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Citations for BioLegend :
3151 - 3200 of 3651 citations for Annexin V Solution APC since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2024Quote: ... anti-SHIP-1 (P1C1-A5, PE), human IgE (MHE-18, Alexa Fluor 647), HLA-DR (L243, APC-Cy7) (all from Biolegend); and phospho-p65 NF-κB (K10-895.12.50 ...
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bioRxiv - Genomics 2024Quote: ... Antibodies were added to stain surface markers for 30 min at 4°C (CD33 BV711, BD; CD11b APC-Cy7, BD; Zombie Violet, BioLegend). After 30min ...
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bioRxiv - Cell Biology 2024Quote: ... collected in PBS + 1% FBS and stained for ICAM-1 expression on the endothelial cell surface using APC anti-human CD54 antibody (353111, Biolegend). Flow cytometry analysis was performed and confirmed that ICAM-1 expression was upregulated following overnight stimulation with TNF in each of the co-culture experiments.
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bioRxiv - Immunology 2024Quote: ... which were obtained from indicated sources: APC anti-mouse I-A/I-E (MHC II) (clone M5/114.15.2, BioLegend, 1:500), FITC anti-mouse I-A/I-E (MHC II ...
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bioRxiv - Immunology 2024Quote: ... and a secondary antibody goat anti-mouse antibody conjugated with APC or rat anti-mouse antibody conjugated with Alexa Fluro 700 (BioLegend). These stained cells were fixed with fixation buffer (BioLegend ...
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bioRxiv - Immunology 2024Quote: ... Mouse anti-human HLA-E (clone 4D12) primary antibody (MBL Lifescience) and then secondary antibody goat anti-mouse conjugated with APC (BioLegend) were applied with staining buffer (DPBS ...
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bioRxiv - Microbiology 2024Quote: ... parasites were washed twice with FACS buffer and stained with (1:100) goat anti-mouse IgG-APC (BioLegend, cat# Poly4053). After incubation ...
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bioRxiv - Immunology 2024Quote: ... were isolated ex vivo from mouse peritoneal cavity by intraperitoneal lavage followed by magnetic bead-based enrichment for c-Kit+ cells using anti-CD117 (c-Kit)-APC antibody (Biolegend) and APC microbeads (Miltenyi Biotec) ...
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bioRxiv - Pathology 2023Quote: ... Ldlr−/− mice fed a chow diet or HC diet for 4 days were anesthetized using Isoflurane and injected intravenously with 5 μg anti-mouse TIM4-APC antibody (RMT4-54, BioLegend). They were then euthanized 5 minutes after injection by cervical dislocation and perfused through the portal vein with PBS and then with 4% ice-cold PFA ...
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bioRxiv - Immunology 2023Quote: ... Single cell lymphocyte suspensions were isolated as described above and stained with anti-CD45-APC (Biolegend 103112, clone 30-F11), anti-CD3e-PE (Biolegend 100308 ...
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bioRxiv - Biochemistry 2023Quote: ... Cell pellets were collected and resuspend by 500 μL (for 80 mL cord blood) Dilution Buffer and then incubated by 10 μL CD34-APC antibody (343510, BioLegend) on ice for 30 min ...
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bioRxiv - Cell Biology 2023Quote: ... PP and PC populations were identified using anti-E-cadherin-PE and anti-CD73-APC antibody staining (1:150; BioLegend). PP- and PC-specific gates were set after spectral compensation using appropriate fluorescent minus (FMO ...
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bioRxiv - Cancer Biology 2023Quote: ... and incubated for 30 minutes on ice with the following antibody: anti-CD44 APC/Fire 750 (1:100, Biolegend, 103061), anti-CD24 PE (1:20 ...
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bioRxiv - Bioengineering 2023Quote: ... Biotinylated uricase was reacted for 20 min on ice with 4:1 molar ratio of biotin to streptavidin-conjugated PE or streptavidin-conjugated APC (Biolegend). Streptavidin-conjugated FITC (BioLegend ...
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bioRxiv - Immunology 2023Quote: VLP-producing Expi293F cells were extracellularly and intracellularly stained with Allophycocyanin (APC) anti-Flag (DYKDDDDK) tag antibody (1:500) (Biolegend) and intracellularly stained with the Fluorescein isothiocyanate-labeled KC57 (anti-HIV-1 p24 ...
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bioRxiv - Immunology 2023Quote: ... Single cell suspensions were stained as above with efluor780 viability dye prior to incubation at 37C for 10 min with CCR7-APC-Fire810 (clone G043H7, Biolegend) and FcγR block ...
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bioRxiv - Physiology 2023Quote: ... the resulting single cell suspension was incubated at 37°C for 30 minutes with Integrin β1 antibody (APC anti-mouse/rat CD29; 102216; Biolegend) at 1:100 dilution ...
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bioRxiv - Genetics 2023Quote: ... Primary antibody staining was carried out at 4°C overnight with the following antibody dilutions in blocking buffer: CD52-APC (clone HI186, BioLegend) 1:200 ...
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bioRxiv - Biochemistry 2023Quote: ... Cells were stained for sgRNA expression with an APC conjugated anti-mouse CD90.1/Thy1.1 antibody (no. 202526, BioLegend; 1:400) and Human TruStain FcX Fc receptor blocking solution (no ...
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bioRxiv - Cell Biology 2022Quote: ... Cells were incubated with antibodies directly conjugated to fluorescent dyes (anti-human BAFF-R conjugated to FITC, BioLegend, Cat. #: 316904; anti-human BCMA conjugated to APC, BioLegend, Cat ...
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bioRxiv - Immunology 2022Quote: ... prior to incubation with staining antibodies (PE/Cy7 anti-mouse CD45, BV711 anti-mouse CD3, BV421 anti-mouse CD19, FITC anti-mouse CD31, and APC anti-mouse podoplanin, all BioLegend) and one Hashtag antibody per replicate using BioLegend TotalSeq-B03 (catalog numbers 155831 ...
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bioRxiv - Microbiology 2022Quote: ... the BrdU-labeled splenocytes were surface stained for T-cell (CD3e-APC; eBioscience) marker after blocking with anti-mouse CD16/32 antibodies (BioLegend). Cells were then permeabilized and treated with DNase to expose BrdU epitopes followed by anti-BrdU-FITC and 7-AAD (7-amino-actinomycin D ...
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bioRxiv - Pathology 2022Quote: ... The pellet was further washed once with FACS buffer (0.5% BSA/0.5 mM EDTA in PBS) and stained with APC-conjugated anti-mouse CD45 (1:300, BioLegend, CAT#103112), BV510-conjugated anti-mouse CD11b (1:300 ...
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bioRxiv - Immunology 2022Quote: ... Samples were then stained for extracellular markers in the presence of Fc block for 30 minutes at 4°C protected from light using the following antibodies: CD3 (APC/Cy7; 1:300; clone 17A2; BioLegend), CD4 (AF488 ...
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bioRxiv - Immunology 2023Quote: ... Cells were analyzed and sorted for transfection efficiency via flow cytometry analysis using anti-human CD3 APC/Cy7-conjugated antibody and anti-human CD34 PE-conjugated antibody for TCR-expressing cells (BioLegend, 300426 at 10 μg/mL and BioLegend ...
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bioRxiv - Bioengineering 2023Quote: ... the cells were stained for 30 min at 4°C with surface antibodies: anti-mouse CD3 (Biolegend, APC Fire 810), CD4 (Biolegend ...
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bioRxiv - Bioengineering 2023Quote: ... 50 µL aliquots of marrow cells were immunostained with 5 µL Alexa Fluor 488-conjugated rat IgG1 anti-mouse CD41 or APC-Cy7-conjugated rat IgG2b anti-mouse CD117 (BioLegend) and incubated at 4°C for 15 minutes ...
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bioRxiv - Cell Biology 2023Quote: ... Satellite cells were FACS-purified using either Pax7-nGFP reporter or the following combinations of cell surface markers: APC anti-mouse Ly-6A/E (Sca-1) Antibody (108111, BioLegend), mouse Integrin alpha 7 Alexa Fluor 750-conjugated Antibody (FAB3518S ...
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bioRxiv - Immunology 2024Quote: ... cells from each well were washed thrice with PBS and stained with APC/Cyanine7 anti-human CD14 Antibody (BioLegend, #123317) to assess THP-1 cell phagocytosis of HER2-positive tumor cells ...
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bioRxiv - Developmental Biology 2024Quote: ... For surface antigen staining cells were incubated for 20 min with APC-labelled anti-human CD56 (Biolegend, clone TS2/16) and PE-labelled anti-human CD82 (Biolegend ...
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bioRxiv - Immunology 2024Quote: ... or intracellular staining for APC-conjugated anti-human IL-17A (17-7179-42, eBioscience, USA) and Foxp3 (320014, BioLegend, USA) was performed according to the manufacturer’s instructions.
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bioRxiv - Immunology 2024Quote: ... low endotoxin (Super affinity) (Acro) followed by secondary staining with APC conjugated anti-human IgG Fc Antibody (Biolegend, cat# 410712) and GFP/RFP expressions ...
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bioRxiv - Immunology 2024Quote: ... IL-2 (anti-human IL-2, PE-Cy7, clone MQ1-17H12), and CD154 (anti-human CD154, APC, clone 24-31) all from Biolegend in Perm wash buffer (PBS + 0.02% NaN3 + 0.5% bovine serum albumin (BSA ...
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bioRxiv - Neuroscience 2024Quote: ... The cell pellets were resuspended in 100 µL of staining mix (10% FBS in FACS buffer) and 2 µL of allophycocyanin (APC) anti-HA.11 [clone 16B12] (BioLegend). This amount of antibody was empirically tested to yield the highest stain index ...
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bioRxiv - Cell Biology 2024Quote: ... BrdU was added to cell cultures 30 minutes prior to collection and fixation using the BD cytofix/cytoperm kit and staining with an anti-BrdU-APC (Biolegend) antibody ...
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bioRxiv - Immunology 2024Quote: ... ECS was performed during 15 min at room temperature with mix of anti-CD27 (O323)-FITC (or anti-IgG1 (MOPC-21)-FITC isotype control) and CD14 (QA18A22)-APC/Cy7 antibodies (Biolegend). Cells were fixed with fixation buffer (Biolegend ...
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bioRxiv - Immunology 2024Quote: ... peptide-pulsed K562/HLA-E cells were stained with APC-labeled anti-human HLA-E mAb (clone 3D12, BioLegend, USA). Then the CD94/NKG2A+HLA-E+ and CD94/NKG2C+HLA-E+ K562/HLA-E cells were gated as targeted cells.
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bioRxiv - Immunology 2024Quote: ... The purity of BMNs populations was greater than 93% as determined by flow cytometry after staining with anti-Ly6G-APC (B357443,BioLegend), and the viability was greater than 99% as determined by trypan blue staining ...
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bioRxiv - Immunology 2024Quote: ... for Fc receptors blocking and then simultaneously incubated with HpARI proteins tetramers and stained for CD45 (APC-Cy7, clone 30-F11; Biolegend). Cells were then acquired on a LSR Fortressa (BD ...
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bioRxiv - Immunology 2024Quote: ... Sections were saturated with BSA 2% PBS and then incubated overnight with an APC-conjugated anti-CD45 antibody (clone 30F11, Biolegend) at 4°C ...
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bioRxiv - Microbiology 2024Quote: ... Cells present in the suspension were stained on ice for 30 minutes with APC-conjugated anti-Ly-6G (clone 1A8) or PE-conjugated anti-CD63 (Biolegend) and then washed two times in FACS buffer (Biolegend) ...
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bioRxiv - Cancer Biology 2024Quote: ... quantifying the ratio of human CD45 (labelled with PE) to mouse CD45 (labelled with APC-Cy7) all of which were supplied by Biolegend.
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bioRxiv - Cancer Biology 2024Quote: ... 0.5 – 1 x 105 cells were washed with CSM and incubated with 100 µl antibody mix (1 µl APC anti-human CD19 (clone: HIB19, BioLegend); or 1 µl APC anti-human CD22 (clone ...
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bioRxiv - Cancer Biology 2024Quote: ... Samples were then incubated on ice in the dark with APC anti-mouse Ly-6A/E (Sca-1) Antibody (clone D7, 108111, BioLegend) antibody for 45 min ...
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bioRxiv - Cell Biology 2024Quote: ... The PP and PC populations were identified using anti-E-cadherin-PE and anti-CD73-APC antibody staining (1:150; BioLegend). The PP- and PC-specific gates were set after spectral compensation using appropriate fluorescent minus (FMO ...
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bioRxiv - Immunology 2024Quote: ... The cells were then stained for 30 mins at 4°C in the dark with anti-human antibodies specific for anti-CD14 [APC/Cyanine-7] (301819, BioLegend), anti-CD16 [Fluorescein isothiocyanate (FITC)] (360715 ...
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bioRxiv - Cell Biology 2020Quote: ... After adding 7AAD viability staining solution (420404, Biolegend), cells were measured on BD FACS Canto II flow cytometer and analysed using FlowJo (Version 10 ...
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bioRxiv - Cell Biology 2021Quote: ... Afterwards 100 μl of TMB substrate solution (BioLegend) were added and the plate was incubated about 20 min at room temperature in the dark ...
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bioRxiv - Immunology 2020Quote: ... A solution of anti-mouse CD3e (100314, Biolegend) was prepared at 5 µg/mL in sterile PBS and 50 μl of antibody solution was added into 96 well plate followed by incubation at 37°C for 2 hours ...
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bioRxiv - Immunology 2020Quote: ... 7-AAD viability staining solution was from Biolegend. MART-1 peptide (MART-126-35A27L ...