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Citations for BioLegend :
3851 - 3900 of 3905 citations for Human Bcl2 Associated Death Promoter BAD ELISA Kit since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2024Quote: ... the following fluorophore-conjugated antibodies were used at the indicated dilutions: CD117 (c-Kit) BV785 (clone 2B8, BioLegend; 1:200 dilution), Ly-6A/E (Sca-1 ...
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bioRxiv - Cancer Biology 2024Quote: ... KRASG12D and KRASG12D/C185D were stained with FITC-Annexin V and 7-AAD following the instruction of the FITC-Annexin V Apoptosis Detection Kit with 7-AAD (BioLegend; # 640922) and the samples were run on a BD LSRFortessa cell analyzer.
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bioRxiv - Cancer Biology 2024Quote: Spleen was isolated from C57BL/6 mice and CD8 T cells were isolated using MojoSort™ Mouse CD8 T Cell Isolation Kit (Biolegend). The cells were stimulated with Dynabeads™ Mouse T-Activator CD3/CD8 kit (Gibco) ...
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bioRxiv - Cancer Biology 2024Quote: ... on 96-well conical bottom plate and incubated for 15 min in dark at RT in 50 µL of Zombie NIR™ Fixable Viability Kit (Biolegend). After that ...
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bioRxiv - Neuroscience 2021Quote: ... assays were performed using the in situ red starter kit according to the manufacture’s protocol with JADE1 antisera (1:20) and 0N tau antisera (1:500; BioLegend, San Diego, CA). Two control assays were also performed ...
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bioRxiv - Cell Biology 2021Quote: ... Dead cell compensation and gating was completed using a 50 % mixture of live and dead mESCs stained with Zombie Aqua™ Fixable Viability Kit (BioLegend 423101) and incubated in the light-tight container at room temperature for 30 minutes ...
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bioRxiv - Immunology 2020Quote: ... naïve CD4+ T cells were isolated from the spleens of C57BL/6 mice or IL17-GFP mice using a MojoSort™ Mouse CD4 Naïve T Cell Isolation Kit (BioLegend cat. 480040). T cells from C57BL/6 mice were further labeled with CellTrace™ Violet (Invitrogen cat ...
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bioRxiv - Cancer Biology 2021Quote: ... and early/late apoptosis was measured by using the PE Annexin V apoptosis detection kit with 7-AAD (Biolegend, San Diego, USA) according to the manufacturer ...
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bioRxiv - Cell Biology 2020Quote: ... Triplicate wells were cultured in complete media or switched to serum free DMEM for 24 hours before screening with Annexin V APC/PI using the Apoptosis detection kit (Biolegend; cat 640932) according to the manufacturer’s instructions ...
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bioRxiv - Animal Behavior and Cognition 2021Quote: ... A custom rat multi-analyte LEGENDplex bead-based immunoassay kit was used to examine cytokine concentrations in plasma samples (LEGENDplex; BioLegend, CA, USA) that targeted six cytokines concurrently (IL-6 ...
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bioRxiv - Immunology 2020Quote: ... CD4T cells were negatively isolated from the digested spleen and LNs of indicated donor mice with Mouse CD4T Cell Isolation Kit (STEMCELL Technologies 19852 or BioLegend 480033) according to the manufacturer’s protocol and labeled with 1.0 μM CFSE (Thermo Fisher ...
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bioRxiv - Cell Biology 2021Quote: Cytotoxicity of PE-coated and uncoated MNPs on various DC subsets was evaluated using the Zombie Aqua Viability Kit (BioLegend, Fell, Germany) according to manufacturer’ s protocol ...
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bioRxiv - Immunology 2022Quote: ... The following reagents and anti-mouse antibodies were used for flow cytometry: Zombie Violet™ fixable viability kit (BioLegend, San Diego, CA), CD45 (clone 30-F11 ...
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bioRxiv - Neuroscience 2023Quote: ... conjugated using Thermo Fisher Alexa Fluor Kits (AF-488 catalog #A20181, AF-555 catalog #A20187, AF-647 catalog #A20186) to PrP (3F4) (BioLegend catalog #800301) or CD47 (MIAP410 ...
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bioRxiv - Bioengineering 2024Quote: ... were first stained with Zombie Aqua™ fixable viability kit (1:500 dilution in PBS) and Fc-blocked with TruStain FcXTM (anti-mouse CD16/32) antibody (BioLegend, CA, U.S.A.) (1:20 dilution in PBS (1% BSA) ...
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bioRxiv - Cancer Biology 2023Quote: ... CD8+ T cells were isolated from the spleens of C57BL/6 mice using the MojoSort™ Mouse CD8+ T Cell Isolation Kit (Biolegend, #480035), followed by 1X Red Blood Cell lysis buffer (eBioscience ...
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bioRxiv - Neuroscience 2023Quote: CD4+ cells were isolated from pooled splenocytes and lymph node cells with the aid of magnetic beads (MojoSort™ Mouse CD4+ T Cell Isolation Kit, BioLegend #480033) as per the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2023Quote: ... density centrifugation or 1X RBC Lysis Buffer (eBioscience) and stained with a combination of fluorochrome-conjugated antibodies: c-Kit (BD Biosciences, BioLegend clone 2B8), Sca-1 (BioLegend clone D7) ...
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bioRxiv - Immunology 2023Quote: Serum BAFF quantitation was performed on plasma samples from 12-14 week-old Sh2b3E372K mice using the LEGENDplex Mouse B cell Panel – S/P (1-plex) w/VbP kit (BioLegend; cat # 740983) following the manufacturer’s instructions ...
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bioRxiv - Immunology 2023Quote: ... cells were washed twice with PBS and stained using the Zombie NIR Fixable Viability Kit (Biolegend, 423106, diluted 1:500 in PBS) for 30 min at 4°C prior to Fc blocking and staining of surface antigens ...
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bioRxiv - Immunology 2023Quote: CD8+ T cells were isolated from in vitro treated CAR-T cells using Mojosort™ Mouse CD8 T Cell Isolation Kit (Biolegend, 480035). After washing cells with PBS twice ...
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bioRxiv - Genetics 2023Quote: ... were isolated by 1X Red Blood Cell lysis Buffer (eBioscience) and stained with a combination of fluorochrome-conjugated antibodies: c-Kit (BD Biosciences, BioLegend clone 2B8), Sca-1 (BioLegend clone D7) ...
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bioRxiv - Evolutionary Biology 2024Quote: ... or TRIM5α-HA expression (using the CytoFix/Cytoperm kit [BD] according to manufacturer’s instructions and staining with anti-HA-PE [Biolegend] diluted 1:200). Cells were gated on size (FSC vs ...
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bioRxiv - Immunology 2024Quote: ... FSC-A/H and dead cell exclusion by using a Fixable Viability Kit (Zombie Nir™, Biolegend, Cat# 423105, dilution 1:400).
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A resource for extracellular vesicles from activated CD4 T cells that relay pro-inflammatory signalsbioRxiv - Immunology 2024Quote: ... Animals were euthanised using CO2 inhalation and their spleens were aseptically extracted to isolate TH cells employing MojoSort™ Mouse CD4+ T Cell Isolation Kit (Biolegend, #480033). Isolated T cells were cultured in complete RPMI-1640 medium and activated using CD3/CD28 Dynabeads to induce activation ...
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bioRxiv - Bioengineering 2024Quote: Suspended cells prepared as above were stained for live/dead cell quantification with the Zombie NIR Fixable Viability Kit following with the manufacture’s protocol (1:100 dilution, 423111; BioLegend, San Diego, CA). Subsequently ...
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bioRxiv - Cancer Biology 2024Quote: ... Co-culture assays were conducted by incubating SB28 tumor cells with freshly isolated NK cells (BioLegend MojoSort™ NK Cell Isolation Kit) or CD8+ T cells (BioLegend MojoSort™ CD8 T Cell Isolation Kit ...
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bioRxiv - Immunology 2022Quote: ... TF and ICS was performed following permeabilization using True-Nuclear buffer kit according to the recommended protocol (True-Nuclear Transcription Factor Buffer Set; BioLegend, San Diego, CA). Samples were acquired on a Cytek Aurora (Cytek ...
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bioRxiv - Physiology 2022Quote: Apoptotic cells were analyzed by flow cytometry using the FITC Annexin V Apoptosis Detection Kit with propidium iodide (BioLegend, San Diego, CA, USA) following the manufacturer’s instructions ...
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bioRxiv - Physiology 2022Quote: ... and proceeded to Annexin V-fluorescein isothiocyanate (FITC)/PI staining according to the manufacturer’s instructions (FITC Annexin V Apoptosis Detection Kit with PI; BioLegend, San Diego, CA, USA). Cells stained with Annexin V (both Annexin V+- and Annexin V+/PI+-cells ...
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bioRxiv - Immunology 2020Quote: ... DCs were isolated from spleens and lymph nodes of C57BL/6 mice using a MojoSort™ Mouse Pan Dendritic Cell Isolation Kit (BioLegend cat. 480097). DCs were cultured with supernatants of necrotic keratinocytes generated by an overnight culture of UV-irradiated (2 J/m2/s for 40 min ...
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bioRxiv - Microbiology 2022Quote: ... The levels of serum cytokines and chemokines were determined by a bead-based multiplex assay using the LEGENDplex mouse anti-virus response panel (13-plex) kit (#740622, Biolegend, San Diego, USA), according to the manufacturer’s instructions ...
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bioRxiv - Bioengineering 2022Quote: The metabolic activity of HDF cells cultured exposed to the films was analysed using a resazurin assay (Deep Blue Cell Viability Kit (BioLegend, San Diego, USA)) ...
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bioRxiv - Cancer Biology 2022Quote: ... Cells were then stained for viability using Zombie NIR™ fixable viability kit for 15 min at room temperature (Cat # BioLegend, CA, USA), followed by fluorescently labeled high- or low-molecular weight HA (2.5mg/ml in PBS ...
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bioRxiv - Cell Biology 2024Quote: ... naïve CD4 and CD8 T-cells were isolated from syngeneic cryopreserved PBMC aliquots using the MojoSort magnetic negative selection kits (BioLegend, 480041 and 480045). Naïve T-cells were incubated in complete medium supplemented with 10 IU/mL recombinant human IL-2 (R&D Systems ...
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bioRxiv - Immunology 2024Quote: NK cells were enriched from splenocytes from WT or Pvrig KO C57BL/6 mice by using the MojoSort™ Mouse NK Cell Isolation Kit (Biolegend, cat. 480049). Enriched NK cells were cultured and stimulated in vitro in RPMI-1640 Medium (ATCC ...
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bioRxiv - Cancer Biology 2024Quote: ... Spleenocytes were resuspended in 3 ml MojoSort™ buffer and enriched for CD8+ T cells using the MojoSort™ Mouse CD8 T Cell Isolation Kit (BioLegend, 480035), following manufactureŕs instructions.
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bioRxiv - Physiology 2023Quote: CD8+ cells were isolated from the spleens of four 8-week-old diabetic female NY8.3 NOD mice using a MojoSort Mouse CD8 T-cell isolation kit (Cat# 480043, BioLegend, San Diego, CA, USA) and MojoSort buffer (Cat# 480017 ...
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bioRxiv - Immunology 2023Quote: CD3+ T cells were purified from BALB/c mouse spleen cells with the MojoSort mouse CD3 T cell isolation kit (Biolegend, San Diego, CA) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2024Quote: ... CD8 T cells from the CNS were enriched from brain cell pellets post Percoll gradient centrifugation using MojoSort Mouse CD8 T Cell Isolation Kit (BioLegend, cat. no. 480035). For Cre- and Cre+ mice ...
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bioRxiv - Immunology 2024Quote: ... and Propidium Iodide (PI) (1 μg/ml) according to the manufacturer’s instructions (FITC Annexin V Apoptosis Detection Kit with PI, BioLegend, San Diego, CA, USA). The staining of Annexin V requires the usage of a binding buffer for Annexin V as a staining buffer ...
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bioRxiv - Immunology 2021Quote: ... were determined using bead based immunoassays (CBA kit, Cat. 560484, BD PharMingen, San Diego, CA, USA; and LEGENDplex, Cat. 740003, BioLegend, San Diego, CA, USA). Log-transformed data were used to construct standard curves fitted to 10 discrete points using a 4-parameter logistic model ...
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bioRxiv - Cancer Biology 2021Quote: ... Further preparation for FACS measurement was following the protocol of the BioLegend APC Annexin V Apoptosis Detection Kit and DNA-staining with DAPI Reagent (25 μg/mL) (Biolegend, San Diego, CA, USA). 20 000 cells were assayed using a flow cytometer FACSCantoII (Becton Dickinson ...
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bioRxiv - Cancer Biology 2020Quote: ... Cells were resuspended in PBS with 2% FBS containing anti-mouse CD16/CD32 antibody (table 1) and 1:1000 Fixable Viability Dye eFluor™ 506 (eBioscience, Cat#65-0866) or Zombie UV™ Fixable Viability Kit (BioLegend, Cat#423108). Staining with primary fluorophore-conjugated antibodies directed against cell surface markers (Additional file 4 ...
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bioRxiv - Immunology 2021Quote: ... Naïve CD8+ T cells were then negatively-selected (CD8+CD44- purity > 90%) from the resulting single-cell splenocyte suspension using the MojoSort™ Mouse CD8 Naïve T Cell Isolation Kit (BioLegend, San Diego, CA, USA). Two modifications were introduced relative to the manufacturer’s guidelines to increase purity ...
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The loss-of-function of SOCS2 increases the inflammatory response to Staphylococcus aureus infectionbioRxiv - Immunology 2023Quote: ... Individual cytokine detection kits were also used to quantify mouse IL-6 and TNF-α (Bio-techne, France) and GM-CSF (Biolegend, San Diego, USA).
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bioRxiv - Bioengineering 2022Quote: ... cells were washed with DPBS and harvested with trypsin-EDTA and the viability analyzed by flow cytometry using the Apoptosis Detection Kit with propidium iodide (PI, BioLegend, San Diego, CA, USA) in a Cytoflex flow cytometer (Beckman Coulter ...
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bioRxiv - Cancer Biology 2023Quote: Flow cytometry viability staining was conducted by suspending murine spleen and tumor single cell suspensions in Zombie Violet fixable viability kit (Cat # 423114, BioLegend, San Diego, CA, USA) according to manufacturer instructions for 30 minutes at room temperature ...
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bioRxiv - Biophysics 2023Quote: ... Transduced T cells were positively selected with a PE selection kit (EasySep, ref: 17684) and an anti-HA PE-conjugated antibody (Biolegend, ref: 901518, clone: 16B12) at a 1:50 dilution to enrich transduced T cells ...
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bioRxiv - Microbiology 2023Quote: ... the FOXP3/Transcription Factor Staining Buffer kit (eBioscences – 00-5523-00) was used along with the following anti-mouse antibodies: BV605-conjugated anti-CD4 (Biolegend, RM4-5; 1/700), BV650-conjugated anti-B220 (BD Biosciences ...