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Citations for BioLegend :
3201 - 3250 of 3651 citations for Annexin V Solution APC since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2021Quote: ... and treated with 1x RBC lysis solution (BioLegend). Cells were again filter using 40-μm cell strainer (BD Bioscience ...
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bioRxiv - Immunology 2023Quote: ... development was stopped with TMB Stop solution (BioLegend), and plates were read at 450 nm.
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bioRxiv - Immunology 2023Quote: ... Human TruStain FcX (Fc receptor blocking solution, BioLegend) was used to avoid nonspecific binding ...
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bioRxiv - Immunology 2024Quote: ... and 1X Brefeldin A solution (from 1000X, BioLegend) was added and incubation continued for another 5 h prior to the staining of NKG2A or NKG2C expressing NK cells ...
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bioRxiv - Bioengineering 2023Quote: ... followed by blocking with Trustain FcX solution (Biolegend) and staining with anti-human CD33 antibody conjugated to Brilliant Violet 605 (BV605 ...
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bioRxiv - Cell Biology 2022Quote: ... One hundred microliters TMB substrate solution (BioLegend, 421101) was developed for 10 minutes in dark or until reach the desired color ...
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bioRxiv - Immunology 2024Quote: ... and incubated with Fc Receptor Blocking Solution (Biolegend) at 20mg/mL for 10 minutes on ice ...
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bioRxiv - Immunology 2022Quote: ... Blocking and antibody solutions were further supplemented with 1:150 Human TruStain FcX™ Fc Receptor Blocking Solution (BioLegend, London, UK), to reduce non-specific binding ...
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bioRxiv - Immunology 2021Quote: ... and cells were stained with 1.5 μg/ml Brilliant Violet 605™ anti-CD16 (clone: 3G8) and 1 μg/ml APC anti-CD18 (clone: 1B4/CD18) (Biolegend). Cell data was collected using an Attune flow cytometer (Thermo Fisher Scientific ...
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bioRxiv - Immunology 2022Quote: ... we washed the cells twice with phosphate-buffered saline and incubated them with anti-TCRα/β allophycocyanin (APC) antibodies (clone R73, BioLegend) for 20 minutes ...
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bioRxiv - Cell Biology 2022Quote: ... Human cell subpopulations were detected in the bone marrow of transplanted mice using the following antibodies: anti-human CD45-APC (Biolegend, 368512), anti-human CD3-Pacific Blue (Biolegend ...
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bioRxiv - Cancer Biology 2020Quote: ... After 48 h incubation, cells were collected and stained with anti-CD3-FITC (BioLengend, 300406) and anti-CD69-APC (BioLegend, 310910) for 30 min at 4 °C ...
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bioRxiv - Immunology 2021Quote: ... 0.1% BSA) before secondary detection with 1:100 dilutions of APC- conjugated mouse anti-hemagglutinin tag (HA).11 antibody (BioLegend, 901524), PE-conjugated goat anti-human IgG polyclonal antibodies (Southern Biotech ...
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bioRxiv - Biochemistry 2020Quote: ... cells were incubated for 30 minutes at 4°C in PBS with a fluorochrome-conjugated antibody (mouse monoclonal APC-conjugated anti-CD69 (BioLegend 10909); mouse monoclonal PE-conjugated anti-CD25 (Pharmingen 30795X ...
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bioRxiv - Cancer Biology 2021Quote: ... Cells were then incubated with APC-conjugated anti-human HLA antibody (clone W6-32; cat. no. 311410; BioLegend, San Diego, CA). Cells were subsequently analyzed by flow cytometry for GFP cells/DsRed cells (sensitive/resistant ...
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BRD2 inhibition blocks SARS-CoV-2 infection by reducing transcription of the host cell receptor ACE2bioRxiv - Cell Biology 2021Quote: ... CD81 expression was measured 7 days post-transduction by dislodging cells with TrypLE and live cells were stained with APC-conjugated anti-human CD81 antibody (Biolegend 349509). CD81 expression was assessed on a BD LSRII with >90% of Calu3-CRISPRi cells with CD81 knocked down compared to a non-targeting sgRNA control.
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bioRxiv - Cell Biology 2021Quote: ... CD59 (PE, 143103), PD-1 (BV605, 135220), CD44 (BV711, 103057), CD69 (PE-Cy7, 104512), IFN-γ (APC, 505810) (all from Biolegend). The Foxp3 Fix/Perm Kit was used for intracellular staining (421403 ...
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bioRxiv - Cell Biology 2021Quote: ... Mac-1-PE-Cy7 and FcγR-PE (eBioscience, 12-0161-83) for myeloid differentiation, or Mac-1-APC (eBioscience, 17-0112-82) and CD19-PB (BioLegend, 115523) for lymphoid differentiation on OP9 cells ...
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bioRxiv - Immunology 2022Quote: ... 40592-V49H7-B) conjugated with PE-streptavidin or APC-streptavidin (TotalSeq-C0971 Streptavidin, BioLegend, 405271 and TotalSeq-C0972 Streptavidin, BioLegend, 405273), 0.0032 μg SARS-CoV-2 WT biotinylated RBD protein (His & AVI Tag ...
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bioRxiv - Immunology 2022Quote: ... 0.0032 μg SARS-CoV-2 biotinylated RBD protein (His & AVI Tag) (SinoBiological, 40592-V27H-B) conjugated with APC-streptavidin (BioLegend, 405207), and 5 μL 7-AAD (Invitrogen ...
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bioRxiv - Developmental Biology 2021Quote: ... The following antibodies were added at the concentration of 1:50 and incubated on ice for 45 minutes: APC/Cy7 Cd45 (Biolegend #103116), APC Pecam1 (Biolegend #102410) ...
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bioRxiv - Cell Biology 2021Quote: ... the cell pellets washed twice with 10% FCS in PBS (100μl/well) and then resuspended in 10% FCS in PBS (100μl/well) containing APC/Cyanine7 Goat anti-mouse IgG (Biolegend; 1:250) and incubated on ice for 30mins ...
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bioRxiv - Cell Biology 2021Quote: ... The following antibodies were used for cell sorting of lin-CD34+GFP+BFP+ HSPCs after CRISPR editing: CD34 APC (Biolegend 343510), CD11b APC-Cy7 (Biolegend 301342) ...
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bioRxiv - Immunology 2022Quote: ... the same panel was used with the replacement of CD4 BV750 antibody with CD8 APC (clone 53-6.7) (Biolegend Cat. 100712) and probing for IFNγ only ...
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bioRxiv - Immunology 2022Quote: ... mouse anti-human CD47 antibody conjugated to APC (323123) and anti-HA Alexa Fluor® 488 anti-HA.11 Epitope Tag (901509) were purchased from Biolegend. The antibody to ORF3a was a gift from Prof ...
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bioRxiv - Cell Biology 2022Quote: ... The samples were then treated with 25 μl of APC-antiHA antibody with 1:75 dilution (~0.07 μg/well, BioLegend catalog # 901523) for 1 hour on ice in the dark ...
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bioRxiv - Biochemistry 2020Quote: ... Cells were blocked with PBS containing 3% BSA and stained with either APC-conjugate anti-human CD99 antibody clone 3B2/TA8 (1:20 dilution, BioLegend 341307), PE anti-human CD166 clone 3A6 (1:20 dilution ...
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Immunoresolvents Support Skeletal Myofiber Regeneration via Actions on Myeloid and Muscle Stem CellsbioRxiv - Immunology 2020Quote: ... Cells were resupsended and incubated for 30 min on ice with primary antibodies including Sca-1-APC (Biolegend [Clone D7; 108112]), CD45-AF488 (Biolegend [Clone 30-F11 ...
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bioRxiv - Immunology 2021Quote: ... Monocyte isolation was determined with the following flow cytometry antibody panel after 10 min incubation with anti-human CD32 (StemCell – 18520): APC anti-human CD14 (Biolegend – 301808), PE-Cy7 anti-human CD3 (Biolegend – 344186) ...
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bioRxiv - Immunology 2021Quote: ... The following flow cytometry antibody panel was used to determine monocyte differentiation and M1 polarization after 10 min incubation with anti-human CD32: APC anti-human CD14 (Biolegend – 344186) BV421 anti-human CD80 (Biolegend – 305222) ...
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bioRxiv - Immunology 2021Quote: ... The purity of the sorted Treg subsets was assessed by staining the cells with FoxP3-APC mAb using the FoxP3 Fix/Perm Buffer Set for nuclear staining (Biolegend, USA). Over 95% of purity was detected in all cases analyzed ...
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bioRxiv - Immunology 2021Quote: ... and surface TCR expression was confirmed via flow cytometry on BD Fortessa using FACSDiva software using antibodies against mouse TCRβ constant region (APC-Fire750-conjugated, Biolegend 109246), clone H57-597 ...
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bioRxiv - Cell Biology 2021Quote: Analysis of CAR expression on transduced NK92 cells was done by staining with APC-conjugated anti-human CD314 (NKG2D) mAbs and anti-Flag Tag mAbs (Biolegend, USA). NKG2DLs expression in each cancer cell line was stained with recombinant human NKG2D Fc chimera (R&D Systems ...
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bioRxiv - Molecular Biology 2023Quote: ... and APC/Fire 750 conjugated anti E-Cadherin (dilution: 1:100; clone DECMA-1. All antibodies purchase from BioLegend (CA, USA). Samples were washed in 1mL 2% FBS and PBS and centrifuged at 2000rpm for 5mins ...
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bioRxiv - Cancer Biology 2023Quote: ... T cells transfected with pMAX-GFP and either pcDNA3.1-Rai or empty vector were added in a 10:1 ratio and incubated for 4 h in presence of APC anti-LAMP1 antibody (clone H4A3, Biolegend #328620). Monensin (2mM ...
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bioRxiv - Cancer Biology 2023Quote: ... centrifuged and 500,000 cells were stained in 200 µl 1x PBS plus 0.5 µg of indicated antibody for 30 min in the dark: APC anti-mouse CD274 (B7-H1, PD-L1, Biolegend #124311) or appropriate IgG isotype ...
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bioRxiv - Immunology 2023Quote: ... Single-cell suspensions were generated by passing through 70 μm filters and sequentially stained with APC anti-mouse CD45 (Biolegend #103111) and propidium iodide (Sigma ...
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bioRxiv - Bioengineering 2023Quote: ... Samples were subsequently stained with Zombie Violet (1:400) viability dye and APC-Cy7 conjugated human anti-CD45 (BioLegend, clone: 2D1) for 45 minutes at 4°C protected from light ...
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bioRxiv - Neuroscience 2023Quote: ... leptomeninges and brain cells were pelleted at 300xg for 10 minutes and incubated with anti-CD11b APC/Cy7 (BioLegend cat. #101226) and anti-CD45 BV510 (BD Biosciences cat ...
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bioRxiv - Immunology 2023Quote: ... intracellular FACS analysis use only) allophycocyanin (APC)/Cyanine7 or Brilliant Violet 421™ anti-human CD45 antibody (1:200, Biolegend, HI30), APC anti-human CD31 antibody (1:200 ...
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bioRxiv - Cell Biology 2022Quote: ... 45-5981-82), hamster anti-mouse CD48 APC (eBioscience, 17-0481-82) and rat anti-mouse CD150 PE-Cy7 (Biolegend, 115914). To examine macrophages ...
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bioRxiv - Cancer Biology 2022Quote: ... spun at 700 g for 3 minutes at 4°C and washed in MACS Buffer 3× before being incubated with the following antibodies: APC-conjugated rat anti-CD3 (5 μL/10×106 cells; Biolegend, 100408), PE-conjugated rat anti-CD8b (2.5 μL/10×106 cells ...
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bioRxiv - Neuroscience 2024Quote: ... PE/Cyanine7 anti-mouse/human CD45R/B220 Antibody (103222) and APC anti-mouse CD3 Antibody (100236) antibodies (Biolegend, San Diego, CA) were utilized for the lymphoid populations ...
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bioRxiv - Immunology 2024Quote: ... the enriched epithelial cell suspension was stained for flow cytometry cell sorting with the following antibodies and dyes: anti-EpCAM-APC-Cy7 clone G8.8 (BioLegend, cat. 118218), anti-CD45-APC clone 30-F11 (BD Biosciences ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... Following incubation on ice for 30 min., cells were pelleted, washed, and resuspended in PBS/BSA containing streptavidin-APC (1:2000 dilution in PBS/BSA, (BioLegend #405207)) and incubated for 30 min on ice prior to washing ...
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bioRxiv - Cancer Biology 2023Quote: IGSF3-Fc or normal human IgG (250 ng) and APC-conjugated anti-human IgG-Fc antibody (BioLegend, San Diego, CA, USA) was added to 1 × 105 of MS1 cells suspended in 100 µL of FACS buffer (0.5% BSA/HBSS ...
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bioRxiv - Bioengineering 2024Quote: ... separate tubes were designated for FITC anti-human CD80 Antibody and APC/Cyanine7 anti-human CD206 (MMR) Antibody (BioLegend Europe B.V.) staining with an incubation time of 30 min ...
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bioRxiv - Immunology 2022Quote: ... Cells were fixed with 4% paraformaldehyde (PFA) and stained intracellularly with IFN-γ-PE-Cy7 and Granzyme B-APC diluted in Perm-Wash buffer (Biolegend). Sample acquisition was performed on a Cytoflex-S (Beckman Coulter ...
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bioRxiv - Immunology 2022Quote: ... Cells were fixed with 4% PFA and stained intracellularly with IFN-γ-PE-Cy7 and Granzyme B-APC diluted in Perm-Wash buffer (Biolegend). Sample acquisition was performed on a Cytoflex-S (Beckman Coulter ...
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bioRxiv - Cancer Biology 2022Quote: ... Cells staining positive for anti-CD11b-Pacific blue (Miltenyi, 130-110-802) and anti-F4/80-APC Cy7 (Biolegend. cat.123117) were sorted as macrophages ...