Labshake search
Citations for BioLegend :
351 - 369 of 369 citations for Poloxamer 188 solution since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Plant Biology 2021Quote: ... Biotin labeled DNA was detected with 1:5000 solution of an anti-biotin HPR-conjugated antibody (BioLegend, San Diego, California, United States) in TBST with 5% BSA ...
-
bioRxiv - Immunology 2021Quote: ... Individually titrated working solutions containing one of eight hashtag antibodies were added to each of the eight respective patient samples (Biolegend; Table S13) and incubated on ice for 30 minutes followed by three magnet-based washes in FACS buffer ...
-
bioRxiv - Cell Biology 2024Quote: ... Cells was washed with PBS and preincubated with 5 ml of TruStain FcX™ PLUS (anti-mouse CD16/32) Antibody (Fc Receptor Blocking Solution, BioLegend, 156603) for 10 minutes on ice to reduce nonspecific immunofluorescent staining ...
-
bioRxiv - Immunology 2023Quote: ... PerCP/Cy5.5-conjugated anti-mouse Ly6G mAb (Cat. #.: 127615) and 7-AAD Viability Staining Solution (Cat. #.: 420404) were purchased from BioLegend (CA, USA).
-
bioRxiv - Cancer Biology 2024Quote: ... for 2 minutes at room temperature and then cells were washed in PBS and incubated for 15 minutes at room temperature with a PBS solution containing the fixable viability dye Zombie VioletTM (Biolegend, 1:100) and CD16/CD32 Fc Block (BD Biosciences ...
-
bioRxiv - Developmental Biology 2024Quote: ... The cells are then resuspended in an antibody cocktail prepared in Cell Staining Buffer containing Fc Receptor Blocking Solution (BioLegend, cat #422301) and Brilliant Stain Buffer (Invitrogen ...
-
bioRxiv - Cancer Biology 2024Quote: ... the pellets were incubated for 1 hour in 60 µL of staining solution (1% BSA and 0.5% Tween-20 in PBS) containing 3 µL of anti-BrdU-FITC monoclonal antibody (Biolegend 364104, clone 3D4). The samples were subsequently washed with 1 mL of 1% BSA and 0.5% Tween-20 in PBS ...
-
bioRxiv - Neuroscience 2020Quote: ... in PBS and then incubated with an Fc receptor blocking solution (Human TruStain FcX, 1:20 in staining buffer; Biolegend, San Diego, CA) for 10 min ...
-
bioRxiv - Immunology 2021Quote: ... Bacteria were then resuspended in 0.2µm-filtered solutions of appropriate secondary reagents (Alexa 647-anti-human IgG, Jackson Immunoresearch 1:200, Brilliant Violet 421-anti-Rabbit IgG, Biolegend 1:200). This was incubated for 10-60 min before cells were washed as above and resuspended for acquisition on a BD LSRII or Beckman Coulter Cytoflex S ...
-
bioRxiv - Biochemistry 2022Quote: ... Next steps of ELISA assay were identical to the ones described with sole exception that instead of o-phenylenediamine dihydrochloride as a peroxidase substrate in these experiments was used TMB high sensitivity substrate solution (BioLegend, San Diego, CA) and absorbance was recorded at 450 nm.
-
bioRxiv - Cancer Biology 2023Quote: ... and stained overnight at 4°C in blocking solution with the following primary antibodies and dilutions: anti-Nestin (1:150, Biolegend, #656802, clone 10C2), anti-EGFR (1:300 ...
-
bioRxiv - Immunology 2022Quote: ... table 1) were diluted in blocking solution (F4/80 [Bio-Rad]: 10 µg/ml, Ly6G [abcam]: 1 µg/ml, MHC-II [BioLegend]: 5 µg/ml), CD163 [Invitrogen] ...
-
bioRxiv - Cancer Biology 2023Quote: ... and stained overnight at 4°C in blocking solution with DAPI and the following antibodies and dilutions: Alexa Fluor® 488 anti-Vimentin (1:500, Biolegend, #677809, clone O91D3), anti-Ki67 (1:300 ...
-
bioRxiv - Cancer Biology 2022Quote: ... the antibody or IgG1 excess was rinsed and pellets were resuspended in APC-conjugated Annexin V at 1:20 in Annexin V buffer solution plus Zombie Violet dye at 1:400 (all antibodies and probes are from Biolegend, San Diego, CA, USA). Samples were transferred into FACS tubes and incubated for 20 minutes at room temperature protected from light prior to their analysis by FACS Canto II (BD ...
-
bioRxiv - Bioengineering 2023Quote: ... wherein each well mixed 50 μL of X2 photobleached conjugated magnetic beads with 50 μL solutions at different concentrations of a recombinant human Interleukin-8 (IL-8) protein (#574202, BioLegend, San Diego, CA, USA). The overall well concentration levels were 0.05 ...
-
bioRxiv - Immunology 2024Quote: ... cells were incubated for 15 minutes at 4 °C in the dark with a combination of Human TruStain FcX Fc Receptor Blocking Solution (BioLegend, San Diego, CA, USA) and Live/Dead™ (Invitrogen ...
-
bioRxiv - Neuroscience 2022Quote: ... Cells were then incubated with the following primary antibodies diluted in the incubation solution overnight: chicken anti-neurofilament NF-M (1:1000) (#822701, BioLegend, USA, previously #PCK-593P, Covance), rat anti-MBP (1:300 ...
-
bioRxiv - Immunology 2024Quote: ... and live/dead staining was carried out with the 7-AAD-Viability Staining Solution (5 µg/ml) according to manufacturer instructions (BioLegend, San Diego, California, United States). Cell Sorting was performed using the CytoFlex SRT Benchtop Cell Sorter (Beckmann Coulter Life Sciences ...
-
bioRxiv - Bioengineering 2024Quote: Cells were washed with PBS and incubated in 5 uL of Human TruStain FcX™ (Fc Receptor Blocking Solution) (Cat. No. 422301, Biolegend, San Diego, California, United States), in 100uL FACS buffer (2% FBS in 1x PBS ...