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Citations for BioLegend :
351 - 394 of 394 citations for Protease Inhibitor Cocktail EDTA Free 100X in DMSO since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Physiology 2023Quote: ... zoledronic acid (Novartis Pharmaceuticals) and/or 50 μg/kg (i.p.) recombinant human granulocyte-colony stimulating factor (G-CSF; carrier-free, BioLegend #578604) for three days ...
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bioRxiv - Physiology 2023Quote: Kupffer cells cultured in 96-well plates were supplemented with 30 μg EPO per well (recombinant rat EPO carrier-free, Biolegend). After 1,5 hours ...
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bioRxiv - Cancer Biology 2023Quote: ... Jurkat transfectants were activated for 5 minutes at 37°C in serum-free RPMI-1640-sodium bicarbonate by crosslinking the anti-CD3 antibody (1μg/ml, clone UCHT1, BioLegend #300402) with secondary anti-mouse antibodies for immunoblot analysis of GSK-3 α/β and Akt phosphorylation.
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bioRxiv - Immunology 2023Quote: ... After blocking of free capture sites on the biosensors with an isotype control (10 µg/mL, human IgG1κ, clone QA16A12, BioLegend) for 600 s ...
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bioRxiv - Immunology 2023Quote: ... Tgf-β concentration was measured using ELISA (Total Tgf-β1 Legend MAX and Free Active Tgf-β1 Legend MAX, Biolegend). The limit of detection was 8 pg/mL for Tgf-β1 ...
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bioRxiv - Immunology 2024Quote: Secretion of soluble cytokines and chemokines were assayed from cell-free culture supernatants of in vitro stimulated neutrophils and monocytes using bead-based immunoassays (LegendPlex, Biolegend). Two assays were used allowing the quantification of IL-1β ...
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bioRxiv - Cell Biology 2023Quote: ... Between 50,000 to 100,000 cells were resuspended in 10 uL of PBS supplemented with 2% FBS and 2 mM EDTA (hereafter named “FACS buffer”) together with 5 uL of TruStain FcX (BioLegend, San Diego, CA, USA). Cells were incubated with the FcX block for 10 minutes at RT ...
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bioRxiv - Bioengineering 2022Quote: ... cells were washed with DPBS and harvested with trypsin-EDTA and the viability analyzed by flow cytometry using the Apoptosis Detection Kit with propidium iodide (PI, BioLegend, San Diego, CA, USA) in a Cytoflex flow cytometer (Beckman Coulter ...
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bioRxiv - Cancer Biology 2023Quote: Tumor cell lines and murine splenocytes were stained for flow cytometry assays in FACS buffer (1X PBS, 2 mM EDTA and 2c% FBS) with the following antibody panel: CD3-BV510 (BioLegend, clone 17A2, cat. 100233), NK1.1-PE-Cy7 (BioLegend ...
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bioRxiv - Immunology 2020Quote: ... For investigating the formation of iBALT B cell and T cells were stained in lungs using primary antibody cocktail containing rat anti-mouse CD45R/B220 (BioLegend, Cat#103202) and Hamster anti mouse CD3 (BD ...
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bioRxiv - Bioengineering 2020Quote: ... cells were washed 3x with PBS and incubated with a cocktail of primary antibodies to intracellular compartment markers - clathrin (1:500, Biolegend MMS-423P), caveolin (1:200 ...
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bioRxiv - Immunology 2022Quote: ... for 5 min on ice prior to immunostaining with a surface stain cocktail containing the following antibodies: I-A/I-E Alexa Fluor 488(Biolegend, cat.107616), CD8a PerCP/Cyanine5.5 (Biolegend ...
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bioRxiv - Immunology 2022Quote: ... each sample was pelleted at 1,150 x g in a centrifuge for 5 min at room temperature and then stained with 50 µL of an antibody cocktail containing 1:50 diluted anti-human CD45-AF700 (Biolegend, clone HI30), 1:100 diluted anti-human CD3-FITC (BioLegend ...
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bioRxiv - Immunology 2022Quote: ... each sample was pelleted at 1,150 x g in a centrifuge for 5 min at room temperature and then stained with 50 µL of an antibody cocktail containing 1:100 diluted anti-human CD3-FITC (BioLegend, clone UCHT1), 1:100-diluted anti-human CD4-PE (BioLegend ...
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bioRxiv - Cell Biology 2023Quote: ... Slides were incubated with antibody cocktails in blocking buffer overnight at 4°C in a moisture chamber: Amyloid-β (6E10) (1:100; Biolegend, 803001), anti-CHMP2B (1:100 ...
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bioRxiv - Cancer Biology 2023Quote: ... cells were stained with the following antibody cocktail diluted in flow cytometry staining buffer: mouse anti-human CD3 BV750 (BioLegend Cat 344846), mouse anti-human CD4 AF700 (BioLegend Cat 300526) ...
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bioRxiv - Cancer Biology 2021Quote: ... mice were anaesthetised as described above and intranasally inoculated with 100ng of carrier free recombinant protein (rIL-1α – Biolegend #575002, rIL-1β – Biolegend #575102) suspended in sterile phosphate buffered saline (PBS) ...
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bioRxiv - Molecular Biology 2019Quote: ... and coupled with 10 μg carrier-free anti-epitope tag antibody (c-myc: Santa Cruz #sc-40; FLAG: Sigma #F1804; GluGlu: Biolegend #901801 ...
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bioRxiv - Immunology 2020Quote: Active TGF-β1 serum levels in patients and healthy donors were measured using LEGEND MAX™ Free Active TGF-β1 ELISA Kit with Pre-coated Plates (Biolegend). The assay was run according to the manufacturer’s recommendations.
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bioRxiv - Neuroscience 2023Quote: ... in phenol-red free DMEM-F12 in combination with live-dead stain Zombie NIR (1: 10,000 dilution; Biolegend, cat. no 423106) and incubated for 25 minutes at 37 °C and 5% CO2 conditions ...
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bioRxiv - Genetics 2019Quote: ... The cells were stained with the following antibody cocktail: anti-CD4-APC (30 µl/ml final volume, clone OKT4, Cat. no. 317416, BioLegend, San Diego, U.S.), anti-CD127-FITC and (30 µl/ml ...
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bioRxiv - Neuroscience 2022Quote: ... Splenocytes were then filtered through a 40μM filter and stained with an antibody cocktail [Pacific Blue anti-CD45.1 (Biolegend no. 110722; 1:100 dilution), APC anti-CD45.2 (Biolegend no ...
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Cell therapy with IL-10-producing group 2 innate lymphoid cells suppresses Graft-versus-Host diseasebioRxiv - Immunology 2023Quote: ... Cells were incubated with a TotalSeq-C antibody cocktail as well as hashtag antibodies to allow for post sequencing differentiation of distinct expanded ILC subsets(BioLegend, Supplemental Table 1) for 30min ...
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bioRxiv - Immunology 2023Quote: ... prior to the end of the CD45- sort one vial of TotalSeq-C Human Universal Antibody Cocktail v1.0 (Biolegend, San Diego, CA, USA) was equilibrated to room temperature for 5min and then spun at 10,000g for 30 seconds ...
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bioRxiv - Immunology 2023Quote: ... cells were stimulated for the following 5h using a combination of protein transport inhibitor (Monensin) (Biolegend, USA), Ionomycin (1 ug/ml) ...
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bioRxiv - Cell Biology 2024Quote: ... cells were treated with inhibitors for 6 hours and then stained with Zombie Aqua (BioLegend, Cat. # 423101) following the manufacturer’s instructions.
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bioRxiv - Cell Biology 2021Quote: ... Sorted LSK cells were cultured in StemSpan SFEM serum free media (stemcell technologies) contains 40 ng/ml mSCF (Biolegend, Cat. No. 579702), 40 ng/ml mTPO (Biolegend ...
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bioRxiv - Immunology 2021Quote: ... or restimulated on day 11 in 96-well plates with 2.5ug/ml recombinant SARS-CoV-S protein S1 (Biolegend 792906, carrier-free) for 8h in the presence of 1ug/ml Golgiplug and 0.7ug/ml Golgistop ...
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bioRxiv - Cell Biology 2020Quote: ... Triplicate wells were cultured in complete media or switched to serum free DMEM for 24 hours before screening with Annexin V APC/PI using the Apoptosis detection kit (Biolegend; cat 640932) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2023Quote: ... Then cells were fixed with 4 % methanol-free formaldehyde in HBSS for 10 min prior to staining with either 4 μg/ml anti-ACE2 (Biolegend, clone A20069I) or anti-NRP-1 (Biolegend ...
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bioRxiv - Cell Biology 2020Quote: ... for 10 minutes at 4°C followed by staining with antibody cocktail containing Pacific-Blue-conjugated anti-mouse CD45 (clone 30-F11; Biolegend, San Diego, CA, USA), PE-conjugated anti-mouse Ly-6G (Gr-1 ...
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bioRxiv - Immunology 2022Quote: ... Staining was then performed at 4°C for 30 minutes with a surface stain cocktail containing the following antibodies: CD4 APC (BioLegend, cat. 100412, clone GK1.5), CD8 Alexa Fluor 700 (BioLegend ...
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bioRxiv - Immunology 2021Quote: ... Cells were suspended in FACS buffer and stained for 30 min at 4 °C with the desired antibody cocktails (BioLegend, San Diego, CA, US) of APC-Fire 750-anti CD11b (Cat ...
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bioRxiv - Immunology 2022Quote: ... Staining was then performed at 4°C for 30 minutes with CR3022 or 4A8 and a surface stain cocktail containing the following antibodies: CD169 PerCP/Cy5.5 (BioLegend (San Diego, CA], cat. 142410), SIGN-R1 PE (R&D Systems [Minneapolis ...
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bioRxiv - Genetics 2023Quote: ... The barcoded cells were then collected into a single tube and stained with a cocktail of antibodies: CD33 PECy7 (5 µL per 100 µL stain, Biolegend, cat#303434, clone WM53) and pSTAT3 AlexFlour488 (2.5 µL per 100 µL stain ...
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bioRxiv - Immunology 2022Quote: ... for 4 hours, and blocked with secretory inhibitors, brefeldin A (1:1000, eBioscience) and monensin (1:1000, Biolegend) for the last 2 hours ...
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bioRxiv - Immunology 2022Quote: ... extracellular staining was done in ice-cold buffer (phenol red–free DMEM, 2% FBS) for 30 min using antibodies against CD45 (30-F11; BioLegend, dilution 1:200), CD4 (RM4-5 ...
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bioRxiv - Immunology 2021Quote: ... and stained by intracellular staining for 30 min at 4 °C using a cocktail of the following antibodies: IFN-γ PE (Biolegend, Clone W18272D,1:500), TNF Percp-Cy5.5(Biolegend ...
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bioRxiv - Immunology 2024Quote: ... Yeast cells were washed two times with PBSF and subsequently stained for 30 minutes on ice with a cocktail of anti-HA APC antibody (Biolegend, Clone: 16B12, dilution 1:100), goat F(ab′)2 anti-human IgG PE (SouthernBiotech ...
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bioRxiv - Cancer Biology 2021Quote: ... mice were anaesthetised as described above and intranasally inoculated with 100ng of carrier free recombinant protein (rIL-1α – Biolegend #575002, rIL-1β – Biolegend #575102) suspended in sterile phosphate buffered saline (PBS) ...
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bioRxiv - Immunology 2019Quote: Mice were intranasally dosed with 3 µg in 20 µl per mouse recombinant murine TNF-α (r-TNF-α, carrier-free, 575204, BioLegend Ltd, UK) every day for 3 days ...
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bioRxiv - Immunology 2021Quote: ... isotype controls and inhibitor of VLA-4 were used at 1 μg/ml: LEAF™ purified anti-CD29 (clone HMβ1-1, 102209, BioLegend), LEAF™ purified anti-CD31 (clone MEC13.3 ...
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bioRxiv - Cancer Biology 2023Quote: ... the tumor organoids were treated with the immune checkpoint inhibitor Nivolumab (Selleckchem, Cat#A2002) and the Ultra-LEAF Purified Human IgG4 Isotype Control Recombinant Antibody (Biolegend, Cat#403701) at a concentration of 10 μg/mL ...
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bioRxiv - Immunology 2024Quote: ... 10 ug/mL) or LaAg (5 ug/mL) in the presence of a Golgi complex inhibitor Brefeldin A (5 mg/mL, Biolegend, SanDiego, CA, USA). All centrifugation steps were performed at 4 °C ...