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Citations for BioLegend :
501 - 550 of 613 citations for Dengue Virus Serotype 3 DIII envelope protein since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2020Quote: ... Protein bands were either visualized by Comassie stain or by standard Western blot procedure (anti-ubiquitin antibody BioLegend, Cat Number ...
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bioRxiv - Biochemistry 2020Quote: ... bound proteins were stained for 30 minutes with anti-human IgG-APC (clone HP6017, 1/250 dilution; BioLegend) and anti-HIS-FITC (chicken polyclonal ...
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bioRxiv - Immunology 2020Quote: Antibodies and clones reactive against the following proteins were used: CD2 (TS1/8, BV421 or Pacific Blue, Biolegend), CD3 (UCHT1 ...
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bioRxiv - Microbiology 2020Quote: ... rpoS protein was probed by anti-mouse IgG anti-E.coli RNA sigmaS clone:1RS1 antibody (Biolegend; batch-B274469). Protein gel was cut at 50KDa ladder mark ...
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bioRxiv - Immunology 2022Quote: Biotinylated RBD protein (Acro Biosystems, cat# SPD-C82E9) was complexed with fluorochrome-conjugated streptavidin APC (BioLegend, cat# 405207). Lymph node cells from FNA were incubated with the RBD-fluorochrome complex and subsequently stained with aqua live/dead dye (Invitrogen ...
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bioRxiv - Developmental Biology 2021Quote: The following antibodies and dilutions were used: rabbit anti-transformation related protein 63 (P63, 1:250, 619002, Biolegend), rabbit anti-red fluorescent protein (tdT ...
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bioRxiv - Genetics 2023Quote: ... proteins were transferred onto nitrocellulose membranes and probed with a 1:2,000 dilution of anti-RpoS antibody (Biolegend). For secondary antibody ...
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bioRxiv - Cancer Biology 2023Quote: ... and supplemented with sCTLA-4 conditioned media or murine CTLA-4-Fc fusion protein (10 µg/mL, BioLegend). After 4 days ...
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bioRxiv - Immunology 2024Quote: Biotinylated RBD protein (Acro Biosystems, cat# SPD-C82E9) was complexed with fluorochrome-conjugated streptavidin APC (BioLegend, cat# 405207). LN cells from FNA were incubated with the RBD-fluorochrome complex and subsequently stained with Live/Dead dye (Aqua ...
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bioRxiv - Biochemistry 2024Quote: ... The following primary antibodies were used: Alexa Fluor 488-conjugated anti- Myelin Basic Protein (MBP) Antibody (850908, Biolegend), Alexa Fluor 568-conjugated Anti- neuronal nuclei (NeuN ...
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bioRxiv - Biochemistry 2024Quote: ... Human recombinant fibroblast activation protein alpha and dipeptidyl peptidase IV (DPP4) were purchased from Biolegend (San Diego, CA). The single domain antibody ...
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bioRxiv - Bioengineering 2021Quote: ... T cells were similarly isolated and stained with Ghost Dye and lineage/differentiation markers (without CD19) and with TIM-3 BV711 (Biolegend clone F38-2E2), LAG-3 BV421 (Biolegend clone 11C3C65) ...
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bioRxiv - Cancer Biology 2024Quote: ... Spleenocytes were resuspended in 3 ml MojoSort™ buffer and enriched for CD8+ T cells using the MojoSort™ Mouse CD8 T Cell Isolation Kit (BioLegend, 480035), following manufactureŕs instructions.
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bioRxiv - Microbiology 2024Quote: ... IL-10, IL-12p70, IFN-α2, IFN-β, IFN-λ1, IFN-λ2/3, IFN-γ, TNF-α, IP-10 and GM-CSF; BioLegend) assay was performed on patient plasma samples as per the manufacturer’s instructions.
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bioRxiv - Immunology 2024Quote: ... The cells were expanded for additional 3 days and Siglec+ population was sorted by staining cells with anti-Siglec-7 APC (BioLegend, clone 6-434) or anti-Siglec-9 APC (BioLegend ...
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bioRxiv - Immunology 2024Quote: ... all peptide-stimulated PBMCs were stained with TotalSeqTM-A 0390 anti-human CD127 - and TotalSeqTM-C 0085 anti-human CD25 oligo-antibodies (BioLegend, Supplementary table 3) to distinguish between conventional T cells (CD127+CD25- ...
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bioRxiv - Biophysics 2021Quote: Flow channels were constructed as previously described but anti-TAMRA antibody was replaced with Anti-ß3 tubulin antibody (BioLegend Poly18020 anti-Tubulin Beta 3). Microtubule nucleation was achieved by introducing 5-50 μM tubulin (1:17 molar ratio of Atto-633 labelled:unlabelled ...
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bioRxiv - Neuroscience 2020Quote: The cerebellar slices were treated either 3 times 24 hours apart with combinations of recombinant TNF (recombinant rat TNF, Biolegend, San Diego, CA, USA), LTα (recombinant human LT-α ...
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bioRxiv - Cell Biology 2022Quote: ... half a million cells per sample were incubated for 10 min in 50 μl of PBS with 3% FSB and 5 μl of blocking buffer (BioLegend, San Diego, CA, USA) and then stained with 50x diluted TotalSeq-B cell feature barcode antibody (BioLegend ...
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bioRxiv - Cell Biology 2024Quote: ... The following primary antibodies were used in this study: anti-tubulin beta-3 conjugated to Alexa Fluor 488 (BioLegend; AB_2562669; mouse IgG2a, 1:200), Alexa Fluor 555 (BD PharMingen ...
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bioRxiv - Cancer Biology 2023Quote: Other conjugated antibodies used in this study include the following: Alexa Fluor® 647 anti-Tubulin Beta 3 (1:1000, Biolegend, #657406, clone AA10), Alexa Fluor® 488 anti-Vimentin (1:500 ...
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bioRxiv - Bioengineering 2020Quote: ... Levels of TNF-α mRNA and protein were detected by qPCR (Table 1) and ELISA (BioLegend, San Diego, CA), respectively.
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bioRxiv - Biochemistry 2020Quote: ... Tetramers were prepared by mixing 1 or 5 pmol of biotinylated protein monomer with 0.25 to 1.25 pmol of streptavidin-PE (Biolegend #405245) respectively and incubating for 2 hours at room temperature ...
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bioRxiv - Cell Biology 2021Quote: ... Cells were stained with an antibody that recognizes the luminal epitope of the human LAMP1 protein (clone H4A3, BioLegend) conjugated with Alexa fluor 488 or 647 for 30 minutes ...
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bioRxiv - Immunology 2022Quote: ... SARS-CoV-2 biotinylated RBD protein (His & AVI Tag) (SinoBiological, 40592-V27H-B) conjugated with APC-streptavidin (BioLegend, 405207), and 7-AAD (Invitrogen ...
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bioRxiv - Immunology 2022Quote: ... SARS-CoV-1 biotinylated RBD protein (His & AVI Tag) (SinoBiological, 40634-V27H-B) conjugated with PE-streptavidin (BioLegend, 405204), SARS-CoV-2 biotinylated RBD protein (His & AVI Tag ...
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bioRxiv - Immunology 2022Quote: ... PVDF membrane transferred proteins were probed with antibodies against anti-Siglec 5/14 (clone 1A5, Biolegend, San Diego, CA).
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bioRxiv - Pathology 2022Quote: ... was coated with 1.2 μg/mL HERV-K113 Capsid protein (sp|Q902F9|90-632, Genescript) diluted in ELISA coating buffer (421701, Biolegend) at 4 ℃ overnight ...
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bioRxiv - Neuroscience 2022Quote: ... and then incubated 1:100 with antibodies directed against cell surface proteins CD11b-BV421 (BioLegend, 101251, clone M1/70), CD45-Percp-Cy5.5 (BioLegend ...
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bioRxiv - Microbiology 2020Quote: ... staining and neutrophil purity was assessed using a Benton Dickson LSR Fortessa X-20 flow cytometer via distinct forward and side scatter profiles or CD66b-peridinin chlorophyll protein (PerCP)/Cy5.5 (clone G10F5, BioLegend) expression ...
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bioRxiv - Biochemistry 2020Quote: ... HA-Flag-tagged and GFP-tagged proteins were detected using HRP-conjugated monoclonal anti-HA (1:5000, BioLegend #901501) or polyclonal anti-GFP antibodies (1:2000 ...
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bioRxiv - Genomics 2021Quote: ... and then incubated 1:100 with antibodies directed against cell surface proteins CD11b-BV421 (BioLegend 101251, clone M1/70), CD45-APC (BioLegend 103112 ...
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bioRxiv - Bioengineering 2020Quote: ... recombinant AAV9 (2×109 vg/well) and SpCas9 protein (0.27 μg/well) were diluted in 1x Coating Buffer A (BioLegend) and used to coat a 96-well Nunc MaxiSorp plate ...
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bioRxiv - Immunology 2021Quote: Human and mouse IL-6 and TNF-α protein were measured by an enzyme-linked immunosorbent assay (ELISA) (BioLegend) according to the manufacturer’s instructions.
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bioRxiv - Immunology 2022Quote: ... SARS-CoV-1 biotinylated RBD protein (His & AVI Tag) (SinoBiological, 40634-V27H-B) conjugated to PE-streptavidin (BioLegend, 405204), SARS-CoV-2 biotinylated RBD protein (His & AVI Tag ...
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bioRxiv - Cancer Biology 2022Quote: ... Either TotalSeq-A Anti-Nuclei Pore Complex Proteins Hashtag 9 or 10 Antibody (1uL at 1:5 dilution; BioLegend) was added to each aliquot of lysis buffer ...
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bioRxiv - Cell Biology 2022Quote: ... SDS-PAGE–separated proteins were transferred to PVDF membranes and immunoblotted with primary antibodies against IL-1α (503207, Biolegend), IL-1β (12426 ...
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bioRxiv - Immunology 2022Quote: ... or 2 μg/mL nucleocapsid protein (NP) peptide pool in the presence of 0.5 μg/mL anti-CD28 and anti-CD49d mAbs (Biolegend). Cells were incubated at 37°C for 9 hours and BFA was added at 3 h post incubation ...
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bioRxiv - Immunology 2022Quote: ... All enriched samples were stained with the following panel of TotalSeq-A-labeled anti-mouse antibodies to detect protein expression in the scRNA-seq dataset: Ly6C (HK1.4, BioLegend), CD44 (IM7 ...
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bioRxiv - Immunology 2022Quote: ... SARS-CoV-2 biotinylated RBD protein (His & AVI Tag) (SinoBiological, 40592-V27H-B) conjugated to APC-streptavidin (BioLegend, 405207). After washed twice ...
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bioRxiv - Cell Biology 2024Quote: ... The expression level of specific proteins was detected by immunoblot analysis with the following antibodies: HA (BioLegend, MMS-101R), V5 (Thermo Fisher Scientific R96025) ...
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bioRxiv - Neuroscience 2023Quote: ... Primary antibodies used for immunofluorescence on these tissues include: mouse anti-myelin basic protein (MBP) (1:1000; #808401 BioLegend), rabbit anti-MBP (1:200 ...
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bioRxiv - Immunology 2023Quote: ... a 10X molar excess of biotinylated RBD-Avi protein was incubated with 0.5 mg/ml of streptavidin-AlexaFluor 488 or streptavidin-AlexaFluor 647 (BioLegend) for 1 hour at room temperature ...
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bioRxiv - Immunology 2024Quote: ... HVEM binding to BTLA was assessed by incubating cells with the HVEM-Fc recombinant protein (20 µg/ml; Biolegend) followed by a staining with an Alexa488 conjugated-anti-IgG1 secondary Ab (clone HP6069 ...
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bioRxiv - Molecular Biology 2024Quote: ... Membranes were blocked in 5% BSA in Tris-buffered saline/0.05% Tween-20 and proteins were detected by specific primary antibodies against MBP (#SMI 99, 1:1000; Biolegend), MOBP (bs-11184R ...
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bioRxiv - Neuroscience 2020Quote: ... the neurons were fixed and stained as previously described38 using primary antibodies for β-3-Tubulin (TUJ1; 1:200; BioLegend, San Diego, CA, USA; 801201) and TH (1:300 ...
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bioRxiv - Immunology 2021Quote: PBMCs were stained with the following antibodies and proteins for flow sorting: anti-CD19-APC (1:10 dilution, clone HIB19, cat. no. 982406, BioLegend), anti-IgD-FITC (1:20 dilution ...
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bioRxiv - Immunology 2021Quote: ... Eluted fractions were pooled and purified proteins were verified for integrity by probing with an anti-HIS tag antibody (Biolegend) as well as with subtype-specific mAbs via SDS-PAGE and Western blot.
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bioRxiv - Immunology 2021Quote: ... Antibodies for flow cytometry and cell isolation against the following proteins (all mouse unless otherwise indicated) were obtained from BioLegend, eBioscience ...
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bioRxiv - Genomics 2022Quote: ... Cells were stained for the respective cell surface protein for 30 min at 4°C and measured by fluorescence-activated cell sorting (Sony SH800) (BioLegend: CD46 clone TRA-2-10 #352405 - 3μl per 1×106 cells ...