Labshake search
Citations for BioLegend :
5151 - 5200 of 5512 citations for Recombinant Human APCS Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Genomics 2023Quote: ... the cells were resuspended in 100 μl chilled DPBS with 0.2% BSA and 10 μl of human TrueStain FcX (BioLegend, 422302) and incubated on ice for 10 min to reduce non-specific labeling ...
-
bioRxiv - Genomics 2023Quote: ... and all the samples were treated with sybr green (1x) and mouse anti-human IgG conjugated with Alexflour 647 (1:100 dilution, from BioLegend) for 30 mins at room temperature ...
-
bioRxiv - Immunology 2023Quote: ... cells were first resuspended in cell staining media (CSM) (Fluidigm, South San Francisco, CA, USA) with 5ml Human TruStain FcXTM block (Biolegend) for 5 min at room temperature to block Fc receptors ...
-
bioRxiv - Immunology 2023Quote: ... were resuspended in labeling buffer (1% BSA in PBS) and underwent Fc blocking for 10 minutes on ice using Human TruStain FcX (BioLegend). Next ...
-
bioRxiv - Immunology 2023Quote: ... then Fc gamma receptors were blocked for 15 minutes with heat-inactivated human serum prepared in-house or anti-mouse CD16/32 (TruStain FcX, Biolegend) for human samples and mouse samples respectively ...
-
bioRxiv - Cancer Biology 2023Quote: ... Antibodies used to distinguish the different cell populations included Brilliant violet 421 anti-human CD19 (Biolegend, San Diego, CA, USA), PE anti-human CD45 (Biolegend) ...
-
bioRxiv - Microbiology 2023Quote: ... 100,000 cells were plated in 96-well round bottom plate and blocked with 1:1000 Human TruStain FcX™ (Biolegend) diluted in washing buffer for 10 minutes ...
-
bioRxiv - Cancer Biology 2023Quote: ... 100 000-200 000 cells per sample were processed by blocking with 5 µl of Human TruStain FcX Blocking Solution (BioLegend) for 10 min at 4°C ...
-
bioRxiv - Biochemistry 2024Quote: ... following an overnight incubation at 4°C with the primary antibody against residues 103-108 of human α-synuclein (BioLegend, 807801 [4B12] 1:1000 ...
-
bioRxiv - Systems Biology 2024Quote: ... Ultra-LEAF anti-human HLA-A,B,C antibody (clone W6/32, cat#: 311448) for immunopeptidomics was purchased from BioLegend. Polyethylenimine (PEI ...
-
bioRxiv - Immunology 2023Quote: ... the following antibodies were used at a 1:200 ratio: epithelial cells (324216, Biotin anti-human Epcam, clone:9C4, BioLegend), endothelial cells (13-0319-82 ...
-
bioRxiv - Immunology 2023Quote: ... B cells were gated for using 1:20 dilution commercial mouse anti-human CD19-Alexa488 (BioLegend, San Diego, CA, USA) and 1:20 dilution mouse anti-human CD27-PE (stock 50 ug/mL ...
-
bioRxiv - Bioengineering 2023Quote: ... Cells were washed once with PBS and incubated in 50 µL PBS containing human Fc block (Supplementary Table 1) and Zombie Aqua viability dye (1:200, BioLegend) for 15 min at 4°C ...
-
bioRxiv - Cancer Biology 2023Quote: ... Cells were resuspended in FACS buffer (PBS plus 2% FBS) and blocked with monoclonal antibody to CD16/32 (Human TruStain FcX, #422302, Biolegend) for 10 min at 4°C before staining with surface antibodies ...
-
bioRxiv - Cancer Biology 2023Quote: ... and cell surface proteins were stained with the following antibodies (5 μl per test in 100 μl flow buffer) at 4 °C for 20 minutes: FITC anti-human CD45 (368508; BioLegend), FITC anti-human CD14 (325604 ...
-
bioRxiv - Immunology 2023Quote: ... were derived from a cell sort that included an additional cell hashing step (TotalSeq-C0251/C0252/C0253/C0254/C0255 anti-human Hashtag 1/2/3/4/5 antibodies, cat # 394661/394663/394665/394667/394669, Biolegend) prior to B cell enrichment and staining ...
-
bioRxiv - Genomics 2023Quote: ... A subset of the hiPSC lines were evaluated by flow cytometry for expression of two pluripotent markers: Tra-1-81 (Alexa Fluor 488 anti-human, Biolegend) and SSEA-4 (PE anti-human ...
-
bioRxiv - Genomics 2023Quote: ... Many of the iPSC lines were evaluated by flow cytometry for expression of two pluripotent markers: Tra-1-81 (Alexa Fluor 488 anti-human, Biolegend) and SSEA-4 (PE anti-human ...
-
bioRxiv - Genomics 2024Quote: ... A subset of the iPSC lines was evaluated by flow cytometry for expression of two pluripotent markers: Tra-1-81 (Alexa Fluor 488 anti-human, Biolegend) and SSEA-4 (PE anti-human ...
-
bioRxiv - Molecular Biology 2023Quote: ... The percentage of purified CD34+ cell was analysed by flow cytometry staining with anti-human CD34 PE antibody (BioLegend, UK). The cells were cultured in StemSpan SFEM II medium (StemCell Technologies ...
-
bioRxiv - Immunology 2024Quote: ... and 0.75 ug of GAD and Empty tetramers in addition to 1:200 anti-human CD19-BV421 (BioLegend, Cat#363017) and 1:100 anti-human CD3-FITC (BioLegend ...
-
bioRxiv - Immunology 2024Quote: ... cells were incubated for 25 minutes at 4°C with the following antibodies: BV711 anti-mouse/human CD11b (Cat# 101242; BioLegend), PE anti-mouse CX3CR1 (Cat# FAB5825P ...
-
bioRxiv - Immunology 2024Quote: ... It exhibits monocyte morphology and was originally derived from malignant cells obtained from the pleural effusion from a patient with histiocytic lymphoma.77 LFA-1 (CD11a/CD18) was assessed with a FITC anti-human antibody (clone m24, Biolegend), and MAC-1 (CD11b/CD18 ...
-
bioRxiv - Cancer Biology 2024Quote: ... and incubated for 30 min at 4°C with 1 µL of PE anti-human FasL antibody (clone NOK-1) from BioLegend. Nonspecific background signals were measured by incubating cells with the appropriate isotype-matched antibody ...
-
bioRxiv - Cell Biology 2024Quote: ... FBM and ABM cells were resuspended in ice-cold PBS with 5ul of Fc receptor blocker (Human TruStain FcX, Biolegend) and were incubated for 10 min at 4°C ...
-
bioRxiv - Bioengineering 2024Quote: The following anti-human antibodies were used in the Jurkat staining panel: anti-CD4 FITC (BioLegend catalog #317408, clone OKT4), anti-CD25 PerCP/Cyanine (Cy ...
-
bioRxiv - Cell Biology 2024Quote: ... IL-8 concentration in medium samples was measured by using the ELISA MAX Deluxe Set Human IL-8 (BioLegend, 431504) according to the user manual.
-
bioRxiv - Bioengineering 2024Quote: ... Cells were then stained with Brilliant Violet (BV) 421TM anti-human CD138 (Syndecan-1) antibody (BioLegend catalog #356516, clone MI15), FITC anti-human HLA-A ...
-
bioRxiv - Immunology 2024Quote: ... and 5 µg/mL of soluble Ultra-LEAF™ purified anti-human CD28 monoclonal antibody (clone 28.2, BioLegend, cat. 302933) in R10 medium [RPMI-1640 medium supplemented with 10% heat-inactivated FBS ...
-
bioRxiv - Microbiology 2024Quote: ... The cells were then incubated 0.1 % BSA in PBS with or without 1/40 dilution of PE/Cyanine7-conjugated anti-human IL6 (Biolegend, #501119) or 1/80 dilution of PE-conjugated anti-human IL8 (Biolegend ...
-
bioRxiv - Cell Biology 2022Quote: ... mouse monoclonal anti-NPC proteins (FXFG repeat-containing nucleoporins) (1:1,000; mAb414; 902907, Biolegend), rabbit monoclonal anti-BANF1/BAF (1:200 ...
-
bioRxiv - Immunology 2022Quote: ... Briefly PBMC or IDCs were cultured in presence of a protein transport inhibitor (Biolegend) in media alone (control ...
-
bioRxiv - Cancer Biology 2019Quote: ... Biotinylated proteins were detected either by Alexa Fluor®594 Streptavidin (405240, Biolegend®) or Streptavidin-HRP (21130 ...
-
bioRxiv - Immunology 2022Quote: ... Cells were than stained with Siglec-5 Fc chimeric protein (Biolegend, San Diego, CA) pre-incubated with anti-human IgG Fc fluorescent antibody (Biolegend ...
-
bioRxiv - Immunology 2022Quote: ... Antibodies with specificity for the following intracellular and intranuclear proteins were purchased from Biolegend with different fluorochrome labels ...
-
bioRxiv - Genomics 2019Quote: ... or CC2C6 (for pyro-Glu modified protein; BioLegend, Cat. #323123/4, RRID: AB_2716202/3) anti-CD47 antibodies were used with Protein-G DynabeadsTM or Biotin-binder DynabeadsTM ...
-
bioRxiv - Immunology 2021Quote: ... and mouse anti-β-tubulin (1:1000; BioLegend; used as a control loading protein). Membranes were then incubated with appropriate secondary antibody (1:5000 ...
-
bioRxiv - Immunology 2021Quote: ... CD28-peridinin chlorophyll protein (PerCP) Cy5.5 (clone CD28.2; Cat#302922, BioLegend, San Diego, CA), CD95-fluorescein isothiocyanate (FITC ...
-
bioRxiv - Cell Biology 2020Quote: ... Anti-fibroblast specific protein (FSP1, displayed as S100A4; Cat: 370003) was purchased from Biolegend, CA ...
-
bioRxiv - Immunology 2020Quote: ... Antibodies for stimulations and flow cytometry against the following proteins were obtained from BioLegend, eBioscience or BD Pharmingen (clone names indicated in parentheses) ...
-
bioRxiv - Microbiology 2022Quote: ... Serum IL-17A was measured as part of a LEGENDPlex Multiplex protein assay (Biolegend) following the manufacturer’s protocol.
-
bioRxiv - Molecular Biology 2022Quote: ... and probed with protein-specific antisera against RpoB (1:10,000 dilution, BioLegend, cat# 663905), 3xFLAG (1:1,000 dilution ...
-
bioRxiv - Immunology 2024Quote: ... Purified mouse anti-SARS-CoV-2 spike protein S2 antibody (1:3000 dilution, BioLegend) and HRP-conjugated goat anti-mouse secondary antibody (1:3750 dilution ...
-
DTX3L and USP28 fine-tune DNA double strand repair through mutual regulation of their protein levelsbioRxiv - Biochemistry 2023Quote: ... Proteins were detected with monoclonal antibodies against ubiquitin (BioLegend Cat. No. 646304; 1:1000), human HIF-1α (#610959 ...
-
bioRxiv - Immunology 2023Quote: ... biotinylated SARS-CoV-2 Spike RBD protein was mixed with Streptavidin-BV421 (405225, BioLegend) at a 4:1 molar ratio for 1 hour at 4 °C to obtain the antigen probe ...
-
bioRxiv - Biochemistry 2024Quote: ... Pre-stained molecular weight ladders (Prime-Step™ Prestained Broad Range Protein Ladder; BioLegend) were included in all runs to allow size determination of detected bands ...
-
bioRxiv - Biophysics 2021Quote: ... This step was performed in order to break the existing cadherin junctions and allow the blocking antibodies (anti N-Cadherin antibody: LEAF™ Purified anti-human CD325, #350804, Biolegend, USA ...
-
bioRxiv - Cell Biology 2021Quote: ... Then 1×106 cells were washed again with 1x Hanks buffer and stained with 5µl of Alexa Fluor® 647 anti-mouse/human CD15 (SSEA-1) Antibody (Biolegend#125608) in100µl BD Pharmingen™ Stain Buffer (FBS ...
-
bioRxiv - Immunology 2020Quote: ... Blood CD4+ T cells were isolated from healthy donor or patient PBMCs using a human CD4+ T cell isolation kit (BioLegend #480010). Purified CD4+ T cells were used without stimulation ...
-
bioRxiv - Molecular Biology 2021Quote: ... cells were first blocked with 5% BSA for 30 min and stained with Alexa Fluor® 647 anti-human CD161 antibody (clone HP-3G10; BioLegend) at 10 μg/ml in blocking solution for 60 min ...