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Citations for BioLegend :
1851 - 1900 of 2016 citations for FITC labeled Fibrinogen since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2023Quote: ... The cells were washed with cold PBS and PE-labeled anti-human IgG Fc secondary antibody (Biolegend, 41070) was added and incubated for 30 min at 4°C ...
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bioRxiv - Immunology 2023Quote: ... or MCC:I-Ek (cognate) tethered pMHCII complexes were labeled with Tag-it Violet according to the manufacturer’s instructions (BioLegend). M12 cells and 58α-β- cells were then chilled on ice for 30 minutes ...
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bioRxiv - Immunology 2024Quote: ... and then stained for 30 minutes on ice with an APC-labeled antibody against CD3 (BioLegend; clone UCHT1), phycoerythrinCy7–labeled (PE-Cy7–labeled ...
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bioRxiv - Immunology 2022Quote: ... The samples purified in this way from each group of recipients were then suspended in 100μL buffer and labeled with 1μg per sample of the following Total-seq A antibodies from BioLegend: TotalSeq™-A0198 anti-mouse CD127 (A7R34) ...
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bioRxiv - Developmental Biology 2023Quote: ... then for 1 hour at room temperature (RT) with biotinylated secondary antibodies and finally with fluorochrome-labeled (BioLegend) or peroxidase-labeled streptavidin (Beckman Coulter) ...
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bioRxiv - Cancer Biology 2023Quote: ... transgenic T cells were labeled with Tag-it Violet Proliferation and Cell Tracking Dye (Cat No. 425101 Biolegend). The frequency of SMARTA cells that proliferated during culture was determined as described by Gett et al ...
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bioRxiv - Immunology 2024Quote: ... 5 μl PE-labeled mouse anti-human CD11b fluorescent antibody (cat. no. 301306; 1:1,000 dilution; BioLegend, Inc.) or FITC-labeled mouse anti human CD14 fluorescent antibody (cat ...
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bioRxiv - Immunology 2024Quote: ... and immunofluorescently labeled with combinations of the following specific antibodies: Alexa Fluor 488 CD11b (Biolegend; clone M1/70), Alexa Fluor 594 Ly6G (Biolegend ...
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bioRxiv - Cancer Biology 2021Quote: ... The cells and the culture media were collected and stained with annexin V-FITC mAb (BioLegend, San Diego, CA, Cat. No. 640906) in binding buffer (10 mM HEPES ...
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bioRxiv - Biochemistry 2020Quote: ... incubated with secondary antibody (1/100 dilution of chicken anti-HIS-FITC polyclonal from Immunology Consultants Laboratory; or 1/250 anti-human IgG-APC clone HP6017 from BioLegend) for 30 minutes on ice ...
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bioRxiv - Immunology 2022Quote: ... anti-B220/CD45R (clone RA3-6B2 in APCeF780 from eBiosciences Cat# 47-0452-82, in AF700 from BD Biosciences Cat# 557957 or in FITC, from BioLegend Cat# 103206); anti-CD45 (clone 30-F11 in PECy7 from eBiosciences Cat# 25-0451-82 or in BV786 ...
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bioRxiv - Immunology 2022Quote: ... anti-NK1.1 (clone PK136 in APCeF780 Cat# 47-5941-82 or APC cat# 17-5941-82 from eBiosciences, in AF700 from BD Biosciences Cat# 560515 or in FITC from BioLegend Cat# 108706); anti-CD11b (clone M1/70 in BV421 ...
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bioRxiv - Microbiology 2021Quote: ... Intracellular P-STAT5 staining was then performed in PBS with wash steps performed at 1800 rpm for 6 min at 4°C.The following antibody clones and fluorochromes were used: CD3 (UCHT1, Biolegend; Bv510, Bv711, FITC), CD8 (SK1 ...
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bioRxiv - Immunology 2022Quote: ... Cells were successively stained for 30 minutes on ice with the cocktail of fluorescently conjugated antibodies in 200 μl staining buffer (1X PBS + 2% FBS): 2 μg/ml biotinylated-RBD-Streptavidin-PE and FITC-anti-human IgG antibody (Biolegend, clone: M1310G05) and DAPI ...
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bioRxiv - Immunology 2022Quote: ... slices were incubated with 20 μg/ml anti-B220 antibodies (AF647-αB220 Rat IgG2a, κ or FITC-αB220 Rat IgG2a, κ, BioLegend) at 37 °C for 1 h ...
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bioRxiv - Immunology 2020Quote: ... Then these PBMCs was incubated with mixed antibodies cocktail at 4 °C for 30 min (the antibodies cocktail including FITC-anti-human CD19 antibody (Biolegend, clone: SJ25C1), BV421-anti-human IgD antibody (Biolegend ...
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bioRxiv - Biophysics 2020Quote: ... The lineage depleted fraction was stained with 13 markers for lineage-specfic monoclonal antibodies FITC (CD2, CD3, CD4, CD11b, CD14, CD15, CD24, CD10, CD41, CD20, CD66c, CD127, CD33) (Biolegend and BD) and Pacific blue conjugated anti-CD34 (Biolegend) ...
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bioRxiv - Immunology 2020Quote: ... the individual antibody hit at 5 μg/ml and/or ACE2-mFc was incubated with 1-2 million 1D8 spike+ cells and detected by anti-human-Fc PE (invitrogen, 12-4998-82) and FITC Goat anti-mouse-IgG (Biolegend, cat#405305) or APC Goat anti-mouse-IgG (Biolegend ...
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bioRxiv - Cell Biology 2023Quote: ... the pellet was resuspended in PBS and incubated for 30 min at 4 °C with rat FITC-conjugated mAb against CD11b (1:200, BioLegend, CA, USA). After incubation ...
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bioRxiv - Developmental Biology 2024Quote: ... The APC depleted fraction was collected and used for staining with the following antibodies: Ter119-FITC (BioLegend, Catalog No. 116205, 1/800), Cd45R-FITC (BioLegend ...
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bioRxiv - Immunology 2024Quote: ... FITC (or APC)-conjugated anti-CD177 mAb (clone MEM-166, mouse IgG1) and respective isotype controls were from BioLegend (San Diego, CA). FITC-conjugated goat anti-human Ig(H+L ...
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bioRxiv - Immunology 2023Quote: ... MALP-like cells were identified as the ADRB2highCD106+ population (using unconjugated ADRB2, Thermofisher, Cat. BS-0947R followed by rabbit IgG-FITC, Abcam, Cat. ab6717 and CD106-PE-Cy7, Biolegend, Cat. 305817). A 5-laser Cytek Aurora machine was used for the analysis.
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bioRxiv - Bioengineering 2022Quote: ... Cells were stained for 30min in flow buffer (PBS, 1mM EDTA, 2% FBS) with antibodies against CD8α-FITC (53-6.7; Biolegend unless otherwise noted), CD19-PerCP/Cy5.5 (1D3) ...
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bioRxiv - Immunology 2022Quote: ... each sample was pelleted at 1,150 x g in a centrifuge for 5 min at room temperature and then stained with 50 µL of an antibody cocktail containing 1:100 diluted anti-human CD3-FITC (BioLegend, clone UCHT1), 1:100-diluted anti-human CD4-PE (BioLegend ...
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bioRxiv - Bioengineering 2023Quote: ... and stained with the following antibodies in FACS staining buffer (PBS with 2% Fetal Bovine Serum, 1% Penicillin-Streptomycin): anti-human CD31 (BioLegend, WM59, FITC), anti-human CD45 (BioLegend ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... 5 and 10 µM) for 5 days were collected and stained with FITC-CD41 and PE-CD42b (BioLegend, San Diego, CA, USA) antibodies for 15 min on ice in the dark ...
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bioRxiv - Bioengineering 2024Quote: The following anti-human antibodies were used in the PBMC staining panel for purity: anti-CD4 FITC (BioLegend catalog #317408, clone OKT4), anti-CD20 PerCP/Cy5.5 (BioLegend catalog #375510 ...
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bioRxiv - Immunology 2024Quote: Cells were incubated for 10 mins with Fc block (homemade containing 24G2 supernatant and mouse serum) surface stained with anti-CD45 FITC (Biolegend, 30-F11) or anti-CD45-PE (BD ...
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bioRxiv - Cancer Biology 2024Quote: ... the pellets were incubated for 1 hour in 60 µL of staining solution (1% BSA and 0.5% Tween-20 in PBS) containing 3 µL of anti-BrdU-FITC monoclonal antibody (Biolegend 364104, clone 3D4). The samples were subsequently washed with 1 mL of 1% BSA and 0.5% Tween-20 in PBS ...
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bioRxiv - Immunology 2021Quote: ... The depleted cell fraction was then stained for 20 min at 4 °C with a cocktail of fluorophore-labeled antibodies CD45-PerCPCy5.5 (1:50) (Biolegend), Ly51-PE (1:800 ...
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bioRxiv - Immunology 2021Quote: ... and stained with fluorochrome-labeled anti-mouse IFN-γ/TNF-α/IL-2/IL-4/IL-5 mAbs (Biolegend). LSRFortessa was used to acquire the flow cytometry data ...
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bioRxiv - Immunology 2021Quote: The following antibody mixture was made and used to stain the flow through and labeled fractions: B220-BV510 (Biolegend) at 1:20 ...
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bioRxiv - Cancer Biology 2020Quote: ... we collected the beads and labeled them with 4 µg/ml PE anti-CD63 antibody (BioLegend, clone NVG-2) for 45 minutes at 37°C ...
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bioRxiv - Immunology 2022Quote: ... cells were incubated with A32-APC (3.3 μg/ml) for 25 minutes and labeled with anti-CD3-AF700 (SK7, Biolegend) and anti-CD32-PE-Cy7 (FUN-2 ...
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bioRxiv - Bioengineering 2022Quote: ... and then stained with the fluorescently labeled antibody that detects the corresponding cell surface receptor (anti-EGFR-BV421, Biolegend, Cat ...
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bioRxiv - Immunology 2021Quote: ... counted and up to 1×108 cells were labeled with 1.5-2 μM Carboxyfluorescein succinimidyl ester (CFSE; BioLegend, USA) in 1 mL PBS 1X for 20 min according to the manufacturer’s protocol ...
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bioRxiv - Immunology 2020Quote: ... OVA presentation was confirmed by flow cytometry using a PE-labeled antibody against surface SIINFEKL bound to H-2Kb (clone 25-D1.16, BioLegend).
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bioRxiv - Systems Biology 2021Quote: ... and then stained the cells for 30 min with 1:500 anti-NGFR APC-labeled clone ME20.4 (BioLegend, 345107). We washed the cells five times with 0.1% BSA/PBS and followed with one final wash with PBS for 2 min at room temperature ...
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bioRxiv - Cell Biology 2020Quote: ... Stem and progenitor cells were isolated using fluorescently labeled or biotinylated antibodies for the following antigens: cKit (2B8, Biolegend), Sca1 (D7 ...
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bioRxiv - Immunology 2024Quote: ... Then the cells were labeled as described above using antibodies anti-CD3-BV421 (clone 17A2) (BioLegend, SanDiego, CA, USA) (1:200 ...
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bioRxiv - Immunology 2022Quote: ... MR1 surface expression was evaluated by flow cytometry with APC-labeled mouse anti-MR1 mAbs 26.5 (Biolegend, Cat#361108) and APC-labeled mouse IgG2a (clone MOPC-173 Biolegend ...
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bioRxiv - Immunology 2022Quote: ... and stained with a cocktail of fluorescently labeled anti-cytokine antibodies: IFN-γ PE/Dazzle 594 (Biolegend, cat.505845), TNF-α PE/Cyanine7 (Biolegend ...
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bioRxiv - Cancer Biology 2022Quote: ... Single cell suspensions from individual tumors were then labeled with hashtag oligonucleotides following the manufacturer’s protocol (TotalSeq B0301-B0306, Biolegend). Each individual tumor sample was counted and then pooled at an equal cell ratio before being split into two replicates for library preparation with the Chromium Single Cell 3’ V3 (10X Genomics ...
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bioRxiv - Neuroscience 2023Quote: ... For further experiments viable CD45highCD8+ cells were labeled with rat anti-CD45 APC (1:100, catalog no. 103111; BioLegend), rat anti-CD8 PerCP/Cyanine5.5 (1:100 ...
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bioRxiv - Immunology 2022Quote: ... All enriched samples were stained with the following panel of TotalSeq-A-labeled anti-mouse antibodies to detect protein expression in the scRNA-seq dataset: Ly6C (HK1.4, BioLegend), CD44 (IM7 ...
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bioRxiv - Cell Biology 2024Quote: ... cells were labeled with antibodies detecting the following cell-surface markers: CD133-APC (Biolegend, clone 7, dilution 1:20), CD19-PECy7 (Biolegend ...
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bioRxiv - Immunology 2024Quote: ... Enriched samples were then stained on ice for 30 minutes with the following antibodies: BV421-labeled CD45.1 (A20, BioLegend), BV785-labeled CD45.2 (104 ...
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bioRxiv - Immunology 2024Quote: ... to lyse the red blood cells and then stained on ice for 30 minutes with the following antibodies: BV421-labeled CD45.1 (A20, BioLegend), BV785-labeled CD45.2 (104 ...
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bioRxiv - Immunology 2024Quote: ... as well as stained with activation marks Brilliant Violet 421-labeled anti-human CD38 mAb (Clone HIT2, BioLegend, USA), APC-labeled anti-human HLA-DR mAb (Clone L243 ...
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bioRxiv - Bioengineering 2024Quote: ... Cells from each sample were labeled with TotalSeq™-B0251-0254 anti-human Hashtag Antibodies (clone LNH-94, Biolegend, RRID ...