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Citations for BioLegend :
2251 - 2300 of 2559 citations for TD 5108 CAS 866933 46 2 99% since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2022Quote: ... stained for 20 minutes on ice with the following antibodies at the following concentrations: anti-mouse CD49b 1:500 (#103511 Biolegend, San Diego, CA), anti-human CD49b 1:750 (#359310 Biolegend) ...
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bioRxiv - Cell Biology 2022Quote: ... and the cells were resuspended in 200 μL biding buffer with 2μL 7-AAD and 2μL annexin V-FITC (Biolegend Inc, San Diego, CA, USA). The plate was incubated for 15 min in darkness and measured in the flow cytometer FC 500 (Beckman-Coulter ...
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bioRxiv - Cancer Biology 2022Quote: ... Cells were then stained for viability using Zombie NIR™ fixable viability kit for 15 min at room temperature (Cat # BioLegend, CA, USA), followed by fluorescently labeled high- or low-molecular weight HA (2.5mg/ml in PBS ...
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bioRxiv - Cell Biology 2022Quote: Cytokine levels in the plasma and cell culture medium were detected using the LEGENDplex™ Human Th Panel (BioLegend, San Diego, CA, USA) containing 13 human antibodies targeting IL-5 ...
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bioRxiv - Cancer Biology 2022Quote: ... The cells were incubated with Live/Dead fixable violet dead cell stain [Thermo Fisher Scientific (Waltham, MA)] for the live cell gating and with the following antibodies from Biolegend (San Diego, CA): CD16/32 ...
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bioRxiv - Immunology 2022Quote: ... Cells were then fixed and permeabilized for 30 minutes followed by intra-nuclear staining for FOXP3 for an additional 4hours using FOXP3 Fix/Perm Buffer set (Biolegend, San Diego, CA).
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bioRxiv - Immunology 2022Quote: Activated γδT cells were seeded in round-bottom plates with FITC conjugated anti-mouse CD107a (1D4B) antibody or anti-human CD107a (H4A3) (Biolegend, San Diego, CA). γδT cells were co-cultured with A20 or K562 at a ratio of 1:1 ...
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bioRxiv - Immunology 2023Quote: ... prior to the end of the CD45- sort one vial of TotalSeq-C Human Universal Antibody Cocktail v1.0 (Biolegend, San Diego, CA, USA) was equilibrated to room temperature for 5min and then spun at 10,000g for 30 seconds ...
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bioRxiv - Immunology 2023Quote: Biotinylated pneumococcal serotype 3 polysaccharide with a 5% biotin load was coupled with high concentration streptavidin-phycoerythrin (SA-PE) and streptavidin-allophycocyanin (SA-APC, both from BioLegend, San Diego, CA) in separate reactions ...
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bioRxiv - Cancer Biology 2023Quote: ... dendritic cell markers were purchased for characterization of immune cells using flow cytometry: PerCP/Cy5.5 anti-human CD3 and CD11c (Biolegend, San Diego, CA, USA), PE anti-human CD4 and CD56 (Biolegend) ...
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bioRxiv - Immunology 2024Quote: ... Cell pellets were resuspended in 100 µL of Fc-blocking medium (5 µg/ml Purified anti-mouse CD16/32 Antibody (Biolegend, San Diego, CA) in FACS buffer) ...
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bioRxiv - Immunology 2024Quote: ... anti-IgD (follicular B cell marker, Alexa Fluor® 647 anti-mouse IgD antibody, 11-26c.2a, catalog #405708, Biolegend, San Diego, CA), anti-B220 (B cell marker ...
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bioRxiv - Immunology 2024Quote: ... as well as Alexa fluor 647-coupled Goat anti-Mouse (AF647; Poly4053) and APC-coupled Streptavidin were purchased from Biolegend (San-Diego, CA). Antibodies specific for SNAT2 (C-6) ...
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bioRxiv - Immunology 2024Quote: ... the cells were incubated with fluorescently-labelled antibodies in the presence of anti-CD16/CD32 Fc blocking antibody (2.5 μg/mL, Biolegend, San Diego, CA, USA) for 30 min at 4 °C ...
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bioRxiv - Cell Biology 2024Quote: ... β-III-tubulin antibodies TUJ1 and TUBB3 (801202 and 802001) as well as neurofilament monoclonal antibody SMI312 (837904) were from BioLegend (San Diego, CA). GFP antibody (12A6 ...
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bioRxiv - Immunology 2024Quote: ... Cells were resuspended by mild vortexing and 50 ul of 2.5 µg/ml final (meaning 1/200 dilution of the stock) Fc-block (anti-mouse anti-CD16/32 antibodies, TruStain FcX from Biolegend, San Diego, CA) were added with the following addition of 1/200 dilution of antibodies for surface marker staining and Aqua Live/Dead dye (Thermo Fisher Scientific ...
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bioRxiv - Synthetic Biology 2024Quote: ... 1/20 for secondary mouse anti-human Ig light chain K antibody Alexa Fluor® 647 (316514, BioLegend®, San Diego, CA, USA) and mixed with Hoescht 33342 (H3570 ...
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bioRxiv - Immunology 2024Quote: ... and Propidium Iodide (PI) (1 μg/ml) according to the manufacturer’s instructions (FITC Annexin V Apoptosis Detection Kit with PI, BioLegend, San Diego, CA, USA). The staining of Annexin V requires the usage of a binding buffer for Annexin V as a staining buffer ...
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bioRxiv - Cancer Biology 2024Quote: The isolated B cells (1 x 106 cells) were incubated in prewarmed (37 °C) 1x PBS with 0.5 μM CFSE (Biolegend, San Diego, CA, USA) at 37 °C in a 5% CO₂ incubator for 10 minutes ...
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bioRxiv - Cancer Biology 2021Quote: ... Cells were then stained in FC buffer (2% FCS in PBS) with anti-CD3-APC conjugated antibodies (1:80; BioLegend) and LIVE/DEAD Fixable Near-IR Dead Cell Stain (1:1000 ...
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bioRxiv - Immunology 2021Quote: ... CD18 (clone CBR LFA-1/2, 366310), ITGB7 (clone FIB504, 121006), and CD68 (clone Y1/82A, 333814) were acquired from Biolegend.
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bioRxiv - Immunology 2021Quote: ... containing the following fluorescently-labelled antibodies diluted in FACS buffer (2% FBS in PBS): CD4-APC-Cy7 (clone A161A1; BioLegend), CD8a-Alexa700 (clone SK1 ...
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bioRxiv - Immunology 2021Quote: 1-2 × 106 fresh decidual leukocytes were washed with PBS and stained using the following cocktail of antibodies: CD45 (HI30, Biolegend), CD66b (G10F5 ...
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bioRxiv - Immunology 2021Quote: ... The coronavirus protein panel consisted of the following flurorescent probes: SARS-CoV-2 RBD-APC/Cy7 (streptavidin-APC/Cy7 from BioLegend), WIV1 RBD-BV650 (streptavidin-BV650 from BioLegend) ...
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bioRxiv - Immunology 2021Quote: ... cells were washed in 1xPBS supplemented with 0.5% bovine serum albumin (BSA) and 2 mM EDTA (FACS buffer) and stained with Zombie Aqua Fixable Viability kit (Biolegend) and incubated for 20 min at 4°C followed by surface staining for 30 min at 4°C ...
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bioRxiv - Immunology 2021Quote: ... centrifuged at 200g for 2 minutes followed by ice-cold PBS and incubated with Zombie NIR Fixable Viability dye (Biolegend) to exclude dead cells for 20 minutes at 4 °C ...
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bioRxiv - Neuroscience 2020Quote: ... Permeabilization was followed with blocking step by incubating the samples for 2 hr in PBS containing 3% normal goat serum (NGS, 927503, BioLegend) and 3% BSA and then stained overnight at 4°C with the primary monoclonal antibody (mouse anti-radixin ...
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bioRxiv - Immunology 2021Quote: ... Tonsillar IgD+ B cells were isolated from human tonsil cells by positive selection using biotin-anti-human IgD mAb (clone IA6-2; 348212, Biolegend) and MagniSort™ Streptavidin Positive Selection Beads (MSPB-6003-74 ...
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bioRxiv - Cancer Biology 2021Quote: ... Cells suspensions of 100 μL were incubated for 15 min at RT with 2 μL Human TruStain FcX (Biolegend, 422301) to prevent nonspecific Fc receptor binding ...
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bioRxiv - Bioengineering 2022Quote: ... Splenic cells at 1 × 106 were co-cultured with target cells at 1:2 effector to target ratio in the presence of anti-CD107a (Biolegend), brefeldin A and monensin and a serial dilutions (6x ...
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bioRxiv - Molecular Biology 2022Quote: ... or DDX39B-depleted (Sh3 and Sh5) MT-2 cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set (BioLegend). The permeabilized cells were stained with antibodies against FOXP3 conjugated with Alexa Fluor 647 (AB_2539534 ...
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bioRxiv - Immunology 2022Quote: ... The plates were washed twice with PBS plus 2 % FBS and subsequently coated with 10 μg/ml unconjugated anti-CD16 (3GB, BioLegend) in PBS overnight at 4°C ...
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bioRxiv - Biochemistry 2022Quote: ... Cells were then washed once with ice-cold FACS buffer and incubated with 2 μg/mL PE anti-human IgG Fc (BioLegend) at 4 °C with gentle shaking in the dark for 1 hour ...
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bioRxiv - Immunology 2022Quote: ... PBMC derived T cells were stimulated for five days at 37°C with 1μg/mL anti-CD3 and anti-CD28 and 10ng/mL of recombinant human IL-2 and recombinant human IL-12p70 (BioLegend). On day four of stimulation ...
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bioRxiv - Immunology 2020Quote: ... Supernatant from eleven donors responding in the ELISPOT assay were profiled using a 12-plex Legendplex T Helper Cytokine Panel Version 2 (Biolegend) following the manufactures instructions ...
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bioRxiv - Immunology 2020Quote: Recipient mice were infused with 2-3 x 106 purified donor T cells that were labeled with 5 μM CFSE (BioLegend) as previously described63 ...
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bioRxiv - Immunology 2020Quote: ... at a total concentration of 5×106 cells/mL in RPMI 1640 + 10% FBS + 1% Pen/Strep +50U/mL IL-2 (R10-50) with 1/100 anti-CD107a-PE antibody (Biolegend) and 1/1,000 Monensin GolgiStop™ (BD) ...
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bioRxiv - Microbiology 2021Quote: ... and incubated with anti-CoV-2 Spike RBD mAb (SARS2-02) (13) followed by APC-conjugated goat anti-mouse IgG antibody (Cat# 405308, BioLegend). For surface detection ...
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bioRxiv - Cancer Biology 2021Quote: ... cells were activated for 24h using 2 μg/ml of anti-Cd28 and anti-Cd3ε antibodies (102116 and 100340, BioLegend).
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bioRxiv - Immunology 2021Quote: ... The panel for the cohort that received the SARS-CoV-2 spike prime followed by the RBD homotrimer cocktail boost also included SARS-CoV RBD-APC (streptavidin-APC from BioLegend) and WIV1-CoV RBD-BV650 (streptavidin-BV650 from BioLegend) ...
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bioRxiv - Microbiology 2020Quote: ... After incubation the cells were washed twice in FACS buffer (PBS, 0.5% BSA, 2 mM EDTA) and stained with a CD19-APC antibody (BioLegend # 302212) to analyze pDCs for 20 min at 4°C to analyze B cells or with two antibodies directed against CD303(BDCA2)-APC (Miltenyi Biotec ...
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bioRxiv - Immunology 2020Quote: ... Cocktail 2 was used to identify DE cells and contained 3 μl of anti-human CD5-APCeF780 antibody (clone L17F12, Biolegend), 2 μl of anti-human CD19-PECy7 antibody (clone HIB19 ...
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bioRxiv - Immunology 2020Quote: ... Cells were stained at 4°C for 30 min in Hanks Balanced Salt Solution-2% FBS with the following markers: anti-THY1 AF700 1:100 (Biolegend), anti-PDGFRalpha PE 1:100 (Biolegend ...
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bioRxiv - Immunology 2022Quote: ... Cells were prepared for flow cytometry by washing once with PBS containing 2% HI-FBS followed by incubation with Brilliant Violet 421 anti-human IgG Fc Antibody (clone M1310G05) (Biolegend) or Brilliant Violet 421 Streptavidin (BioLegend ...
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bioRxiv - Immunology 2022Quote: ... Biotinylated full-length SARS-CoV-2 Spike protein was purchased from Acro Biosystems and SARS-CoV-2 Spike protein Receptor-Binding Domain (RBD) was purchased from BioLegend.
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bioRxiv - Immunology 2022Quote: The collected BMDCs were centrifuged once and resuspended in cell staining buffer (PBS supplemented with 2% FBS) containing anti-Fc receptor mAb (Biolegend). Cells were blocked for 10 min at 4°C ...
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bioRxiv - Immunology 2022Quote: ... TCR stimulation was evaluated by measuring IL-2 concentration in culture supernatant using an IL2 ELISA kit (#423001, #423501, #431001 and #421101, BioLegend).
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bioRxiv - Immunology 2022Quote: ... Cells were plated in a 24-well plate at a concentration of 500,000 cells/mL on either plate-bound α-CD3ε (2 μg/mL; 145-2C11; BioLegend) and soluble α-CD28 (1 μg/mL ...
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bioRxiv - Immunology 2022Quote: ... Sorted 2D2 CD4+ T cells were activated with plate-coated 2 μg/mL anti-mouse CD3e mAb (clone 145-2C11, BioLegend), soluble 1 μg/mL anti-mouse CD28 mAb (clone 37.51 ...
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bioRxiv - Immunology 2020Quote: ... fixed with 2% formaldehyde and permealized with BD Perm/Wash buffer (Fischer) and stained with anti IFN-γ (clone XMG1.2; BioLegend) and anti-GM-CSF (clone MP1-22E9 ...