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Citations for BioLegend :
251 - 300 of 369 citations for Poloxamer 188 solution since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2024Quote: ... Erythrocytes (RBC) were lysed for 10 min at room temperature (RT) using 1x RBC Lysis/Fixation solution (Biolegend). Samples were washed with phosphate buffered saline (PBS ...
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bioRxiv - Bioengineering 2022Quote: ... Sections were rinsed three times in IF solution and incubated with 300 nM DAPI (BioLegend, San Diego, CA) in deionized water for 15 minutes at room temperature protected from light ...
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bioRxiv - Bioengineering 2022Quote: ... The hybridoma sample was stained with a 12 μg/mL solution of TotalSeqC conjugated anti IgG1 antibody (Biolegend) and incubated for 45 minutes on Ice ...
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bioRxiv - Immunology 2022Quote: ... Cells were then incubated in 2% FCS/PBS solution containing anti-mouse CD16/32 antibody (BioLegend clone 93) for 15 minutes and subsequently stained with a combination of fluorophore-conjugated primary antibodies against mouse CD45 (BioLegend clone 30-F11) ...
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bioRxiv - Neuroscience 2022Quote: ... and incubated O/N in 50% block solution with anti-Prox1 1:500 (Biolegend, San Diego, CA; Rb), anti-ChAT 1:1K (Chemicon ...
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bioRxiv - Cancer Biology 2023Quote: ... Resuspended cells were then pooled into a single tube and incubated with Tru-Stain Fc blocking solution (Biolegend) for 10 mins at RT ...
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bioRxiv - Immunology 2023Quote: ... Cells were next treated with human 5µl/tube TruStain FcX™ (Fc receptor blocking solution, Cat# 422302, BioLegend) and incubated for 30 min at RT ...
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bioRxiv - Developmental Biology 2024Quote: ... Stained cells were washed with HBH (Hank Balanced Salt Solution (HBSS) with 0.1% bovine serum albumin (BSA) and 10mM HEPES) and stained with streptavidin-PerCP/Cy5.5 (BioLegend). Stained cells were washed ...
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bioRxiv - Cancer Biology 2024Quote: ... Then blocking solution was aspirated and the cells were incubated with phycoerythrin (PE) anti-FOLR1 antibody (908304, Biolegend) at room temperature for 1h ...
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bioRxiv - Immunology 2024Quote: ... Cells were washed 3 times with PBS containing 2% FBS before 7-AAD viability staining solution (BioLegend, #420404) was added and cells were subjected to FACS (BD FACSAria 4-laser digital flow cytometer with FACSDiva v8 software) ...
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bioRxiv - Immunology 2024Quote: ... CellROX solution was washed and single cell suspensions were stained using surface target antibodies against CD4 (OKT4, Biolegend), CD8 (SK1 ...
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bioRxiv - Cancer Biology 2021Quote: ... tissues were incubated overnight at room temperature with 1 μg.ml−1 DAPI or 0.5 μM Sytox™ Blue solution (Biolegend, catalog no ...
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bioRxiv - Cancer Biology 2021Quote: ... Tissue for sequencing was incubated for 1 hour with 0.5 μM Sytox™ Blue solution (Biolegend, catalog no. 425305) to counterstain cell nuclei ...
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bioRxiv - Cancer Biology 2021Quote: ... Following two washes with PBS (centrifuged at 700 x g for 5 minutes), cells were stained in 200 μL PI staining solution (0.1% Triton X-100, 50 μg propidium iodide (BioLegend), 0.2 mg/mL RNase ...
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bioRxiv - Pathology 2021Quote: ... followed by detection of GM-CSF with HRP-conjugated detection antibody and TMB solution according to the manufacturer’s instructions (Biolegend).
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bioRxiv - Neuroscience 2022Quote: ... Muscle sections were incubated overnight at 4 °C in blocking solution (2 % BSA, 10 % Normal Goat Serum, 0.1 % Triton and PBS) with chicken anti-NFM (#822701, BioLegend, USA ...
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bioRxiv - Immunology 2022Quote: ... and resuspended in 1x Intracellular Staining Perm Wash Buffer (10x stock solution, diluted to 1x with ddH2O, 420801, BioLegend) at 4ºC for 30 min ...
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bioRxiv - Cancer Biology 2022Quote: Mice were injected intravenously (i.v.) with 250 μl of a solution containing 25 μg anti-CD107a PE (ID4B, Biolegend) and 10 μg monensin (Biolegend ...
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bioRxiv - Immunology 2022Quote: ... Brefeldin A solution (420601) and CFSE Cell Division Tracker Kit (423801) were purchased from Biolegend (San Diego, CA, USA). PE anti-mouse IL-4 (12-7041-81) ...
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bioRxiv - Immunology 2023Quote: ... BSA/0.01% (v/v) Tween-20 in cold RNase-free PBS + 10 μl Human TruStain FcX blocking solution (#422301, BioLegend)) and incubated for 10 min at 4°C ...
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bioRxiv - Immunology 2023Quote: ... the cells were harvested and washed twice before subjecting to propidium iodide (PI) solution (10 µg/ml) (BioLegend, #421302) for 10 min ...
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bioRxiv - Cancer Biology 2024Quote: ... The solution was filtered through a 40 μm filter and then placed in 5 mL RBC lysis buffer (BioLegend) for 5 minutes ...
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bioRxiv - Cancer Biology 2024Quote: ... Barcoded cells were combined into a composite sample before incubation in Human TruStain FcX Fc Receptor Blocking Solution (BioLegend) for 10 min at room temperature ...
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bioRxiv - Immunology 2024Quote: ... Stained spleen or LN samples were fixed with 1-4% phosphate-buffered paraformaldehyde solution (FluoroFix or Fixation Buffer, Biolegend). To detect transcription factors and certain intracellular proteins ...
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bioRxiv - Neuroscience 2024Quote: ... a subset of mice were intravenously injected with 0.1mL solution containing 0.4mg/kg FITC anti-mouse CD45.2 antibody (Clone 104, Biolegend, Cat #109806) and 1% Texas Red solution.
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bioRxiv - Bioengineering 2024Quote: ... Cells in each well were centrifuged at 350 g for 5 minutes and resuspended in 100 μL of PBS premixed with 0.5 μL of Trustain FcX plus blocking solution (BioLegend) on ice for 10 min ...
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bioRxiv - Cell Biology 2023Quote: ... The cell pellet was resuspended in 50 µl of the Annexin V Apoptosis Detection Kit/7-AAD solution (BioLegend) and incubated for 15 min ...
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bioRxiv - Cancer Biology 2022Quote: ... washed in blocking solution (2% BSA in PBS) and immunolabeled with anti-CD24 PerCP/Cy5.5 and anti-CD44 FITC primary antibodies (BioLegend, 1:50 ...
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bioRxiv - Bioengineering 2023Quote: ... cells were washed with FACS buffer (PBS + 2% FBS + 0.05% NaN3) and incubated with Fc receptor blocking solution (Human TruStain FcX, BioLegend) for 5 minutes ...
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bioRxiv - Cell Biology 2022Quote: ... one million cells were first resuspended in 100 μL buffer containing 5 μL Fc Receptor Blocking Solution (Biolegend, #422302) and incubated for 15 minutes ...
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bioRxiv - Synthetic Biology 2023Quote: ... resuspended in 50 µl LIVE/DEAD staining solution (PBS with 1:500 Zombie Violet Dye, Biolegend cat. no. 423113) and incubated for 20 min at 4 °C in the dark ...
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bioRxiv - Immunology 2023Quote: ... A 96 well plate was pre-incubated with a 5 µg/ml solution of anti-mouse CD3e (100314, Biolegend) in sterile PBS at 37°C for 2 h ...
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bioRxiv - Immunology 2024Quote: ... + 5 ml FBS + 2mM Ethylenediaminetetraacetic acid [EDTA]) with Human TruStain FcX™ Fc Receptor Blocking Solution (1:20; BioLegend) for 15 minutes at room temperature in foil ...
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bioRxiv - Immunology 2024Quote: ... about 3-4 million CD8+ T cells were blocked with Fc receptor blocking solution (Human TruStain FcX, BioLegend, #422302) for 10 minutes on ice and stained with BEAM-T assemblies for 15 minutes on ice ...
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bioRxiv - Cancer Biology 2024Quote: ... centrifuged at 500g for 5 minutes and resuspended in a solution of 1% bovine ser albumin (BSA) (Biolegend, 644710) in phosphate-buffered saline (PBS ...
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bioRxiv - Neuroscience 2024Quote: ... Retinas were then placed in primary antibody solution containing 1:1000 mouse anti-SMI-32 (BioLegend, Catalog #: 801701, RRID:AB_509997) in 3% normal donkey serum in 0.3% Triton PBS for 2-4 days at 4°C ...
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bioRxiv - Immunology 2024Quote: ... Intracellular stains were performed using commercially available Fix and permeabilization solutions coupled to incubation with Ki-67 and 0.5 µg /mL 7-AAD (Biolegend) or Pmp70/Abcd3.
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bioRxiv - Cancer Biology 2021Quote: ... the cells were stained at the same time with the APC conjugated surface marker antibody CD45 after a 5 min blocking step with Human TruStain FcX solution (20x, BioLegend) diluted in 1 % BSA/PBS ...
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bioRxiv - Immunology 2021Quote: ... cells were subsequently blocked in flow buffer supplemented with either anti-mouse CD16/CD32 antibody (eBioscience) or Human TruStain FcX Fc Receptor Blocking Solution (BioLegend) for 30 min on ice ...
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bioRxiv - Immunology 2021Quote: ... flow buffer was supplemented either with anti-mouse CD16/CD32 antibody (eBioscience) or Human TruStain FcX Fc Receptor Blocking Solution (BioLegend). The following antibodies were used for flow analysis ...
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bioRxiv - Immunology 2020Quote: ... The resulting solution was filtered through a 40 µm nylon mesh and treated with red blood cell (RBC) lysis (BioLegend). Blood was collected by cardiac puncture ...
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bioRxiv - Neuroscience 2020Quote: ... was carefully aspirated with a pipette and placed in 10mL of 1X solution of Red Blood Cell Lysis Buffer (BioLegend) for 15 minutes in the dark ...
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bioRxiv - Neuroscience 2020Quote: For analysis of LILRB2 expression on marmoset and human monocyte derived macrophages were first blocked with human TruStain FcX Fc blocking solution (Biolegend), then labelled with and without anti-human LILRB2 (5 µg/mL ...
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bioRxiv - Bioengineering 2020Quote: ... Staining buffer used was a 1:1000 dilution of stock antibody solution (APC anti-mouse CD45; Alexa Fluor 488 anti-mouse Ly6G, Biolegend) into FACS buffer ...
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bioRxiv - Cancer Biology 2021Quote: ... Then the tissues were washed multiple times in PBS at room temperature for one day and put into a staining solution containing Alexa Fluor 488 anti-mouse CD41 antibody (clone MWReg30, BioLegend) at a 1:100 dilution and Alexa Fluor 555 donkey anti-goat antibody (ThermoFisher ...
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bioRxiv - Cancer Biology 2020Quote: ... Cells were blocked for 10 min on ice following addition of 10 μL Fc blocking solution (BioLegend, cat. no. 422301), 4 μL of a 1% dextran sulfate solution (Research Products International ...
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bioRxiv - Neuroscience 2022Quote: ... Slices were then kept in PBS solution containing Triton X-100 and blocking agent (Normal Serum Block, CAT# 927502, Biolegend) for 2 h after which they were incubated in the solution containing ...
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bioRxiv - Immunology 2020Quote: ... The plates were washed four times with PBS-T and developed with 100 µL/well TMB High Sensitivity substrate solution (BioLegend, UK). After 5 to 10 min the reaction was stopped with 100 µL 1M sulfuric acid and the plates were read at 450 and 570 nm with the Cytation3 Imaging Reader (Biotek) ...
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bioRxiv - Immunology 2020Quote: ... Cells were stained at 4°C for 30 min in Hanks Balanced Salt Solution-2% FBS with the following markers: anti-THY1 AF700 1:100 (Biolegend), anti-PDGFRalpha PE 1:100 (Biolegend ...
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bioRxiv - Immunology 2021Quote: ... The resulting solution was filtered through a 40 µm nylon mesh and treated with red blood cell (RBC) lysis (BioLegend). Blood was collected by cardiac puncture ...