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Citations for BioLegend :
3101 - 3150 of 3651 citations for Annexin V Solution APC since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2020Quote: ... The purity of isolated MDSCs was checked by flow cytometry using Gr1 FITC and CD11b APC antibodies (purchased from Biolegend). MDSCs were grown in exosomes depleted media consisting of RPMI ...
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bioRxiv - Bioengineering 2021Quote: ... Naïve T cell marker staining was performed with APC/Cy7 mouse anti-human CD45RA (#304128) and BV510 mouse anti-human CD45RO (#304232) antibodies from Biolegend.
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bioRxiv - Systems Biology 2020Quote: ... cells were stained on ice for 30 min with the following fluorochrome-conjugated antibodies in a 12-color staining combination: APC anti-IL17RA (clone W15177A, BIolegend) at 1:50 ...
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bioRxiv - Cell Biology 2021Quote: ... Mononuclear cells were incubated with APC (allophycocyanin) or PE (phycoerythrin)-conjugated anti-mouse Ly-6A/E (Sca-1) antibody (#122508, BioLegend), APC or PE-conjugated anti-mouse CD45 antibody (#103106 ...
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bioRxiv - Cell Biology 2020Quote: ... Measurements were compared to the isotopic control (APC-conjugated anti-mouse IgG1, Biolegend clone MOPC-21 #BLE400122, 1/100, Ozyme) to determine background and positivity thresholds ...
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bioRxiv - Bioengineering 2022Quote: ... and Phycoerythrin-conjugated Goat anti-Mouse IgG2a Cross-Adsorbed Secondary Antibody (Fisher) for ACE2-Bite and APC anti-human IgG Fc Antibody clone HP6017 (Biolegend) for ACE2-Fc were added to the wells and incubated for another hour at room temperature ...
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bioRxiv - Immunology 2022Quote: ... Cell samples at each timepoint were prepared as described and co-stained with PE-anti-CD19 and allophycocyanin-conjugated mouse anti-human CD23 (APC-anti-CD23, BioLegend, clone EBVCS-5 ...
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bioRxiv - Immunology 2022Quote: ... The following antibodies were used to identify MKs: 1:100 anti-mouse CD41-FITC+ and anti-mouse CD42d-APC+ (BioLegend); and MKPs ...
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bioRxiv - Microbiology 2022Quote: ... and then stained for 30 minutes at 4℃ with the following antibody panel each diluted in PBS: APC/Cyanine7 anti-mouse CD45 antibody (Biolegend) or FITC anti-mouse CD45.2 antibody (Biolegend) ...
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bioRxiv - Immunology 2022Quote: ... 0.0032 μg biotinylated SARS-CoV-2 BA.1 protein (His & AVI Tag) (SinoBiological, 40592-V49H7-B) conjugated with PE-streptavidin or APC-streptavidin (TotalSeq-C0971 Streptavidin, BioLegend, 405271 and TotalSeq-C0972 Streptavidin ...
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bioRxiv - Bioengineering 2022Quote: ... at 0.5 μL/well for 5 hours and washed with PBS for detection of intracellular IFNγ using anti-hIFNγ APC antibody (Biolegend). The frequency of IFNγ positive cells was analyzed by flow cytometry (CytoFLEX II ...
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bioRxiv - Biochemistry 2022Quote: ... neutrophils (1×106 cells/mL) were added to each well in the presence of APC conjugated anti-CD11b antibody CBRM1/5 (BioLegend) at 1 μg/mL ...
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bioRxiv - Cancer Biology 2022Quote: The anti-CD58-APC antibody used for the CRISPR-Cas9 KO screen was titrated by staining pooled A375 WT and CD58 KO cells as above with Zombie-Violet followed by increasing concentrations of anti-CD58-APC (Biolegend) antibody ...
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bioRxiv - Immunology 2021Quote: ... fixed with 4% PFA for 15 minutes and subsequently stained for 30 minutes with anti-CD4-APC and anti-CD8-BV605 (Biolegend) in FACS buffer ...
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bioRxiv - Immunology 2020Quote: ... dilution 1:50), and CD45RO-APC-Cy7 (UCHL1, dilution 1:50), CD127-PE-Cy7 (A019D5, dilution 1:20) (all from BioLegend). FoxP3-APC (eBioscience ...
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bioRxiv - Microbiology 2021Quote: ... and incubated with anti-CoV-2 Spike RBD mAb (SARS2-02) (13) followed by APC-conjugated goat anti-mouse IgG antibody (Cat# 405308, BioLegend). For surface detection ...
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bioRxiv - Cell Biology 2021Quote: ... Populations of interest based on DAPI negativity expression and lack of lineage markers (APC-Cy7; CD31, Ter119, CD45 at 1 μL/106 cells; Biolegend) expression were directly sorted into DMEM/F12 containing 10% FBS with 1% P/S/A ...
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bioRxiv - Microbiology 2020Quote: ... Cells were then stained for 30 minutes on ice with anti-CD11b-APC/Fire 750 and anti-F4/80-PE for macrophages or anti-CD11c-Alexa Fluor 647 for dendritic cells (BioLegend). Samples were washed twice and then run on Cytoflex (Beckman Coulter) ...
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bioRxiv - Microbiology 2020Quote: ... After incubation the cells were washed twice in FACS buffer (PBS, 0.5% BSA, 2 mM EDTA) and stained with a CD19-APC antibody (BioLegend # 302212) to analyze pDCs for 20 min at 4°C to analyze B cells or with two antibodies directed against CD303(BDCA2)-APC (Miltenyi Biotec ...
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bioRxiv - Cell Biology 2021Quote: ... for 10 min at 4°C and stained with fluorophore conjugated antibodies for 20 min at 4°C (anti-Pdpn PE-Cy7, Biolegend 127411; anti-Thy1 A647, Biolegend 105318; anti-CD31 APC/Fire 750, Biolegend 102433 ...
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bioRxiv - Microbiology 2021Quote: ... PE/Cy7-conjugated mouse anti-human CD47 (clone CC2C6) monoclonal Ab (mAb) and APC-conjugated anti-CD11b mAb (clone ICRF44) as well as corresponding isotype controls from BioLegend, APC-conjugated mouse anti-human CD47 mAb (clone B6H12 ...
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bioRxiv - Immunology 2022Quote: ... Cells were then incubated for 20 minutes at RT with mouse PE conjugated 5-OP-RU MR1-tetramer (NIH Tetramer core) and the following antibodies: anti-CD4-FITC/APC-Fire810 (clone GK1.5, Tonbo Biosciences/Biolegend), anti-CD8α-AF700 (clone 53-6.7 ...
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bioRxiv - Genomics 2022Quote: ... was used according to manufacturer’s recommendations: intracellular labeling with APC anti-GATA3 antibody (2.5 ul antibody per million cells in 100 ul) (Biolegend #653806) and PE anti-DYKDDDDK Tag antibody (0.6 ul antibody per million cells in 100 ul ...
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bioRxiv - Immunology 2022Quote: ... LN and thymic homogenates were stained with a cocktail of antibodies against CD4-FITC and CD25-APC (for LN Tregulatory cells; Mouse Regulatory T Cell Staining Kit #1, eBioscience) or CD4-PB (Biolegend), CD8-FITC ...
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bioRxiv - Immunology 2021Quote: ... phagocytosis was stopped by washing with 4°C PBS and centrifugation at 1200 RPMI before the cells were stained with APC-labeled anti-CD11b (Clone M1/70, Biolegend) to identify human macrophages and Live/Dead stain (Fixable Viability Dye ...
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bioRxiv - Immunology 2020Quote: ... then cells were washed with 2% FCS-PBS and surface-stained with anti-human HLA-DR-Pacific Blue or -APC (clone L243; Biolegend) and anti-human CD123-FITC or -PeCy5 (clone 6H6 ...
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bioRxiv - Immunology 2021Quote: ... cells were collected by scraping and stained with APC-conjugated anti-mouse H-2Kb bound to SIINFEKL antibody (141606, BioLegend) and PerCP-Cy5.5-conjugated anti-mouse CD86 antibody (105027 ...
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bioRxiv - Cancer Biology 2020Quote: ... Cells were washed with PBS for three times and incubated with rat anti-mouse IgG2a conjugated with APC (407109, BioLegend) for 1 hour at room temperature ...
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bioRxiv - Cancer Biology 2020Quote: ... Single tumor cells were resuspended to 5 × 107 cell/mL and stained with CD298-APC (10 µL/106 cells)(BioLegend) and Sytox Green (ThermoFisher ...
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bioRxiv - Cell Biology 2020Quote: Single cell suspension was generated from adult lungs and stained with antibodies: anti-EpCAM (APC-Cy7-conjugated, Biolegend,1:50), CD49F (APC-conjugated ...
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bioRxiv - Immunology 2021Quote: ... Cells were stained with fluorescently-labeled antibodies directed against cell-specific surface marker for 30 min one ice in the dark (anti-mouse CD45-APC/Cy7, #103116, BioLegend; anti-mouse CD45-FITC ...
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bioRxiv - Immunology 2021Quote: ... IgD-BV510 (clone 11-26c.2a), CD138-BV605 (clone 281-2), CD38-PE-Cy7 (clone 90, CD27-APC (clone LG-3A10) (Biolegend), CD4-FITC (clone GK1.5 ...
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bioRxiv - Molecular Biology 2021Quote: ... CD4+ or CD8+ cells were stained with an APC-Cy7-labeled anti-CD4 and FITC-labeled anti-CD8 antibody (Biolegend), respectively ...
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bioRxiv - Immunology 2020Quote: ... Anti-CD69 (H1.2F3, PE/Cy7, B253212, 104512), Anti-CD3 (17A2, APC/Cyanine7, B283940, 100222) antibodies were purchased from BioLegend (Bxxxxxx) or BD Biosciences (xxxxxxx).
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bioRxiv - Immunology 2021Quote: ... Cells were then washed in 1x eBioscience Permeabilization Buffer twice and incubated with indicated intracellular antibodies including anti-TNF-PE (MAb1) and anti-IFN-γ-APC (4S.B3) from BioLegend at room temperature for 1 hour ...
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bioRxiv - Immunology 2021Quote: ... followed by selection of memory B cells by positive expression of CD20 and CD27 (CD20-FITC, clone 2H7; CD27-APC-Cy7, clone O323, both BioLegend) but not immunoglobulin D (IgD-PE ...
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bioRxiv - Microbiology 2021Quote: ... prior to staining cells in 100 µL perm/wash with 0.5 µL APC anti-human IL-6 antibody (Cat# 501112, Biolegend®) overnight at 4°C ...
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bioRxiv - Physiology 2023Quote: ... Samples were next stained with surface marker antibodies (CD45-AF700 1:500 103128 Biolegend, CD31-Pacific Blue 1:500 102421 Biolegend, TER119-BUV496 1:500 741079 BD, PDGFRα-APC 1:500 135907 Biolegend, ITGβ4-AF405 1:500 FAB405V Thermofisher ...
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bioRxiv - Immunology 2023Quote: ... Cells were permeabilized for 20 mins at 4°C and then stained overnight with IFN-γ-APC (clone 4S.B3, Biolegend) antibody at 4°C.
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bioRxiv - Cell Biology 2023Quote: ... Cells were incubated with the following fluorochrome-conjugated antibodies: APC/Fire™ 750 anti-human CD324 (E-Cadherin) (Biolegend 324122), PE anti-human CD326 (EpCAM ...
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bioRxiv - Microbiology 2023Quote: ... cells were washed and stained by incubation at 4 °C for 30 min with 40 µl of mAb anti-membrane marker mix containing CD4 APC/Cy7 (Biolegend), CD19 FITC ...
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bioRxiv - Immunology 2023Quote: ... Pacific Blue (or v450), and APC-Cy7 (or Alexa Fluor-e780)-conjugated antibodies (1:100, CD4, CD8) purchased from Biolegend. After cell surface marker staining ...
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bioRxiv - Immunology 2023Quote: ... Pooled cells from n = 3 - 5 mice/cohort were stained with PE- or APC-conjugated antibodies (from either BD or BioLegend) against murine F4/80 (clone ...
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bioRxiv - Immunology 2023Quote: ... anti-CD25(Percp-Cy5.5, #302626), anti-CD69(APC, #310910), anti-mouse CD25(PE, #12-0251-83), anti-mouse CD69(FITC, #104506) from Biolegend, anti-human IgG(SureLight® PE ...
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bioRxiv - Immunology 2023Quote: ... electroporated Jurkat cells were interrogated by pMHC-multimer staining with the following surface panel: anti-CD3 APC Fire 50 (SK7, Biolegend), anti-CD8 Pacific Blue™ (RPA-T8 ...
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bioRxiv - Genomics 2022Quote: ... eF450 (anti-GL7, eBioscience, 48-5902-82), APC (anti-CD86, eBioscience, 17-0862-82) and PE (anti-CXCR4, BioLegend, 146505). Also ...
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bioRxiv - Bioengineering 2024Quote: ... Biotinylated rasburicase was reacted for 20 min on ice with 4:1 molar ratio of biotin to streptavidin-conjugated PE or streptavidin-conjugated APC (Biolegend). Streptavidin-conjugated FITC (BioLegend ...
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bioRxiv - Cancer Biology 2024Quote: ... The dissociated tumor cells were stained with AF488 anti-CD8 antibody and APC anti-CD11c antibody (BioLegend, San Diego, CA) and subjected to flow cytometry analysis to access infiltrated immune cells in the tumor microenvironment ...
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bioRxiv - Immunology 2024Quote: ... Following volumes were added in a single tube containing 100ul FACS buffer per sample: 0.625uL per sample of APC/Cyanine7 anti-mouse CD19 (Biolegend 152412), 1.25uL per sample of Brilliant Violet 421™ anti-mouse CD38 (Biolegend 102732) ...
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bioRxiv - Immunology 2024Quote: ... in the presence of S2 mAbs (5 μg/mL), protein transport inhibitor cocktail (1:500, eBioscience) and anti-CD107a APC (clone H4A3, BioLegend). Antibody-dependent NK cell activation was allowed to occur for 4 hours at 37°C and 5% CO2 ...