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Citations for BioLegend :
2151 - 2200 of 2559 citations for TD 5108 CAS 866933 46 2 99% since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2020Quote: ... stained for 20 min at 4 °C in PBS/2% FCS in presence of Fc receptor blocking reagent (Biolegend) with anti-murine CD326-BV605 ...
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bioRxiv - Immunology 2021Quote: ... with the caveat that CD8+ enrichment was achieved by incubating 1-2 million PBMCs with CD8 Mojobeads (Biolegend 480108) for 15 minutes on ice ...
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bioRxiv - Cancer Biology 2022Quote: ... washed in blocking solution (2% BSA in PBS) and immunolabeled with anti-CD24 PerCP/Cy5.5 and anti-CD44 FITC primary antibodies (BioLegend, 1:50 ...
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bioRxiv - Immunology 2022Quote: ... for 30mins at 37°C, washed, resuspended in FACS buffer (PBS, 2% FBS) and co-stained with anti-CD4 (GK1.5, Biolegend), anti-CD62L (MEL-14 ...
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bioRxiv - Bioengineering 2022Quote: ... 100 IU/mL recombinant human IL-2 (Proleukin, Prometheus Laboratories) and 5 ng/mL recombinant human IL-7 (BioLegend) at 37C ...
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bioRxiv - Immunology 2022Quote: ... enriched CD8 T cells were incubated for 10’ at 4°C in Staining buffer (2%BSA/0.01% Tween in PBS) containing FcBlock (1/10 dilution ;TruStainTM FcX, Biolegend) followed by a 30’ incubation in Staining Buffer containing TotalSeqB antibodies against Ly108 ...
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bioRxiv - Immunology 2022Quote: ... and nonspecific staining was then blocked by addition of anti-CD16/32 (2 μg/ml; mouse TrueStain FcX, BioLegend), 2% rat serum (Invitrogen) ...
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bioRxiv - Immunology 2022Quote: ... CD19+ B cells were stimulated for 24 hours using Purified F(ab’)2 Goat anti-mouse IgM Antibody (BioLegend). CD14+ monocytes were stimulated for 24 hours with 1ug/ml of LPS ...
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bioRxiv - Microbiology 2022Quote: ... then retro-orbitally injected with 2 μg/mouse of APC anti-mouse CD45 (Biolegend, cat#103112; clone 30-F11) antibody in 50 μL PBS for intravenous labeling of CD45+ circulating lymphocytes 15 minutes prior to euthanization ...
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bioRxiv - Immunology 2022Quote: ... SARS-CoV-2 biotinylated RBD protein (His & AVI Tag) (SinoBiological, 40592-V27H-B) conjugated to APC-streptavidin (BioLegend, 405207). After washed twice ...
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bioRxiv - Cell Biology 2022Quote: ... Cells were then washed with suspension buffer (PBS + 0.5% BSA + 2 mM EDTA + 4.5 mg/mL D-glucose) and resuspended in RBC Lysis Buffer (Biolegend 420301) for one minute at room temperature to remove red blood cells ...
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bioRxiv - Cell Biology 2024Quote: ... Membranes were blocked overnight in Odyssey PBS Blocking Buffer (Li-Cor) and stained for 2 hours at RT with Syn1 (1:1,000; Biolegend) or γH2AX (1:1,000 ...
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bioRxiv - Genomics 2024Quote: ... at a ratio of 1:1 (cell:Dynabead) in the presence of 5ng/mL rhIL-2 and 10ng/mL rhIL-7 (Biolegend) in 96 well U-bottom plates ...
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bioRxiv - Immunology 2024Quote: ... then after 2 washes cells were incubated with streptavidin (SA) conjugated to Brilliant Violet 650 (BV650, 1:200, BioLegend) in PBS on ice for 15 minutes ...
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bioRxiv - Microbiology 2023Quote: ... MBL) or H-2q-restricted LCMV NP118 peptide (MBL) and 50 U/mL of recombinant mouse IL-2 (BioLegend) for 1 h ...
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bioRxiv - Cell Biology 2023Quote: ... approximately 200,000 cells per sample were resuspended in staining buffer (PBS, 2% BSA, and 0.01% Tween) and incubated for 10 minutes with Fc block (TruStain FcX (BioLegend) and FcR blocking reagent (Miltenyi) ...
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bioRxiv - Immunology 2023Quote: ... cells were stained for sorting in PBS containing 2% FCS at 4 °C with the following antibodies: CD271-PE (1:200; ME20.4, BioLegend), CD235a-PECy7 (1:20 ...
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bioRxiv - Immunology 2023Quote: ... and resuspended in FACS buffer (PBS with 0.5% BSA and 2 mM EDTA) with Fc blocked using human TrueStain FcX (Biolegend) and incubated in the dark for 10 min at room temperature ...
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bioRxiv - Bioengineering 2023Quote: ... cells were washed with FACS buffer (PBS + 2% FBS + 0.05% NaN3) and incubated with Fc receptor blocking solution (Human TruStain FcX, BioLegend) for 5 minutes ...
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bioRxiv - Immunology 2023Quote: ... Cells were subsequently resuspended in staining buffer (PBS with 0.5% bovine serum albumin and 2 mM EDTA) and Fc-blocked using TruStain FcX reagent (BioLegend). Cells were then stained with the following fluorophore-conjugated anti-mouse antibodies for 30 min at 4°C ...
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bioRxiv - Immunology 2023Quote: ... cells were resuspended in FACS buffer (PBS with 0.5% BSA and 2 mM EDTA) and Fc blocked using human TrueStain FcX (Biolegend). For cell surface staining ...
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bioRxiv - Neuroscience 2023Quote: ... Slides were incubated in primary antibody in 0.1 M Tris/2% FBS in a humidified chamber overnight at 4°C (SMI-32, Biolegend 801702 ...
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bioRxiv - Immunology 2023Quote: Surface staining for flow cytometry analysis was performed in PBS supplemented with 2% FBS and Trustain FcX (Biolegend, 422301) at room temperature ...
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bioRxiv - Immunology 2023Quote: ... Enriched CellTrace Violet-labeled OT-I CD8+ T cells were resuspended at 20,000 cells/100uL in complete RPMI + 10ng/mL of recombinant mouse IL-2 (Biolegend). As positive control ...
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bioRxiv - Biochemistry 2024Quote: ... We determined the amounts of IL-2 in the culture supernatant of activated or naïve CD4+ T cells with an ELISA kit (BioLegend). We measured the amounts of IgE in the serum with a commercially available ELISA kit (Sigma-Aldrich).
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bioRxiv - Immunology 2024Quote: ... lymph node suspension from 2 mice per biological replicate were activated with 0.5 µg/mL anti-mouse CD3 (Biolegend) and 0.5 µg/mL anti-mouse CD28 (eBioscience ...
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bioRxiv - Immunology 2024Quote: ... the supernatant was discarded and the cell pellet was resuspended in 50 µL of FACS Buffer (0.5% BSA, 2 mM EDTA in DPBS) with 1 µL of human TruStain Fc-block (1:50, Biolegend) and incubated for 15 minutes at 4 °C ...
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bioRxiv - Microbiology 2024Quote: ... supplemented with recombinant IL-2 (R&D Systemts) at 10ng/mL and 4µg/mL of anti-CD28 antibody (Biolegend), plated in 24-well plates coated with 2µg/mL of anti-CD3 antibody (Biolegend) ...
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bioRxiv - Immunology 2024Quote: ... Cells were washed in 2% FBS 2 mM EDTA-PBS and surface stained with the following anti-human antibodies: CD45 (PerCP/Cyanine5.5, 2D1, Biolegend), CD137 (BV650 ...
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bioRxiv - Immunology 2024Quote: ... or 96-well plates precoated overnight at 4°C with 2 µg/ml anti-CD3ε antibody (145-2C11, BioLegend). For Th0 and Th17 differentiation ...
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bioRxiv - Immunology 2024Quote: ... resuspended in 100ul of PBS with 10% FBS and 2 μl of anti-human CD3 AF488 (clone UCHT1, Biolegend), 2 μl of anti-human CD14 BV480 (clone M5E2 ...
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bioRxiv - Immunology 2024Quote: ... followed by incubation with the secondary antibody PE F(ab’)2 Goat anti-human IgG Fcγ (BioLegend, Cat 398004) for 1h at room temperature ...
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bioRxiv - Immunology 2022Quote: ... LEAF Purified anti-mouse CD3ε Antibody (100313) and LEAF Purified anti-mouse CD28 Antibody (102111) were purchased from Biolegend (San Diego, CA, USA). Staphylococcus Entrotoxin B (BT202 ...
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bioRxiv - Neuroscience 2021Quote: ... The cell pellets were washed once with 15 mL PBS and resuspended in 1 mL of red blood cell (RBC) lysis buffer (BioLegend, San Diego, CA) for 2 min at room temperature to lyse RBCs ...
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bioRxiv - Biophysics 2022Quote: ... coverslips of each batch were incubated with 30 µg/ml anti-human IgG Fc (cross-reacts with rabbit) conjugated to Alexa Fluor-488 (Cat# 409321; BioLegend, San Diego, CA) for 45 minutes at room temperature in the dark ...
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bioRxiv - Immunology 2022Quote: ... Cells were washed with FACs buffer (1X PBS, 1% FBS) and fixed at room temperature for 20 minutes with Intracellular Staining Fixation Buffer (BioLegend, San Diego, CA). Cells were washed with 1X Cyto-Fast™ Perm Wash solution (BioLegend ...
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bioRxiv - Immunology 2022Quote: ... TF and ICS was performed following permeabilization using True-Nuclear buffer kit according to the recommended protocol (True-Nuclear Transcription Factor Buffer Set; BioLegend, San Diego, CA). Samples were acquired on a Cytek Aurora (Cytek ...
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bioRxiv - Immunology 2020Quote: ... We also isolated plasma from GVHD patients and healthy donors and performed cytokine ELISAs on these plasma samples using multiplex ELISA kits (Biolegend, San Diego, CA).
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bioRxiv - Immunology 2021Quote: ... anti-CD4 [OKT4], anti-CD8a [RPA-T8], anti-CD25 [M-A251], anti-CD45RA [HI100], and anti-CD127 [A019D5], all from BioLegend, San Diego, CA). Pilot experiments comparing FACS sorting and magnetic bead separation showed similar results with respect to cell purity ...
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bioRxiv - Bioengineering 2021Quote: ... Anti-FLAG epitope (DYKDDDDK, catalog# 637301) and horseradish peroxidase (HRP) Donkey anti-human IgG antibody (catalog #410902) were purchased from Biolegend (San Diego, CA). The secondary antibody anti-rat IgG HRP (catalog# 7077 ...
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bioRxiv - Neuroscience 2021Quote: ... FCS files were evaluated with the LEGENDplex™ Data Analysis Software Suite using the Qognit cloud base platform (Biolegend, San Diego, CA, USA). A five-parameter logistic curve-fitting method was used to calculate concentrations from Median Fluorescence Intensity and normalized to the amount of protein in each sample ...
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bioRxiv - Cancer Biology 2020Quote: ... Alexa Fluor 488 anti-human CD206 and PE/Cy7 anti-human Interleukin-10 (IL-10) were used for analysis (all from BioLegend, San Diego, CA). The samples were run on an LSR Fortessa cell analyzer from BD (Becton Dickinson ...
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bioRxiv - Cancer Biology 2020Quote: ... CD4+ T cells were isolated from the PBMC population by immunomagnetic positive selection using the MojoSort Human CD4 Nanobeads (BioLegend, San Diego, CA) and an EasySep Magnet (STEMCELL™ Technologies Inc. ...
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bioRxiv - Bioengineering 2021Quote: ... 22 and 28 before differentiation of mast cells was assessed by flow cytometry with antibody markers for CD117 APC (2B8, Biolegend, San Diego, CA) and FcεRI PE (MAR-1 ...
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bioRxiv - Microbiology 2020Quote: ... After 48h of co-infection the cells were harvested and processed as above using an anti-MHC-II FITC-conjugated antibody (Biolegend San Diego, CA). Treatment with PMA (50 ng/ml ...
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bioRxiv - Microbiology 2020Quote: ... We aspirated pelleted barcoded cells to a volume of 50 µL and incubated them with 15 µL of Human TruStain FcX (Biolegend, San Diego, CA) for 10 minutes ...
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bioRxiv - Microbiology 2020Quote: ... Supernatants from triplicate wells of each condition were collected at 24 hrs and assayed for the IL-8 concentration using a human IL-8 enzyme-linked immunosorbent assay (ELISA) kit according to the instructions of the manufacturer (BioLegend, San Diego, CA). IL-8 values were reported as normalized values defined as a proportion increased or decreased compared to values obtained for J99 ...
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bioRxiv - Microbiology 2021Quote: ... 100 µl containing 1 × 106 cells in PBS were incubated with yellow Zombie viability dye (1:1000 dilution, Cat. No 423104, Biolegend, San Diego, CA) for 15 minutes at room temperature followed by washing in FACS buffer ...
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bioRxiv - Neuroscience 2020Quote: ... in PBS and then incubated with an Fc receptor blocking solution (Human TruStain FcX, 1:20 in staining buffer; Biolegend, San Diego, CA) for 10 min ...
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bioRxiv - Microbiology 2021Quote: ... incubated 15 minutes on ice and then stained at RT in the dark for 20 minutes with the following mixture of fluorescent-labeled antibodies: CD3-APC (Biolegend, San Diego, CA), CD4-BV510 (Biolegend ...