Fetal Bovine Serum (FBS)) ...
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bioRxiv - Physiology 2023Quote: ... Cells were washed with cell staining buffer (Biolegend), and non-specific binding was blocked with TruStain FcX Plus (Biolegend ...
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bioRxiv - Microbiology 2024Quote: ... Cells were washed with Cell Staining Buffer (BioLegend) and stained with a cocktail of surface antibodies for 20 minutes at 4°C ...
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bioRxiv - Immunology 2024Quote: ... cells were resuspended in Cell Staining Buffer (BioLegend) containing Fc receptor blocker (Miltenyi Biotec ...
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bioRxiv - Immunology 2024Quote: ... Isolated CD4+ T-cells were either maintained in a naïve state by seeding in complete growth medium alone or underwent TH1-activation in tissue culture flasks pre-coated with anti-human CD3 antibody (10 µg/mL) (BioLegend, San Diego, CA, US) in complete medium supplemented with anti-human CD28 antibody (5 µg/mL ...
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bioRxiv - Bioengineering 2021Quote: ... Cell pellets were resuspended in cell staining buffer with zombie aqua cell viability stain (BioLegend), were incubated at 4 °C for 15 minutes ...