Labshake search
Citations for BioLegend :
201 - 250 of 369 citations for Poloxamer 188 solution since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Cell Biology 2022Quote: ... Cells were washed with PBS and then incubated with Human TruStain FcX Blocking Solution (Biolegend) for 10 mins ...
-
bioRxiv - Immunology 2022Quote: ... cells were washed and blocked using Trustain Fc receptor blocking solution (Biolegend cat. no. 422302) for 10min at RT ...
-
bioRxiv - Cell Biology 2024Quote: ... 100µl aliquots were incubated with 5µl FITC Annexin V and 10µl PI solution (Biolegend 640914) for 15min at room temperature (25°C ...
-
bioRxiv - Bioengineering 2024Quote: ... and pre-treated with a mixture of human TruStain FcX (Fc Receptor blocking solution, Biolegend) and CD16/CD32 Rat anti-mouse Fc antibodies (mouse BD Fc blocker ...
-
bioRxiv - Neuroscience 2024Quote: ... Immunodetection was achieved with the primary antibodies (1:1000 in blocking solution): anti-HA (BioLegend), anti-Flag (Sigma-Aldrich) ...
-
bioRxiv - Biochemistry 2024Quote: ... in the presence of Human TruStain FcX Fc Receptor Blocking Solution (1:400, 422302, BioLegend) for 10min at 4°C in the dark ...
-
bioRxiv - Biochemistry 2024Quote: ... followed by staining with 1 μg/ml propidium iodide solution (BioLegend, San Diego, CA, USA) for 10 min at room temperature ...
-
bioRxiv - Immunology 2021Quote: ... the cells were lysed and fixed with 2 ml of 1x RBC Lysis/Fixation Solution (Biolegend) for 15 minutes at RT and washed with cold FACS-buffer (PBS with 1 % FCS) ...
-
bioRxiv - Immunology 2022Quote: ... Whole blood cell staining was performed using an Fc receptor blocking solution (Human TruStain FcX; BioLegend), the viability dye eFluor780 (Thermo Fisher) ...
-
bioRxiv - Immunology 2022Quote: ... cells were stained for surface markers in the presence of Brefeldin A solution (Cat: 420601, Biolegend) and Monensin solution (Cat ...
-
bioRxiv - Pathology 2022Quote: ... was added to the wells and waited 10 minutes stopped by TMB stop solution (423001, Biolegend). Each well was analyzed for the optical density (OD ...
-
bioRxiv - Immunology 2023Quote: ... samples were incubated with Fc blocking solution (1:100 anti-mouse CD16/32, Biolegend Catalogue # 101302) in FACS buffer (1x PBS with 2% fetal bovine serum) ...
-
bioRxiv - Microbiology 2023Quote: ... The resulting adherent cells were detached from the petri dishes using Accutase cell detachment solution (Biolegend) and seeded into 12 or 24 well plates (Corning ...
-
bioRxiv - Microbiology 2024Quote: ... The resulting adherent cells were detached from the petri dishes using Accutase cell detachment solution (Biolegend) and seeded into 12 or 24 well plates (Corning ...
-
bioRxiv - Molecular Biology 2024Quote: ... one million cells were first resuspended in100LμL buffer containing 5LμL Fc Receptor Blocking Solution (Biolegend, #422302) and incubated for 15Lminutes ...
-
bioRxiv - Immunology 2024Quote: ... human cells were blocked with Human TruStain FcX Fc Receptor Blocking Solution (Biolegend, #422302, 1:100) in FACS buffer and stained with anti-hCD19-PE-Cy7 and anti-hCD138-PE-Cy7 (Biolegend ...
-
bioRxiv - Bioengineering 2023Quote: ... The tubes were centrifuged again and resuspended in 100 μL Zombie Green viability stain solution (BioLegend) at room temperature ...
-
bioRxiv - Developmental Biology 2022Quote: ... live GFP+ cells (Extended Data Fig.1; live stain 1:500 propidium iodide solution (BioLegend #421301)) were sorted on an FACSAria III (BD ...
-
bioRxiv - Genomics 2022Quote: ... resuspended in chilled 1% BSA in PBS and incubated with human TruStain FcX blocking solution (BioLegend) for 10 minutes at 4°C ...
-
bioRxiv - Bioengineering 2024Quote: ... Cells were incubated with Human TruStain FcXTM (FC Receptor Blocking Solution, 1:250, BioLegend catalog #422302) for 5 minutes at room temperature at a 1:250 dilution in DPBS with 1% BSA ...
-
bioRxiv - Cell Biology 2024Quote: ... and blocked with Human TruStain FcX™ Fc Receptor Blocking Solution (1:10 in PBS, Biolegend). Then ...
-
bioRxiv - Immunology 2024Quote: ... cells were stained with an anti-human FcR blocking antibody (human TruStain FcX blocking solution, Biolegend) for 10 minutes at 4°C ...
-
bioRxiv - Cancer Biology 2024Quote: ... After that the lymphocytes were and blocked by Mice TruStain FcX (Fc Receptor Blocking Solution, BioLegend) for 10 min ...
-
bioRxiv - Cell Biology 2020Quote: ... The cells were incubated with primary antibodies diluted in blocking solution: 5% Normal Goat Serum (Biolegend, 927502), or 0.2% (w/v ...
-
bioRxiv - Cell Biology 2020Quote: 100µl bone marrow were blocked with 2µl Fc Receptor Blocking Solution (Human TruStain FcX™, (422301, Biolegend) for 10 minutes at room temperature ...
-
bioRxiv - Neuroscience 2020Quote: ... resuspended them in staining solution containing 1:200 PE goat anti-mouse IgG secondary antibody (#405307, BioLegend) and 1:1000 Hoechst 33342 (Thermo Fisher ...
-
bioRxiv - Cancer Biology 2020Quote: ... Cells were stained with 7-Amino Actinomycin D viability staining solution (7-AAD, BioLegend, San Diego, CA). The suspensions were incubated for 45 min on ice in the dark ...
-
bioRxiv - Cancer Biology 2021Quote: ... organoids were harvested in PBS and digested to a single cell solution in 1:1 Accutase (Biolegend) and TrypLE (Gibco ...
-
bioRxiv - Pathology 2022Quote: ... blood was harvested at the indicated time post infection and treated with 1x RBC lysis solution (BioLegend). Murine Fc receptors were blocked using anti-mouse CD16/CD32 (FcBlock clone 2.4G2 ...
-
bioRxiv - Cell Biology 2022Quote: ... The cells were incubated with antibodies diluted in a blocking solution of 5% Normal Goat Serum (BioLegend) in PBS ...
-
bioRxiv - Biochemistry 2021Quote: ... Cells were resuspended in 25 μL block solution (wash plus 1 in 40 Human TruStain FcX – Biolegend) and incubated for 5 min ...
-
bioRxiv - Neuroscience 2022Quote: ... Pre-centrifuged 50 μL of hashtag antibody solution containing 2 uL of TotalSeq-A Hashtag antibody (BioLegend), with a unique barcode sequence for each iPS cell line ...
-
bioRxiv - Immunology 2021Quote: ... Substrate conversion was stopped after 20 minutes with 100 μL stop solution (2N H2SO4) purchased from BioLegend. Plates were washed with PBS plus 0.05% Tween20 in-between incubations ...
-
bioRxiv - Cancer Biology 2022Quote: ... and cells were incubated with a blocking solution containing mouse TruStain FcX (Biolegend, San Diego, CA; #101319) before antibody staining ...
-
bioRxiv - Immunology 2023Quote: ... serum samples were diluted 2-fold with assay buffer and standards were mixed with matrix solution (Biolegend) to account for additional components in the serum samples ...
-
bioRxiv - Cancer Biology 2024Quote: ... Cells were first incubated with Human TruStain FcX™ (Fc Receptor Blocking Solution) (BioLegend, California, CA, USA) and TruStain FcX™ PLUS (anti-mouse CD16/32 ...
-
bioRxiv - Immunology 2024Quote: ... The cells were incubated with TruStain FcX Fc Receptor Blocking Solution (anti-mouse CD16/32 antibody; BioLegend) to block nonspecific binding to Fc receptors for 10 min on ice ...
-
bioRxiv - Cancer Biology 2024Quote: ... a single-cell solution of lung cells was incubated with Human TruStain FcX (Biolegend, #422302, 1:20) for 10 minutes ...
-
bioRxiv - Immunology 2024Quote: ... 24 h after transfection 0.2×106 cells were fixed with Cyto-Fast Fix/Perm Solution (#750000133, BioLegend) in the dark for 15 min at room temperature and labelled with anti-GFP and anti-RFP primary antibodies in Cyto-Fast Perm/Wash solution 1X (#750000135 ...
-
bioRxiv - Developmental Biology 2020Quote: ... centrifuged in a table top centrifuge at 1,500rpm for 5 minutes and resuspended in 200µl staining solution containing conjugated antibodies Ep-CAM-APC (#118213 Biolegend) and Brilliant violet 785-CD45 (#103149 Biolegend ...
-
bioRxiv - Immunology 2021Quote: ... Binding of Abs was detected by developing with 50µl/well 3,3’,5,5’-tetramethylbenzidine (TMB) high sensitivity substrate solution (Biolegend, UK) and stopping with 50µl 1M sulphuric acid ...
-
bioRxiv - Immunology 2022Quote: ... Cells were then incubated in 2% FCS/PBS solution containing anti-mouse CD16/32 antibody (BioLegend clone 93) for 15 minutes and subsequently stained with a combination of fluorophore-conjugated primary antibodies against mouse CD45 (BioLegend clone 30-F11) ...
-
bioRxiv - Physiology 2022Quote: ... 3 106 cells were then incubated with Fc receptor blocking solution (Human Trustain FcX, Biolegend, San Diego, USA) for 5 minutes at 4°C ...
-
bioRxiv - Microbiology 2020Quote: ... adherent monolayers were detached by incubation at 37°C for 5 mins with Accutase Cell Detachment Solution (BioLegend). After washing ...
-
bioRxiv - Systems Biology 2023Quote: ... before permeabilisation and nuclear staining using PBS supplemented with 2ug/mL DAPI (Biolegend, #422801, stock solution 10mg/ml), 5%FBS (Gibco ...
-
bioRxiv - Immunology 2023Quote: ... was added to the wells for 1 hour at RT prior to developing with 3,3’,5,5’-tetramethylbenzidine (TMB) substrate solution (BioLegend). The reaction was stopped by adding 0.1M sulfuric acid and the optical density was determined at 450 nm using a SpectraMax iD5 spectrophotometer.
-
bioRxiv - Cancer Biology 2024Quote: ... The pooled barcoded sample was stained for 10 minutes at room temperature with Fc receptor blocking solution (BioLegend) followed by 1h incubation with the antibody staining cocktail at room temprature ...
-
bioRxiv - Neuroscience 2024Quote: ... Sections were stained with goat anti parvalbumin antibodies and mounted with Ce3D™ Tissue Clearing Solution (Biolegend, 427704).
-
bioRxiv - Immunology 2024Quote: Splenocytes and peripheral lymph node cells were cultured for 5 h at 37°C in complete medium (1640 RPMI + 10% FBS) plus 0.5 μM OVA257-264 and Monensin solution (1,000×; Biolegend) for cytokines detection ...
-
bioRxiv - Immunology 2024Quote: ... Cells were blocked for 15 min with 10 µL of human Fc receptor blocking solution (TruStain FcXTM; BioLegend). To minimize the interaction of polymeric dyes and improve discrete fluorochrome readout ...