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Citations for BioLegend :
51 - 100 of 4397 citations for Urothelial Cell Culture Supplements since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2023Quote: ... Supernatants were collected at d5 of the culture for the analysis of T cell cytokine production using a combination of commercially available antibodies (Biolegend). Briefly ...
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bioRxiv - Immunology 2024Quote: Secretion of soluble cytokines and chemokines were assayed from cell-free culture supernatants of in vitro stimulated neutrophils and monocytes using bead-based immunoassays (LegendPlex, Biolegend). Two assays were used allowing the quantification of IL-1β ...
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bioRxiv - Immunology 2023Quote: ... 106 of the CTV labeled T cells were stimulated in vitro for 3 or 5 days in cultures containing 100ng/ml IL-15 (BioLegend) or a combination of 100ng/ml IL-7 (BioLegend ...
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bioRxiv - Immunology 2020Quote: ... Supernatant culture cytokine levels for IL-5 (Biolegend) and IL-13 (eBioscience ...
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bioRxiv - Genomics 2019Quote: ... Cultures were then stained with F4/80 (Biolegend) to ensure that ~95% of the culture had differentiated into macrophages ...
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bioRxiv - Immunology 2024Quote: ... co-cultures were treated with Brefeldin A (Biolegend) for 5h and harvested for intracellular cytokine staining with the FOXP3/Transcription Factor Staining Buffer Set (eBioscience ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... The culture supernatants of stimulated cells were collected for cytokine (i.e., IL-4 and IFN-γ) assays using ELISA kits (BioLegend, CA).
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bioRxiv - Immunology 2021Quote: ... Any contaminating myeloid cells were removed from cultures prior to experimental setup by negative selection using a biotinylated anti-CD11b antibody (Biolegend, #101204) followed by subsequent Streptavidin MicroBeads (Miltenyi Biotec ...
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bioRxiv - Cancer Biology 2022Quote: Cytokine analysis was performed on cell culture supernatants using LEGENDplex™ HU Essential Immune Response Panel (740930, Biolegend, San Diego, CA) according to the manufacturer’s instructions (65).
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bioRxiv - Immunology 2023Quote: ... Ex vivo CD8+ T cells or in vitro stimulated (IVS) cultures were labeled with the Zombie Aqua™ Fixable Viability Kit (BioLegend) to exclude dead cells in all flow cytometry experiments ...
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bioRxiv - Cancer Biology 2021Quote: ... The cells and the culture media were collected and stained with annexin V-FITC mAb (BioLegend, San Diego, CA, Cat. No. 640906) in binding buffer (10 mM HEPES ...
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bioRxiv - Cancer Biology 2022Quote: ... About 5×106 bone marrow cells were plated into 100mm non-tissue culture treated plates containing 20ng/ml GMCSF (Biolegend, Cat# 640920). On day 3 5ml of fresh media containing 20ng/ml of GMCSF was added to the cultures ...
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bioRxiv - Immunology 2023Quote: ... CAR-T cells (1×105 cells/well) and Nalm6-GD2 (1×105 cells/well) were plated in Nalm6-GD2 culture medium containing 1×Brefeldin A (Biolegend, Cat# 420601) for 5-7h ...
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bioRxiv - Immunology 2022Quote: ... culture media containing 20ng/ml of recombinant CCL20 (Biolegend) or conditioned media (either adjacent or lesion) ...
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bioRxiv - Immunology 2020Quote: Concentrations of IL-1β and IL-6 in cell culture supernatants or blood serum were measured using cytokine-specific ELISAs (BioLegend ELISA Max kits), according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2022Quote: Cytokine levels in the plasma and cell culture medium were detected using the LEGENDplex™ Human Th Panel (BioLegend, San Diego, CA, USA) containing 13 human antibodies targeting IL-5 ...
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bioRxiv - Immunology 2021Quote: ... Cultures with isotype-matched antibody (BioLegend, San Diego, CA, USA) served as controls for determining the specificity in these blocking assays.
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bioRxiv - Cancer Biology 2021Quote: ... suspended in culture medium containing IL-2 (10ng/ml; BioLegend). Cells were directly stained with CFSE (Cayman Chemical ...
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bioRxiv - Neuroscience 2021Quote: ... Cultures were then incubated on ice with mouse anti-HA (BioLegend) diluted 1:500 in PBS-MC for 30 min ...
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bioRxiv - Neuroscience 2020Quote: ... and for co-cultures mouse anti-TUBB3 1:1000 (Biolegend 801201), rabbit anti-VGLUT1 1:300 (Sigma ...
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bioRxiv - Pathology 2023Quote: ... Neutrophils were maintained 4 hours in culture with Brefeldin A (Biolegend) to stop exocytosis.
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bioRxiv - Immunology 2023Quote: ... in the culture medium containing 1×Brefeldin A (Biolegend, Cat#420601) in a 96-well plate for 5-7h ...
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bioRxiv - Microbiology 2023Quote: ... The culture supernatant was used for IL-1β ELISA (BioLegend #437004).
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bioRxiv - Cell Biology 2020Quote: ... mouse anti-Myc (1:2, 9E10 Hybridoma culture supernatant, 1:1000 Biolegend); rabbit anti pRab10 (1:1000 ...
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bioRxiv - Cell Biology 2021Quote: Breast tumor MDA-453 were lifted from culture dish using Accutase (Biolegend) and incubated with human B2-IgG or B2-Fab in 100 μL PBS and 0.1% bovine serum albumin for 45min at 4 °C ...
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bioRxiv - Immunology 2021Quote: ... cultures were additionally supplemented with 5ng/mL recombinant mouse IL-7 (Biolegend). Scid.adh.2c2 cells were cultured in IMDM supplemented with 10% FBS (Hyclone) ...
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bioRxiv - Immunology 2023Quote: ... the culture supernatants were evaluated using the ELISA MAX assay kit (BioLegend), according to the manufacturer’s specifications.
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bioRxiv - Cancer Biology 2023Quote: ... Co-culture samples were stained with Zombie Violet Fixable Viability Kit (BioLegend) followed by anti-CD69 APC antibody (FN50 ...
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bioRxiv - Immunology 2023Quote: ... Culture supernatants were collected and analyzed for IL-2 by ELISA (BioLegend).
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bioRxiv - Bioengineering 2024Quote: ... The induced culture was stained with anti-HA-APC (cat. 901523, BioLegend) and tetramerized streptavidin--PE ...
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bioRxiv - Immunology 2023Quote: ... Culture medium is supplemented with 1000U/well murine GM-CSF (Biolegend, Cat#576302) and a predetermined dose of P815-IL4 supernatant ...
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bioRxiv - Cancer Biology 2023Quote: ... and the co-culture was analyzed using human CD3 (FITC and HIT3a, BioLegend), CD8 (APC and SK1 ...
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bioRxiv - Cancer Biology 2021Quote: ... Co-cultures were harvested and then stained with fixed viability dye (Zombie Aqua, Biolegend) and fluorochrome-labelled T-cell-specific antibodies targeting CD5 (clone 53-7.3 ...
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bioRxiv - Immunology 2019Quote: ... Culture supernatants were collected and analyzed for IFNg and IL-2 by ELISA (BioLegend).
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bioRxiv - Immunology 2020Quote: ... when culture purity was assessed by flow cytometry and expression of CD106 (Biolegend, 105722), CD54 (Biolegend ...
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bioRxiv - Immunology 2020Quote: ... Supernatants were harvested 48 hours after culture and analyzed for IFNβ by ELISA (Biolegend) according to manufacturer protocol.
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bioRxiv - Cancer Biology 2024Quote: ... the culture was supplemented with 0.5X concentrations of both brefeldin A and monensin (Biolegend) to facilitate cytokine retention within the cells ...
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bioRxiv - Immunology 2024Quote: The concentrations of cytokines in culture supernatants were quantified using ELISA Kits (Invitrogen, Biolegend). The ELISA procedure was carried out strictly following the manufacturer’s instructions ...
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bioRxiv - Immunology 2022Quote: ... tissue culture plates were coated with 5 μg/mL of anti-human CD162 (KPL, Biolegend). On day three of activation ...
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bioRxiv - Immunology 2019Quote: ... Culture supernatants were assayed in duplicate or triplicate using a human TNF ELISA MAX (Biolegend). In samples where undiluted supernatants fell outside the standard curve ...
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bioRxiv - Genomics 2020Quote: ... and IL-10 in culture supernatants were measured using ELISA Max Deluxe were from Biolegend® (CA ...
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bioRxiv - Neuroscience 2020Quote: ... neuronal cultures were treated with 200 ng/mL recombinant human NRG1 (BioLegend, Cat. No. 551904) for 48 hrs ...
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bioRxiv - Immunology 2020Quote: ... tissue-culture plates were coated with 5 μg/mL purified anti-CD3 (clone HIT3a, Biolegend) at 4°C overnight and subsequently washed twice with 1X PBS ...
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bioRxiv - Immunology 2022Quote: ... mucus scrapes and culture supernatants was carried out using commercially available LEGENDplex Multiplex Assays (BioLegend), murine serum auto-antibodies were quantified using the respective commercially available Alpha Diagnostics Intl ...
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bioRxiv - Bioengineering 2024Quote: ... Secreted IFNγ concentrations in the conditioned culture media were measured using an ELISA kit (BioLegend).
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bioRxiv - Immunology 2023Quote: ... Cultures were treated with anti-human CD2 mAb (10µg/mL; Cat# 300240; Clone: RPA-2.10; Biolegend) or IgG (10 µg/mL ...
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bioRxiv - Synthetic Biology 2023Quote: ... cultures were stained for flow cytometry with anti-CD69-PE/Cy7 (1:150) (BioLegend, Cat.#310912) (Y780/60-A) ...
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bioRxiv - Cancer Biology 2022Quote: ... and then plated in a culture plate pre-coated with 10 µg/mL anti-CD3Ɛ antibody (Biolegend). Culture medium was exchanged to R10 containing IL-2 and the cell density was adjusted to be 0.5 – 1x106 cells/ml every 24 h.
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bioRxiv - Immunology 2020Quote: ... Isolation of murine IEC and ILC1 following co-culture was performed using EpCAM–APC Cy7 (G8.8, BioLegend), CD45-BV510 (30-F11 ...
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bioRxiv - Immunology 2019Quote: ... each well used for culture was incubated with 3 μg/mL anti-CD3 (Biolegend clone 145-2C11) and 3 μg/mL anti-CD28 (Biolegend clone 37.51 ...