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Citations for BioLegend :
3051 - 3100 of 10000+ citations for Rabbit Anti Human IgG Fc Biotin since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2024Quote: ... cells were stained with the following antibody cocktail diluted in flow cytometry staining buffer: mouse anti-human CD3 BV750 (BioLegend Cat 344846), mouse anti-human CD4 AF700 (BioLegend Cat 300526) ...
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bioRxiv - Bioengineering 2020Quote: ... The non-macrophages cell fraction was then incubated in mouse CD4+ T cell isolation biotin-antiboy cocktail (BioLegend) and sorted ...
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bioRxiv - Immunology 2022Quote: ... and two hashtag antibodies were built by combining the antibodies MHC I-Biotin (Biolegend, clone 28-8-6) and CD45-Biotin (Biolegend ...
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bioRxiv - Immunology 2023Quote: The fluorochrome or biotin-conjugated primary antibodies used for flow cytometry and immunofluorescence microscopy were purchased from Biolegend, eBioscience ...
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bioRxiv - Immunology 2019Quote: ... 0.2% BSA in PBS with 0.02% sodium azide), blocked with human (humans, NHPs) or mouse (mice) TruStain FcX block (Biolegend) for 10 minutes at room temperature with shaking ...
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bioRxiv - Immunology 2021Quote: ... IL-10 in human cell culture supernatants were measured using the LEGEND Max Human IL-10 ELISA kit (BioLegend). Technical duplicates were averaged and then normalized to the total number of live cells in each sample at the time of collection.
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bioRxiv - Bioengineering 2022Quote: ... 100 IU/mL recombinant human IL-2 (Proleukin, Prometheus Laboratories) and 5 ng/mL recombinant human IL-7 (BioLegend) at 37C ...
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bioRxiv - Immunology 2023Quote: ... Human Th17 differentiation assays were performed similarly except the concentrations of recombinant human IL-6 (30 ng/ml, BioLegend), TGF- β1 (2.25ng/mL) ...
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bioRxiv - Immunology 2020Quote: ... Cells were then incubated with an anti-rabbit DyLight™649-conjugated donkey secondary Ab [clone Poly4064 (BioLegend)] for 30 minutes at room temperature (1:500 dilution) ...
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bioRxiv - Biophysics 2022Quote: ... the NMMIIA rods were labeled using 909801 anti-non-muscle-myosin IIA heavy chain primary antibody (Biolegend, rabbit) and Alexa 561 secondary (goat anti-rabbit).
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bioRxiv - Developmental Biology 2021Quote: The following antibodies and dilutions were used: rabbit anti-transformation related protein 63 (P63, 1:250, 619002, Biolegend), rabbit anti-red fluorescent protein (tdT ...
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bioRxiv - Neuroscience 2022Quote: ... and 3 washes were performed prior to secondary antibody staining with anti-rabbit bv421 1:500 (BioLegend 406410) with 3 additional washes after secondary antibody ...
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bioRxiv - Immunology 2023Quote: ... cells were washed with 3ml flow buffer (PBS with 2% FBS) at 300xg and stained with goat anti-mouse IgG-APC (BioLegend, clone Poly4053, catalog # 405308) and rat anti-mouse IgM-PE (ThermoFisher ...
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bioRxiv - Developmental Biology 2020Quote: ... or 1:100 IgG-APC isotype control (BioLegend, 400511) on ice for 30 min ...
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bioRxiv - Cancer Biology 2022Quote: ... bi-weekly or IgG (Biolegend, RTK2758; 200 µg, i.p.) bi-weekly was used to treat the mice ...
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bioRxiv - Cancer Biology 2022Quote: ... or IgG control (Biolegend, RTK2758; twice weekly i.p. 200μg) for 7 days after the initial 7 days of treatment with CXCR2smi and anti-PD1 ...
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bioRxiv - Immunology 2022Quote: ... stained with IgG-APC-Fire750 (M1310G05, BioLegend, 1:100), incubated for 30 min on ice ...
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bioRxiv - Cell Biology 2022Quote: ... armenian hamster IgG isotype control (1:400) (all BioLegend), PE-conjugate anti-CD11b (1:400) ...
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bioRxiv - Immunology 2024Quote: ... or control IgG antibody (10 ug/ml, Biolegend, #400902) for 30 mins before adding dFB-CM ...
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bioRxiv - Cancer Biology 2023Quote: ... or Armenian Hamster IgG isotype control (clone HTK888, BioLegend) was added per well ...
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bioRxiv - Genomics 2022Quote: ... and staining was performed in the presence of Fc block (eBioscience/ThermoFisher, True-Stain Monocyte Blocker (Biolegend), and Brilliant Stain Buffer (BD Bioscience) ...
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bioRxiv - Cancer Biology 2022Quote: ... Approximately 1×106 cells were aliquoted in 5 ml tubes and incubated with Fc Blocking antibody (Biolegend). Antibodies used for immune profiling are detailed in Supplementary Table 1 ...
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bioRxiv - Immunology 2021Quote: ... Following a wash, cells were incubated (RT, 10 min) with Rhesus Fc Receptor Binding Polyclonal Antibody (Biolegend). Desired staining antibodies were added directly to this mixture at indicated dilutions (Supplemental Table 27 ...
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bioRxiv - Neuroscience 2022Quote: ... in a protein low-binding microcentrifuge tube separately and incubated with 5 μL Fc blocking reagent (BioLegend) for 10 min at 4°C ...
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bioRxiv - Immunology 2022Quote: ... or plate-bound α-CD3ε (2 μg/mL) and plate-bound CD155-Fc (1 μg/mL; BioLegend) for four days ...
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bioRxiv - Developmental Biology 2022Quote: ... centrifuged and 500xg for 5 min and blocked with Fc block CD16/32 (Biolegend, #101302, 1:100), for 5-minutes on ice and stained with PECAM1-BV605 (Biolegend ...
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bioRxiv - Immunology 2019Quote: ... The following proteins and concentrations were used: ICAM-1-Fc (Cat. 553006, BioLegend, at 10 μg/mL), CD3 (clone 145-2C11 ...
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bioRxiv - Cancer Biology 2020Quote: ... 100,000–500,000 single cells of breast tumours were suspended in 100μl staining buffer (2% BSA, 0.01% Tween in PBS) before adding 10µl Fc-blocking reagent (FcX, BioLegend). and incubating during 10 min on ice ...
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bioRxiv - Immunology 2022Quote: ... and then incubated with Fc block TruStain FcX (CD16/32) to block nonspecific FcR binding (#422302, Biolegend). To detect MHC-I and MHC-II ...
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bioRxiv - Microbiology 2022Quote: ... 1×106 cells were plated and then incubated with Fc block (Biolegend; cat#101320; 1:100 dilution) and surface stained for 20 min at 4°C in the dark ...
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bioRxiv - Immunology 2024Quote: ... Cells that were used in CITE-seq experiments were blocked with TruStain Fc block (BioLegend; Cat. 422301) for 30 minutes ...
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bioRxiv - Immunology 2023Quote: ... Cells were resuspended in washing buffer (PBS 0.04% BSA) and incubated with mouse Fc blockers (FcX, Biolegend). Each sample was then incubated with either TotalSeq anti-mouse Hashtag Antibody 1 (Biolegend #155861 ...
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bioRxiv - Molecular Biology 2023Quote: ... 0.01% NaN3 in 1X PBS) and blocked with Fc receptor antibody specific for FcyR III/II (Biolegend TruStain FcX PLUS anti-mouse CD16/32 ...
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bioRxiv - Immunology 2023Quote: ... Cells were resuspended in semi-facs buffer in the presence of Fc receptor blocking antibody (BioLegend, 101302) and stained with antibodies at a 1:200 dilution with the myeloid or lymphoid antibody cocktails ...
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bioRxiv - Bioengineering 2024Quote: ... cells were stained with live dead cell staining dye NIR and then incubated with Fc block (BioLegend). Cells were further stained with the following fluorochrome-conjugated antibodies ...
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bioRxiv - Immunology 2021Quote: The following antibodies were used for the analysis of human synovial cells with cell sorting: APC anti-CD3 (Clone OKT3, BioLegend Cat# 317317, RRID:AB_1937213), PE anti-CD45RO (Clone UCHL1 ...
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bioRxiv - Bioengineering 2022Quote: ... Cells were stained for 20 minutes on ice with 1µg of BioLegend TotalSeq-A anti-human hashtag oligo antibody (BioLegend TotalSeq-A0251 to A0258). Cells were washed 3 times then pooled at equal ratios for sequencing ...
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bioRxiv - Immunology 2022Quote: ... 2×107 cells resuspended in 0.5 ml PBS were incubated with PE-conjugated mouse anti-human CD15 antibody (BioLegend, San Diego, CA, USA) diluted 1:40 ...
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bioRxiv - Cell Biology 2021Quote: ... IHC staining of mast cell tryptase was carried using mouse anti-human Mast Cell Tryptase Antibody (catalog # 369402, RRID:AB_2566541, BioLegend®, San Diego, CA, USA) [performed by the Northwestern University Mouse Histology and Phenotyping Core] ...
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bioRxiv - Cancer Biology 2021Quote: ... freshly harvested PDX or EHS tumors were processed to single cells47 and stained with PE-conjugated anti-human CD298 PE (BioLegend; 1:50 dilution) and FITC-conjugated anti-mouse CD45 FITC (BioLegend ...
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bioRxiv - Pathology 2019Quote: ... The ears were fixed in 4% paraformaldehyde for 20 minutes at 4°C and incubated overnight in 0.05% Triton X-100 in PBS containing anti-human CD31 (Biolegend, San Diego, CA, USA) antibody conjugated to either PE or AF647 in a 1:200 dilution ...
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bioRxiv - Cell Biology 2021Quote: ... Cells were washed with 2 ml of FACS buffer followed by 300 μl of FACS buffer and resuspended in a total of 100 μl of Brilliant Violet 650 mouse anti-human CD45 (Biolegend catalog no. 304043), Alexa Fluor 700 mouse anti-human CD4 (Biolegend ...
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bioRxiv - Neuroscience 2020Quote: ... anti-neurofilament to a phosphorylated heavy chain neurofilament (NF-H; mouse anti-human monoclonal antibody SMI-34, dilution 1:100, epitope retrieval time 10 min, Biolegend 835503; Biolegend, San Diego, CA). Hemosiderin deposition was evaluated on a section with only counterstain ...
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bioRxiv - Neuroscience 2022Quote: ... human Aβ was immunodepleted from TgCM with the mouse antibody 6E10 (Purified anti-β-Amyloid, 1-16 antibody BioLegend Cat# 803004, RRID: AB_2715854) and Protein G Sepharose beads (Sigma-Aldrich) ...
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bioRxiv - Bioengineering 2024Quote: ... Sections were then stained overnight at 4 °C in a humidified chamber with mouse monoclonal anti-human CD68 (hCD68, 1:400, BioLegend, clone Y1/82A) and rabbit polyclonal anti-mouse IL-10 (mIL-10 ...
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bioRxiv - Neuroscience 2023Quote: ... blood cells were lysed using VersaLyse buffer and blood and CSF cells were stained using the following anti-human antibodies (Biolegend; clone names indicated): CD3 (UCHT1) ...
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bioRxiv - Biophysics 2023Quote: ... Flow cytometry is further used to determine the expression profile of the integrins CD29 (PE anti-human CD29 antibody; Biolegend, cat. no. 303003) and CD18 (PE anti-human CD18 antibody ...
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bioRxiv - Cell Biology 2023Quote: Apical supernatant from cells at each harvest time point was assessed using LEGENDPlex Human Anti-Virus Response Panel (BioLegend, San Diego, CA, USA), as per the manufacturer’s instructions and using a FACSCanto II flow cytometer (BD Biosciences ...
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bioRxiv - Microbiology 2023Quote: ... Samples were stained immediately after fixation with Alexa Fluor 647-labeled mouse-anti-human CD56 (10 μg/mL, Biolegend, San Diego, CA, USA) for 1 h and washed with PBS (Gibco ...
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bioRxiv - Microbiology 2023Quote: ... was added to measure and gate on the % live HL-60 cells and anti-mouse/human CD11b PE (Biolegend, Catalog #101207, 1:50) was added to measure and gate on differentiated HL-60 cells ...