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Citations for BioLegend :
201 - 250 of 394 citations for Protease Inhibitor Cocktail EDTA Free 100X in DMSO since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2020Quote: Heparinized whole blood was used for flow cytometry using the following antibody cocktail: CD45-PerCP (Biolegend 103130), CD3-eFLUO450 (eBioscience 48-0032) ...
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bioRxiv - Genomics 2021Quote: ... Cells sorted for CTLA-4 were treated with Cell Activation Cocktail without Brefeldin A (Biolegend, Cat #423302) for 4 hours prior to fixation ...
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bioRxiv - Genomics 2021Quote: ... Cells sorted for IL-2 were treated with Cell Activation Cocktail with Brefeldin A (Biolegend, Cat #423304) for 4 hours prior to fixation ...
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bioRxiv - Genomics 2021Quote: ... Resultant cell pellets were resuspended in 50ul of antibody cocktail (all antibodies from Biolegend, Supplementary File 12) for 30 minutes before further washing and fixation in 2% paraformaldehyde ...
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bioRxiv - Genomics 2020Quote: ... The cocktail was then added to 1×106 PBMCs pre-stained with Trustain FcX (Biolegend cat. 422302) (Supplementary Figure ...
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bioRxiv - Immunology 2020Quote: ... and then re-suspended in an intracellular staining cocktail of: α-CD3 (17A2, 100249, Biolegend, CA, USA), α-Foxp3 (FJK-16s ...
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bioRxiv - Immunology 2022Quote: ... in addition to a lineage cocktail in PeCy7 (B220 clone RA3-6B2 Biolegend, CD3, clone 17A2 Biolegend, CD11c clone N418 ...
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bioRxiv - Cancer Biology 2023Quote: ... Cells were then stained with a cocktail of antibodies against CD45.2 (APC anti-mouse CD45.2 [104], Biolegend); CD45.1 (Brilliant violet 510™ anti-mouse CD45.1[A20] ...
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bioRxiv - Immunology 2024Quote: ... Donor 5 and 6 PBMCs were stained with a cocktail of anti-human CD3 PerCP-Cy5.5 (Biolegend), CD8 PerCP-Cy5.5 (Biolegend) ...
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bioRxiv - Bioengineering 2024Quote: ... and 0.01% NaN3 (Sigma-Aldrich)] and labeled for 1 h on ice with an antibody lineage cocktail containing CD3-BV510 (UCHT1, Biolegend), CD14-BV421 (M5E2 ...
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bioRxiv - Immunology 2021Quote: ... Extracellular staining was performed in FACS buffer (PBS + 2% FBS + 2mM EDTA) with the following antibodies: CD4 (GK1.5, Biolegend), CD8 (53-6.7 ...
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bioRxiv - Immunology 2021Quote: ... The cells were washed with PBS/BSA/EDTA and stained either with anti-IgM PE (dilution 1:100, Biolegend) and anti-IgG Alexa FluorTM 647 (dilution 1:600 ...
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bioRxiv - Cell Biology 2019Quote: ... with 2□mM EDTA for flow analysis or resuspended in PBS (pH 7.2) with 2□mM EDTA and 1□μg/ml DAPI (Biolegend) for sorting ...
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bioRxiv - Immunology 2022Quote: ... before leukocytes were separated using 30 and 70% Percoll gradients and subsequently stained for 30 minutes in FACS buffer (PBS/2.5%FCS/10mM EDTA/0.01% NaN3) with the following primary antibodies: CD45-PacificBlue (Biolegend, 103126), CD19-biotin (Biolegend ...
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bioRxiv - Cell Biology 2022Quote: ... Cells were then washed with suspension buffer (PBS + 0.5% BSA + 2 mM EDTA + 4.5 mg/mL D-glucose) and resuspended in RBC Lysis Buffer (Biolegend 420301) for one minute at room temperature to remove red blood cells ...
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bioRxiv - Immunology 2023Quote: ... and resuspended in FACS buffer (PBS with 0.5% BSA and 2 mM EDTA) with Fc blocked using human TrueStain FcX (Biolegend) and incubated in the dark for 10 min at room temperature ...
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bioRxiv - Immunology 2023Quote: ... Cells were subsequently resuspended in staining buffer (PBS with 0.5% bovine serum albumin and 2 mM EDTA) and Fc-blocked using TruStain FcX reagent (BioLegend). Cells were then stained with the following fluorophore-conjugated anti-mouse antibodies for 30 min at 4°C ...
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bioRxiv - Immunology 2023Quote: ... cells were resuspended in FACS buffer (PBS with 0.5% BSA and 2 mM EDTA) and Fc blocked using human TrueStain FcX (Biolegend). For cell surface staining ...
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bioRxiv - Developmental Biology 2023Quote: ... then resuspended in ice-cold MACS buffer (DPBS + 0.5% BSA + 2mM EDTA) and incubated with Fc Block (Biolegend 156603) for 5 min at 4°C ...
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bioRxiv - Neuroscience 2023Quote: ... Samples were resuspended in 50 μl of ice-cold flow buffer (0.5% BSA, 1 mM EDTA, in 1x PBS, Sterile Filtered) containing antibodies targeting Cd11b (BV510, clone ICRF44, Biolegend), CD45 (APC/Cy7 ...
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bioRxiv - Immunology 2023Quote: ... 1X protein transport inhibitor (Fisher Healthcare) and 1:1000 PMA/Ionomycin (Biolegend) were added ...
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bioRxiv - Immunology 2021Quote: ... or with cell activation cocktail with PMA (20.25 pM) and ionomycin (335 pM) and 0.15 μg/ml of brefeldin A (Biolegend). Cells were stained with viability dye (Invitrogen ...
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bioRxiv - Immunology 2021Quote: ... at room temperature and resuspended in 50 μL ICS antibody cocktail within Permeabilization Wash Buffer (1x) (PWB) (BioLegend) for 30 mins in the dark at room temperature ...
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bioRxiv - Bioengineering 2020Quote: ... The non-macrophages cell fraction was then incubated in mouse CD4+ T cell isolation biotin-antiboy cocktail (BioLegend) and sorted ...
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bioRxiv - Cell Biology 2022Quote: ... and the fluorophore-conjugated antibody cocktail was added in 1:1 ratio (EpCAM, Clone 9C4, PE-Cy7, BioLegend, catalog number 324222 ...
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bioRxiv - Cell Biology 2022Quote: ... followed by staining for 20 min on ice with a fluorescent antibody cocktail containing anti-CD11c-PE (BioLegend), anti-CD11b-APC-eflour780 (eBiosciences) ...
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bioRxiv - Immunology 2022Quote: ... and stained for 30 min on ice with an antibody cocktail consisting of APC/Cy7-CD3 (SK7, Biolegend), PE/Cy7-CD4 (A161A1 ...
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bioRxiv - Cell Biology 2020Quote: ... a cocktail mixture containing the following antibodies was used: (1) APC conjugated anti-mouse CD11b (1:200; BioLegend), CD31 (1:200 ...
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bioRxiv - Immunology 2020Quote: ... Cells were placed in V-bottom plates and stimulated with 500X Cell Activation Cocktail with Brefeldin A (Biolegend) in IMDM medium supplemented with 8% FCS ...
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bioRxiv - Cancer Biology 2023Quote: ... cells were labeled with an antibody cocktail containing Anti-CD14-PE/Cy7 (Clone 63D3, 367111, BioLegend, 1:100), Anti-CD80-APC (Clone 2D10 ...
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bioRxiv - Cancer Biology 2023Quote: ... were stimulated with 500x Cell Activation Cocktail containing 40.5 μM phorbol-12-myristate 13-acetate (PMA) and 669.3 μM ionomycin (Biolegend) in the presence of 5 μg/mL BFA (Biolegend ...
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bioRxiv - Cancer Biology 2023Quote: ... harvested cells were incubated in Roswell Park Memorial Institute medium with cell activation cocktail with brefeldin A (Biolegend) for 4.5 hours at 37°C ...
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bioRxiv - Immunology 2023Quote: ... injections of a TH17 priming cocktail (n=8; 0.5μg rmIL-6 [575704, BioLegend, USA] and 1μg rmTGF-β1 [763104, BioLegend, USA]) or rest (n=8 ...
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bioRxiv - Immunology 2024Quote: ... single-cell suspensions were stimulated with cell activation cocktail (including phorbol 12-myristate 13-acetate and Ionomycin) (BioLegend) in the presence of Golgi Stop (monensin ...
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bioRxiv - Cancer Biology 2019Quote: ... or 1 million cells (analysis)No in FACS buffer (PBS with 2% FBS, 1X pen/strep and 1 mM EDTA) containing human TruStain FcX (Biolegend) and mouse TruStain FcX (anti-mouse CD16/32 ...
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bioRxiv - Immunology 2021Quote: ... cells were washed in 1xPBS supplemented with 0.5% bovine serum albumin (BSA) and 2 mM EDTA (FACS buffer) and stained with Zombie Aqua Fixable Viability kit (Biolegend) and incubated for 20 min at 4°C followed by surface staining for 30 min at 4°C ...
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bioRxiv - Cancer Biology 2022Quote: ... Blood was collected in EDTA-coated tubes (16.444, Microvette®) to assess immune cell depletion and incubated with RBC lysis buffer (42031, BioLegend) for 5 min at room temperature (RT) ...
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bioRxiv - Microbiology 2020Quote: ... After incubation the cells were washed twice in FACS buffer (PBS, 0.5% BSA, 2 mM EDTA) and stained with a CD19-APC antibody (BioLegend # 302212) to analyze pDCs for 20 min at 4°C to analyze B cells or with two antibodies directed against CD303(BDCA2)-APC (Miltenyi Biotec ...
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bioRxiv - Immunology 2020Quote: ... for 30’ at RT in PBS containing 2% FCS and 1 mM EDTA prior to secondary staining with streptavidin-BrilliantViolet 510 (Biolegend) for 15’ on ice ...
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bioRxiv - Immunology 2020Quote: ... single-cell IEL suspension was incubated with staining buffer (PBS containing 2% FBS with 2 mM EDTA) containing a mixture of biotin-conjugated mAbs against CD4 (RM4-5; BioLegend), CD19 (6D5 ...
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bioRxiv - Immunology 2020Quote: ... Cells were resuspended in 2 mM EDTA in PBS and stained with cell viability dye (Zombie Aqua or Zombie UV, BioLegend). Cells were then incubated with 10 μg/mL anti-CD16/CD32 (2.4G2 ...
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bioRxiv - Immunology 2022Quote: ... 100μg/ml streptomycin and 2 mM EDTA) and stained for 20 minutes at 4 degrees with the following fluorescently conjugated antibodies (BioLegend): anti-CD11c (clone N418) ...
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bioRxiv - Pathology 2022Quote: ... The pellet was further washed once with FACS buffer (0.5% BSA/0.5 mM EDTA in PBS) and stained with APC-conjugated anti-mouse CD45 (1:300, BioLegend, CAT#103112), BV510-conjugated anti-mouse CD11b (1:300 ...
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bioRxiv - Immunology 2023Quote: ... the cells were re-suspended in flow cytometry staining buffer (PBS containing 4% FBS and 2 mM EDTA) and blocked with TruStain FcX (mouse or human, Biolegend) for 5 min at 4°C ...
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bioRxiv - Cancer Biology 2023Quote: ... Hepatocytes were resuspended in 2 mL FACS buffer (2 mM EDTA, 0.5% BSA in PBS) and stained with Zombie Violet (1:500) (Biolegend #423113), TruStain FcX™ (anti-mouse CD16/32 ...
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bioRxiv - Immunology 2023Quote: ... single cell suspensions were washed with FACS buffer (PBS containing 2% fetal calf serum and 1 mM EDTA) and 1 µL of FcX TruStain PLUS (Biolegend) was added to each sample ...
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bioRxiv - Immunology 2024Quote: ... cells were washed with FACS staining buffer (PBS containing 2% fetal bovine serum [FBS] and 1 mM EDTA) and incubated with an Fc receptor blocker (TrueStain fcX; BioLegend) for 5 minutes at 4℃ ...
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bioRxiv - Cell Biology 2024Quote: ... 2 mM EDTA in PBS) and incubated with Fc-blocking reagent (1:200, TruStain FcX, anti-mouse CD16/32, BioLegend). After washing ...
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bioRxiv - Bioengineering 2024Quote: ... Cells were then washed twice in FACS buffer (PBS + 2% FBS + 1mM EDTA) before staining with anti-pERK1/2 (Biolegend 369506 100x ...
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bioRxiv - Immunology 2020Quote: ... the same lysates were processed using LEGENDplex™ Mouse/Rat Free Active/Total TGF-β1 Assay (BioLegend) according to manufacturer’s instructions ...