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Citations for BioLegend :
3651 - 3700 of 3905 citations for Human Bcl2 Associated Death Promoter BAD ELISA Kit since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2021Quote: ... Lung cytokine levels were assessed from the supernatant samples via LEGENDplex kit (mouse inflammation panel 13-plex; BioLegend) per manufacturer’s instructions ...
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bioRxiv - Immunology 2021Quote: Naïve CD4+ T cells were isolated from spleens and lymph nodes of BDC-2.5 transgenic NOD mice using the Mouse CD4 Naïve T Cell Isolation Kit (BioLegend). Naïve T cells were further selected positively by treatment with anti-CD62L biotinylated antibodies (BD Pharmingen ...
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bioRxiv - Immunology 2020Quote: ... Sera were assayed for cytokines using a 13-plex LEGENDplex Mouse Inflammation Panel kit (Biolegend, San Diego, CA) following the manufacturer’s instructions using an Accuri C6 flow cytometer (BD Bioscience ...
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bioRxiv - Immunology 2021Quote: ... CD3+ cell enrichment was achieved by MojoSort negative selection using MojoSort mouse CD3+ T-cell Isolation Kit (BioLegend) according to manufacturer’s instructions ...
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bioRxiv - Immunology 2021Quote: ... the isolated B cells were incubated with 1 μl Zombie UV Fixable Viability kit (Biolegend, cat. no. 423107) per 1 ml cell suspension for 30 min on ice ...
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bioRxiv - Immunology 2022Quote: CD4+ T cells (naïve or total) were enriched with naïve or total CD4+ T cell negative enrichment kits (Stemcell, Biolegend) from the spleens of female ...
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bioRxiv - Systems Biology 2023Quote: ... Dead cells were stained with Fixable Viability Dye eFluor 780 (eBioscience) or Zombie Violet Fixable Viability Kit (BioLegend). Cells were stained with the following fluorochrome-conjugated anti-human Abs ...
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bioRxiv - Immunology 2022Quote: ... Naïve CD4 T cells were presorted with the MojoSort™ Mouse CD4 Naïve T cell Isolation kit (BioLegend) based on provided directions ...
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bioRxiv - Immunology 2022Quote: ... Twelve days post-treatment tumors were dissociated and subjected to CD45.1 positive selection (MojoSort mouse CD45.1 selection kit, Biolegend) per the manufacterer’s instructions ...
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bioRxiv - Neuroscience 2024Quote: Cell suspensions were first stained for viability using Zombie NIR™ Fixable Viability Kit (BioLegend; San Diego, CA) for 20 min at room temperature in phosphate buffer solution (pH 7.2S ...
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bioRxiv - Immunology 2024Quote: ... and IL-6 were measured by LEGENDplex flow-based 13-plex mouse inflammation panel kit from Biolegend (740446) according to manufacturer’s instructions ...
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bioRxiv - Immunology 2024Quote: ... mast cells were stained using CD117 (c-Kit)-APC or APC/Cy7 and FcεRIα-PE (all from Biolegend) at 1:200 dilution for 20 min on ice ...
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bioRxiv - Cell Biology 2023Quote: ... and T cells were isolated from the spleen using the MojoSortTM Mouse CD3 T cell Isolation Kit (Biolegend). Experiments where purified T cells were cultured for longer than 24 hours ...
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bioRxiv - Immunology 2022Quote: ... naïve CD4 T cells were magnetically isolated from the spleen and LNs of Rag2-/- TCR7B8 Tg or TCR1A2 Tg mice on CD45.2/CD45.2 background using the MojoSort™ Mouse CD4 Naïve T cell Isolation kit (BioLegend). Then ...
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bioRxiv - Molecular Biology 2023Quote: ... cells were subjected to a live/dead staining with the Zombie NIR™ Fixable Viability Kit (423105; BioLegend) and incubated in the dark for 10 min ...
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bioRxiv - Immunology 2023Quote: ... CD8+ T cells were isolated by negative selection using the MojoSort Mouse CD8+ T cell isolation kit (BioLegend) and cultivated as described above ...
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bioRxiv - Cell Biology 2023Quote: ... 0.5% BSA and 0.07% NaN3 in PBS) with additional Zombie Aqua™ (1:1,000 Fixable Viability Kit, BioLegend) to stain for vital cells ...
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bioRxiv - Immunology 2023Quote: ... Supernatants were collected after 24h and extracellular IL-2 was measured using the Biolegend ELISAMax Deluxe Kit (Biolegend), according to manufacturer instructions.
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bioRxiv - Neuroscience 2023Quote: ... Cell suspensions were first stained for viability using Zombie NIR™ Fixable Viability Kit (BioLegend, San Diego, CA) for 20 min at room temperature in PBS combined with FcR blocking antibody (1 μg ...
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bioRxiv - Molecular Biology 2023Quote: Cells were trypsinized and stained with Annexin V and 7-AAD using APC Annexin V staining kit (BioLegend) according to the manufacturer’s instructions ...
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Fine tuning of CpG spatial distribution with DNA origami for improved therapeutic cancer vaccinationbioRxiv - Synthetic Biology 2023Quote: ... CD8 OT-I cells and CD4 OT-II were isolated by MojoSort kit purchased from BioLegend (#480035, #480033) according to the manufacturer protocol ...
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bioRxiv - Microbiology 2023Quote: Naïve B cells were isolated from spleens by immunomagnetic depletion using MojoSort Pan B cell Isolation Kit (Biolegend) and cultured in RPMI supplemented with L-glutamine ...
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bioRxiv - Microbiology 2023Quote: ... resuspended in 100 μL Zombie NIR (1:1000 in PBS, Zombie NIR™ Fixable Viability Kit, Biolegend, #423106), transferred into a 96-well plate and incubated for 20 min at 4 °C in the dark ...
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bioRxiv - Bioengineering 2024Quote: ... cells were washed once more with PBS and stained with the Zombie VioletTM Fixable Viability Kit (Biolegend, USA) for 15 min following manufacturer’s instructions ...
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bioRxiv - Bioengineering 2024Quote: ... and cell pellets were washed with PBS before being treated with the Zombie NIRTM Fixable Viability Kit (Biolegend) following the manufacturer’s instructions ...
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bioRxiv - Immunology 2024Quote: ... cells were washed with PBS and stained for dead cells using Zombie UV Fixable Viability Kit (Biolegend, 423108) or Zombie NIR Fixable Viability Kit (Biolegend ...
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Downregulation of Let-7 miRNA promotes Tc17 differentiation and emphysema via de-repression of RORγtbioRxiv - Immunology 2024Quote: CD8+ naïve T cells were isolated from spleen using Mojosort Mouse CD8 Naïve T cell isolation Kit (Biolegend) and adjusted to a concentration of 1.0×106 cells/mL ...
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bioRxiv - Immunology 2024Quote: ... Single cell suspensions were stained with the Zombie NIR™ Fixable Viability KIT (780nm of excitation; Biolegend, #423106) at a 1/250 dilution in PBS (Biolegend ...
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bioRxiv - Cell Biology 2024Quote: ... The samples were subjected to staining using the Annexin V/Propidium Iodide (PI) apoptosis assay kit (BioLegend, USA) and subsequently analyzed using a BD FACSCalibur flow cytometer (USA) ...
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bioRxiv - Physiology 2024Quote: ... using the Pacific Blue Annexin V Apoptosis Detection Kit with 7-amino-actinomycin D (7-AAD) (BioLegend, #640926), according to the manufacturer’s instructions.
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bioRxiv - Microbiology 2024Quote: Single-cell lung suspensions were stained with the Zombie Aqua Fixable Viability kit (1:200 dilution, BioLegend, #423101) and treated with CD16/CD32 Fc block (1:100 dilution ...
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bioRxiv - Immunology 2024Quote: ... CD8 T cells were isolated using the MojoSort Mouse CD8 T Cell Isolation Kit (BioLegend, cat. no. 480035). T cells were washed with fresh PBS 3 times through centrifugation ...
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bioRxiv - Immunology 2024Quote: ... Exclusion of dead cells and determination of cell viability was performed using Zombie UV Fixable Viability Kit (BioLegend). Surface antigen staining was performed in flow cytometry staining buffer (PBS supplemented with 1% bovine serum albumin ...
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bioRxiv - Immunology 2024Quote: ... Mouse CD4+CD62L+ naïve T cells were isolated using a MojoSort Mouse CD4 T Cell Isolation Kit (BioLegend) on an autoMACS Pro Separator (Miltenyi Biotec) ...
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bioRxiv - Immunology 2024Quote: ... OVA-specific CD4+ T cells were purified from the spleen and lymph nodes of OTII TCR transgenic mice using the MojoSortTM Mouse CD4 Naïve T cell isolation Kit according to the manufacturer’s instructions (Biolegend). Cells were then stained with the CellTraceTM Violet Cell proliferation kit (ThermoFisher ...
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bioRxiv - Immunology 2024Quote: ... Concentrations of cytokines were determined using the following kits according to the manufacturer’s instructions: IL-12p70 (Biolegend, 431704), TNF (Biolegend ...
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bioRxiv - Immunology 2024Quote: Foxp3-YFP+ cells were FACS sorted from the spleens of 3-week-old Gsk3a/bΔTreg and WTΔTreg mice following CD4+ T cell enrichment (MojoSort Mouse CD4+ T cell Isolation Kit, Biolegend) by negative selection ...
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bioRxiv - Immunology 2024Quote: ... and mouse CD4+ and CD8+ T cells were isolated using MojoSort Mouse CD4 T Cell Isolation Kit (BioLegend) and MojoSort Mouse CD8 T Cell Isolation Kit (BioLegend) ...
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bioRxiv - Cell Biology 2021Quote: Detection of apoptotic cells was done by using FITC-Annexin V Apoptosis Detection Kit with 7-AAD (Biolegend#640922). Briefly ...
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bioRxiv - Genomics 2021Quote: ... for RNA library construction while modifications for CITE-seq ADT and cell hashing HTO library preparation were performed according to the TotalSeq™-A Antibodies and Cell Hashing with 10x Single Cell 3’ Reagent Kit v3 or v3.1 single index protocol (BioLegend). The modifications include the addition of ADT and HTO additive primers to a final concentration of 0.2 uM in the cDNA amplification reaction (step 2.2) ...
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bioRxiv - Immunology 2021Quote: ... The cells were washed with 1xPBS and fixed using buffer of the True-Nuclear Transcription Factor Staining kit (Biolegend). After fixing ...
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bioRxiv - Cell Biology 2021Quote: ... CD4+ T cells were then isolated by negative selection using the MojoSort Mouse CD4 T Cell Isolation Kit (Biolegend). Isolated CD4+ T cells were seeded at 1×106/mL in RPMI supplemented with 10% FBS ...
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bioRxiv - Immunology 2022Quote: ... Brefeldin A solution (420601) and CFSE Cell Division Tracker Kit (423801) were purchased from Biolegend (San Diego, CA, USA). PE anti-mouse IL-4 (12-7041-81) ...
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Nanoparticle-delivered TLR4 and RIG-I agonists enhance immune response to SARS-CoV-2 subunit vaccinebioRxiv - Immunology 2022Quote: Both FLT3L and GM-CSF BMDCs were stained with 1:1000 Zombie Green Fixable Viability Kit (BioLegend, Cat# 423111) and were blocked with anti-mouse CD16/32 (BioLegend ...
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bioRxiv - Immunology 2022Quote: ... The percentage of apoptotic cells were then determined using the FITC Annexin V apoptosis detection kit with PI (BioLegend) and flow cytometry as per manufacturer’s instructions.
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bioRxiv - Immunology 2022Quote: CD4+ T cells were isolated from naïve gender- and age-matched WT and CD6Δd3 splenocytes by negative selection using magnetic beads (Mojo sort kit, BioLegend). Isolated cells were activated with 3 μg plate-bound anti-mouse CD3 (145-2C11 ...
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bioRxiv - Genetics 2022Quote: ... two-week C57BL/6 c-Kit+ bone marrow cells were cultured in media with recombinant mouse interferon alpha (Biolegend) as indicated with media changes every 2-3 days ...
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bioRxiv - Cancer Biology 2022Quote: ... and lineage-negative cells (Lin-) were analyzed for c-Kit (clone 2B8; eBioscience) and Sca-1 (clone D7; BioLegend) expression to discriminate Kit+ Sca-1- (KL ...
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bioRxiv - Cancer Biology 2020Quote: ... Cells were prepared for flow cytometry two days after media change using the FITC Annexin V kit (Biolegend, 640906). Quantification was performed using a BD FACSCanto 10 flow cytometer.
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bioRxiv - Neuroscience 2020Quote: ... cells were harvested and stained with AV and PI according to manufacturer’s manual (FITC Annexin V Apoptosis Detection Kit with PI; Biolegend). Stained cells were immediately analyzed by flow cytometry (MACSQuant ...