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Citations for BioLegend :
401 - 450 of 552 citations for Dengue Virus Serotype 3 DIII envelope protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2023Quote: ... were derived from a cell sort that included an additional cell hashing step (TotalSeq-C0251/C0252/C0253/C0254/C0255 anti-human Hashtag 1/2/3/4/5 antibodies, cat # 394661/394663/394665/394667/394669, Biolegend) prior to B cell enrichment and staining ...
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bioRxiv - Immunology 2023Quote: ... 106 of the CTV labeled T cells were stimulated in vitro for 3 or 5 days in cultures containing 100ng/ml IL-15 (BioLegend) or a combination of 100ng/ml IL-7 (BioLegend ...
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bioRxiv - Immunology 2019Quote: ... IFN-β1 (cat. no. 581302) and IFN-γ (cat. no. 575302) proteins were purchased from Biolegend.
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bioRxiv - Immunology 2021Quote: ... Bio-PyS23 protein (44.8 kDa) at 16 μM was combined with SA-APC/Fire750 antibody (Biolegend) at 2 µM and was used as a decoy ...
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bioRxiv - Immunology 2020Quote: ... Samples were normalized to 0.5 mg/mL protein in Legendplex assay buffer (Biolegend, San Diego, CA) using a Bradford Assay (BioRad ...
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bioRxiv - Bioengineering 2022Quote: ... were coated with 100ng per well of recombinant Spike RBD protein in 1×coating buffer (Biolegend) overnight at 4C° ...
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bioRxiv - Immunology 2020Quote: ... Proteins were detected by immunoblotting using mouse anti-human CD9 (clone HI9a, BioLegend, dilution 1:1.000), mouse anti-human CD63 (clone TS63 ...
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bioRxiv - Immunology 2022Quote: ... and a human cell surface protein antibody panel containing 277 oligo-conjugated antibodies (TotalSeq-A, BioLegend) including 6 TotalSeq-A isotype controls (table S4) ...
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bioRxiv - Immunology 2023Quote: Antigen-specific B cell subsets were detected using biotinylated SARS-CoV-2 RBD protein (793906; Biolegend) in combination with streptavidin (SAv)-fluorophore conjugates as described [7] ...
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bioRxiv - Cell Biology 2023Quote: ... proteins were labeled with primary antibodies against CD9 (1:1,000; clone HI9, Biolegend, San Diego, USA), CD44 (1:400 ...
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bioRxiv - Neuroscience 2023Quote: ... 1 µl of TotalSeqB Hashtag (anti-Nuclear Pore Complex Proteins Hashtag at 0.5g, Biolegend 682239-682243) was added to each sample for subsequent identification in downstream analysis ...
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bioRxiv - Immunology 2023Quote: ... BA.1 or BA.2 biotinylated RBD protein conjugated with TotalSeq™-C0971 Streptavidin (BioLegend, 405271) and TotalSeq™-C0972 Streptavidin (BioLegend ...
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bioRxiv - Synthetic Biology 2024Quote: ... cell supernatants were collected to monitor IFNG protein expression by ELISA according to manufacturer protocols (BioLegend). Antibodies used ...
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bioRxiv - Developmental Biology 2024Quote: ... labeled with 1 μg of a unique TotalSeq anti-Nuclear Pore Complex Proteins Hashtag Antibody (Biolegend), and then incubated on ice for 30 minutes ...
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bioRxiv - Cancer Biology 2024Quote: ... Quantitative analysis of secreted proteins was performed using a LEGENDplexTM Custom Mouse 14-plex Panel (BioLegend).
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bioRxiv - Cancer Biology 2020Quote: ... VDR overexpressing SKOV-3 and ECC-1 cells were processed and stained with PDL1-BV605 antibody (Biolegend Inc, Catalog number:124321). VDR expressing SKOV-3 and ECC-1 cells ...
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bioRxiv - Immunology 2022Quote: ... Every 10^6 B cells in 100 μL solution were then stained with 3 μL FITC anti-human CD20 antibody (BioLegend, 302304), 3.5 μL Brilliant Violet 421 anti-human CD27 antibody (BioLegend ...
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bioRxiv - Microbiology 2021Quote: ... Tissues were incubated with primary antibodies (mouse anti-HuC/D IgG clone 16A11 at 1:200 [Thermofisher], rabbit polyclonal anti-tubulin β-3 IgG at 1:500 [Biolegend]) in PBS containing 1% Triton X-100 for 48 h at 4 °C ...
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bioRxiv - Immunology 2022Quote: ... Every 106 B cells in 100 μl solution were stained with 3 μl FITC antihuman CD20 antibody (BioLegend, 302304, clone: 2H7), 3.5 μl Brilliant Violet 421 antihuman CD27 antibody (BioLegend ...
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bioRxiv - Molecular Biology 2020Quote: ... Linker oligo with 3’-terminal biotin was purchased from IDT (Coralville, Iowa) and purified streptavidin was purchased from BioLegend (Biolegend, #405150). Biotinylated antibody ...
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bioRxiv - Cell Biology 2021Quote: ... T cells were isolated and stimulated for 48 h with 3 μg/ml plate-bound anti-mouse CD3 antibody (100340, BioLegend, USA) and 2 μg/ml soluble anti-mouse CD28 antibody (102116 ...
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bioRxiv - Biochemistry 2020Quote: ... Cells were blocked with PBS containing 3% BSA and stained with either APC-conjugate anti-human CD99 antibody clone 3B2/TA8 (1:20 dilution, BioLegend 341307), PE anti-human CD166 clone 3A6 (1:20 dilution ...
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bioRxiv - Microbiology 2021Quote: ... Sections were blocked with 10% sheep serum and 1% BSA in TBS for 30 min then incubated at 4°C overnight with 1 μg ml−1 rabbit polyclonal anti-tubulin β-3 IgG (Biolegend) and 1% BSA in TBS ...
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bioRxiv - Cancer Biology 2022Quote: Oligo-labeled antibody staining was done on both melanoblasts and melanom cells using the TotalSeq™-A Antibodies and Cell Hashing with 10× Single Cell 3′ Reagent Kit (Biolegend). The HashTag Oligonucleotidess used for the analysis are mHTO01 ...
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bioRxiv - Cancer Biology 2022Quote: ... spun at 700 g for 3 minutes at 4°C and washed in MACS Buffer 3× before being incubated with the following antibodies: APC-conjugated rat anti-CD3 (5 μL/10×106 cells; Biolegend, 100408), PE-conjugated rat anti-CD8b (2.5 μL/10×106 cells ...
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bioRxiv - Cancer Biology 2023Quote: ... Wells were washed 3 times before adding 50 µL per well of Biotin anti-human IFN-γ Antibody (clone 4S.B3; #502504, BioLegend, USA) diluted 1:400 in 1% SureBlock solution and incubated 1 h at RT ...
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bioRxiv - Genetics 2023Quote: ... Recombinant Anti-AKT1 + AKT2 + AKT3 (phospho S472 + S473 + S474, Purified anti-Tubulin β-3 (TUBB3) Antibody 1:1000 (Biolegend, # PRB-435P), anti-GAPDH antibody 1:1000 (Sigma Aldrich ...
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bioRxiv - Immunology 2023Quote: ... Cells were rinsed with FACS buffer (PBS containing 0.5% BSA and 2mM EDTA) for 3 min at 300g and incubated with Fc Receptor Blocking solution (Human TruStain FcX™, Biolegend) for 10 minutes prior to cell surface staining with fluorescently labeled antibodies in Brilliant Stain buffer (BD Biosciences ...
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bioRxiv - Immunology 2023Quote: ... 1x106 B cells in 100 μl buffer were incubated with a panel of antibodies including 3 μl FITC anti-human CD20 antibody (BioLegend, 302304), 3.5 μl Brilliant Violet 421 anti-human CD27 antibody (BioLegend ...
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bioRxiv - Bioengineering 2024Quote: ... hiPSC-CMs were dissociated by enzymatic digestion (Miltenyi Multi Tissue dissociation kit 3) and stained with the Zombie NIR™ Fixable Viability Kit (BioLegend). Then ...
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bioRxiv - Neuroscience 2022Quote: ... in a protein low-binding microcentrifuge tube separately and incubated with 5 μL Fc blocking reagent (BioLegend) for 10 min at 4°C ...
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bioRxiv - Immunology 2019Quote: ... The following proteins and concentrations were used: ICAM-1-Fc (Cat. 553006, BioLegend, at 10 μg/mL), CD3 (clone 145-2C11 ...
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bioRxiv - Immunology 2019Quote: ... Tissue cytokines in the heart protein extracts were assayed using the mouse Th2 Panel LEGENDplex assay (BioLegend), according to the manufactures instructions.
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bioRxiv - Immunology 2020Quote: ... Supernatants were normalised to protein concentration before being subject to an ELISA protocol or TNF-α (Biolegend), IL-12 (Biolegend ...
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bioRxiv - Immunology 2022Quote: ... and detection of indicated proteins was carried out using the following antibodies: Eos (W15169A; BioLegend, 1:500), pY-STAT5 (BD Biosciences ...
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bioRxiv - Immunology 2022Quote: ... TNF-α and IL-6 protein were quantified using ELISA MAX Deluxe kits (BioLegend, San Diego, CA), according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2023Quote: CD41 and CD62p proteins on platelets were stained with FITC conjugated anti-CD41 (BioLegend 303704,1:50 dilution) and PE conjugated anti-CD62p (BD Pharmingen 561921 ...
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bioRxiv - Immunology 2023Quote: ... Blocking antibodies specific for the following proteins were used: 2.5 μg/ml CD11b (M1/70, BioLegend, #101224), 2.5 μg/ml CD11c (N418 ...
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bioRxiv - Immunology 2023Quote: ... The gels were transferred to nitrocellulose and Mab-associated proteins were detected with anti-C3 (BioLegend, 846302), -C1q (Novus ...
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bioRxiv - Neuroscience 2023Quote: Primary antibodies to the following proteins were used at the indicated concentrations: ubiquitin (1:1000, P4G7, BioLegend; 1:200 Cell Signaling ...
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bioRxiv - Immunology 2023Quote: ... Intracellular proteins were stained using the following antibodies: anti-human T-bet PE (1:20, 4B10, BioLegend), and anti-human Ki-67 BUV395 (1:50 ...
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bioRxiv - Immunology 2023Quote: ... cells were stimulated for the following 5h using a combination of protein transport inhibitor (Monensin) (Biolegend, USA), Ionomycin (1 ug/ml) ...
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bioRxiv - Cancer Biology 2024Quote: Oligonucleotides were conjugated to two antibodies against HGSOC cell surface proteins β2-Microglobulin (β2M) and CD298 (BioLegend) by iEDDA-click chemistry according to CITE-seq antibody-oligo conjugation protocol (https://cite-seq.com) ...
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bioRxiv - Genomics 2019Quote: ... clones that survived after 3-4 months of culture were washed with PBS and stained with FITC anti-HLA-DQ (Biolegend, Clone: HLADQ1) for 30 minutes on ice and cell surface expression assessed by flow cytometry ...
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bioRxiv - Immunology 2021Quote: ... plates were coated at least 5 hours prior to use with 3 ug/ml plate-bound anti-CD3 antibody (clone OKT3; BioLegend, LEAF grade), 1 ug/ml soluble anti-CD28 antibody (BioLegend ...
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bioRxiv - Immunology 2022Quote: ... Cytokine staining of splenocytes after attLm-OVA infection was carried out after re-stimulation of CD8 T cell enriched splenocytes with 50 nM SIINFEKL peptide for 3 hours with Brefeldin A (Biolegend, cat#:420601).
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bioRxiv - Neuroscience 2022Quote: ... and 0.3% PBST for 3 days at 4°C on rotating incubator (NFH, 1:500, Abcam, ab4680; Tubulin β-III, 1:200, Biolegend, 801201). Whole muscles were washed in 0.3% PBST for 5 hours then incubated in secondary antibodies for 48h at 4°C on rotating incubator (AlexaFluor 568 ...
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bioRxiv - Microbiology 2023Quote: ... Tissue was rinsed 3 times in 1X PBS and then incubated with 1:5000 dilution rabbit polyclonal anti-Keratin-14 antibody (Biolegend, Clone Poly19053) for 1 hour and then detected with 1:10000 dilution goat anti-Rabbit IgG Secondary Antibody conjugated with Alexa Fluor™ 488 (ThermoFisher Scientific ...
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bioRxiv - Immunology 2023Quote: ... Supernatants were collected 3 days after coculture initiation and used to quantify T cell activation levels with ELISA (BioLegend, Cat no. 430801) following the manufacturer’s protocols.
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bioRxiv - Immunology 2023Quote: ... then were resuspended in FACS (PBS + 3% FBS + 1mM EDTA + 10mM HEPES) supplemented with TruStain FcX (1:50; BioLegend; San Diego, CA) and TruStain Monocyte Blocker (1:20 ...