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Citations for BioLegend :
1951 - 2000 of 8287 citations for DNAM 1 Human HEK 293 Fc since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2023Quote: ... TCR-transduced CD8+ T cells were rapidly expanded for 5 days using irradiated feeder PBMCs and medium containing 60U/ml IL-2 (Proleukin, Novartis) and Ultra-LEAF™ Purified anti-human CD3 Antibody (clone OKT3, BioLegend 317326, 1:2000). After 5 days ...
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bioRxiv - Immunology 2023Quote: ... pDCs were gated for flow cytometry with 1:20 dilution commercial mouse anti-human BDCA-2-Alexa488 (stock 200 ug/mL, BioLegend, San Diego, CA, USA) and 1:20 dilution mouse anti-human CD123 (IL-3)-PE (stock 200 ug/mL ...
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bioRxiv - Neuroscience 2024Quote: ... flow cytometry staining master mix was prepared containing 1:200 dilutions of PE anti-mouse/human CD11b Antibody (BioLegend, Cat#:101208, clone M1/70), APC anti-mouse CX3CR1 (Antibody BioLegend ...
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bioRxiv - Bioengineering 2024Quote: ... The sections were subsequently stained with a rat anti-human YAP antibody conjugated with PE (dilution: 1:500; clone W19260A; Biolegend, San Diego, CA, USA) for 2 hours at room temperature ...
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bioRxiv - Cancer Biology 2023Quote: ... peripheral blood and spleens were harvested from each mouse and the infiltrated primary cells were identified using flow cytometry by staining for human CD45 antibody (BioLegend; BLG-368522; 1:200).
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bioRxiv - Cell Biology 2020Quote: ... Brilliant Violet 421 anti-human CD45 and CD31 combined with a panel of cell-surface proteins targeting 332 PE-conjugated monoclonal antibodies (Biolegend, LEGENDScreen Human PE Kit, 700007) and 10 isotype controls (Biolegend ...
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bioRxiv - Immunology 2021Quote: Mouse B cells were bulk sorted from mixed chimeras into Eppendorf tubes containing cold sterile PBS 10% FCS and incubated for 20 min at 4°C with TotalSeq™ DNA-barcoded anti-mouse ‘Hashing’ antibodies (BioLegend) at a 1/100 final dilution ...
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bioRxiv - Immunology 2020Quote: ... CD19-CAR moiety was labeled by AF647-conjugated anti-mouse F(ab’)2 antibody or biotin-conjugated rhCD19-Fc (ACRO Biosystems, USA) with AF647-conjugated streptavidin (Biolegend, USA). ΔLNGFR was labeled with APC-conjugated LNGFR antibody (clone ME20.4-1.H4 ...
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bioRxiv - Immunology 2019Quote: The percentage of CAR and ΔLNGFR positive T cells was assessed by biotin-conjugated rhCD19-Fc (Cat # CD9-H5259, ACRO Biosystems, USA) with AF647-conjugated streptavidin (Cat # 405237, Biolegend, USA), and fluorescein isothiocyanate (FITC)-conjugated LNGFR antibody (Cat# 130-112-605 ...
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bioRxiv - Immunology 2020Quote: Fc block was performed using TruStain FcX™ CD16/32 antibody (anti-mouse) to avoid non-specific binding (101320 Biolegend, UK) according to the supplier protocols The fluorochrome-conjugated antibodies used in the panel were obtained from Biolegend ...
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bioRxiv - Immunology 2022Quote: The following protocol was used for all tumor experiments: after the Fc receptor of the cells was blocked with anti-mouse CD16/32 (Biolegend, 101320), extracellular staining was performed in FACS buffer for 30 minutes ...
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bioRxiv - Immunology 2022Quote: ... samples were pre-incubated for 5 minutes at 4°C with TruStain FcX™ (anti-mouse CD16/32) Fc block (clone 93; BioLegend). Samples were then stained for extracellular markers in the presence of Fc block for 30 minutes at 4°C protected from light using the following antibodies ...
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bioRxiv - Immunology 2023Quote: ... Cells were pelleted at 500xg for 5 minutes and then resuspended in 50uL of PBS+ 2% HI-FBS + 2mM EDTA in the presence of Fc receptor blocking antibody (BioLegend, 101302). Cells were labelled with antibodies as described below ...
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bioRxiv - Immunology 2023Quote: ... Cells were stained with Zombie-violet or Zombie-NIR in PBS at 1:2000 dilution for 5 minutes at room temperature prior to resuspending in PBS + 2% HI-FBS + 2mM EDTA in the presence of Fc receptor blocking antibody (BioLegend, 101302) for blocking ...
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bioRxiv - Microbiology 2023Quote: ... Each well was blocked using 50 μL of FACS buffer (PBS + 5% FBS) + 2.5 μL Fc receptor (BioLegend, Cat No. 422302) block per well and then 150 μL FACS buffer was added and spun down ...
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bioRxiv - Immunology 2023Quote: ... and stained with a master mix of antibodies, viability dye (Ebioscience, Cat# 65-0865-14) and Fc block (Biolegend, Cat#422302) in FACS buffer for surface staining ...
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bioRxiv - Physiology 2023Quote: ... Cisplatin is washed off and non-specific binding was blocked with a 10-minute staining of cells with Fc Block (Biolegend; 1µg). Cells were stained with 0.5µg of cell surface metal-conjugated antibodies for 30 minutes at room temperature ...
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bioRxiv - Immunology 2023Quote: ... Antibody staining was performed at a dilution of 1:100 for 25 minutes at 4L°C in the presence of mouse Fc block (TruStain FcX™, anti-mouse CD16/32, BioLegend) in PBS containing 5% FBS ...
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bioRxiv - Neuroscience 2020Quote: ... anti-neurofilament to a phosphorylated heavy chain neurofilament (NF-H; mouse anti-human monoclonal antibody SMI-34, dilution 1:100, epitope retrieval time 10 min, Biolegend 835503; Biolegend, San Diego, CA). Hemosiderin deposition was evaluated on a section with only counterstain ...
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bioRxiv - Immunology 2022Quote: The following human cytokines were assessed by ELISA as previously described (10): human IFN-γ (capture MD-1 mAb; revealing biotinylated 4S.B3 mAb, Biolegend Cat#507502 and 502504, respectively), human IL-13 (capture JES10-5A2 mAb ...
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bioRxiv - Cell Biology 2022Quote: ... and 0.1% Tween-20 and incubated overnight at 4°C in a humidified chamber with primary human anti-CD9 (Biolegend, catalog no. 312102, dilution 1:1000), human anti-CD63 (BD ...
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bioRxiv - Immunology 2023Quote: ... and then stained for 20 minutes at 4 °C using the following antibodies: Brilliant Violet 605™ anti-human interferon-gamma (IFN-γ) (506542, Biolegend; 1 μL/well). After being stained ...
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bioRxiv - Cancer Biology 2023Quote: ... The bone marrow was harvested from each mouse and the infiltrated CCRF cells were identified by staining for a human CD45 antibody (Biolegend; AB_2687375; BLG-368522; 1:200).
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bioRxiv - Immunology 2021Quote: Recombinant human ARG1 was obtained from Biolegend (San Diego, CA, USA), recombinant murine Arg1 was obtained from Cloud-Clone Corp. ...
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bioRxiv - Cancer Biology 2022Quote: ... Alexa Fluor® 647 anti-human CD326 (EpCAM) Antibody (Biolegend 324212), PE anti-human/mouse CD49f Antibody (Biolegend 313612) ...
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bioRxiv - Immunology 2021Quote: ... biotin anti-human CD19 (BioLegend, San Diego, CA, USA, Cat. 302203) was used as a primary stain ...
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bioRxiv - Immunology 2019Quote: ... sections were labeled with mouse anti-human CD45-APC (HI30, Biolegend) for 1hr at room temperature ...
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bioRxiv - Immunology 2019Quote: ... sections were labeled with mouse anti-human CD34-APC (561, Biolegend) for 1hr at room temperature ...
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bioRxiv - Immunology 2020Quote: ... Brilliant Violet 605™ anti-human CD56 (NCAM) Antibody (HCD56, Biolegend). The following antibodies were used for intracellular staining ...
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bioRxiv - Immunology 2020Quote: ... Brilliant Violet 605™ anti-human CD56 (NCAM) Antibody (HCD56, Biolegend). CAR transduction was measured using anti-CAR antibody Goat F(ab’)2 anti-Human IgG (H+L)-Alexa Fluor 647 (Jackson Laboratories) ...
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bioRxiv - Immunology 2019Quote: ... with an anti-human HLA-E Fab (generated from 3D12, BioLegend), or with isotype-matched control mouse IgG1 Fab (generated from MG1-45 ...
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bioRxiv - Bioengineering 2019Quote: ... Mouse anti-Human IgG was purchased from Biolegend (San Diego, CA). Bovine Serum Albumin and Goat Anti-Mouse IgG HRP conjugate were purchased from Millipore Sigma (St ...
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bioRxiv - Developmental Biology 2021Quote: ... 2.5 µl of PE/Cy7 anti-human CD71 antibody (BioLegend, 334111) was added and incubated at 4 degree for 30 min ...
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bioRxiv - Immunology 2021Quote: ... and resuspended with antibody staining buffer containing human TruStain FcX (Biolegend). Cells were incubated with TotalSeq-C ...
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bioRxiv - Immunology 2021Quote: ... then labeled with unique anti-human TotalSeq-B hashing antibodies (BioLegend). Following labeling ...
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bioRxiv - Bioengineering 2021Quote: ... and APC anti-human CD46 (clone TRA-2-10, Biolegend 352405). Antibody staining was performed for 20 minutes on ice ...
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bioRxiv - Genomics 2021Quote: After the addition of 5 ul Human TruStain FcX™ (BioLegend), the cell suspension was incubated for 10 min on ice ...
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bioRxiv - Cell Biology 2021Quote: ... and APC-conjugated anti-human PMN Ly6G/6C antibodies (Biolegend # 108412) in PBS containing 1% BSA and 0.05% sodium azide ...
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bioRxiv - Cancer Biology 2021Quote: ... and Brilliant Violet 650™ anti-human CD69 (BioLegend, cat. 310934). Harvested PBMC was stained with the prepared master mix for 20 minutes at 4°C and fixed using 1% v/v paraformaldehyde in DPBS for 15 minutes at room temperature ...
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bioRxiv - Immunology 2021Quote: ... Cells were stained with PE anti-human HLA-A2 antibody (BioLegend) at 4 °C in the dark for 30 min ...
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bioRxiv - Immunology 2021Quote: ... APC/Fire 750 anti-human KLRG1 (MAFA) antibody (clone SA231A2, BioLegend), BV421 anti-human CD335 (NKp46 ...
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bioRxiv - Cancer Biology 2020Quote: ... anti-mouse/human Granzyme B AF647 (515405) (BioLegend, San Diego, CA). Flow cytometry was performed on a LSRII flow cytometer or FACSCalibur (BD Biosciences) ...
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bioRxiv - Cancer Biology 2022Quote: ... Cells were first incubated with Human FcX blocking solution (Biolegend, #422302) and then incubated with the surface antibody CD34-PE-Vio770 (clone AC136 ...
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bioRxiv - Bioengineering 2022Quote: ... and stained with anti-human CD45 APC antibody (clone HI30, BioLegend) to evaluate human CD45+ engraftment by FACS ...
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bioRxiv - Immunology 2022Quote: ... α-human HLA-A,B,C (W6/32, conjugated to APC, Biolegend). Phytohemagglutinin PHA-L (L4144 Sigma ...
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bioRxiv - Immunology 2022Quote: ... and PE-anti-human CD56/NCAM (HCD56; BioLegend, San Diego, CA) for T cells and NK cells ...
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bioRxiv - Immunology 2022Quote: ... APC-anti human 2B4/CD244 (2-69; BioLegend, San Diego, CA); or with the isotype control APC mouse IgG1 (MOPC-21 ...
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bioRxiv - Immunology 2022Quote: ... 3.5 μL Brilliant Violet 421 anti-human CD27 antibody (BioLegend, 302824), 3 μL PE/Cyanine7 anti-human IgM antibody (BioLegend ...
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bioRxiv - Immunology 2022Quote: ... 3.5 μL Brilliant Violet 421 anti-human CD27 antibody (BioLegend, 302824), 2 μL PE/Cyanine7 anti-human IgM antibody (BioLegend ...
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bioRxiv - Bioengineering 2022Quote: ... and Brilliant Violent 785 anti-human CD14 (Clone 63D3, Biolegend 367142) in Cell Staining Buffer (Biolegend ...