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Citations for BioLegend :
4901 - 4950 of 5512 citations for Recombinant Human APCS Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2021Quote: ... permeabilized for 10 min in PBS/0.1% saponin and stained with FITC-conjugated anti-human Perforin (γG9, Biolegend) and PE-conjugated anti-human Perforin antibody (B-D 48 ...
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bioRxiv - Immunology 2022Quote: ... The concentration of CXCL10 in the media was determined using a Human CXCL10 ELISA MAX kit (BioLegend 439904).
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bioRxiv - Cell Biology 2022Quote: ... Cells were incubated with antibodies directly conjugated to fluorescent dyes (anti-human BAFF-R conjugated to FITC, BioLegend, Cat ...
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bioRxiv - Immunology 2022Quote: Secreted cytokines were assessed with LEGENDplex Human Th Panel 12-plex and analysed with Qognit software (both BioLegend). For cytokine secretion by Tregs ...
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bioRxiv - Immunology 2024Quote: ... Following antibodies were used for the extracellular staining according to the manufacturer’s protocol: PerCP/Cyanine5.5 anti-human CD3 Antibody (#300430, BioLegend), Brilliant Violet 421 anti-human CD4 Antibody (#357424 ...
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bioRxiv - Developmental Biology 2024Quote: ... Cells were subsequently stained with CD47-PerCPCy5.5 and CD26-PE antibodies (Anti-human CD47 PerCP/Cy5.5 conjugate, Biolegend, Cat#323110 ...
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bioRxiv - Cancer Biology 2022Quote: ... from healthy human donor Leukopaks and expanded either on anti-CD3 coated plates (+anti-CD28/IL-2, BioLegend), or in the presence of matched donor-derived monocyte-derived dendritic cells (Wölfl and Greenberg ...
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bioRxiv - Genomics 2024Quote: ... incubated with 0.5ug of a unique Cell Hashing antibody (TotalseqTM-A0251 to A0257 anti-human hashtag antibody, BioLegend) for 20 minutes at 4 °C ...
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bioRxiv - Neuroscience 2024Quote: Cytokine levels in saliva were measured twice per sample using the LEGENDplex Human Essential Immune Response panel (BioLegend), as described in previous studies 26,57 ...
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bioRxiv - Immunology 2024Quote: ... Cells were blocked for 15 min with 10 µL of human Fc receptor blocking solution (TruStain FcXTM; BioLegend). To minimize the interaction of polymeric dyes and improve discrete fluorochrome readout ...
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bioRxiv - Immunology 2024Quote: ... and 1 mL 400 mg/mL Pacific Blue-mouse-anti-human CD45 (BioLegend; Cat. No. 304029; clone HI30), 1 mL 50 mg/mL PE/Dazzle594-mouse-anti-human CD16 (BioLegend ...
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bioRxiv - Immunology 2024Quote: ... and 1 mL 70 mg/mL BV421-mouse-anti-human CD103 (Biolegend; Cat. No. 350214; clone Ber-ACT8). After the incubation ...
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bioRxiv - Immunology 2024Quote: ... 100 µL 1:2500 diluted (1% NAP Blocker in PBS) HRP-conjugated anti-human IgG antibody (BioLegend #410902) was added to each well ...
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bioRxiv - Immunology 2024Quote: NK cells were defined as positive for CD56dim using Brilliant Violet 650 conjugated mouse anti-human CD56 (BioLegend) and PerCP/Cyanine5.5 conjugated mouse anti-human CD16 antibody (BioLegend ...
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bioRxiv - Immunology 2023Quote: ... The cells were washed with cold PBS and PE-labeled anti-human IgG Fc secondary antibody (Biolegend, 41070) was added and incubated for 30 min at 4°C ...
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bioRxiv - Immunology 2023Quote: ... The amount of secreted IFN-γ was determined the LEGEND MAX™ Human IFN-γ ELISA Kit (BioLegend) according to the manufacturer’s instructions.
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bioRxiv - Immunology 2023Quote: ... The IcAR-PD1 cell line was stained using PE-conjugated mouse anti-human PD1 antibody (cat: 379210, BioLegend). PE-conjugated mouse IgG2b (cat ...
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bioRxiv - Cancer Biology 2023Quote: ... Cells were then stained with 2.5 μl of PE anti-human E-cadherin (50 μg/ml; Biolegend, 324106) or matched concentration of PE Mouse IgG1 ...
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bioRxiv - Immunology 2022Quote: ... Cytokines in the cell culture supernatants were quantified using Human Th Cytokine Panel (12-plex) (Cat. 741027, BioLegend) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2023Quote: ... Blood or tonsil CD4+ T cells were isolated using a human CD4+ T cell isolation kit (BioLegend #480010). Purified CD4+ T cells were used without stimulation or co-stimulated with plate-bound CD3 and soluble CD28 antibodies in the presence of 20 ng/ml IL-2 for three days ...
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bioRxiv - Cancer Biology 2023Quote: ... untreated AML patients were determined by enzyme-linked immunosorbent assay (ELISA) using an IL21 Human ELISA kit (Biolegend), according to the manufacturer’s instructions.
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bioRxiv - Immunology 2023Quote: ... Cells were pelleted again and resuspended in 100 μl of 1:10 dilution of Human TruStain FcX (Biolegend) in MACS buffer ...
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bioRxiv - Neuroscience 2023Quote: Determination of human α-synuclein concentration in nodose ganglia was performed according to manufacter’s instructions (Biolegend, Cat#844101). The 1:10 diluted samples were run in triplicates and reference standard curve ranged from 6.1 – 1500 pg per mL ...
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bioRxiv - Microbiology 2023Quote: ... fluorescein isothiocyanate-conjugated polyclonal goat anti-human C3b antibody (ICN) and PE-conjugated goat anti-mouse IgG (Biolegend). For the neutrophil killing assays ...
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bioRxiv - Immunology 2023Quote: Surface expression of mouse or human CD47 on murine tumor cell lines was assessed using MIAP301-PE (Biolegend) and CC2C6-APC (Biolegend ...
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bioRxiv - Immunology 2023Quote: Cytokines were quantified using the LEGENDplex Human CD8/NK Panel 13-plex (Cat.No # 740267 Lot B330268) from Biolegend. This is a bead-based immunoassay able to quantify the concentration of different analytes in co-culture supernatants ...
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bioRxiv - Bioengineering 2023Quote: ... the primary monocytes were isolated using a monocyte isolation kit (MojosortTM Human CD14+ Monocytes Isolation Kit, BioLegend®), per the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2023Quote: ... CD34+ cells were further sorted by FACS for CD7+ using anti-human CD7-FITC antibody (CD6-6B7; BioLegend).
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bioRxiv - Genomics 2023Quote: ... CD4+ T-cells were then isolated using the human CD4+ T-cell MojoSort negative magnetic selection kit (Biolegend). Enriched CD4+ T-cells were then rested overnight in a 37°C 5% CO2 incubator in complete RPMI (RPMI1640 ...
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bioRxiv - Immunology 2023Quote: Listed below are the antibodies used for flow cytometric analysis of human cellsurface molecules: ①Purchased from Biolegend(USA): Anti-hCD36-PE(clone 5271).
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bioRxiv - Immunology 2023Quote: ... Cells were next treated with human 5µl/tube TruStain FcX™ (Fc receptor blocking solution, Cat# 422302, BioLegend) and incubated for 30 min at RT ...
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bioRxiv - Immunology 2023Quote: ... both human and murine single cell suspensions were first stained with a viability marker (Fixable Viability Kit, Biolegend) according to manufacturer’s instructions to exclude dead cells ...
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bioRxiv - Immunology 2023Quote: ... 100 µl 1:2500 diluted (1% NAP Blocker in PBS) HRP-conjugated anti-human IgG antibody (BioLegend #410902) was added to each well ...
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bioRxiv - Cancer Biology 2023Quote: ... The primary antibodies that were used in this study included rabbit anti- human CD271 (1:100, HPA004765, Biolegend), mice anti-human CXCL12 (1:50 ...
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bioRxiv - Immunology 2023Quote: Cytokine analysis from co-culture supernatants was performed with Human CD8/NK Panel (13-plex) Cat#741065 (BioLegend) following the manufacturer’s protocol.
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bioRxiv - Immunology 2023Quote: ... Dynabeads M270-Epoxy (Thermo) was covalently conjugated with combinations of mouse anti-human CD3 antibody (clone UCHT1, BioLegend), mouse anti-human CD28 antibody (BioLegend) ...
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bioRxiv - Immunology 2023Quote: ... and 1:20 dilution mouse anti-human CD27-PE (stock 50 ug/mL, BioLegend, San Diego, CA, USA), which has been reported as a marker for juvenile B cells ...
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bioRxiv - Immunology 2023Quote: ... P008_108)52 for 1h at 4°C followed by PE-rat anti-human IgG Fc (1:200, Biolegend) for 45 min at 4°C ...
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bioRxiv - Immunology 2023Quote: ... sorted TN and TEMRA cells were stimulated with plate-bound anti-human CD3 (OKT3; 1 μg/ml; BioLegend) and anti-human CD28 (CD28.2 ...
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bioRxiv - Immunology 2023Quote: ... slides were incubated with primary antibodies to human phospho-6SRP (Ser235/236) (CellSignaling 4858) or CD45 (BioLegend 304002) at 1:50 dilution overnight at 4°C ...
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bioRxiv - Cancer Biology 2023Quote: ... cells (1x106) were stained with PE-labelled anti-human CD38 monoclonal antibody (clone HB-7, BioLegend Cat # 356604) in FACS buffer (0.5% FBS with 2 mM EDTA ...
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bioRxiv - Bioengineering 2024Quote: ... TCR was detected using an anti-human TCR α/β antibody conjugate to BV421 (Biolegend, San Diego, CA). Flow cytometry to assess CAR and TCR positivity was performed on Day 8 of manufacturing on an Attune NxT flow cytometer (ThermoFisher ...
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bioRxiv - Immunology 2024Quote: ... cells were pretreated with an FcR blocker (Miltenyi Biotechnology, #130-092-575 for mouse; BioLegend, #422302 for human) for 10 minutes ...
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bioRxiv - Immunology 2024Quote: ... 5 μl PE-labeled mouse anti-human CD11b fluorescent antibody (cat. no. 301306; 1:1,000 dilution; BioLegend, Inc.) or FITC-labeled mouse anti human CD14 fluorescent antibody (cat ...
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bioRxiv - Neuroscience 2020Quote: ... mouse anti-dephosphorylated neurofilament protein (clone smi32; Biolegend, San Diego, CA, USA); rabbit anti-Caspr1 (clone EPR7828 ...
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bioRxiv - Immunology 2022Quote: ... −phycoerythrin (PE) and −peridinin chlorophyll protein complex (PerCP) were purchased from Biolegend. Phospho-specific mAbs against p65 (S536 ...
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bioRxiv - Microbiology 2020Quote: ... antibodies against the following proteins and epitopes were used: HA (16B12, BioLegend), β-actin (AC-74 ...
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bioRxiv - Immunology 2022Quote: ... Biotinylated protein was then mixed with streptavidin fluorophores (AF647, BioLegend; BV421, BioLegend), as described previously6 ...
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bioRxiv - Immunology 2022Quote: ... Biotinylated protein was then mixed with streptavidin fluorophores (AF647, BioLegend; BV421, BioLegend), as described previously6 ...
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bioRxiv - Bioengineering 2024Quote: The scFc protein was labeled with SNAP-Surface 488 (BG-488, Biolegend). In brief ...