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Citations for BioLegend :
3801 - 3850 of 3905 citations for Human Bcl2 Associated Death Promoter BAD ELISA Kit since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2023Quote: ... neutrophils were isolated by negative sorting by magnetic selection using the Mojosort neutrophil isolation kit according to the suggested protocol (Cat# 480058-BioLegend). Purity was checked by flow cytometry using CD11b and Ly6G surface staining ...
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bioRxiv - Cancer Biology 2023Quote: ... Viability of BMNC (0.25 x106) was assessed by Annexin V and 7-AAD staining (APC Annexin V Apoptosis Detection Kit 7-AAD, Biolegend) using FCM.
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bioRxiv - Physiology 2023Quote: ... Serum samples were prepared for Legendplex assays by diluting 1:50000 for the detection of immunoglobulin isotypes using the mouse immunoglobulin isotyping kit (BioLegend). The assay was performed in a V-bottom plate and data was acquired on a BD Fortessa X-30 cytometer (BD Biosciences) ...
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bioRxiv - Genetics 2023Quote: ... Lin-Sca-1+ c-Kit+ Flt3-CD150+ CD48-on a FACSAria into StemSpan SFEM II media with SCF (100 ng/ml, BioLegend), TPO (50 ng/ml ...
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bioRxiv - Cancer Biology 2023Quote: ... Cells were washed twice using ice-cold PBS and stained using Zombie Aqua™ Fixable Viability Kit (Cat. 423101, Biolegend). Cells were washed and resuspended in FACS buffer (1% BSA ...
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bioRxiv - Cancer Biology 2024Quote: ... T cells were isolated from the spleen and lymph nodes of FVB/N mice using the MojoSort Mouse CD8 T cell isolation kit (BioLegend) as per the manufacturer’s protocol for negative selection strategy ...
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bioRxiv - Microbiology 2024Quote: Cytokine concentrations in supernatants from cultured BMDMΦ or sera of animals were assessed using the Legendplex mouse inflammation kit (BioLegend) following the manufacturer’s instructions as described previously (18 ...
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bioRxiv - Immunology 2024Quote: ... Apoptosis analysis was done with the APC Annexin V (1:50) apoptosis detection kit with PI (1:200) in Annexin V Binding Buffer (Biolegend).
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bioRxiv - Cell Biology 2024Quote: ... BrdU was added to cell cultures 30 minutes prior to collection and fixation using the BD cytofix/cytoperm kit and staining with an anti-BrdU-APC (Biolegend) antibody ...
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bioRxiv - Microbiology 2024Quote: ... washed twice with cold FACS buffer (3%FCS/PBS) and analyzed for apoptosis or necroptosis using the APC Annexin V Apoptosis Detection Kit with propidium iodide (PI) (Biolegend) following the manufacturer’s instructions ...
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bioRxiv - Immunology 2024Quote: ... The Mojosort monocyte isolation kit was used to isolate monocytes by negative selection according to the manufacture’s protocol (Biolegend, #480059) and cells were resuspended in RPMI supplemented to with 100ng/mL M-CSF (Peprotech ...
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bioRxiv - Immunology 2024Quote: ... naïve CD4 and CD8 T cells were isolated from the spleen and peripheral LNs of C57BL/6 mice using the MojoSort™ Mouse CD4 or CD8 Naïve T Cell Isolation Kit from BioLegend and irradiated with 254 nm-UV light (600 mJ/cm2 using a Stratalinker 2400) ...
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bioRxiv - Immunology 2024Quote: ... and the naïve T cells were harvested using a MojoSort™ mouse CD8+ T cell isolation kit (#480008, Biolegend, US) following the manufacturer’s instructions ...
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bioRxiv - Immunology 2024Quote: CD8+ T cells were magnetically purified from the spleens of indicated donor mice by negative selection using MojoSort Mouse CD8 T cell Isolation kit (Biolegend) to >95% purity ...
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bioRxiv - Immunology 2024Quote: ... filtered through 100 μm cell strainer and enriched for CD45- cells by removing the CD45+ cells using the MojoSort mouse CD45 nanobeads kit (Biolegend). Cells were then stained with Live-Dead Blue ...
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bioRxiv - Immunology 2024Quote: ... T cells were isolated from splenocytes of BALB/c mice using a MojoSort mouse CD3 T cell isolation kit (Biolegend) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2024Quote: CD8+ T cells were negatively isolated from spleen and peripheral LNs of donor mice using the MojosortTM Mouse CD8+ T cell Isolation Kit (BioLegend). T cell purity was confirmed to be > 95% by flow cytometry ...
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bioRxiv - Immunology 2024Quote: CD4+YFP+ Treg cells from Foxp3cre and Foxp3cre Cxxc1fl/fl mice were enriched using the Mouse CD4 T Cell Isolation Kit (480005; Biolegend) and then sorted using the BD Aria II flow cytometer ...
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bioRxiv - Molecular Biology 2020Quote: ... and LCL A382T TDP-43 mutated patients (LCL-TDP382) were incubated with a vitality dye for 15 minutes (Zombie Violet™ Fixable Viability Kit, BioLegend). Then ...
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bioRxiv - Molecular Biology 2020Quote: ... together with the trypsinized cells were harvested into tubes and samples were prepared on ice for flow cytometry using the Pacific Blue Annexin V Apoptosis Detection Kit with 7-AAD (Biolegend: 640926) according to the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2020Quote: Freshly isolated CD3+ T cells were labeled using CFSE working solution (5 μM) using the CFSE Division Tracker Kit (Biolegend, USA). T cells were incubated for 20 min at 37 ◻ and quenched staining with culture medium ...
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bioRxiv - Immunology 2021Quote: Naïve CD8+ T cells (OT-1 or Pmel-1) were isolated from pooled spleen and lymph nodes using MojoSort™ mouse CD8 T cell isolation kit (Biolegend) following manufacturer’s instruction ...
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bioRxiv - Cancer Biology 2020Quote: ... fixed and stained for intracellular Ki67 following BD Pharmingen’s procedure for FITC BrdU Flow Kit (Cat# 559619) with two modifications: DNase treatment step was omitted and Ki67-AlexaFluor488 (Biolegend #350507) was used instead of anti-BrdU FITC.
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bioRxiv - Microbiology 2022Quote: Lung tissue leukocyte samples were subjected to viability staining and blocking of Fc-receptors using LIVE/DEAD™ Fixable Blue Dead cell stain kit (life technologies) and an anti-CD16/32 antibody (clone 93, BioLegend). Cells were then washed and incubated with a staining mix containing antibodies against the following murine antigens ...
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bioRxiv - Immunology 2022Quote: ... and then stained at room temperature for 15 min with a 1:200 dilution of the LIVE/DEAD Zombie Aqua™ Fixable Viability Kit (Biolegend) reagent to exclude dead cells ...
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bioRxiv - Microbiology 2020Quote: Levels of cytokines and soluble mediators present in plasma of infected mice were quantified using LEGENDplex™ Mouse Inflammatory Panel kit (Biolegend) according to the manufacturer’s protocol ...
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bioRxiv - Immunology 2020Quote: ... DCs were isolated from the resulted mononuclear cells using a MojoSort™ Mouse Pan Dendritic Cell Isolation Kit (BioLegend cat. 480097). Single molecule FISH experiments were carried out using an RNAscope® Multiplex Fluorescent Assay v2 along with an RNAscope® Probe-Mm-Krt14-C2 (ACD cat ...
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bioRxiv - Immunology 2022Quote: ... cells were washed twice with PBS buffer and stained with a kit of Zombie Aqua™ Fixable Viability (Biolegend, cat.423101) for 20 min at RT ...
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bioRxiv - Cancer Biology 2022Quote: ... the cells were stained with Annexin V/PI using the Apoptosis Detection Kit according to the manufacturer’s protocol (BioLegend, Cat# 640945). Cells were acquired on a BD LSR Fortessa ...
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bioRxiv - Immunology 2022Quote: ... True-Nuclear™ One Step Staining Mouse Treg Flow™ Kit (FOXP3 (clone MF-14) with CD25 and CD4) was used with FOXP3 Fix/Perm Buffer from BioLegend per manufacturer’s protocol ...
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bioRxiv - Immunology 2022Quote: ... Primary antibodies, anti-PDGFRa (AB Online, # ABIN726620), anti-Kitl (R&D, #AB- 455-NA) and anti-c-Kit (Biolegend, clone 2B8) were then stained at a 1:200 dilution for 72hrs ...
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bioRxiv - Cancer Biology 2022Quote: Oligo-labeled antibody staining was done on both melanoblasts and melanom cells using the TotalSeq™-A Antibodies and Cell Hashing with 10× Single Cell 3′ Reagent Kit (Biolegend). The HashTag Oligonucleotidess used for the analysis are mHTO01 ...
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Autocrine Sfrp1 inhibits lung fibroblast invasion during transition to injury induced myofibroblastsbioRxiv - Cell Biology 2022Quote: ... an aliquot of 3×105 cells in 1ml MACS buffer was incubated at RT for 15 minutes with 1:500 dilution of Zombie UV™ Fixable Viability Kit (Biolegend). Cells were stained with 1 µl of anti-GFP antibody (Santa Cruz ...
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bioRxiv - Immunology 2024Quote: Cells were stained with 20 µL of surface antibody cocktail with Zombie NIR fixable viability kit (1:400-1000, Biolegend, 423105) in PBS for 20 minutes on ice ...
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bioRxiv - Immunology 2023Quote: ... CD4 T cells were isolated from the spleen and LNs of indicated naïve OT-II strains with Mouse CD4 T Cell Isolation Kit (STEMCELL Technologies 19852 or BioLegend 480033) and labeled with 1.0 µM CFSE (Thermo Fisher ...
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bioRxiv - Cancer Biology 2024Quote: Apoptotic and necrotic cell analysis was performed using Pacific Blue™ Annexin V Apoptosis Detection Kit with 7-AAD (BioLegend, #640926) as per manufacturer’s instructions ...
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bioRxiv - Neuroscience 2023Quote: ... libraries were prepared according to the Chromium Single Cell 3ʹ with Feature Barcoding technology for Cell Surface Protein Reagent Kit v3 protocol (10X Genomics) with modifications for incorporation of TotalSeq™-A HTO antibodies (clones M1/42; 30-F11, BioLegend). Briefly ...
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bioRxiv - Immunology 2023Quote: ... Cells were incubated in 200 μl FACS buffer containing 1 μl Zombie NIR fixable dye for 30 min to determine cell viability (Zombie Green™ Fixable Viability Kit, BioLegend). Cells were fixed for 15 min in 200 μl PFA 2 % ...
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bioRxiv - Microbiology 2023Quote: ... cells were incubated in 100 µl PBS 1X containing 1.5 μL Zombie NIR Fixable Viability kit (BioLegend, San Diego, CA, USA) at 4°C for 30 min ...
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bioRxiv - Immunology 2023Quote: ... Ex vivo CD8+ T cells or in vitro stimulated (IVS) cultures were labeled with the Zombie Aqua™ Fixable Viability Kit (BioLegend) to exclude dead cells in all flow cytometry experiments ...
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bioRxiv - Cell Biology 2022Quote: ... Twenty four hours after irradiation cells were trypsinized and stained using the FITC Annexin V Apoptosis Detection Kit with PI (Biolegend, CA) following the manufacturer’s instructions ...
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bioRxiv - Immunology 2023Quote: ... resuspended in 1 ml of PBS containing 1 µl live/dead stain (Zombie UV Fixable Viability kit (Biolegend, cat. no. 423107)) and incubated on ice for 30 min ...
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bioRxiv - Developmental Biology 2023Quote: The flow cytometry staining for necrosis and apoptosis was was performed with the FITC Annexin V Apoptosis Detection Kit with 7-AAD (Biolegend, 640922). Once the cells were incubated in 10 µM of EdU ...
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bioRxiv - Immunology 2023Quote: ... CD3+ T cells were isolated from spleens of 6-to-8-week-old OT-II mice by MojoSort mouse CD3+ T cell isolation kit (Biolegend 480024). Stimulated FLS were washed with PBS and co-cultured with CD3+ T cells at a concentration of 5-10×105 cells/mL in 10% RPMI for 5 days with either the cognate OVA323-339 antigen (2μg/mL ...
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bioRxiv - Immunology 2023Quote: ... CD3+ CAR-T cells were then purified from lymphocytes using MojoSort™ Mouse CD3 T Cell Isolation Kit according to the manufacturer’s instructions (BioLegend, 480031). CAR-T cells were then activated for 2 days at 1 × 106 cells per 24-well flat-bottomed plates with an equivalent number of CD3/CD28 washed Dynabeads (Gibco ...
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bioRxiv - Immunology 2023Quote: Proliferation of blood ASC and BM LLPC was evaluated on the basis of bromodeoxyuridine (BrdU) incorporation using a phase-flow BrdU commercial kit (BioLegend, Inc.) in accordance with the manufacturer’s recommendations ...
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bioRxiv - Immunology 2023Quote: ... For adoptive transfer and co-culture experiments naïve OT-II cells were magnetically enriched with MojoSort™ Mouse CD4 Naïve T Cell Isolation Kit (BioLegend Cat: 480040) according to manufacturer’s protocol ...
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bioRxiv - Immunology 2024Quote: ... Naive CD4+ cells were isolated by negative selection using the MojoSort CD4+ naïve T-cell isolation kit from Biolegend (Catalog 480040) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2024Quote: ... CD4+ T cells were negatively selected from the lung single-cell suspensions utilizing the MojoSort™ Mouse CD4 T Cell Isolation Kit (BioLegend) in alignment with the manufacturer’s guidelines.
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bioRxiv - Cell Biology 2024Quote: hiPSC-CMs were dissociated into single cells with 0.05% trypsin-EDTA and stained with the fixable Zombie NIR™ Viability Kit (BioLegend®) according to the manufacturer’s protocol ...