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Citations for BioLegend :
4651 - 4700 of 8552 citations for 5 Pyrimidinecarbonitrile 1 2R 2 3 dihydroxypropyl 1 2 3 4 tetrahydro 3 methyl 2 4 dioxo 9CI since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2021Quote: ... 0.1% BSA) before secondary detection with 1:100 dilutions of APC- conjugated mouse anti-hemagglutinin tag (HA).11 antibody (BioLegend, 901524), PE-conjugated goat anti-human IgG polyclonal antibodies (Southern Biotech ...
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bioRxiv - Cancer Biology 2020Quote: ... Cells were blocked using DMEM/FBS media for 1 hour at RT followed by incubation with 1:1000 dilution mouse anti-HA (Biolegend, 901514) and rabbit anti-PCNA (Cell Signaling ...
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bioRxiv - Immunology 2022Quote: CD4+ T cells (1 × 106) were stimulated for 7 days at 37°C with anti-mouse CD3 (BioLegend; 1 μg/mL) and CD28 (BioLegend ...
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bioRxiv - Neuroscience 2022Quote: ... Primary immunoreaction solution consisted of blocking buffer that contained 1:1000 rabbit anti-melanopsin (ATS Bio AB N38) and 1:250 mouse anti-SMI32 (Biolegend 801701). After incubation in the primary immunoreaction solution ...
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bioRxiv - Immunology 2022Quote: ... Cells were then stained with rat polyclonal T antigen antibody (1:500) followed by an anti-rat secondary (Biolegend, 1:200). For measuring sialic acid binding dependence ...
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bioRxiv - Genomics 2022Quote: ... Cells were then resuspended in 120 μl FACS buffer with live/dead staining was performed using 1 μg ml−1 propidium iodide (BioLegend, 421301) and kept on ice until sorting ...
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bioRxiv - Molecular Biology 2021Quote: ... for 1 hour following disaggregation before being stained (anti-CD71 Brilliant Violet 421, 1:5000 dilution – 113813 Biolegend, RRID: AB_10899739; anti-Ter119 Alexa Fluor 647, 1:1000 dilution – 116218 Biolegend RRID: AB_528961) in FACS buffer and sorted based on CD71 and Ter119 signal (Fig ...
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bioRxiv - Systems Biology 2022Quote: ... membranes were blocked with 5% non-fat milk at room temperature (RT) for 1 h and probed with primary RpoC (β prime subunit of RNAP) antibodies at 1:2000 dilutions (Biolegend) at 4 °C overnight ...
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bioRxiv - Immunology 2021Quote: ... were co-cultured with 1×105 relevant target cells (10:1 E:T ratio) overnight in the presence of Brefeldin A (BFA) (Biolegend, CA, USA), and the next day ...
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bioRxiv - Cell Biology 2022Quote: ... The remaining cells were incubated with Biotin anti-mouse Ly-6A/E (Sca-1) antibody (Biolegend; Cat. No. 122504; 1:75) at 4°C for 20 minutes then 10 µl Streptavidin beads (1:30 ...
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bioRxiv - Genomics 2022Quote: ... Cell pellets were resuspended in FACS buffer (PBS with 1% FBS and 1% penicillin-streptomycin) then stained with the following antibodies: PE anti-CD45 (BioLegend #103105); APC anti-CD31 (Invitrogen #17-0311-80) ...
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bioRxiv - Immunology 2024Quote: The following antibodies were used: unconjugated anti-CD16/CD32 (1 μg per 1×106 cells; clone 93; BioLegend Cat. No. 101320), H2-Dd-FITC (1:200 ...
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bioRxiv - Immunology 2024Quote: Murine DCs were differentiated from isolated bone marrow via culture in RPMI + 10% FCS 1% Pen/Strep and 1% Amino Acids containing 20 ng/ml GMCSF (Biolegend, #576306) on silicone hydrogels with different stiffnesses ...
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bioRxiv - Microbiology 2023Quote: ... and 20 minute incubation with primary antibody cocktail (CD45+ immune cells: 1:50 anti-CD45-VioGreen [Miltenyi Biotec, Germany]; CD31+ endothelial cells: 1:2000 anti-CD31-PE [BioLegend, CA]/1:50 anti-CD45-VioGreen [Miltenyi Biotec ...
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Evolution of protease activation and specificity via alpha-2-macroglobulin-mediated covalent capturebioRxiv - Synthetic Biology 2023Quote: ... was first applied for 1 h followed by staining with PE anti-streptavidin (1:200, Biolegend, cat. no. 410503, clone 3A20.2) and anti-c-myc-FITC (1:50 ...
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bioRxiv - Molecular Biology 2023Quote: ... and APC/Fire 750 conjugated anti E-Cadherin (dilution: 1:100; clone DECMA-1. All antibodies purchase from BioLegend (CA, USA). Samples were washed in 1mL 2% FBS and PBS and centrifuged at 2000rpm for 5mins ...
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bioRxiv - Immunology 2023Quote: ... Stained cells and “fluorescence minus one” (FMO) controls were then resuspended in SB + 1:1000 DNase + 1:1000 Draq7 (BioLegend, #424001) as a live/dead stain ...
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bioRxiv - Immunology 2023Quote: ... Alexa Fluor 488 or PE–conjugated rat anti-mouse CD31 [platelet endothelial cell adhesion molecule 1 (PECAM1)] antibody (1:200; BioLegend, MEC13.3), Alexa Fluor 647 or FITC–conjugated rat anti-mouse CD326 (Ep-CAM ...
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bioRxiv - Immunology 2023Quote: ... PE-anti-PD-1 (clone RMP1-30, 1:20) and its isotype control PE-Rat IgG2b,κ (clone RTK4530, 1:20) were purchased from Biolegend. PE-anti-PD-L1 (clone MIH5 ...
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bioRxiv - Immunology 2023Quote: ... The reaction was stopped by centrifugation at 4°C followed by staining of cell surface LAMP-1 (CD107a) using FITC-coupled anti-LAMP-1 antibody (clone 1D4B, BioLegend, #121605) for 20 minutes at 4°C in the dark ...
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bioRxiv - Immunology 2023Quote: ... resuspended in 1 ml of PBS containing 1 µl live/dead stain (Zombie UV Fixable Viability kit (Biolegend, cat. no. 423107)) and incubated on ice for 30 min ...
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bioRxiv - Neuroscience 2023Quote: ... CD3-APC-Cy5.5 (1:40, Abcore #AC12-0212-04), CD4-PerCP-eFluor710 (1:40, eBioscience #46-0040-82) and CD8a-PE (1:40, BioLegend #200607) antibodies in FACS buffer with 2 µg DAPI to distinguish cells from debris ...
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bioRxiv - Neuroscience 2024Quote: ... PE-conjugated anti-IL-17 (1:30, eBioscience Cat# 12-7177-81, RRID:AB_763582) and PE-CF594- conjugated anti-FoxP3 (1:50, Biolegend Cat# 320008, RRID:AB_492980) in Cytoperm at room temperature for 30 min ...
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bioRxiv - Neuroscience 2023Quote: ... brains were incubated with 1:1000 chicken anti-GFP antibody (Aves GFP-1020) and 1:1000 mouse anti-tyrosine hydroxylase antibody (BioLegend 818001), with boosters over 5 days for a final concentration of 1:200 ...
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bioRxiv - Neuroscience 2023Quote: ... Primary antibodies to the following proteins were used at the indicated concentrations: Ubq (1:5000, P4D1, Cell Signaling; 1:5000, P4G7-HRP, BioLegend), Actin (1:10,000 ...
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bioRxiv - Neuroscience 2024Quote: ... 1% normal donkey serum and 0.3% Triton X-100) and incubated in primary antibodies against FOX3 (NeuN, BioLegend, 834501, 1:1000), OLIG2 (R&D ...
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bioRxiv - Neuroscience 2024Quote: ... CD3-APC-Cy5.5 (1:40, Abcore #AC12-0212-04), CD4-PerCP-eFluor710 (1:40, eBioscience #46-0040-82) and CD8a-PE (1:40, BioLegend #200607) antibodies in FACS buffer with 2 µg of DAPI to distinguish cells from debris for 1 h at 4°C ...
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bioRxiv - Molecular Biology 2024Quote: ... and resuspended in a cocktail of FACS buffer with anti-FCR (made in house; 1:10) and fluorochrome-conjugated antibodies against B220 (RA3-6B2-BV605; 1:200; BioLegend #103244), TCRβ (H57-597-PE-Cy7 ...
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bioRxiv - Cancer Biology 2021Quote: ... tissues were incubated overnight at room temperature with 1 μg.ml−1 DAPI or 0.5 μM Sytox™ Blue solution (Biolegend, catalog no. 425305) to counterstain cell nuclei and mounted using Vectashield mounting media (Vector Laboratories ...
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bioRxiv - Cell Biology 2020Quote: ... The following FACS antibodies were used at 1:200: mouse anti-human CD45 APC-Cy7 (BioLegend; Cat#304014; used at 1:200), mouse anti-human anti-CD11b-APC-Cy7 (BD ...
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bioRxiv - Immunology 2020Quote: ... CAFs were co-cultured overnight with CD4+ T cells at a 1:1 ratio in the presence of pan-HLA antibody (5μg/ml, Biolegend, Cat. No.361702) or GC1q R antibody (10μg/ml ...
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bioRxiv - Neuroscience 2020Quote: ... The cells were incubated with primary antibodies diluted in 1 % BSA/0.3 % Triton X- 100/DPBS (mouse anti-HA (BioLegend, 901501; 1:1000), mouse anti-myc (NEB/Cell Signaling ...
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bioRxiv - Neuroscience 2021Quote: ... assays were performed using the in situ red starter kit according to the manufacture’s protocol with JADE1 antisera (1:20) and 0N tau antisera (1:500; BioLegend, San Diego, CA). Two control assays were also performed ...
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bioRxiv - Neuroscience 2021Quote: ... The exception is that secondary labeling was accomplished using anti-rat DyLight 405 (1:5000, Novus, NBPI-72983) or anti-mouse DyLight 405 (1:1000, BioLegend, Poly-24091). Fixed cells were then imaged on a Nikon Eclipse TE2000 inverted microscope using a 60x 1.49NA oil-immersion TIRF objective and 405nm and 560nm solid lasers using a CoolSNAP HQ2 CCD camera ...
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bioRxiv - Microbiology 2020Quote: ... Cells were immunostained with the following antibodies: rabbit anti-IFITM3 (Cat. nr. AP1153a, Abcepta, 1:500) and mouse anti-HA (clone 16B12, BioLegend, 1:1400). Secondary antibodies conjugated to Alexa® Fluor 488 or 647 (Invitrogen ...
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bioRxiv - Cancer Biology 2022Quote: ... Livers were sliced into 300μm thick sections on a vibratome and stained with Hoechst (1:2000) and directly conjugated fluorescent antibodies CD31 (Biolegend, 1:100, 390), CD8 (Biolegend ...
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bioRxiv - Physiology 2019Quote: ... overnight at a 1:1 ratio with the indicated target melanoma cells or activated with 1μg/ml plate-bound anti - CD3 (Biolegend, clone: 145-2C11) as indicated in each experiment ...
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bioRxiv - Physiology 2019Quote: ... were co-cultured for 6h at 37°C at a 1:1 ratio with the indicated target melanoma cells or activated with 1μg/ml anti-CD3 (Biolegend, clone: 145-2C11). After 2 h ...
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bioRxiv - Cell Biology 2021Quote: ... The fixed cells were permeabilized with 0.1% Triton-X 100 for 10 min at RT and then incubated with chicken anti-vimentin (1:200, 919101, Biolegend, CA, USA) for 30 min in phosphate buffered saline (PBS ...
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bioRxiv - Microbiology 2022Quote: ... This membrane was blocked with 5% milk and then incubated simultaneously with rabbit polyclonal α-MshA (1:1000, gift of J. Zhu) and mouse monoclonal α-RpoA (1:1000, Biolegend) (as a loading control ...
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bioRxiv - Neuroscience 2022Quote: ... mouse anti-myelin-associated glycoprotein (MAG, 1:100, SigmaAldrich MAB1567),chicken anti-glial fibrillary acidic protein (GFAP, 1:500, BioLegend PCK-591P), chicken anti-GFAP (1:500 ...
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bioRxiv - Pathology 2024Quote: ... amyloid-β (clone 6F/3D, 1:500, Dako, Denmark for DLB donors, and clone 4G8, 1:8000, Biolegend, USA for AD donors) and hyperphosphorylated tau (p-tau ...
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bioRxiv - Pathology 2024Quote: ... then incubated with primary antibody anti-IBA1 (Wako; 011-27991; dilution 1:100) and anti-Amyloid Beta1-42 (Biolegend; 805501; 1:500) prepared in 1% BSA for overnight at 4°C ...
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bioRxiv - Developmental Biology 2024Quote: ... CD5-APC-eF780 (Thermo Fischer Scientific, Catalog No. 47-0015-82, 1/500) and Streptavidin-BV510 (Biolegend, Catalog No. 405234, 1/500). After final washes ...
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bioRxiv - Biochemistry 2023Quote: ... The fixed cells were permeabilized with 0.1% Triton-X 100 for 10 min at RT and then incubated with chicken anti-vimentin (1:200, 919101, Biolegend, CA, USA) for 30 min in phosphate buffered saline (PBS ...
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bioRxiv - Immunology 2023Quote: ... CAR-T cells (1×105 cells/well) and Nalm6-GD2 (1×105 cells/well) were plated in Nalm6-GD2 culture medium containing 1×Brefeldin A (Biolegend, Cat# 420601) for 5-7h ...
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bioRxiv - Microbiology 2020Quote: ... and/or monoclonal anti-RNA polymerase (1:1,2000, Biolegend). Western blots were probed with IRDye-conjugated secondary antibodies and visualized with Odyssey CLx imagining system (Li-COR Biosciences ...
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bioRxiv - Cancer Biology 2021Quote: ... and 1 µg of anti-CD45RO (clone UCHL1, BioLegend) antibodies ...
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bioRxiv - Cancer Biology 2021Quote: ... rabbit anti-Pax6 (1:1000 dilution; Cat. #: 901301, Biolegend); rabbit anti-NeuN (1:1000 dilution ...
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bioRxiv - Cancer Biology 2021Quote: ... and anti-PD-1(CD279)-PE/Cy7 (BioLegend 135216). The myeloid antibody cocktail contained ...