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Citations for BioLegend :
251 - 300 of 394 citations for Protease Inhibitor Cocktail EDTA Free 100X in DMSO since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2021Quote: ... The depleted cell fraction was then stained for 20 min at 4 °C with a cocktail of fluorophore-labeled antibodies CD45-PerCPCy5.5 (1:50) (Biolegend), Ly51-PE (1:800 ...
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bioRxiv - Microbiology 2019Quote: ... EV suspensions were stained with a cocktail of fluorescence-conjugated mAbs (PerCP/Cy5.5-anti-human-CD63, clone H5C6 from Biolegend; RPE-CD11b from Dako ...
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bioRxiv - Cancer Biology 2022Quote: ... cells were pre-incubated for 2 h with the Cell Activation Cocktail containing Brefeldin A according to the manufacturer’s instructions (BioLegend). After surface staining ...
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bioRxiv - Immunology 2022Quote: ... blood leukocytes from Ldlr-/- and T-AbcdkoLdlr-/- mice were stained with a cocktail of antibodies against TCRβ-PB (Biolegend), CD8-PE ...
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bioRxiv - Genomics 2021Quote: ... Isolated 2×106 PBMC cells were incubated with PMA/ionomycin (i.e., stimulated) (Cell Activation Cocktail with Brefeldin A, Biolegend), or DMSO as control (i.e. ...
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bioRxiv - Immunology 2022Quote: ... and stained with a cocktail of fluorescently labeled anti-cytokine antibodies: IFN-γ PE/Dazzle 594 (Biolegend, cat.505845), TNF-α PE/Cyanine7 (Biolegend ...
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bioRxiv - Microbiology 2022Quote: ... Cells were washed one time with FACS buffer and then resuspended in 100 μL of an antibody cocktail containing the following: murine anti-CD45i.v.+ (Biolegend, cat#103112 ...
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bioRxiv - Physiology 2022Quote: ... Cells were centrifuged at 350xg for 2.5 minutes and re-suspended in a primary antibody cocktail including CD45-APC (BioLegend), F4/80-AF488 (BioLegend) ...
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bioRxiv - Immunology 2023Quote: ... Isolated lymphocytes were stimulated with PMA/ionomycin with Brefeldin A (1:500 or equivalent to 1.3 μM Ionomycin and 81 nM PMA, Cell Activation Cocktail, Biolegend) for 5 h at 37°C with 5% CO2 ...
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bioRxiv - Immunology 2023Quote: ... cells were stimulated with PMA/ionomycin with Brefeldin A (1:500 or equivalent to 1.3 μM ionomycin and 81 nM PMA, Cell Activation Cocktail, Biolegend) for 4 hours ...
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bioRxiv - Cancer Biology 2023Quote: ... After 72 hours of incubation splenocytes were treated for 5 hours with activation cocktail with brefeldin A1 (Biolegend, 423303) before staining for CD45 (Biolegend ...
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bioRxiv - Cancer Biology 2022Quote: ... Cells were also supplemented with a physiological growth factor cocktail consisting of 0.2 ng/ml stem cell factor (SCF, catalogue no. 573902; BioLegend), granulocyte macrophage colony-stimulating factor (GM-CSF ...
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bioRxiv - Neuroscience 2023Quote: ... cells were subject to surface staining by being incubated with a cocktail of anti-CD11b (Biolegend, USA, Catalog #101225), anti-CD45 (Biolegend ...
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bioRxiv - Neuroscience 2024Quote: ... Cells were then blocked on ice with PBS + 7.5% FCS for 15 minutes before staining with antibody cocktails (see key resource table) containing TruStain Monocyte Blocker (Biolegend) in FACS buffer for 45 minutes at 4°C ...
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bioRxiv - Biochemistry 2024Quote: ... and then in a T cell activation analysis cocktail including: anti-mouse CD3 clone 17A2 APC conjugate (BioLegend, 100235), rat anti-mouse CD4 AlexaFluor700 conjugate (BioLegend ...
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bioRxiv - Genomics 2020Quote: ... Cells were then washed once with full staining buffer (3% FBS in PBS with 2 mM EDTA) and surface antigens were stained for 30 min on ice using the following antibodies: gdTCR-BV421 (B1, Biolegend, 331218), CD45-Pacific Blue (HI30 ...
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bioRxiv - Cancer Biology 2021Quote: Wildtype RMC219 and RMC2C cells were harvested and 1 x 106 cells were resuspended in buffer A (PBS 1X, EDTA 2mM, inactivated FCS 1%) and 5uL of Human TruStain FcX (Biolegend, 422301) was added for 10 min at room temperature ...
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bioRxiv - Microbiology 2019Quote: ... Cells were harvested using Trypsin-EDTA and resuspended in FACS buffer (PBS containing 10% FBS and 2mM EDTA) and stained with anti-CD300lf PE clone Tx70 (1:100; BioLegend, #132704) or anti-CD300ld PE clone Tx69 (1:100 ...
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bioRxiv - Immunology 2022Quote: Cryopreserved PBMCs were thawed and washed twice with 10 ml of FACS buffer (1 x PBS containing 2% FBS and 1 mM EDTA) and resuspended in 100 μl of 1x PBS containing Zombie UV live/dead dye at 1:200 dilution (BioLegend, 423108) and incubate at room temperature for 15 minutes ...
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bioRxiv - Cancer Biology 2022Quote: ... FACS buffer recipe was D-PBS + 5% FBS + 2% BSA + 5 mM EDTA + 50 µg/ml DNase-I with Human Trustain (Biolegend, 422302). All the compensation was done using UltraComp eBeads™ Compensation Beads (Invitrogen ...
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bioRxiv - Immunology 2023Quote: ... Cells were pelleted at 500xg for 5 minutes and then resuspended in 50uL of PBS+ 2% HI-FBS + 2mM EDTA in the presence of Fc receptor blocking antibody (BioLegend, 101302). Cells were labelled with antibodies as described below ...
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bioRxiv - Immunology 2023Quote: ... Cells were stained with Zombie-violet or Zombie-NIR in PBS at 1:2000 dilution for 5 minutes at room temperature prior to resuspending in PBS + 2% HI-FBS + 2mM EDTA in the presence of Fc receptor blocking antibody (BioLegend, 101302) for blocking ...
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bioRxiv - Immunology 2023Quote: ... Cells were rinsed with FACS buffer (PBS containing 0.5% BSA and 2mM EDTA) for 3 min at 300g and incubated with Fc Receptor Blocking solution (Human TruStain FcX™, Biolegend) for 10 minutes prior to cell surface staining with fluorescently labeled antibodies in Brilliant Stain buffer (BD Biosciences ...
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bioRxiv - Immunology 2023Quote: ... buffer (PBS containing 2% FBS and 2 mM EDTA) and then incubated with human Fc receptor block (1:100 dilution, BioLegend, 422302) for 15 minutes on ice ...
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bioRxiv - Immunology 2024Quote: ... Cells were washed once with PBS supplemented with 1mM EDTA and resuspend in FcR blocking buffer using Human TruStain FcX (Biolegend, 422302) and Mouse TruStain FcX PLUS (Biolegend ...
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bioRxiv - Immunology 2024Quote: ... Cells were then washed with FACS buffer (PBS+ 2% (v/v) FBS with 1mM EDTA) and incubated with a 1:500 dilution of TruStain FCX (Biolegend, 101320) for 5 minutes ...
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bioRxiv - Cell Biology 2024Quote: ... A total of 1 x 106 cells were resuspended in 10 uL of PBS supplemented with 2% FBS and 2 mM EDTA (hereafter named ‘FACS buffer’) together with 5 uL of TruStain FcX (BioLegend, 422301). Cells were incubated with the FcX block for 10 minutes at RT ...
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bioRxiv - Physiology 2022Quote: ... Organoids were harvested in cold Serum-Free Defined Medium media and incubated with Zombie (BioLegend 423107, 1:100) for 15 minutes in the dark at room temperature ...
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bioRxiv - Immunology 2023Quote: ... freshly sorted cells were stained ex vivo or after stimulation with carrier-free recombinant mouse IL-25 (Biolegend) at a concentration of 100 ng/ml in complete RPMI medium for 36 h ...
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bioRxiv - Neuroscience 2024Quote: ... Sections were then incubated with rat–anti-Ly6G (1.25 μg/mL on-slide, 0.67 μg/mL free-floating, 127602; Biolegend), anti-Iba1 (2 µg/mL on-slide ...
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bioRxiv - Immunology 2022Quote: ... Briefly PBMC or IDCs were cultured in presence of a protein transport inhibitor (Biolegend) in media alone (control ...
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bioRxiv - Cell Biology 2020Quote: ... Unlabeled cells were then centrifuged at 300 x g and labeled with a cocktail of 1 μg/ml of PerCP-Cy5.5 anti-EPCAM antibody and Zombie Aqua viability stain (Biolegend 423101) diluted 1:400 from stock concentration in FACS buffer.
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bioRxiv - Cell Biology 2020Quote: ... followed by incubation on ice with labeling cocktail consisting of 1 μg/ml of PerCP-Cy5.5 anti-EPCAM antibody and Zombie Aqua viability stain (Biolegend 423101) diluted 1:400 from stock concentration in FACS buffer ...
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bioRxiv - Immunology 2021Quote: 1-2 × 106 fresh decidual leukocytes were washed with PBS and stained using the following cocktail of antibodies: CD45 (HI30, Biolegend), CD66b (G10F5 ...
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bioRxiv - Cell Biology 2022Quote: ... A cocktail of Abs for surface markers at the predetermined concentrations (CD31-APC 1:100, Biolegend; CD45-APC 1:200, Biolegend, CD117-APC 1:100 ...
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bioRxiv - Immunology 2021Quote: ... 2×106 single splenocytes or BAL cells were incubated for 4 hours at 37° C in the presence of PMA/Ionomycin and Brefeldin A (Cell Activation Cocktail; BioLegend) or GolgiPlug control (BD Biosciences) ...
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bioRxiv - Bioengineering 2020Quote: ... cells were first incubated in a cocktail of PE anti-mouse CD169 and PE anti-mouse F4/80 antibodies (BioLegend). After washing ...
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bioRxiv - Cell Biology 2021Quote: ... cells were re-suspended in RPMI1640 growth media supplemented with T cell stimulation cocktail (4975-03, eBioscience Inc., San Diego, CA) and brefeldin A (Biolegend) and incubated at 37°C for 4 hr.
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bioRxiv - Immunology 2021Quote: ... Cells were stimulated for 3 hours at 37 °C with complete IMDM medium with 1x Cell Activation Cocktail (with Brefeldin A, Biolegend). After stimulation ...
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bioRxiv - Immunology 2022Quote: ... at a concentration of 1 × 106 cells per 100 μl in RPMI medium supplemented with 10%FBS and stimulated for overnight with Cell activation cocktail (Cat:423301, Biolegend) at 37°C incubator ...
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bioRxiv - Bioengineering 2022Quote: ... Streptavidin-coated nanovials were reconstituted at a five time dilution in Washing Buffer containing 140 nM (20 μg/mL) of each biotinylated antibody or cocktail of antibodies: anti-CD45 (Biolegend) and anti-IFN-γ (R&D Systems) ...
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bioRxiv - Immunology 2022Quote: ... cells were transferred to a non-CD3 coated plate and incubated for an additional three days before activation with Cell Activation cocktail with Brefeldin A (Biolegend) for 6 h and collection ...
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bioRxiv - Molecular Biology 2020Quote: ... MNCs were harvested by ficoll gradient centrifugation and human hematopoietic cells were identified by flow cytometry using the following antibody cocktail: HLA-A/B/C FITC (clone W6/32, Biolegend), mouse CD45.1 PE-CY7 (clone A20 ...
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bioRxiv - Immunology 2021Quote: ... The panel for the cohort that received the SARS-CoV-2 spike prime followed by the RBD homotrimer cocktail boost also included SARS-CoV RBD-APC (streptavidin-APC from BioLegend) and WIV1-CoV RBD-BV650 (streptavidin-BV650 from BioLegend) ...
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bioRxiv - Cancer Biology 2021Quote: ... cells were sorted by fluorescent assisted cell sorting on the SH800S cell sorter (Sony Biotechnology) using the following antibodies: FITC anti-human lineage cocktail (Cat# 343604, BioLegend) and APC anti-human CD34 (Cat# 343509 ...
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bioRxiv - Neuroscience 2019Quote: ... slides were incubated for 1.5 hrs at RT with a cocktail of secondary fluorescence-conjugated antibody Alexa488 and DAPI (4’,6-diaidino-2-phenylindole, dilactate; BioLegend) to visualize nuclei ...
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bioRxiv - Immunology 2019Quote: ... Naive CD4+ and CD8+ T cells were isolated using a negative selection cocktail containing the following biotinylated mouse antibodies (Biolegend): TCR γ/δ (clone GL3) ...
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bioRxiv - Genomics 2020Quote: ... 1.25 × 10^6 PBMCs were spun down and resuspended in 25 μl CITEseq antibody cocktail (TotalSeq™-A Antibodies, BioLegend) containing among others one hashing antibody per each sample ...
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bioRxiv - Cell Biology 2021Quote: ... clone HIT2), Lineage marker cocktail (BV510, CD3/14/16/19/20//56, clone OKT3/M5E2/3G8/HIB19/2H7/HCD56) (BioLegend) and PLXDC2 (PE/APC ...
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bioRxiv - Immunology 2020Quote: ... Splenocytes at (5 × 104 per well) were incubated with PMA (0.01 μM) /Ionomycin (0.167 μM) cocktail (BioLegend, San Diego, CA) as a positive control ...