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Citations for BioLegend :
4801 - 4850 of 5512 citations for Recombinant Human APCS Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2020Quote: ... frozen PBMC were thawed and stained with 2 μl of anti-human CD3-AF488 (clone UCHT1, Biolegend) and 1 μl of anti-human CD14-PE (clone 61D3 ...
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bioRxiv - Immunology 2020Quote: ... Cells were subsequently stained with Brilliant Violet 785™ anti-human CD14 Antibody (1:1000; BioLegend, #301840), APC anti-human CD86 Antibody (1:1000 ...
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bioRxiv - Immunology 2022Quote: ... IL-2 concentration in harvested supernatant was then analyzed using Human IL-2 ELISA Max Kit (BioLegend) per provided instruction ...
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bioRxiv - Immunology 2020Quote: ... fluorochrome-conjugated antibodies (anti-mouse CD45-BioLegend Cat. No. 103126, anti-human CD45-BioLegend Cat. No. 304014), fixed with formalin ...
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bioRxiv - Immunology 2021Quote: ... Antibodies used to stain transfected 293T cells were Brilliant Violet 421-conjugated anti-human CD3 antibody (Biolegend) and phycoerythrin (PE)-conjugated anti-human TCRαβ antibody (BD BioSciences).
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bioRxiv - Immunology 2020Quote: ... We followed manufacturer instructions for the LEGENDplex™ Human Th Panel (13-plex) kit (BioLegend, Cat #740722) in a 96-well V bottom plate ...
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bioRxiv - Cancer Biology 2019Quote: The cells were stained with anti-human monoclonal antibody (mAb) CD4–FITC clone RPA-T4 (BioLegend, CA). For nuclear staining ...
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bioRxiv - Cell Biology 2021Quote: ... were blocked with 5ul Human TruStain FcX™ Blocking Reagent for 10 minutes at 4°C (BioLegend). 1ug of each TotalSeq™-A antibody was added to the cell suspension ...
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bioRxiv - Cell Biology 2019Quote: ... EPO level was measured in the conditioned media using the LEGEND MAX Human Erythropoietin ELISA kit (Biolegend), according to manufacturer’s instructions.
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bioRxiv - Developmental Biology 2021Quote: ... PSC-derived endothelial cells were sorted by gating for CD31+ (PE anti-human CD31, Biolegend, 1:200) cells.
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bioRxiv - Microbiology 2021Quote: ... CD4+ T cells were isolated by negative selection using MojoSort Human CD4 T Cell Isolation kit (Biolegend). Primary CD4+ T cells were maintained in complete RPMI 1640 medium supplemented with 10 IU/ml IL-2 (Center for AIDS Reagents [CFAR] ...
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bioRxiv - Microbiology 2020Quote: ... cells were stained for 30 min at 4°C with FITC-conjugated anti-human CD3 antibody (Biolegend), followed by fixation and permeabilization of cells with BD Cytofix/Cytoperm™ (BD Bioscience) ...
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bioRxiv - Immunology 2021Quote: ... CD4+ cells were isolated by negative-selection using MOJOSORT™ Human CD4 T Cell Isolation Kit (Biolegend) and a EASYSEP™ Magnet (StemCell ...
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bioRxiv - Immunology 2021Quote: ... PE anti-human HLA-A,B,C (clone W6/32; dilution 1:30; Biolegend; Cat no 311406), PerCP/Cyanine5.5 anti-human CD3 (clone SK7 ...
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bioRxiv - Cancer Biology 2022Quote: ... T cells were separated from PBMCs using a MojoSort human CD3+ T cell isolation kit (Biolegend, USA).
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bioRxiv - Immunology 2022Quote: ... and Alexa Fluor 647-anti mouse/human CD324 (E-cadherin) (clone DECAMA-1) was obtained from Biolegend.
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bioRxiv - Immunology 2022Quote: ... Human transfected HLA-A2.1+ CD8+ T cells were stained with: anti-mouse TCR-β-FITC (Biolegend, 109205), anti-mouse TCR-β-BV421 (Biolegend ...
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bioRxiv - Immunology 2022Quote: ... in the presence or absence of a mouse anti-human PD1 (2 μg/mL) blocking antibody (Biolegend). CD4+ T-cells were identified using a mouse-anti human CD4 (OKT4 clone ...
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bioRxiv - Neuroscience 2022Quote: ... Recovered cells were stained anti-mouse/human CD11b antibody clone M1/70 (BV605; Biolegend, San Diego, CA), washed ...
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bioRxiv - Cell Biology 2022Quote: ... cells were incubated with Alexa Fluor 488 anti-mouse/human Mac-2 (Galectin-3) antibody (125410, Biolegend) or alternatively with Brilliant Violet 421 anti-mouse/human Galectin-3 antibody (125416 ...
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bioRxiv - Immunology 2024Quote: ... Donor 5 and 6 PBMCs were stained with a cocktail of anti-human CD3 PerCP-Cy5.5 (Biolegend), CD8 PerCP-Cy5.5 (Biolegend) ...
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bioRxiv - Cancer Biology 2024Quote: ... T cells were isolated using the MojoSort Human CD3 T Cell Isolation Kit (cat. no. 480021, Biolegend) and EasySep Magnet (cat ...
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bioRxiv - Cancer Biology 2024Quote: ... Alexa-fluor 488 anti-mouse CD49b (Clone HMα2; 103510) and mouse anti-human LAMP2 (354303) from BioLegend, primary antibodies for mouse anti-human CD49b (611017 ...
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bioRxiv - Immunology 2024Quote: ... anti-human CD45 (clones T29/33 and BC8 from Leinco Technologies; clones 2D1 and HI30 from BioLegend).
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bioRxiv - Immunology 2024Quote: ... in complete medium supplemented with anti-human CD28 antibody (5 µg/mL) (BioLegend, San Diego, CA, US), rhIL2 (50 U/mL ...
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bioRxiv - Immunology 2024Quote: ... T cell activation was assessed by incubating cells with fluorescein isothiocyanate-conjugated anti-human CD8 (Biolegend, 100705) and phycoerythrin-conjugated anti-human CD25 (Biolegend ...
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bioRxiv - Immunology 2024Quote: ... 1 mL 50 mg/mL PE/Dazzle594-mouse-anti-human CD16 (BioLegend; Cat. No. 302054; clone 3G8), 1 mL 100 mg/mL PE/Cy7-mouse-anti-human CD39 (BioLegend ...
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bioRxiv - Immunology 2024Quote: ... 1 mL 100 mg/mL PE/Cy7-mouse-anti-human CD39 (BioLegend; Cat. No. 328212; clone A1), 1 mL 50 mg/mL APC-mouse-anti-human CD56 (BioLegend ...
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bioRxiv - Cell Biology 2023Quote: ... U266B1 and IM-9 cells were evaluated directly with PE-conjugated anti-human DR4 antibody (B376455, Biolegend) and PE-conjugated anti-human DR5 antibody (B347680 ...
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bioRxiv - Immunology 2023Quote: ... supernatants from cell cultures were harvested for detecting cytokine using LEGENDplexTM Human Th1 Panel (5-Plex) (BioLegend). Samples were diluted 5-fold using assay buffer and then mixed with beads and shaken for 2 hours at 500 rpm in a 96 plate well ...
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bioRxiv - Immunology 2023Quote: ... stable transfectants were screened by flow cytometry using mouse anti human PD1 (cat: 621610, BioLegend, CA, USA) and isotype control using allophycocyanin (APC)-conjugated mouse IgG1(cat ...
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bioRxiv - Immunology 2023Quote: Engineered T-cells were stained with increasing concentrations of AF647-conjugated anti-human αβTCR antibody (IP26: Biolegend) and HLA.A2/9V-AF647 for determination of label saturation concentration of CMV-specific RA14 TCR T-cells and HLA.A2/NY-ESO-1-specific engineered T-cells ...
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bioRxiv - Immunology 2023Quote: ... Target cell lines were stained using phycoerythrin (PE)-conjugated mouse anti-human PDL1 antibody (cat: 329706, BioLegend) and APC-conjugated mouse anti-human PDL2 antibody (cat ...
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bioRxiv - Cancer Biology 2023Quote: ... The pellet was resuspended in red blood cell lysis buffer (BioLegend, catalog no. 420301, all human samples) and incubated for 3 min on ice with periodic shaking ...
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bioRxiv - Immunology 2023Quote: ... ELISA for IFN-γ and IL-10 were also performed with human ELISA MAX Deluxe Sets (BioLegend) to verify the multiplex ELISA results ...
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bioRxiv - Cancer Biology 2023Quote: ... Fc-block was performed by incubating cells in a dilution of Human TruStain FcX (#422302, BioLegend, USA) and mouse TruStain FcX (#101320 ...
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bioRxiv - Genetics 2023Quote: ... splenic B cells were collected and stained with FITC anti-mouse/human GL7 (Biolegend, Cat. No. 144604) and PE hamster anti-mouse CD95 (BD ...
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bioRxiv - Microbiology 2023Quote: ... for 30 minutes at RT after adding Human TruStain FcX (1:200 dilution, Biolegend, San Diego, CA) for 5 minutes ...
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bioRxiv - Cancer Biology 2023Quote: ... and Brilliant Violet 510™ anti-human CD45 (clone HI30, dilution 1/20, Biolegend, Amsterdam, The Netherlands) antibodies were used for progenitor cell subset phenotyping ...
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bioRxiv - Immunology 2023Quote: ... Commercial anti-RBD antibodies (mouse IgG1κ, clone 1035753, R&D systems or human IgG1κ, clone AM001414, BioLegend) and isotype controls (mouse IgG1κ ...
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bioRxiv - Neuroscience 2023Quote: ... Concentrations of chemokines in cell supernatants were measured using the LEGENDplexTM Human Proinflammatory Chemokine Panel 1 (Biolegend). Readings were made on an AttuneTM Nxt Flow Cytometer.
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bioRxiv - Microbiology 2023Quote: ... Cells were incubated with 2 μg/mL of phycoerythrin (PE) anti-human IgG Fc (Clone M1310G05, BioLegend). Cells were washed once ...
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bioRxiv - Genomics 2023Quote: ... The PBMC pellet was resuspended and cells were stained with Human TruStain FcX Fc Blocking Reagent (BioLegend) for 10 min at 4°C and then with fixable viability dye DAPI (Merck) ...
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bioRxiv - Cancer Biology 2023Quote: TCR-transduced cell lines were stained with anti-human TCR α/β (Clone IP26, BioLegend Cat. #306717) and anti-human CD3 antibody (Clone HIT3a ...
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bioRxiv - Immunology 2023Quote: ... we used Ultra-LEAF™ purified anti-human TIM-3 antibody (10μg/ml, clone F38-2E2, Biolegend) or its respective isotype Ultra-LEAF™ purified mouse IgG1 antibody (10μg/ml ...
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bioRxiv - Cancer Biology 2024Quote: ... Samples were randomized on the assay plates and analyzed using the LEGENDplex human CD8/NK panel (BioLegend). Samples were read on a CytoFLEX S flow cytometer (Beckman Coulter) ...
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bioRxiv - Neuroscience 2024Quote: ... the iAstrocytes were stained with 1:50 PE anti-human HLA-A,B,C Antibody (BioLegend, 311406) and DAPI for 30 minutes at 4°C ...
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bioRxiv - Cancer Biology 2024Quote: ... or Anti-human/mouse CD49f antibody (#313620, Biolegend) (isotype control, Pacific Blue Rat IgG2a, k; #400527, Biolegend). For GD3 FACS analysis ...
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bioRxiv - Genomics 2024Quote: ... for 10 minutes at 4 °C and then stained with FITC - anti-human EPCAM (BioLegend CAT #32404) and PE - anti-CD45 (BioLegend ...
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bioRxiv - Cancer Biology 2024Quote: ... a single-cell solution of lung cells was incubated with Human TruStain FcX (Biolegend, #422302, 1:20) for 10 minutes ...