Labshake search
Citations for BioLegend :
3201 - 3250 of 10000+ citations for Rabbit Anti Human IgG Fc Biotin since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Immunology 2019Quote: ... to block non-specific binding of antibodies to Fc Receptors and then incubated at 4°C for 30 minutes with Fixable Viability Staining e780 and fluorochrome/biotin conjugated antibodies (purchased from BD Bioscience, Biolegend, Thermofisher Scientific or Miltenyi biotec ...
-
bioRxiv - Cell Biology 2022Quote: ... Erythroid precursors were isolated using negative selection by lineage-depletion with biotin- conjugated antibodies and MojoSort streptavidin-conjugated magnetic nanobeads (Biolegend): Anti-mouse CD3e (clone 145-2C11) ...
-
bioRxiv - Immunology 2020Quote: ... ABCs were isolated from purified total B cells by CD11c positive selection using a biotin conjugated CD11c antibody (301612, Biolegend) and anti-biotin magnetic beads ...
-
bioRxiv - Immunology 2020Quote: ... single-cell IEL suspension was incubated with staining buffer (PBS containing 2% FBS with 2 mM EDTA) containing a mixture of biotin-conjugated mAbs against CD4 (RM4-5; BioLegend), CD19 (6D5 ...
-
bioRxiv - Immunology 2022Quote: ... cells were resuspended in FACS buffer (1% BSA in PBS) at a concentration of 108 cells/mL and 1 μL negative CD8+ T cell biotin-antibody cocktail (Biolegend)/million cells was added and allowed to incubate for 15 min on ice ...
-
bioRxiv - Bioengineering 2024Quote: ... Biotinylated rasburicase was reacted for 20 min on ice with 4:1 molar ratio of biotin to streptavidin-conjugated PE or streptavidin-conjugated APC (Biolegend). Streptavidin-conjugated FITC (BioLegend ...
-
bioRxiv - Immunology 2023Quote: ... samples from animals that were vaccinated with the recombinant GPCysR4-I53-50A.30 To exclude I53-50A-binding B cells we included a SARS-CoV-1 Spike probe containing I53-50A-biotin which was labeled with streptavidin AF647 and streptavidin BV421 (both Biolegend). After a 2 day culture with interleukin (IL)-21 and irradiated mouse fibroblast (L cells ...
-
bioRxiv - Immunology 2023Quote: ... CD8 (biotin, clone: 53-6.7), CD205 (clone: NLDC-145) and TNF-α(PE, clone: MP6-XT22) were purchased from BioLegend. The CD18-reactive mAb M18/2 was purchased from Biozym ...
-
bioRxiv - Cancer Biology 2023Quote: ... non-natural killer cells were depleted by incubating the patient’s whole PBMCs sample with the biotin antibody cocktail (BioLegend 480053) followed by incubation with magnetic Streptavidin Nanobeads ...
-
bioRxiv - Bioengineering 2023Quote: ... Biotinylated uricase was reacted for 20 min on ice with 4:1 molar ratio of biotin to streptavidin-conjugated PE or streptavidin-conjugated APC (Biolegend). Streptavidin-conjugated FITC (BioLegend ...
-
bioRxiv - Immunology 2024Quote: ... were stained on ice for 25 minutes with the following panel of lineage-specific antibodies: Biotin-labeled TER-119 (TER-119, BioLegend), Biotin-labeled GR-1 (RB6-8C5 ...
-
bioRxiv - Neuroscience 2020Quote: Free floating 30 μm sagittal brain sections were treated for antigen retrieval using sodium citrate buffer and subsequently stained using primary antibodies for Iba1 (1:250, rabbit, Wako) and anti-6E10 (1:1000, Biolegend), and the corresponding secondary antibodies and nuclear counterstain using DAPI ...
-
bioRxiv - Developmental Biology 2022Quote: ... MGs were localized using the mT/mG reporter or stained using a rabbit anti-K14 polyclonal antibody (1:500, 905301, BioLegend). Nuclei were counterstained with DAPI ...
-
bioRxiv - Immunology 2022Quote: ... at a 1:200 dilution for 30 mins at room temperature and was detected with anti-rabbit BV421 (Dnk, BioLegend). Flow was performed on Cytek Aurora (Cytek ...
-
bioRxiv - Microbiology 2019Quote: ... The membrane was incubated with anti-ShyA polyclonal antibody (1:5,000, produced by Pocono Rabbit Farm & Laboratory, PA) or monoclonal anti-αRpol antibody (1:15,000, BioLegend cat# 663104) for two hours and then washed with TBST (20 mM Tris-HCl (pH7.8) ...
-
bioRxiv - Neuroscience 2022Quote: ... rabbit) at 1:100 dilution (for immunodepletion) or 3 μg/ml of each 4G8 and 6E10 anti-Aβ antibodies (Biolegend) (for brain-derived Aβ enrichment ...
-
bioRxiv - Immunology 2023Quote: ... and goat anti-rabbit Alexa Fluor Plus 647-coupled secondary antibody (1:500) in Intracellular Staining Permeabilization Wash Buffer (Biolegend). The fraction of cells positive for cleaved caspase-3 was measured with a BD LSRFortessa SORP flow cytometer ...
-
bioRxiv - Cancer Biology 2023Quote: ... mice were injected intraperitoneally with three hundred micrograms of Ultra-LEAF purified anti-Asialo GM1 rabbit polyclonal antibody (clone Poly21460, BioLegend) every four days for the duration of the experiment ...
-
bioRxiv - Immunology 2019Quote: ... human T cells were directly enriched from PBMC by negative selection using MojoSort™ Human CD3 T Cell Isolation Kit (Biolegend, CA) following the manufacturer’s instructions ...
-
bioRxiv - Microbiology 2020Quote: ... approximately 2-10 million total human lung cells were resuspended in MACS buffer and blocked with Human TruStain FcX (Biolegend cat# 422032) for 15min at 4°C followed by HTII-280 antibody (Terrace Biotech ...
-
bioRxiv - Bioengineering 2024Quote: ... The levels of cytokines IL-2 and IFN-γ were quantified using ELISA MAX Deluxe Set Human IL-2 and ELISA MAX Deluxe Set Human IFN-γ (BioLegend).
-
bioRxiv - Cell Biology 2020Quote: ... Cells were incubated with Live/Dead Yellow (1:1000) followed by Fc block (1:200; BioLegend, San Diego, CA), and stained with antibody cocktails for 30 minutes on ice ...
-
bioRxiv - Immunology 2021Quote: ... Cells were incubated at 4°C for 15 minutes with an Fc receptor blocker (BioLegend TruStain FcX, cat # 422302) according to manufacturer instructions before cocktails of surface antibodies were added for 30 minutes at 4°C ...
-
bioRxiv - Cancer Biology 2021Quote: ... Red blood cells were lysed (15mM NH4Cl + 10mM KHCO3 for 5 min at R.T.) and CD16/32 antibody was used to block the endogenous FC (#101301, Biolegend). Cells were analysed using a BD FACS LSR Fortessa ...
-
bioRxiv - Immunology 2022Quote: ... mouse TNF-α (clone MP6-XT22), mouse CD8α (clone 53-6.7), and mouse CD16/32 (Fc block, clone 93) were purchased from Biolegend. Other reagents used include Zombie Aqua Viability Dye (Biolegend) ...
-
bioRxiv - Immunology 2020Quote: ... Blocking of Fc receptors in T2 cells was performed prior to staining using the TruStain FcX reagent (BioLegend, #422301). Staining with peptide-MHC dextramers was performed for 10 min at room temperature (RT) ...
-
bioRxiv - Immunology 2022Quote: ... Samples were washed in PBS and were incubated at 4°C with CD16/32 Fc block (cat#101302, BioLegend) in PBS for 10 minutes before staining with antibodies (Supplementary Table 1 ...
-
bioRxiv - Immunology 2022Quote: ... before leukocytes were separated using 30 and 70% Percoll gradients and subsequently stained for 30 minutes in FACS buffer (PBS/2.5%FCS/10mM EDTA/0.01% NaN3) with the following primary antibodies: CD45-PacificBlue (Biolegend, 103126), CD19-biotin (Biolegend ...
-
bioRxiv - Neuroscience 2024Quote: ... Cells were then blocked on ice with PBS + 7.5% FCS for 15 minutes before staining with antibody cocktails (see key resource table) containing TruStain Monocyte Blocker (Biolegend) in FACS buffer for 45 minutes at 4°C ...
-
bioRxiv - Cell Biology 2023Quote: ... approximately 200,000 cells per sample were resuspended in staining buffer (PBS, 2% BSA, and 0.01% Tween) and incubated for 10 minutes with Fc block (TruStain FcX (BioLegend) and FcR blocking reagent (Miltenyi) ...
-
bioRxiv - Immunology 2023Quote: ... cells were stained for sorting in PBS containing 2% FCS at 4 °C with the following antibodies: CD271-PE (1:200; ME20.4, BioLegend), CD235a-PECy7 (1:20 ...
-
bioRxiv - Immunology 2023Quote: ... Cells were washed with LunaStain cell staining buffer (Polaris Biology, China) and first stained with Fc block (Biolegend, USA) for 10 min at room temperature ...
-
bioRxiv - Immunology 2023Quote: ... Cells were subsequently resuspended in staining buffer (PBS with 0.5% bovine serum albumin and 2 mM EDTA) and Fc-blocked using TruStain FcX reagent (BioLegend). Cells were then stained with the following fluorophore-conjugated anti-mouse antibodies for 30 min at 4°C ...
-
bioRxiv - Developmental Biology 2023Quote: ... then resuspended in ice-cold MACS buffer (DPBS + 0.5% BSA + 2mM EDTA) and incubated with Fc Block (Biolegend 156603) for 5 min at 4°C ...
-
bioRxiv - Cell Biology 2022Quote: ... one million cells were first resuspended in 100 μL buffer containing 5 μL Fc Receptor Blocking Solution (Biolegend, #422302) and incubated for 15 minutes ...
-
bioRxiv - Immunology 2024Quote: ... and single cell preparations were obtained by passing the cell mixture through a PluriStrainer (500 – 70µM cell strainers; 43-50500, PluriSelect) before blocking with Fc block for 10 min (422302, BioLegend). CD45+ cells were enriched using anti-CD45 magnetic MicroBeads and LS columns (130-045-801 and 130-042-401 ...
-
bioRxiv - Immunology 2024Quote: ... HVEM binding to BTLA was assessed by incubating cells with the HVEM-Fc recombinant protein (20 µg/ml; Biolegend) followed by a staining with an Alexa488 conjugated-anti-IgG1 secondary Ab (clone HP6069 ...
-
bioRxiv - Immunology 2022Quote: PBMCs were firstly incubated with Human TruStain FcX (Biolegend) at 4 °C for 30 min and then stained with biotin-conjugated SARS-CoV-2 RBD protein (Sino Biological ...
-
bioRxiv - Neuroscience 2019Quote: ... iMGLs were stimulated with human BMP9 (20ng/ml; Biolegend), Pam3csk (100 ng/ml ...
-
bioRxiv - Immunology 2021Quote: ... the cells were supplemented with Human TruStain FcX (Biolegend) to reduce non-specific antibody staining and 5uM Rhodium-103 (Fluidigm ...
-
bioRxiv - Genetics 2020Quote: ... cells were blocked with Human TruStain FcX (Biolegend 422302) and stained at 4°C in a final volume of 50µL with the following antibodies ...
-
bioRxiv - Microbiology 2020Quote: ... as well as human CD8 were purchased from Biolegend.
-
bioRxiv - Cell Biology 2021Quote: ... and blocked with Human TruStain FcX blocking antibody (Biolegend). CD45-PE [clone HI30] ...
-
bioRxiv - Microbiology 2022Quote: ... ELISA MAX Deluxe Set Human IL-1a (Biolegend, 445804), and ELISA MAX Deluxe Set Human IL-1b (Biolegend ...
-
bioRxiv - Cancer Biology 2022Quote: ... samples were stained with Human TrueStain FcX (Biolegend; 422303) and Zombie Dye Violet Blue (Biolgend ...
-
bioRxiv - Molecular Biology 2022Quote: ... The LEGEND MAX Human IL-1α ELISA Kit (Biolegend) was used to assay for IL-1α concentration ...
-
bioRxiv - Immunology 2022Quote: ... and pre-incubated with Human TruStain FcX blocker (BioLegend) for 5 min at RT ...
-
bioRxiv - Immunology 2021Quote: ... PBMCs were first resuspended with Human TruStain Fcx (Biolegend) for 10 minutes at room temperature and then stained with the following antibodies in FACS buffer (PBS+ 2% fetal bovine serum) ...
-
bioRxiv - Cell Biology 2019Quote: ... Human IL-2 was quantified by ELISA (BioLegend, # 431804).
-
bioRxiv - Immunology 2020Quote: ... and blocking was performed using Human TruStain FcX (BioLegend). CD3+CD4+CD25+CD127lo/- nTregs and CD3+CD4+CD25- Tconvs were isolated on the Astrios (Beckman Coulter ...