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Citations for BioLegend :
3401 - 3450 of 10000+ citations for Rabbit Anti Human IgG Fc Biotin since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2022Quote: ... α-human HLA-A,B,C (W6/32, conjugated to APC, Biolegend). Phytohemagglutinin PHA-L (L4144 Sigma ...
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bioRxiv - Immunology 2022Quote: ... cells were analyzed using Biolegend’s LEGENDScreenTM Human PE Kit (Biolegend, 700007). Subjects were barcoded with combinations of CD45 antibodies conjugated to three fluorophores ...
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Orchestration of alternative splicing regulates bone marrow mesenchymal stem cells fate during agingbioRxiv - Cell Biology 2022Quote: ... and PE-conjugated antibodies that recognized human Stro-1 (BioLegend, 340106), CD45 (BioLegend ...
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bioRxiv - Immunology 2021Quote: ... we used human RP10 media with combinations of rIL-6 (BioLegend), rIL-12 ...
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bioRxiv - Immunology 2022Quote: ... and recombinant human transforming growth factor beta-1 (TGF-β1; Biolegend) were used at final concentrations of 10 ng/mL ...
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bioRxiv - Immunology 2023Quote: ... we used the LEGENDplexTM Human CD8/NK Panel (13-plex) (Biolegend) following the manufacturer’s instructions ...
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bioRxiv - Genomics 2024Quote: ... then stained using a custom TotalSeq-A Universal Human Panel (BioLegend) for 30 minutes ...
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bioRxiv - Biochemistry 2024Quote: ... the BM was blocked with Human TruStain FcX™ (Biolegend, #422302) and TruStain fcX™ (anti-mouse CD16/32 ...
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bioRxiv - Immunology 2023Quote: ... Cells were blocked with 10ul of human FcX block (BioLegend, 422302) for 10 min at room temperature ...
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bioRxiv - Immunology 2023Quote: ... and 1 U/mL of recombinant human IL-2 (BioLegend, #589102) in 200 μL of complete culture medium (see above for media components) ...
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bioRxiv - Microbiology 2023Quote: Cytokines were measured using the LEGENDplex human antivirus response panel (BioLegend). The kit was used according to the manufacturer’s manual with an additional fixing step ...
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bioRxiv - Immunology 2023Quote: ... were coated with 1.54 μg/mL human recombinant VCAM-1 (Biolegend) and/or 1.14 μg/mL human recombinant ICAM-1 (R&D system ...
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bioRxiv - Cell Biology 2023Quote: ... and human CD81(Biolegend, Cat. No. 349502, San Diego, CA, USA) (exomap1::Camk2a-Cre mouse and control exomap1+/ mouse) ...
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bioRxiv - Cell Biology 2023Quote: ... and human CD81 (BioLegend, Cat. No. 349502, San Diego, CA, USA). All antibodies were fluorescently labeled as described previously at a molar ratio of ∼1 (70)) ...
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bioRxiv - Bioengineering 2023Quote: ... and 500 IU/ml of recombinant human IL-2 (Biolegend, 589104). Stimulated T cells were co-transduced via spinoculation at 931xg ...
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Cell therapy with IL-10-producing group 2 innate lymphoid cells suppresses Graft-versus-Host diseasebioRxiv - Immunology 2023Quote: ... or the 13-plex LegendPlex Human Proinflammatory Chemokine Panel 1(BioLegend) on thawed supernatant samples per manufacturer’s instructions.
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bioRxiv - Cell Biology 2022Quote: ... cells were blocked with human TruStain FcR Blocking Reagent (Biolegend, USA) and True-Stain Monocyte Blocker (Biolegend ...
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bioRxiv - Genomics 2023Quote: ... and blocked with 5 µL Human TruStain FcX™ (422301, Biolegend) for 10 minutes at 4 °C ...
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bioRxiv - Immunology 2023Quote: ... The cells were first resuspended in Human TruStain FcX (Biolegend; 422302) in 25 µl FACS buffer for 15 minutes on ice ...
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bioRxiv - Cell Biology 2023Quote: ... HeLa cells were treated with TNF-α recombinant human protein (Biolegend) at a concentration of 75 ng/ml for 24 h in combination with cycloheximide (CHX ...
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bioRxiv - Immunology 2023Quote: ... or human TruStain FcX CD16/32/64 antibodies (BioLegend; cat # 422302) to block Fc receptors ...
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bioRxiv - Cell Biology 2023Quote: ... supplemented with 1000 U/ml of human recombinant IFN-γ (BioLegend), 20 ng/ml of EGF (Sigma ...
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bioRxiv - Immunology 2023Quote: ... then incubated with fluorophore-labeled antibodies to human EpCAM (BioLegend 118213), NGFR (Abcam ab52987) ...
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bioRxiv - Immunology 2024Quote: ... Cells were subsequently incubated with a cocktail containing Human TruStainFcX (Biolegend) and PBS-57/CD1d-tetramers (NIH Tetramer Facility ...
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bioRxiv - Immunology 2024Quote: ... CD14+ monocytes were magnetically labeled using MojoSort Human CD14 Nanobeads (Biolegend) and subsequently separated on LS columns using a QuadroMACS™ Separator (Miltenyi Biotec) ...
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bioRxiv - Immunology 2024Quote: ... cells were analyzed using Biolegend’s LEGENDScreenTM Human PE Kit (Biolegend, 700007). Seven subjects were incubated with IL-15 and IL-21 (as above ...
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bioRxiv - Immunology 2022Quote: ... in FACS buffer for 30 minutes at 4°C followed by 2 wash steps with FC stain buffer and resuspended in Fluorofix (BioLegend, cat. 422101) for 30 minutes at 4°C ...
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bioRxiv - Immunology 2023Quote: ... Cells were pelleted down at 1,500 rpm at 4°C for 5 min and cell pellets were resuspended in 100 uL FACS buffer (PBS + 4% FBS +0.5 mM EDTA) containing Fc block (BioLegend, 101302, 1:200). After 15 min incubation at 4°C ...
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bioRxiv - Immunology 2023Quote: ... Cells were suspended in PBS + 2% HI-FBS + 2mM EDTA in the presence of Fc receptor blocking antibody (BioLegend, 101302, 1:400) and stained with antibodies at a 1:400 dilution against the following mouse markers ...
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bioRxiv - Neuroscience 2022Quote: ... was added to differentiation media and 10µg/ml of control IgG (Biolegend 403501) was added to the differentiation and proliferation control samples ...
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bioRxiv - Immunology 2021Quote: ... Ultra-LEAF™ Purified Armenian Hamster IgG Isotype Ctrl (clone HTK888, 400969, BioLegend), BIO 5192 (R&D systems) ...
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bioRxiv - Immunology 2024Quote: ... or a control isotype-matched antibody IgG (clone Poly4053) (BioLegend, R&D systems) were added at the primary phase (day 0 and 7 ...
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bioRxiv - Neuroscience 2021Quote: ... FCS files were evaluated with the LEGENDplex™ Data Analysis Software Suite using the Qognit cloud base platform (Biolegend, San Diego, CA, USA). A five-parameter logistic curve-fitting method was used to calculate concentrations from Median Fluorescence Intensity and normalized to the amount of protein in each sample ...
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bioRxiv - Microbiology 2021Quote: ... approximately 200,000 cells per sample were resuspended in staining buffer (PBS, 2% BSA, 0.01% Tween) and incubated for 10 minutes with Fc block (TruStain FcX, Biolegend; FcR blocking reagent, Miltenyi). Cells were then incubated with barcoded hashing antibodies for 30 min at 4 °C ...
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bioRxiv - Developmental Biology 2022Quote: Single-cell suspensions were obtained as described above and subsequently blocked for 5-minutes on ice with Fc block CD16/32 (Biolegend, #101302, 1:100), followed by the addition of antibodies for 30-minutes on ice ...
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bioRxiv - Immunology 2019Quote: ... cells were washed with 200 μl FACS Buffer and blocked in 100 μl Fc block (cat. no. 101319, TruStain FcX, clone 93, BioLegend, diluted 1:100) in FACS Buffer for 10 min on ice and washed ...
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bioRxiv - Cancer Biology 2024Quote: ... Aliquots were thawed and incubated with the panel of antibodies following the blockade of non-specific binding of the immunoglobulin to the Fc receptors by TruStain FcX (BioLegend, San Diego, CA). LIVE/DEAD Fixable Near-IR Dead Cell Stain dye (Thermo Fisher Scientific ...
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bioRxiv - Immunology 2022Quote: ... Cells were centrifuged for 5 min at 500 x g and 4 °C and stained with Fc receptor blocker (TruStain fcX™, BioLegend, 1:100) and viability stain (Zombie Aqua™ Fixable Viability Kit ...
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bioRxiv - Immunology 2023Quote: ... tumor cell suspensions were incubated 10 min at RT with Fc block (Leico; C381) and a viability dye (Biolegend; Zombie Aqua (ref # 423102) or Zombie NIR (ref # 423106) ...
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bioRxiv - Immunology 2019Quote: ... The cells were washed and resuspended with Human TruStain FcX (Biolegend; 422302) for five minutes before adding the primary surface staining cocktail for 20m ...
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bioRxiv - Immunology 2019Quote: ... cells were pre-incubated with Human TruStain FcX™ (BioLegend, catalog 422301) to block Fc receptors ...
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bioRxiv - Biochemistry 2021Quote: ... The assay was performed using human IL-8 ELISA Max Deluxe (BioLegend) following manufacturer’s instruction ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... a final concentration of 5ng/ml of recombinant human TGFβ1 (BioLegend, #580702) was added to the culture media and treated for 24~48 hours ...
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bioRxiv - Cancer Biology 2022Quote: ... CD74 (mouse (151005) and human (326811)) from BioLegend (St. Diego, CA, USA), MHCII (47-5321-80 ...
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bioRxiv - Immunology 2022Quote: ... 100ng/ml of recombinant human (rh) IL-8 (Biolegend, San Diego, CA) were added to the apical side of the enteroid monolayers (76) ...
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bioRxiv - Cell Biology 2022Quote: LEGENDplexTM Human Adhesion Molecule Panel (13-plex) w/VbP LEGENDplex™ (Biolegend) and human inflammatory panel 1 (13-plex ...
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bioRxiv - Neuroscience 2022Quote: The LEGEND MAXtrademark Human α-Synuclein ELISA Kit (BioLegend; Cat. No. 844101) was kindly supplied by Biolegend ...
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bioRxiv - Microbiology 2022Quote: ... in the presence of 50 ng/mL recombinant human M-CSF (Biolegend) for 7 days ...
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bioRxiv - Immunology 2019Quote: ... and LEGENDplex Human Th Cytokine Panel (13-plex) kit (BioLegend, California/USA), according to the manufacturers’ instructions ...
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bioRxiv - Immunology 2019Quote: ... SEB (0.5 μg/mL) with human TNF cytokine (Biolegend, 125 ng/mL), or SEB (0.5 μg/mL ...