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Citations for BioLegend :
1801 - 1850 of 8287 citations for DNAM 1 Human HEK 293 Fc since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2022Quote: The collected BMDCs were centrifuged once and resuspended in cell staining buffer (PBS supplemented with 2% FBS) containing anti-Fc receptor mAb (Biolegend). Cells were blocked for 10 min at 4°C ...
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bioRxiv - Immunology 2021Quote: ... Resulting single cell suspensions of splenocytes or bone marrow cells were Fc blocked with Trustain FcX (anti-mouse CD16/32 clone 93-Biolegend) and stained with combinations of the following antibodies ...
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bioRxiv - Immunology 2021Quote: ... Single cell suspensions were blocked with rat anti-mouse FcγIII/II receptor (CD16/CD32) blocking antibodies (“Fc-Block”) and stained with live/dead cell-exclusion dye (Zombie UV dye; Biolegend). The cells were then incubated with fluorophore-conjugated antibodies directed against cell surface antigens ...
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bioRxiv - Immunology 2022Quote: Slices were placed on a microscopy slide and Fc receptors on cells were blocked to avoid nonspecific staining using 20 μl purified anti-mouse CD16/32 antibody (BioLegend) in 1X PBS with 2% FBS at a concentration of 20μg/ml for 30 min at 37 °C ...
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bioRxiv - Immunology 2023Quote: ... Enriched cells were washed once in wash buffer and an Fc blocking step was performed using TruStain FcX (anti-mouse CD16/32) antibody (BioLegend) according to the manufacturer’s instruction.
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bioRxiv - Immunology 2023Quote: ... Cells were incubated with anti-CD16/CD32 (clone 93, eBioscience, 14-0161-86) for Fc receptor blockade and stained with Zombie Yellow (Biolegend) for 10 minutes at room temperature in the dark to distinguish live and dead cells ...
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bioRxiv - Cell Biology 2023Quote: Flow cytometry of circulating leukocytes was performed by first blocking Fc receptors by incubating cells with TruStain FcX PLUS (anti-mouse CD16/32, Biolegend) for 10 minutes ...
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bioRxiv - Immunology 2023Quote: ... Cells were centrifuged at 400 g for 8 minutes and stained with lanthanide-labeled antibodies against immunophenotypic markers in CyTOF buffer containing Fc receptor blocker (BioLegend) for 30 minutes at room temperature (RT) ...
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bioRxiv - Immunology 2023Quote: ... single cell suspensions were incubated on ice for 10 minutes with Fc receptor-blocking anti-CD16/32 (BioLegend Cat# 101301). Cell suspensions were first stained for 5 minutes at room temperature with LIVE/DEAD® Fixable Red Dead Cell Stain Kit (ThermoFisher Cat# L23102) ...
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bioRxiv - Cancer Biology 2023Quote: ... for dead cell exclusion followed by Fc-receptor blocking with purified mouse CD16/32 antibody (BioLegend, catalog #101301, clone 93). After cell surface marker staining with fluorescent-conjugated antibodies ...
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bioRxiv - Immunology 2023Quote: ... splenocytes were resuspended in 200 uL PBS containing 2% FBS and Fc block and incubated with biotin anti-mouse F4/80 (Clone BM8, BioLegend), CD3 (Clone 500A2 ...
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bioRxiv - Immunology 2024Quote: ... FcγII/III receptors were blocked by prior 10 min incubation with Fc block (anti mouse CD16/32 antibody Clone 93, Biolegend) on ice.
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bioRxiv - Immunology 2020Quote: ... pooled from a minimum of four healthy human donors) and 50 ng/mL of recombinant human MΦ colony-stimulating factor (M-CSF) (Biolegend; San Diego, CA, USA). Monocytes were cultured in monocyte culture media at a cellular density of 1.5 x 106 cells/mL in 6-well cell culture plates ...
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bioRxiv - Immunology 2023Quote: Plasma and culture supernatants were collected and frozen at -80°C until cytokines measurement by commercial ELISA kits (human IL-1ß and IL-18 R&D Systems; human TNF-α and IFN-γ, Biolegend).
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bioRxiv - Cancer Biology 2021Quote: ... The PE anti-human CD274/PD-L1 (Biolegend, catalog# 329706) antibody and the isotype control antibody (PE Mouse IgG2b ...
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bioRxiv - Cancer Biology 2021Quote: ... APC anti-human CD3 antibody (clone HIT3a, catalog #300319, BioLegend). The 10x library was sequenced on an Illumina HiSeq 2500 instrument.
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bioRxiv - Cell Biology 2020Quote: ... APC-Fire 750 anti-human CD20 (302357, clone 2H7, BioLegend), and APC-H7 anti-human CD3 (560275 ...
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bioRxiv - Immunology 2021Quote: ... and APC-conjugated mouse anti-human IgD (Biolegend, CA, USA). To determine the purity of CB-SC ...
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bioRxiv - Immunology 2020Quote: ... and 50 ng/ml recombinant human M-CSF (BioLegend #574806) for 6 days to obtain monocytes derived macrophages ...
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bioRxiv - Immunology 2021Quote: ... 5ng/ml or 50ng/ml recombinant human G-CSF (BioLegend) or mouse G-CSF (BioLegend) ...
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bioRxiv - Bioengineering 2019Quote: Devices stained with Alexa Fluor 647 anti-human CD31 (BioLegend) and Alexa Fluor 594 Phalloidin (ThermoFisher ...
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bioRxiv - Cancer Biology 2019Quote: ... or PerCP and human anti-CD19-PE were from BioLegend, BD Biosciences ...
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bioRxiv - Immunology 2019Quote: ... anti-mouse/human CD11b APC-Cy7 (clone M1/70, Biolegend), anti-mouse CD11c PE-eFluor 610 (clone N418 ...
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bioRxiv - Cancer Biology 2019Quote: ... Anti-human CD45 (clone: 2D1, # 368516) was obtained from BioLegend, San Diego ...
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bioRxiv - Immunology 2021Quote: ... Then the samples were treated with Human TruStain FcX (Biolegend) to block Fc receptors ...
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bioRxiv - Neuroscience 2020Quote: ... anti-mouse/human CD11b APC-Cy7 (clone M1/70, Biolegend), anti-mouse CD11c PE-eFluor 610 (clone N418 ...
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bioRxiv - Cancer Biology 2021Quote: human HLA-A,B,C-PE (W6/32, Biolegend 311406), FC ...
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bioRxiv - Bioengineering 2021Quote: ... and PE/Cyanine 7 anti-human CD4 antibody (#317414, Biolegend), APC/Cyanine 7 anti-human CD8 antibody (#344714 ...
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bioRxiv - Bioengineering 2020Quote: ... and LEGENDplex™ Human Inflammation Panel were acquired from BioLegend.
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bioRxiv - Bioengineering 2020Quote: Human IFNγ was quantified via sandwich ELISA assay (430107, Biolegend) and per the manufacturer’s recommended conditions.
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bioRxiv - Immunology 2020Quote: ... Cells were stained with custom TotalSeq-C human antibodies (BioLegend) before loading onto a Chromium Next GEM chip G ...
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bioRxiv - Immunology 2021Quote: ... PE anti-mouse/human GL7 antibody (BioLegend, catalog no. 144608), Brilliant Violet 605 anti-mouse CD95 (Fas ...
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bioRxiv - Microbiology 2021Quote: ... Cytokines were measured using human ELISA Max deluxe kits (BioLegend) according to the manufacturer’s instruction.
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bioRxiv - Cancer Biology 2021Quote: ... Brilliant Violet 785™ anti-human CD4 (BioLegend, cat. 317442) and Brilliant Violet 650™ anti-human CD69 (BioLegend ...
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bioRxiv - Immunology 2020Quote: ... and the following anti-human antibodies: CD3-BV605 (UCHT1, Biolegend) or CD3-Super Bright 600 (UCHT1 ...
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bioRxiv - Cancer Biology 2021Quote: Alexa Fluor 488 anti-human CD326 (Ep-CAM) antibody (BioLegend); cat # 324210
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bioRxiv - Cell Biology 2021Quote: ... and incubated with anti-mouse/human CD207 PE (Biolegend, 144204) and either anti-mouse MHCII APC (Biolegend ...
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bioRxiv - Cell Biology 2020Quote: ... Percp-Cy5.5-anti-mouse/human CD11b (clone M1/70, Biolegend), PE-Cy7-anti-mouse F4/80 (clone BM8 ...
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bioRxiv - Immunology 2022Quote: ... 2 μL PE/Cyanine7 anti-human IgD antibody(BioLegend, 348210), 0.0032 μg biotinylated SARS-CoV-2 BA.1 protein (His & AVI Tag ...
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bioRxiv - Microbiology 2022Quote: ... and ELISA MAX Deluxe Set Human IL-1b (Biolegend, 437004), per the manufacturer’s instructions ...
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bioRxiv - Immunology 2022Quote: ... Brilliant Violet 421™ anti-human CD27 antibody (BioLegend, 302824), PE/Cyanine7 anti-human IgM antibody (BioLegend ...
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bioRxiv - Immunology 2022Quote: ... and mouse anti-human MAC1 (α-αMβ2, CBRM1/5, Biolegend) were incubated with 30 fold molar excess of sulfo-SMCC cross-linker (ThermoFisher ...
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bioRxiv - Immunology 2022Quote: ... stained with phycoerythrin (PE)-conjugated mouse anti-human CD19 (BioLegend, clone HIB19 ...
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bioRxiv - Immunology 2022Quote: ... 3 μL PE/Cyanine7 anti-human IgM antibody (BioLegend, 314532), 0.0052 μg biotinylated Ovalbumin (SinoBiological ...
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bioRxiv - Immunology 2022Quote: ... MojoSort human CD14+ monocytes isolation kit was purchased from Biolegend. Cell culture media DMEM and RPMI-1640 were purchased from Hyclone.
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bioRxiv - Molecular Biology 2022Quote: ... and The LEGEND MAX Human IL-1β ELISA Kit (Biolegend) was used to assay for IL-1β concentration ...
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bioRxiv - Microbiology 2022Quote: ... and FITC anti-human/mouse Granzyme B recombinant antibody (Biolegend). Samples were analyzed on the CytoFLEX LX flow cytometer (Beckman Coulter).
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bioRxiv - Cell Biology 2022Quote: ... Cells were then incubated with anti-human CD15 PE (Biolegend) for 1 hour prior to FACS analysis ...
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bioRxiv - Immunology 2022Quote: ... 2 μL PE/Cyanine7 anti-human IgM antibody (BioLegend, 314532), 2 μL PE/Cyanine7 anti-human IgD antibody(BioLegend ...
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bioRxiv - Bioengineering 2022Quote: ... or Alexa Fluor™ 594-conjugated anti-human Prox1 (Biolegend) to image cell nuclei at a 1:100 and 1:1000 dilution from stock in washing buffer (0.5% BSA/DPBS) ...