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Citations for BioLegend :
1951 - 2000 of 2016 citations for FITC labeled Fibrinogen since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2023Quote: ... Cells were collected and stained with fluorescently labeled antibodies against CD45 PE-Cy 7 (clone: 30-F11, cat # 103114: Biolegend), TCRβ APC (clone ...
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bioRxiv - Immunology 2023Quote: ... THP-1 and SKOV-3 cell lines and primary human T cells were labeled with fluorescent antibody against human CD70 or isotype control (Biolegend) to verify antigen expression as described before35.
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bioRxiv - Immunology 2023Quote: ... Cells were centrifuged at 400 g for 8 minutes and stained with lanthanide-labeled antibodies against immunophenotypic markers in CyTOF buffer containing Fc receptor blocker (BioLegend) for 30 minutes at room temperature (RT) ...
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bioRxiv - Bioengineering 2023Quote: ... The expression of Mo surface markers was measured by flow cytometric analysis using fluorescently labeled antibodies against CD11b (BioLegend #101207), CD45R (BioLegend #103211) ...
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bioRxiv - Immunology 2023Quote: ... Lung resident cells were distinguished from those in the vasculature by exclusion from staining with fluorescently labeled anti-CD45 antibody (PerCP, clone 30F-11, BioLegend) that was injected retro-orbitally into each mouse approximately 3 minutes prior to euthanasia65 ...
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bioRxiv - Cancer Biology 2023Quote: ... 5 x105 cells were labeled with 1 μg anti-hCD81-PE clone 5A6 or 1 µg isotype control-PE (349506, 400114; Biolegend)32 and by Western blotting (4-12% SDS-PAGE ...
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bioRxiv - Physiology 2023Quote: THP-1 monocytes (2×105 cells/mL) were labeled with 5µM cell-permeant Calcein-AM in DMSO (1:200, BioLegend, 425201) for 20 minutes at 37°C ...
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bioRxiv - Immunology 2023Quote: Biotinylated protein antigens were individually multimerized with fluorescently labeled streptavidin (BB515, BD Biosciences; BUV615, BD Biosciences; AF647, Biolegend; BV421, Biolegend) as described previously (25) ...
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bioRxiv - Immunology 2023Quote: Biotinylated protein antigens were individually multimerized with fluorescently labeled streptavidin (BB515, BD Biosciences; BUV615, BD Biosciences; AF647, Biolegend; BV421, Biolegend) as described previously (25) ...
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bioRxiv - Immunology 2024Quote: A separate tube of the digested cell suspension was labeled for flow cytometry analysis of immune cells and stained with Zombie Violet (Biolegend) as well as CD45-FITC [clone HI30] ...
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bioRxiv - Immunology 2024Quote: ... cells were labeled stained in PBS for 20 mins at 4°C with a live dead marker (Zombie UVTM, Biolegend). Surface staining was performed in brilliant stain buffer (BD Biosciences ...
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bioRxiv - Immunology 2024Quote: ... were stained on ice for 25 minutes with the following panel of lineage-specific antibodies: Biotin-labeled TER-119 (TER-119, BioLegend), Biotin-labeled GR-1 (RB6-8C5 ...
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bioRxiv - Immunology 2024Quote: ... samples were incubated overnight at 4°C with combinations of the following antibodies: PE-Cy7-labeled CD63 (NVG-2, BioLegend), AF532-labeled iNOS (CXNFT ...
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bioRxiv - Immunology 2024Quote: ... CD8+ T-cells from the mouse ear were sorted with a FACSAria sorter from samples treated with BHI-tween or HSV-1 and labeled with TotalSeqC hashtags antibodies (Biolegend). All samples were pooled together ...
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bioRxiv - Immunology 2024Quote: ... Fluorescently labeled anti-mouse antibodies against CD45 (104) and H-2Kb/H-2Db (28-8-6) were obtained from BioLegend.
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bioRxiv - Immunology 2024Quote: PBMCs (2×107) of HFRS patients were stained with PE-Cy7-labeled anti-human CD3 mAb (Clone 17A2, BioLegend, USA), APC-Cy7-labeled anti-human CD56 mAb (Clone 5.1H11 ...
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bioRxiv - Physiology 2024Quote: ... The cell suspensions were treated with the fluorescent dye-conjugated antibodies and were labeled with Zombie Aqua Viability Dye (BioLegend). Then the cell suspensions were fixed with 4% paraformaldehyde ...
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bioRxiv - Immunology 2024Quote: ... peptide-pulsed K562/HLA-E cells were stained with APC-labeled anti-human HLA-E mAb (clone 3D12, BioLegend, USA). Then the CD94/NKG2A+HLA-E+ and CD94/NKG2C+HLA-E+ K562/HLA-E cells were gated as targeted cells.
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bioRxiv - Bioengineering 2024Quote: ... Surface staining was carried out using a fluorochrome-labeled PE-conjugated anti-human CD11a (HI111) monoclonal antibody obtained from BioLegend.
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bioRxiv - Cell Biology 2024Quote: ... hemolyzed lineage-positive and Sca-1-positive cells were labeled with biotin-conjugated antibodies and removed using MojoSort streptavidin nanobeads (BioLegend) according to the manufacturer’s instruction ...
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bioRxiv - Physiology 2020Quote: ... and MDA-MB-468/sh cells were grown and subjected to incubation with fluorescein isothiocyanate (FITC)-conjugated anti-CD24 (Cat. #311104; BioLegend, San Diego, CA, USA) and PE-conjugated anti-CD44 (Cat ...
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bioRxiv - Genomics 2020Quote: ... cells were resuspended in PBS complemented with 2% fetal bovine serum and stained with FITC anti-human CD45 antibody (BioLegend #304006CD45, 1:200 dilution) and PE anti-human EPCAM antibody (Miltenyi Biotech #130-113-264 ...
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bioRxiv - Immunology 2022Quote: ... Samples were washed in FACS buffer to remove unbound tetramer prior to surface staining with anti-TCR□β APC (clone IP26) and anti–CD7 FITC (clone CD7-6B7, Biolegend, San Diego, CA) for 30 minutes at 4°C ...
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bioRxiv - Biochemistry 2024Quote: ... An additional 30uL of 1× TFP Perm/Wash buffer was added containing the following antibodies: CD3 FITC (UCHT1, BioLegend 300406, 0.2 test volume/well), CD4 BV421 (SK3 BioLegend 344632 ...
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bioRxiv - Developmental Biology 2024Quote: ... The cells were pelleted and resuspended in FACS buffer for staining with the following antibodies for 20 min at 4° C: Ter119-FITC (BioLegend, Catalog No. 116205, 1/100), EpCAM-PE (BioLegend ...
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bioRxiv - Genomics 2024Quote: ... Antibodies were added to stain surface markers for 30 min at 4°C (CD3 PE, Life Technologies; CD8 FITC, Life Technologies; CD11b AF700, Life Technologies CD19 PerCP-Cy5.5, BioLegend; C33 BV605, BioLegend; CD56 BV711, BD). After 30min ...
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bioRxiv - Bioengineering 2024Quote: The following anti-human antibodies were used in the PBMC staining panel for NK cells: (donors 1-2) anti-IFN-γ FITC (BioLegend catalog #506504, clone B27), anti-CD19 PerCP/Cy5.5 (BioLegend catalog #309504 ...
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bioRxiv - Molecular Biology 2020Quote: Freshly isolated CD3+ T cells were labeled using CFSE working solution (5 μM) using the CFSE Division Tracker Kit (Biolegend, USA). T cells were incubated for 20 min at 37 ◻ and quenched staining with culture medium ...
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bioRxiv - Molecular Biology 2021Quote: ... incubated with an Alexa Fluor 647-labeled mouse monoclonal antibody against the human IgG1-Fc (Biolegend, San Diego, USA, cat #409320) and analyzed in a Gallios flow cytometer (Beckman Coulter ...
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bioRxiv - Immunology 2022Quote: ... Cells from each strain were then labeled with TotalSeq™-A0301 anti-mouse Hashtag Antibody against CD45 and MHC-I (BioLegend, Clone M1/42 and 30-F11 ...
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bioRxiv - Cell Biology 2022Quote: ... The cells were then stained with a PE-labeled anti-huCD4 antibody (SK3 clone, Cat#344606, BioLegend, San Diego, CA, USA) and incubated in the dark for 45 min at 4°C ...
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bioRxiv - Developmental Biology 2024Quote: Single-cell suspensions were obtained at day 2.5 of differentiation Cells were first labeled with Zombie NIR fixable viability dye (BioLegend, 1:500) for the exclusion of dead cells ...
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bioRxiv - Microbiology 2023Quote: ... the cells were incubated with Alexa Fluor® 488-labeled anti-human CD69 antibody or an isotype control (1 µg/ml, BioLegend) for 45 min ...
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bioRxiv - Immunology 2023Quote: ... Ex vivo CD8+ T cells or in vitro stimulated (IVS) cultures were labeled with the Zombie Aqua™ Fixable Viability Kit (BioLegend) to exclude dead cells in all flow cytometry experiments ...
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bioRxiv - Cancer Biology 2023Quote: ... Later 25µl of the following mixture of fluorescent-labeled antibodies was added to the samples: CD3-APC (Biolegend, San Diego, CA), CD4-BV510 (Biolegend ...
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bioRxiv - Cancer Biology 2024Quote: Output of CD34+ cells following hematopoietic differentiation of hiPSCs was determined by staining with PE/Dazzle594-labeled anti-human CD34 antibodies (BioLegend, #343534). Prior to addition of antibodies ...
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bioRxiv - Biochemistry 2024Quote: ... cells were collected and analyzed by flow cytometry for CTV-labeled populations along with the following: APC anti-CD8a (clone 53-6.7, BioLegend Cat# 100712), Brilliant Violet 711 anti-CD44 (clone IM7 ...
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bioRxiv - Cancer Biology 2024Quote: ... single cells isolated from tissue were labeled for 30 min at room temperature with Alexa Fluor 647-conjugated anti-EpCAM (1:50, Biolegend 324212), PE-conjugated anti-CD49f (1:100 ...
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bioRxiv - Immunology 2024Quote: ... The expression of HLA-E on the surface of K562/HLA-E cells was then determined by staining with APC-labeled anti-human HLA-E mAb (clone 3D12, BioLegend, USA) and detected by ACEA NovoExpress system (Agilent Technologies ...
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bioRxiv - Immunology 2024Quote: ... after which cell death of Cell Trace Yellow (CTY) labeled RMA-S cells was assessed by flow cytometry according to Annexin V (Biolegend 640920) expression.
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bioRxiv - Neuroscience 2022Quote: ... Cells were washed and then incubated with conjugated antibodies (FITC-CX3CR1, #149020 or AlexaFluor488-CD115, #135512; PE-P2Y12, #848004 or PE-CD68, #137014; Biolegend; PerCp-Cy5-CD11b; #550993; PE(594)-CD45 ...
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bioRxiv - Neuroscience 2021Quote: Nrxn1 neuron cKO-T brain slices (coronal and sagittal) were labeled for Nrxn1 (Fig. 1c-d) using purified HA-647 (mouse, 1:500, Biolegend, Cat# 682404) as described above ...
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bioRxiv - Cell Biology 2022Quote: ... of the sorted MBS-MEF and MCF7 cell populations was then counterstained with Alexa-488-labeled anti-human CD326 (Biolegend; Cat #: 324210) and PE-labeled anti-mouse CD321 antibody (BD Biosciences ...
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bioRxiv - Immunology 2020Quote: ... Absolute calibration of pMHC surface density was done as previously described by labeling with an excess of Alexa Fluor 488 labeled anti-HLA antibody (Biolegend, San Diego) and fluorescence determination.
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bioRxiv - Immunology 2020Quote: ... Cells were subsequently washed twice with PBSF and labeled with 50 μL of APC-conjugated monoclonal mouse anti-hemagglutinin tag (HA).11 antibody (BioLegend, Cat # 901524), PE-conjugated goat anti-human IgG polyclonal antibodies (Southern Biotech ...
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bioRxiv - Plant Biology 2021Quote: ... Biotin labeled DNA was detected with 1:5000 solution of an anti-biotin HPR-conjugated antibody (BioLegend, San Diego, California, United States) in TBST with 5% BSA ...
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bioRxiv - Immunology 2024Quote: ... Primary human CD4+ T cells from CTLA4-deficient patients or healthy controls were purified from 5 million frozen PBMCs using a biotin labeled anti-human CD4 antibody (OKT4; BioLegend; 1:20) followed by magnetic separation with anti-biotin microbeads (Miltenyi Biotic ...
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bioRxiv - Immunology 2022Quote: Human single-cell suspensions isolated from the spleen or BM of xenografted mice were processed and labeled as above were the following antibodies: CD45 PerCp (Biolegend, clone HI30), CD34 BUV615 (BD bioscience ...
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bioRxiv - Synthetic Biology 2023Quote: ... OD600 of induced cultures was taken and used to determine approximate volumes of culture to stain two million cells with fluorescently labeled antibodies (anti-FLAG PE, Biolegend, cat# 637309, anti-HA Alexa 647, Biolegend, cat #682404). Cells were washed in PBS 0.5% BSA (0.5% BSA ...
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bioRxiv - Cancer Biology 2023Quote: ... we triple-washed samples with 0.1% PBS-Tw and labeled them with streptavidin-BV510 (Biolegend, 405234; 1% BSA in 0.1% PBS-Tw). After a 30-min incubation ...