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Citations for BioLegend :
2351 - 2400 of 2559 citations for TD 5108 CAS 866933 46 2 99% since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2022Quote: ... 100μg/ml streptomycin and 2 mM EDTA) and stained for 20 minutes at 4 degrees with the following fluorescently conjugated antibodies (BioLegend): anti-CD11c (clone N418) ...
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bioRxiv - Immunology 2022Quote: ... mice were inoculated with CEA10-mRFP FLARE conidia and lung cells were incubated with 0.05 mg/ml of pHrodo-Zymosan at 37 °C for 2 hours and stained with Survival dye (efluor780, eBioScience) and Ly6G (Pb, BioLegend) or Ly6G (Pb ...
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bioRxiv - Immunology 2022Quote: ... CD4+ T-cells were cultured alone or co-cultured with either of the B-cell populations at a ratio of 3:1 for 60 h at 37°C and 5% CO2 on anti-CD3 (2 μg/mL) (ULTRA-LEAF Biolegend) coated flat bottomed 384 CellCarrier Ultra microplates (PerkinElmer ...
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bioRxiv - Immunology 2022Quote: ... Single cell suspensions were resuspended in PBS with 2% Newborn Calf Serum and stained with TruStain FcX PLUS (S17011E, BioLegend), APC-labeled Ly6G (1A8 ...
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bioRxiv - Immunology 2022Quote: ... resuspended in 50 μl FACS-buffer containing the respective fluorochrome-coupled Abs (anti-TACI/CD267 PE, clone eBio8F10-3, eBioscience, anti CD138 PECy7, clone 281-2, Biolegend, anti CD19 BV510 ...
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bioRxiv - Cancer Biology 2023Quote: ... Cells were then diluted with 50 μL PBS + 2% FBS and FITC anti-CD45 (BioLegend, 304005; 1:1,000 final dilution) and incubated for 20 min on ice in the dark ...
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bioRxiv - Immunology 2023Quote: ... Cells were sorted and purified cells were stained on ice for 30 min in PBS containing 1 % (w/v) BSA with hashtag antibodies (TotalSeq-B0301/B0302/B0303/B0304B0305 anti-mouse Hashtag 1/2/3/4/5, respectively, Biolegend). Cells were washed ...
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bioRxiv - Evolutionary Biology 2022Quote: ... Cells were centrifuged at 500× g at 4 °C and resuspended in 1/3 strength artificial seawater containing 2 μg/mL calcein AM (Enzo) and 100 nM sytox blue (BioLegend) to monitor viability ...
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bioRxiv - Immunology 2023Quote: ... 0.5-1×106 bone marrow cells were stained with the following surface antibodies (2 panels) against: CD14 (Biolegend, M5E2, AF700), HLA-DR (Biolegend ...
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bioRxiv - Cancer Biology 2023Quote: ... 2×106 bone marrow cells were cultured at 37°C in 10ml bmDC culture medium (RPMI 1640 medium with 10% FBS, penicillin/streptomycin and 2-Mercaptoethanol) with 40ng/mL GM-CSF (BioLegend). At day 3 ...
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bioRxiv - Immunology 2023Quote: ... The cells were resuspended in 1 ml R10 and 1-2 million cells were plated in 1ml R10 with 1 μl Brefeldin A (BioLegend) for 4 hours ...
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bioRxiv - Immunology 2024Quote: ... and APC anti-ligand (B7-H6: R&D, clone 875001; CD48: Biolegend, clone; BJ40; Nectin-2: Biolegend, clone TX31; LFA-3: Biolegend, cloneTS2/9 ...
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bioRxiv - Immunology 2024Quote: ... samples were stained on ice for 45 minutes in PBS with 2% Newborn Calf Serum and True-Stain Monocyte Blocker (BioLegend) using a combination of the following antibodies ...
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bioRxiv - Immunology 2024Quote: ... samples were incubated overnight at 4°C with combinations of the following antibodies: PE-Cy7-labeled CD63 (NVG-2, BioLegend), AF532-labeled iNOS (CXNFT ...
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bioRxiv - Genomics 2024Quote: ... A Zombie Violet viability staining was performed by resuspending the cells in 1 mL PBS and adding 2 µl of Zombie Violet (1:1000 dilution, Biolegend). The cells were then kept for 10 minutes in the dark ...
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bioRxiv - Immunology 2024Quote: ... IL-2 (anti-human IL-2, PE-Cy7, clone MQ1-17H12), and CD154 (anti-human CD154, APC, clone 24-31) all from Biolegend in Perm wash buffer (PBS + 0.02% NaN3 + 0.5% bovine serum albumin (BSA ...
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bioRxiv - Neuroscience 2024Quote: ... The cell pellets were resuspended in 100 µL of staining mix (10% FBS in FACS buffer) and 2 µL of allophycocyanin (APC) anti-HA.11 [clone 16B12] (BioLegend). This amount of antibody was empirically tested to yield the highest stain index ...
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bioRxiv - Microbiology 2024Quote: ... in the presence of 10 ng/mL recombinant human IL-2 and 10 ng/mL of recombinant human IL-15 (BioLegend) for 3 days ...
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bioRxiv - Immunology 2024Quote: ... Alexa Fluor 594 conjugated anti-hCD19 (mouse IgG1, R & D Systems) and Alexa Fluor 647 conjugated anti-human CD340 (erbB2/HER-2, Biolegend) antibodies were used for CEACAM5 ...
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bioRxiv - Immunology 2024Quote: ... 4×106 cells from spleen were plated in a 96-well plate and incubated with SIINFEKL peptide and anti-CD107a for 2 hours at 37°C before Brefeldin A (5.0 mg/mL: Biolegend, 420601) and Monensin protein transport inhibitors (4 pL/6E6 cells ...
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bioRxiv - Immunology 2024Quote: ... Specific IgG and IgM antibody levels were measured in culture supernatants by ELISA using LEGEND MAXTM SARS-CoV-2 Spike S1 Human IgG ELISA kit (BioLegend) and LEGEND MAXTM SARS-CoV-2 Spike S1 Human IgM ELISA kit (BioLegend) ...
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bioRxiv - Immunology 2024Quote: ... Autologous CD8+ T cells were isolated from PBMCs using EasySep human CD8+ T cell isolation kit (TEMCELL, Cat# 17953) and stained with 2 μM CFSE (BioLegend) in PBS at r.t ...
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bioRxiv - Immunology 2024Quote: ... and fluorochrome-conjugated anti-CD86 and anti-CD32 antibodies in FACS buffer (1%BSA and 2 mM EDTA in PBS) supplemented with True Stain Monocyte Blocker solution (Biolegend) to prevent aspecific binding of fluorochrome-conjugated antibodies to myeloid cells ...
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bioRxiv - Immunology 2024Quote: Cells were collected and washed in FACS buffer (phosphate-buffered saline supplemented with 2% fetal bovine serum) and subsequently stained with the following antibodies purchased from BioLegend ...
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bioRxiv - Immunology 2024Quote: PBMCs (2×107) of HFRS patients were stained with PE-Cy7-labeled anti-human CD3 mAb (Clone 17A2, BioLegend, USA), APC-Cy7-labeled anti-human CD56 mAb (Clone 5.1H11 ...
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bioRxiv - Neuroscience 2024Quote: ... anti-IgD Pacific Blue (Clone IA6-2, 348224), anti-IgM BV570 (Clone MMH-88, 314517) and Zombie NIR fixable viability dye (423106, Biolegend). Cells were then washed in PBS (D8537 ...
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bioRxiv - Cancer Biology 2024Quote: ... Surface staining of the Macrophage panel consisted of the following primary antibodies diluted in FACS buffer (2% FBS in DPBS) with anti-mouse CD45-Brilliant Violet 650 (1:200, 103151, Biolegend), anti-mouse F4/80-Brilliant Violet 785 (1:200 ...
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bioRxiv - Immunology 2024Quote: ... Sections were saturated with BSA 2% PBS and then incubated overnight with an APC-conjugated anti-CD45 antibody (clone 30F11, Biolegend) at 4°C ...
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bioRxiv - Microbiology 2024Quote: ... LAMP-1 was stained as described previously for LAMP-2 but using a rat-IgG anti-mouse LAMP-1 and anti-rat-IgG-AlexaFluor647 (Biolegend) [56] ...
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bioRxiv - Immunology 2024Quote: Surface staining of lung single cell suspensions was performed in PBS with 2% Newborn Calf Serum on ice for 30 minutes with the following antibodies: TruStain FcX PLUS (S17011E, BioLegend), APC-labeled Ly6G (1A8 ...
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bioRxiv - Molecular Biology 2024Quote: ... The cells were washed with PBS containing 2 mM EDTA and stained with antibodies against CD59 (1:100, anti-human CD59 PE, Biolegend) and C3c (1:25 ...
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bioRxiv - Cell Biology 2024Quote: ... and the supernatant was used for ELISA analysis of IL-2 and latent TGF-beta secretion levels by the kits from Biolegend according to manufacturer’s instructions.
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bioRxiv - Immunology 2021Quote: ... Cells harvested during the conversion period from CD34+ hematopoietic precursors to CD14+ monocytes (Days 12-21 of the differentiation protocol) were stained with αCD14-PE (BioLegend, San Diego, CA, USA) and αCD34-APC (BioLegend) ...
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bioRxiv - Immunology 2021Quote: ... Then cells were washed with PBS and incubated with anti-HMGB1 conjugated with phycoerythrin (PE) (# 651403, PE anti-HMGB1; Biolegend, San Diego, CA, USA) for 40 min ...
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bioRxiv - Cell Biology 2020Quote: ... for 10 minutes at 4°C followed by staining with antibody cocktail containing Pacific-Blue-conjugated anti-mouse CD45 (clone 30-F11; Biolegend, San Diego, CA, USA), PE-conjugated anti-mouse Ly-6G (Gr-1 ...
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bioRxiv - Neuroscience 2020Quote: ... Cells were then incubated for 1 hour at 37 °C with different primary antibodies: anti-β-III-tubulin (1:4000, 801202, Biolegend, San Diego, CA), anti-neurofilament SMI312 (1:1000 ...
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bioRxiv - Neuroscience 2020Quote: The cerebellar slices were treated either 3 times 24 hours apart with combinations of recombinant TNF (recombinant rat TNF, Biolegend, San Diego, CA, USA), LTα (recombinant human LT-α ...
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bioRxiv - Bioengineering 2021Quote: The Zombie Aqua™ Fixable Viability dye and all primary antibodies used for flow cytometry analysis were obtained from BioLegend (San Diego, CA, USA) (Table 1).
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bioRxiv - Bioengineering 2021Quote: ... IL-6 was quantified using ELISA developed in the laboratory using biotin anti-rat IL-6 antibody (cat. # 517703) and purified (coating) anti-rat IL-6 antibody (cat. # 517701, Biolegend, San Diego, CA, USA). Coating antibody was diluted in 100 mM NaHCO3 ...
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bioRxiv - Immunology 2020Quote: ... cells in black wall 96-well-plate were washed with PBS for once and blocked with FcR blocking buffer (FcX block, Biolegend, San Diego, CA, USA) at room temperature for 10 minutes ...
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bioRxiv - Microbiology 2021Quote: Concentrations of cytokines and chemokines present in the serum from SARS-CoV-2 infected AGMs were quantified using a multiplex bead-based assay (1:4 dilution)-the LEGENDPlex Non-Human Primate Cytokine/Chemokines 13-plex (BioLegend, San Diego, CA USA). Analytes detected by this panel are the following ...
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bioRxiv - Microbiology 2021Quote: ... transferred to a nitro-cellulose membrane and probed with polyclonal rabbit anti CupA (1:1000) and monoclonal mouse anti-RNA polymerase (1:3000, Biolegend, San 397 Diego, CA). Western blots were then probed with IRDye-conjugated anti-mouse and anti-rabbit secondary antibodies (both at 1:15,000 ...
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bioRxiv - Bioengineering 2020Quote: PDCD1 and B2M expression on EL4 cells were assessed with fluorescent antibody staining using an APC-conjugated PD1 antibody (BioLegend, San Diego, CA: 135210) or AlexaFluor 647-conjugated H-2Kb antibody (BD Biosciences ...
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bioRxiv - Cancer Biology 2021Quote: ... Further preparation for FACS measurement was following the protocol of the BioLegend APC Annexin V Apoptosis Detection Kit and DNA-staining with DAPI Reagent (25 μg/mL) (Biolegend, San Diego, CA, USA). 20 000 cells were assayed using a flow cytometer FACSCantoII (Becton Dickinson ...
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bioRxiv - Bioengineering 2022Quote: ... differentiated cells were again lifted off their culture surfaces using TrypLE Select and subjected to fluorescence activated cell sorting (FACS) after being stained with an erbB3 antibody (Biolegend, San Diego, CA, USA) conjugated to phycoerythrin ...
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bioRxiv - Cancer Biology 2022Quote: ... was analyzed and monitored longitudinally by weekly bleeding to quantify hCD45+ cells in the peripheral blood by flow cytometry with human CD45-PE and anti-mouse CD45-APC-Cy7 antibodies (Biolegend, San Diego, CA, USA). Mice were monitored closely to detect disease onset and treatment started when hCD45+ cells were detectable in the peripheral blood ...
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bioRxiv - Immunology 2022Quote: ... and resulting cells were cultured in complete RPMI-1640 medium containing various concentrations of recombinant murine GM-CSF (rGM-CSF, BioLegend, San Diego, CA, USA), GPI-GM-CSF ...
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bioRxiv - Physiology 2020Quote: ... and MDA-MB-468/sh cells were grown and subjected to incubation with fluorescein isothiocyanate (FITC)-conjugated anti-CD24 (Cat. #311104; BioLegend, San Diego, CA, USA) and PE-conjugated anti-CD44 (Cat ...
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bioRxiv - Cancer Biology 2021Quote: ... at a dilution of 1:200 for 30 min at room temperature followed by incubation with horseradish peroxidase streptavidin at a dilution of 1:125 (405210, BioLegend, San Diego, CA, USA) for 30 min at room temperature ...
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bioRxiv - Cancer Biology 2021Quote: ... The samples were blocked with 0.5 % BSA for 10 min on ice and incubated with the primary mAbs biotin-anti-human CD274 (B7-H1, PD-L1) (clone 29E.2A3, BioLegend, San Diego, CA, USA) or biotin anti-mouse CD274 (clone 10F.9G2 ...