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Citations for BioLegend :
301 - 350 of 613 citations for Dengue Virus Serotype 3 DIII envelope protein since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2024Quote: ... program cell death protein ligand 1 (PD-L1; clone: 10F.9G2; BioLegend). Samples were fixed and permeabilized with Fix/Perm buffer according to the manufacturer’s instruction (eBioscience ...
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bioRxiv - Immunology 2024Quote: ... Protein loading control HRP was detected using anti-GAPDH (clone W17079A; BioLegend). The blots were then washed 3 times with TBST and incubated with secondary antibody ...
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bioRxiv - Immunology 2022Quote: ... α-CCR10-APC (clone 6588-5) and α-TIM-3-PE-Cy7 (clone F38-2E2) from BioLegend. α-ULPB2/5/6 (mouse ...
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bioRxiv - Cancer Biology 2021Quote: ... and supplemented with murine SCF (10 ng/ml) and IL-3 (10 ng/ml) (Peprotech or BioLegend). The cells were serially passaged for ∼1 month ...
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bioRxiv - Biochemistry 2020Quote: ... Cells were blocked with PBS containing 3% BSA and stained with Alexa Fluor 647-anti-DYKDDDDK (BioLegend) at 0.5 μg/mL or with Alexa Fluor 488-streptavidin (Life Technologies ...
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bioRxiv - Molecular Biology 2021Quote: ... incubated with secondary antibody diluted in 3% BSA (Alexa Fluor 594 Goat anti-Rat IgG, BioLegend, #405422) in the dark for 1 hr at room temperature ...
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bioRxiv - Immunology 2023Quote: ... we used Ultra-LEAF™ purified anti-human TIM-3 antibody (10μg/ml, clone F38-2E2, Biolegend) or its respective isotype Ultra-LEAF™ purified mouse IgG1 antibody (10μg/ml ...
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bioRxiv - Cell Biology 2022Quote: ... cells were incubated with Alexa Fluor 488 anti-mouse/human Mac-2 (Galectin-3) antibody (125410, Biolegend) or alternatively with Brilliant Violet 421 anti-mouse/human Galectin-3 antibody (125416 ...
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bioRxiv - Microbiology 2024Quote: ... and neurons were labelled with rabbit anti-tubulin beta 3 (TuJ-1) IgG at 1:500 (Biolegend), in PBS ...
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bioRxiv - Physiology 2022Quote: ... Samples were stained with 3 μM of (4’,6-Diamidino-2-Phenylindole, Dilactate (DAPI, Biolegend Cat# 422801)) ...
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bioRxiv - Cancer Biology 2023Quote: ... one million cells were harvested 3 days after transduction and washed twice with cell staining buffer (Biolegend). Cells were resuspended in 100μL of Cell Staining Buffer (Biolegend ...
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bioRxiv - Cell Biology 2024Quote: ... samples were permeabilized with the Intracellular Staining Perm Wash Buffer for 3 x 5 min (421002, BioLegend), followed by blocking in Superblock solution (Invitrogen ...
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bioRxiv - Immunology 2024Quote: ... Plates were washed with PBS and coated for >3 h with 1 µg/mL αCD3 (HIT3a, Biolegend) at 37°C ...
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bioRxiv - Immunology 2024Quote: ... ELISA plates were coated with purified antibody (3 µg/ml) overnight at 4°C (BioLegend, catalog 519202). The next day ...
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bioRxiv - Cancer Biology 2024Quote: ... Cells were allowed to proliferate for 3 days before collecting and staining with CD3e-PE (BioLegend 100308) and CD11b- PECy7 (BioLegend 101216 ...
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bioRxiv - Immunology 2024Quote: 3−5×107 PBMC were stained using a cocktail of antibodies that included CD3 (clone UCHT1, Biolegend), CD4 (clone OKT4 ...
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bioRxiv - Immunology 2020Quote: ... Recombinant mouse (rm) M-CSF and GM-CSF proteins were obtained from BioLegend. MOG35-55 peptide (MEVGWYRSPFSRVVHLYRNGK ...
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bioRxiv - Systems Biology 2021Quote: ... surface proteins were stained with a cocktail of 53 TotalSeq-C antibodies (Biolegend). Antibody concentrations were either predetermined by titration (Kotliarov et al. ...
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bioRxiv - Immunology 2022Quote: ... Antibodies with specificity for the following cell surface proteins were purchased from Biolegend with different fluorochrome labels ...
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bioRxiv - Microbiology 2020Quote: ... Protein G agorose beads that were pre-adsorbed to either anti-HA (BioLegend) or anti-FLAG (Millipore ...
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bioRxiv - Immunology 2022Quote: ... CD46 APC (targets MCP protein) clone TLA-2-10 (cat# 352405, BioLegend®), CD49d APC (targets α4 integrins ...
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bioRxiv - Immunology 2022Quote: ... Protein concentrations of inflammatory cytokines were determined by LegendPlex™ Multiplex Assay (BioLegend) using the pre-defined mouse inflammation panel according to the manufactureŕs instructions ...
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bioRxiv - Immunology 2021Quote: ... and cells were stained with antibodies against surface proteins CD3 (APC-fire, BioLegend), CD11b (APC Cy7 ...
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bioRxiv - Immunology 2023Quote: ... whereas ADT libraries (cell surface protein libraries) were processed following instructions from Biolegend. 3’ gene expression and ADT libraries were quantified by fragment analyzer and pooled for multiplexed sequencing on a NovaSeq 6000 instrument by the Deep-sequencing Facility at the Max Planck Institute for Immunobiology and Epigenetics ...
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bioRxiv - Immunology 2024Quote: ... biotinylated gE proteins (Acro Biosystems) were conjugated with BV421- or APC-streptavidin (Biolegend) at a molar ratio of 4:1 ...
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bioRxiv - Immunology 2022Quote: ... Antibodies to detect immunoprecipitated proteins were as follows: Eos (1:500, Biolegend, W15169A), STAT5 (1:5000 ...
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bioRxiv - Biochemistry 2024Quote: ... Alexa Fluor 488-conjugated anti- Glial fibrillary acidic protein (GFAP) Antibody (644704, Biolegend), and Alexa Fluor 594- conjugated Anti-Calbindin Antibody (88831 ...
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bioRxiv - Microbiology 2020Quote: ... The blot was then washed 3 times in PBS-T and probed with goat anti-rat HRP (Biolegend) and goat anti-mouse HRP (Biolegend ...
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bioRxiv - Immunology 2022Quote: ... 96-well ELISA plates were coated with 3 μg/ml GPI-RBD-GM-CSF in coating buffer (BioLegend) overnight at 4 °C ...
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bioRxiv - Cell Biology 2021Quote: ... Cells were washed 3 times in FACS buffer and incubated in Cyto-Fast Fix/Perm Buffer (BioLegend, 426803) for 20 minutes at room temperature ...
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bioRxiv - Immunology 2020Quote: ... frozen PBMC were thawed and stained with 3 μl of anti-human CD5-APCeF780 antibody (clone L17F12, Biolegend), 2 μl of anti-human CD19-PECy7 antibody (clone HIB19 ...
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bioRxiv - Immunology 2021Quote: ... The first surface marker panel included: BV421 and PerCP/Cy5.5 anti-TIM-3 (clones B8.2C12 and RMT3-23, BioLegend), BV510 anti-PD-1 (EH12.1) ...
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bioRxiv - Physiology 2022Quote: ... 3 106 cells were then incubated with Fc receptor blocking solution (Human Trustain FcX, Biolegend, San Diego, USA) for 5 minutes at 4°C ...
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bioRxiv - Cancer Biology 2022Quote: ... 3× before being incubated with APC-conjugated rat anti-CD83 antibody (1.25 μL/10×106 cells; Biolegend, 121510) for 30 minutes on ice ...
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bioRxiv - Cell Biology 2022Quote: ... Both cell fractions were stained with cell hashing antibodies (1:1000, #2 for heart, #3 for blood, Biolegend) and Propidium iodide (1:100 ...
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bioRxiv - Cancer Biology 2023Quote: ... Wells were again washed 3 times before adding 50 µL per well of HRP Streptavidin (#405210, BioLegend, USA) diluted 1:2000 in 1% SureBlock solution and incubated 1 h at RT ...
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bioRxiv - Immunology 2023Quote: ... Cells were plated in a 96-well round-bottom plate coated with 3 μg/ml anti-CD3 (Biolegend), uncoated well or with PMA (1 ng/ml ...
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bioRxiv - Immunology 2024Quote: ... and blocked using 3% FCS or mouse serum in Facs buffer and/or Tru block monocyte blocker (Biolegend) as required ...
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bioRxiv - Synthetic Biology 2024Quote: ... and stained for the intracellular accumulation of IFNγ and TNFα (Supp. Table 3) in 1x Permeabilization Buffer (Biolegend). Samples were analyzed using a Fortessa LSR flow cytometer (BD ...
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bioRxiv - Neuroscience 2022Quote: ... and 3 washes were performed prior to secondary antibody staining with anti-rabbit bv421 1:500 (BioLegend 406410) with 3 additional washes after secondary antibody ...
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bioRxiv - Immunology 2022Quote: ... 3-4 × 106 splenocytes after cell surface staining were fixed with Cyto-Fast Fix/Perm buffer (BioLegend, USA) for 20 min at room temperature and washed twice with FACS staining buffer ...
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bioRxiv - Molecular Biology 2024Quote: ... incubated with secondary antibody diluted in 3% BSA PBS (Alexa Fluor 488 Goat anti-mouse IgG, BioLegend, 405319) in the dark for 1 hour at room temperature and washed 3x with PBST ...
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Most L1CAM is not associated with extracellular vesicles in human biofluids and iPSC–derived neuronsbioRxiv - Neuroscience 2024Quote: ... medium was changed on day 7 to fresh APEL medium supplemented with IL-3 (50 ng/ml) (BioLegend) and G-CSF (10 ng/ml ...
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bioRxiv - Immunology 2024Quote: ... Cells were washed 3 times with PBS containing 2% FBS before 7-AAD viability staining solution (BioLegend, #420404) was added and cells were subjected to FACS (BD FACSAria 4-laser digital flow cytometer with FACSDiva v8 software) ...
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bioRxiv - Immunology 2024Quote: ... seeded in a F-bottom 96-well plate precoated with 3 µg/mL anti-CD3 (145-2C11; Biolegend) using 5×105 cells (200 µl ...
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bioRxiv - Genomics 2024Quote: ... We cultured BxPC-3 cells in R10 only or in R10 supplemented with 100 IU/mL IFNγ (BioLegend) for 2 days (n = 6 biological replicates per condition) ...
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bioRxiv - Microbiology 2022Quote: ... Endotoxin-free recombinant mouse IL-1α and IL-1β proteins were purchased from BioLegend.
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bioRxiv - Cell Biology 2022Quote: ... mouse monoclonal anti-NPC proteins (FXFG repeat-containing nucleoporins) (1:1,000; mAb414; 902907, Biolegend), rabbit monoclonal anti-BANF1/BAF (1:200 ...
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bioRxiv - Immunology 2022Quote: ... Briefly PBMC or IDCs were cultured in presence of a protein transport inhibitor (Biolegend) in media alone (control ...
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bioRxiv - Immunology 2022Quote: ... Cells were than stained with Siglec-5 Fc chimeric protein (Biolegend, San Diego, CA) pre-incubated with anti-human IgG Fc fluorescent antibody (Biolegend ...