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Citations for BioLegend :
401 - 450 of 4397 citations for Urothelial Cell Culture Supplements since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2023Quote: ... CD4+ T cells were enriched using a MojoSort™ T cell isolation kit (BioLegend, Cat no. 480006) following the manufacturer’s protocol.
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bioRxiv - Cancer Biology 2023Quote: ... one million cells were harvested 3 days after transduction and washed twice with cell staining buffer (Biolegend). Cells were resuspended in 100μL of Cell Staining Buffer (Biolegend ...
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bioRxiv - Cancer Biology 2023Quote: Cell death was determined using flow cytometry through the quantification of cell surface annexin V-APC (Biolegend) and propidium iodide (PI ...
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bioRxiv - Genomics 2022Quote: ... cells were activated at ∼2-4M cells/ml for 8 hrs using CD3 antibody (Biolegend clone OKT3) coated tissue culture plates ...
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bioRxiv - Immunology 2023Quote: CD4+ T cells were isolated from the spleen using MojoSort Mouse CD4 T cell isolation kit (BioLegend) and sorted according to their expression of CD44 and NKG2D ...
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bioRxiv - Immunology 2023Quote: ... B cells and monocytes were isolated from PBMCs (AllCells) using Mojosort magnetic cell separation system from Biolegend via CD3 positivity (Biolegend ...
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bioRxiv - Cancer Biology 2024Quote: ... T cells were isolated using the MojoSort Human CD3 T Cell Isolation Kit (cat. no. 480021, Biolegend) and EasySep Magnet (cat ...
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bioRxiv - Cancer Biology 2024Quote: ... Cells were washed 2 times in 1x Perm/Wash buffer and resuspended in Cell Staining Buffer (Biolegend) prior to flow cytometry analysis ...
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bioRxiv - Cancer Biology 2024Quote: ... and the cells were counted and resuspended in 1 ml of cell staining buffer (CSB) (BioLegend, 420201) for each sample ...
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bioRxiv - Immunology 2024Quote: ... T cell activation was assessed by incubating cells with fluorescein isothiocyanate-conjugated anti-human CD8 (Biolegend, 100705) and phycoerythrin-conjugated anti-human CD25 (Biolegend ...
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bioRxiv - Cell Biology 2020Quote: ... naïve CD4+ T cells isolated from OTII/CD45.2 TCR transgenic donor mice using naïve CD4+ mouse T cell isolation kit (Biolegend) were labeled with CFSE ...
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bioRxiv - Immunology 2021Quote: Splenic B cells were isolated from bone marrow chimeras (MojoSort™ Mouse Pan B Cell Isolation Kit; Biolegend). Control and WNK1-deficient B cells were labelled with CellTracker blue (CMAC ...
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bioRxiv - Immunology 2021Quote: Freshly thawed decidual immune cells (1-2 10e6 cells) were incubated with Fc blocker (Human TruStain FcX, Biolegend) in PBS with 1% BSA for 10 minutes at 4C and then surface stained with CD45-FITC (HI30 ...
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bioRxiv - Genomics 2020Quote: ... cells were resuspended in ice-cold PBS with 1:500 dilution of Zombi-NIR dead cell stain (Biolegend) and incubated for 15 minutes in the dark on ice ...
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bioRxiv - Neuroscience 2019Quote: ... The cell pellet was then stained with antibodies to microglial cell surface markers (CD11b-BV650, 1:200 Biolegend, #141723 ...
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bioRxiv - Bioengineering 2020Quote: ... The non-macrophages cell fraction was then incubated in mouse CD4+ T cell isolation biotin-antiboy cocktail (BioLegend) and sorted ...
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bioRxiv - Immunology 2022Quote: ... cells were stained with antibodies for cell surface markers in FWB plus fixable viability dye (Zombie Red; BioLegend), then fixed and permeabilized using the BrdU APC kit (BD Biosciences ...
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bioRxiv - Immunology 2022Quote: ... naïve CD4+ T cells isolated from OTII/CD45.2 TCR transgenic donor mice using naïve CD4+ mouse T cell isolation kit (Biolegend) were labeled with CFSE ...
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bioRxiv - Neuroscience 2022Quote: ... Dead cells were excluded by labelling with the cell viability dye Zombie Aqua (2 ng/ml, BioLegend, 423101) that was added to the single cell suspension before sorting ...
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bioRxiv - Immunology 2022Quote: ... Proliferation was determined by labeling T cells with the Tag-IT Violet Proliferation Cell Tracking Dye (Biolegend, #425101), according to manufacturer instructions ...
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bioRxiv - Cancer Biology 2019Quote: Cells were detached using 2mM EDTA in PBS and cell surface expression of CD49b was measured (Biolegend 359310). Annexin V staining was performed using FITC Annexin V apoptosis Detection Kit (BD Biosciences 556547).
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bioRxiv - Immunology 2019Quote: ... Cell surface phenotyping of transduced cells was determined by staining with BV785-conjugated anti-human CD3 (UCHT1, BioLegend), BV605-conjugated anti-human CD4 (OKT-4 ...
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bioRxiv - Biochemistry 2019Quote: ... cells were lysed and the detergent-solubilized cell lysates were incubated with an anti-HA antibody (BioLegend, 901503) at 4 °C overnight ...
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bioRxiv - Immunology 2020Quote: For mouse lung cell staining: cell suspensions were incubated with purified anti-mouse CD16/32 (clone 93, BioLegend) for 10 minutes at 4°C ...
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bioRxiv - Cancer Biology 2021Quote: ... cells were stained with the appropriate surface markers [dead cell marker-Zombie Aqua™ Fixable Viability Kit (Biolegend)] and CD8 (APC conjugated ...
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bioRxiv - Microbiology 2020Quote: ... the GFP+ cells were quantified by flow cytometry with pacific blue viability dye to exclude dead cells (Biolegend).
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bioRxiv - Immunology 2021Quote: ... and CD3+ cells were depleted from the donor cells using a biotinylated anti-mouse CD3e mAb (BioLegend; 100304) and the Miltenyi MACS® MicroBead (Miltenyi ...
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bioRxiv - Immunology 2021Quote: ... Naive P14 Thy1.1+ Il2ra−/− cells were isolated from 4-5 week old mice by depleting CD44hi cells (Biolegend biotin anti-mouse/human CD44 ...
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bioRxiv - Immunology 2020Quote: ... Cells were placed in V-bottom plates and stimulated with 500X Cell Activation Cocktail with Brefeldin A (Biolegend) in IMDM medium supplemented with 8% FCS ...
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bioRxiv - Immunology 2021Quote: ... CD3+ cell enrichment was achieved by MojoSort negative selection using MojoSort mouse CD3+ T-cell Isolation Kit (BioLegend) according to manufacturer’s instructions ...
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bioRxiv - Immunology 2022Quote: CD4+ T cells (naïve or total) were enriched with naïve or total CD4+ T cell negative enrichment kits (Stemcell, Biolegend) from the spleens of female ...
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bioRxiv - Immunology 2022Quote: ... Naïve CD4 T cells were presorted with the MojoSort™ Mouse CD4 Naïve T cell Isolation kit (BioLegend) based on provided directions ...
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bioRxiv - Cell Biology 2023Quote: ... and T cells were isolated from the spleen using the MojoSortTM Mouse CD3 T cell Isolation Kit (Biolegend). Experiments where purified T cells were cultured for longer than 24 hours ...
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bioRxiv - Microbiology 2023Quote: ... Blood or tonsil CD4+ T cells were isolated using a human CD4+ T cell isolation kit (BioLegend #480010). Purified CD4+ T cells were used without stimulation or co-stimulated with plate-bound CD3 and soluble CD28 antibodies in the presence of 20 ng/ml IL-2 for three days ...
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bioRxiv - Cell Biology 2022Quote: ... Both cell fractions were stained with cell hashing antibodies (1:1000, #2 for heart, #3 for blood, Biolegend) and Propidium iodide (1:100 ...
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bioRxiv - Immunology 2023Quote: ... CD8+ T cells were isolated by negative selection using the MojoSort Mouse CD8+ T cell isolation kit (BioLegend) and cultivated as described above ...
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bioRxiv - Genomics 2023Quote: ... 500,000 cells from bulk and CD45+ enriched fraction were individually re-suspended in Cell Staining Buffer (420201, Biolegend) and blocked with 5 µL Human TruStain FcX™ (422301 ...
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bioRxiv - Genomics 2023Quote: ... CD4+ T-cells were then isolated using the human CD4+ T-cell MojoSort negative magnetic selection kit (Biolegend). Enriched CD4+ T-cells were then rested overnight in a 37°C 5% CO2 incubator in complete RPMI (RPMI1640 ...
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bioRxiv - Immunology 2023Quote: ... Cells were then washed by centrifugation (250g for 10 min) with FACS cells staining buffer (Cat# 420201, BioLegend). Cells were next treated with human 5µl/tube TruStain FcX™ (Fc receptor blocking solution ...
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bioRxiv - Cancer Biology 2023Quote: ... transgenic T cells were labeled with Tag-it Violet Proliferation and Cell Tracking Dye (Cat No. 425101 Biolegend). The frequency of SMARTA cells that proliferated during culture was determined as described by Gett et al ...
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bioRxiv - Cancer Biology 2023Quote: ... harvested cells were incubated in Roswell Park Memorial Institute medium with cell activation cocktail with brefeldin A (Biolegend) for 4.5 hours at 37°C ...
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bioRxiv - Microbiology 2023Quote: Naïve B cells were isolated from spleens by immunomagnetic depletion using MojoSort Pan B cell Isolation Kit (Biolegend) and cultured in RPMI supplemented with L-glutamine ...
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Downregulation of Let-7 miRNA promotes Tc17 differentiation and emphysema via de-repression of RORγtbioRxiv - Immunology 2023Quote: CD8+ naïve T cells were isolated from spleen using Mojosort Mouse CD8 Naïve T cell isolation Kit (Biolegend) and adjusted to a concentration of 1.0x106 cells/mL ...
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bioRxiv - Immunology 2024Quote: ... cells were stained with fluorophore-labelled antibodies targeting the following cell surface markers: APC-SiglecH (Clone 551, BioLegend), APC-Cy7-CD11b (Clone M1/70 ...
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bioRxiv - Immunology 2024Quote: ... single-cell suspensions were stimulated with cell activation cocktail (including phorbol 12-myristate 13-acetate and Ionomycin) (BioLegend) in the presence of Golgi Stop (monensin ...
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bioRxiv - Microbiology 2021Quote: ... cell stimulation cocktail (containing PMA-Ionomycin, Biolegend), 1μg/ml SARS-CoV-2 S peptide pool ...
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Mapping of the autophagosomal degradome identifies IL-7Rα as key cargo in proliferating CD4+ T-cellsbioRxiv - Immunology 2021Quote: ... cells were fixed with Fixation Buffer (BioLegend) in the dark for 20 min at room temperature after surface staining ...
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Mapping of the autophagosomal degradome identifies IL-7Rα as key cargo in proliferating CD4+ T-cellsbioRxiv - Immunology 2021Quote: ... cells were stained with AF647-Streptavidin (BioLegend), α-CD4 BV605 GK1.5 (BioLegend ...
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bioRxiv - Genomics 2020Quote: ... Cells were fixed in fixation buffer (Biolegend) and analyzed on an Aurora Spectral Analyzer (Cytek Biosciences) ...
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bioRxiv - Immunology 2021Quote: Cells were incubated with Human FcX (Biolegend) to prevent non-specific antibody binding ...