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Citations for BioLegend :
1751 - 1800 of 8287 citations for DNAM 1 Human HEK 293 Fc since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2023Quote: Mouse anti-human CD56-PC5 (Clone: 5.1H11; Biolegend, 362516), Verified Reactivity ...
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bioRxiv - Cell Biology 2023Quote: Mouse anti-human CD15-APCfire750 (Clone: W6D3; Biolegend, 323041), Verified Reactivity ...
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bioRxiv - Cell Biology 2023Quote: Mouse anti-human CD34-BV421 (Clone: 561; Biolegend, 343610), Verified Reactivity ...
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bioRxiv - Genomics 2023Quote: CD97 (ADGRE5): FITC anti-human CD97 antibody (Biolegend, 336306)
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bioRxiv - Genomics 2023Quote: CD26 (DPP4): FITC anti-human CD26 antibody (Biolegend, 302704)
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bioRxiv - Immunology 2023Quote: ... anti-human CXCR5-Alexa Fluor 647 (clone J252D4, BioLegend), anti-human IgA-FITC (clone IS11-8E10 ...
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bioRxiv - Cell Biology 2023Quote: ... and PE-conjugated anti-human DR5 antibody (B347680, Biolegend), both at 1 µg/ml ...
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bioRxiv - Cell Biology 2024Quote: ... Alexa Fluor 488 anti-human CD326 (Epcam; Biolegend, 324210), APC/Cy7 anti- human CD45 (Biolegend ...
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bioRxiv - Immunology 2024Quote: ... or PE anti-human CD25 (clone: BC96; BioLegend®) antibodies ...
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bioRxiv - Immunology 2024Quote: ... Brilliant Violet 421 anti-human CD4 Antibody (#357424, BioLegend), PE anti-human TCR Vα24-Jα18 (iNKT cell ...
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bioRxiv - Cancer Biology 2024Quote: ... 20 ng/mL recombinant human thrombopoietin (hTPO) (BioLegend #597402), DNase (100 μg/mL final concentration) ...
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bioRxiv - Immunology 2021Quote: ... Cells were incubated at 4°C for 15 minutes with an Fc receptor blocker (BioLegend TruStain FcX, cat # 422302) according to manufacturer instructions before cocktails of surface antibodies were added for 30 minutes at 4°C ...
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bioRxiv - Cancer Biology 2021Quote: ... Red blood cells were lysed (15mM NH4Cl + 10mM KHCO3 for 5 min at R.T.) and CD16/32 antibody was used to block the endogenous FC (#101301, Biolegend). Cells were analysed using a BD FACS LSR Fortessa ...
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bioRxiv - Immunology 2021Quote: ... the cells were incubated for 5 min on ice with Fc Block (TruStain fcX anti-mouse CD16/32, Biolegend) and washed with 200 μL FACS buffer ...
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bioRxiv - Immunology 2021Quote: ... Siglec-Fc were used at approximately 20 μg/mL and AF647 Goat anti-hIgG (Clone: HP6017, 0.4 μg/mL, Biolegend) were mixed together in a 1:1 volume ratio right before staining ...
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bioRxiv - Immunology 2022Quote: ... mouse TNF-α (clone MP6-XT22), mouse CD8α (clone 53-6.7), and mouse CD16/32 (Fc block, clone 93) were purchased from Biolegend. Other reagents used include Zombie Aqua Viability Dye (Biolegend) ...
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bioRxiv - Immunology 2020Quote: Non-specific binding of antibodies to Fc-receptors was blocked with anti-mouse CD16/CD32 (TruStain fcX from BioLegend) in single cell suspensions prepared from Spl ...
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bioRxiv - Molecular Biology 2021Quote: ... Fc receptors were blocked by incubating cells with TruStain FcX™ PLUS anti-mouse CD16/32 antibody (Biolegend, USA) for 10 min ...
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bioRxiv - Bioengineering 2020Quote: ... and treated with Fc-receptor blocker (2.5 μ g/mL, anti-mouse CD16/32; BioLegend, Cat# 101301, RRID: AB_312800) for 20 minutes at 4 °C in the dark ...
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bioRxiv - Immunology 2020Quote: ... Blocking of Fc receptors in T2 cells was performed prior to staining using the TruStain FcX reagent (BioLegend, #422301). Staining with peptide-MHC dextramers was performed for 10 min at room temperature (RT) ...
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bioRxiv - Immunology 2022Quote: ... Samples were washed in PBS and were incubated at 4°C with CD16/32 Fc block (cat#101302, BioLegend) in PBS for 10 minutes before staining with antibodies (Supplementary Table 1 ...
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bioRxiv - Immunology 2022Quote: ... before leukocytes were separated using 30 and 70% Percoll gradients and subsequently stained for 30 minutes in FACS buffer (PBS/2.5%FCS/10mM EDTA/0.01% NaN3) with the following primary antibodies: CD45-PacificBlue (Biolegend, 103126), CD19-biotin (Biolegend ...
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bioRxiv - Cell Biology 2023Quote: ... approximately 200,000 cells per sample were resuspended in staining buffer (PBS, 2% BSA, and 0.01% Tween) and incubated for 10 minutes with Fc block (TruStain FcX (BioLegend) and FcR blocking reagent (Miltenyi) ...
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bioRxiv - Immunology 2023Quote: ... Cells were washed with LunaStain cell staining buffer (Polaris Biology, China) and first stained with Fc block (Biolegend, USA) for 10 min at room temperature ...
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bioRxiv - Immunology 2023Quote: ... Cells were incubated 20 min on ice with anti-CD16/32 (Fc-block, clone 93, BioLegend, San Diego, CA) then stained with the following antibodies directed toward cell surface receptors ...
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bioRxiv - Immunology 2023Quote: ... Cells were subsequently resuspended in staining buffer (PBS with 0.5% bovine serum albumin and 2 mM EDTA) and Fc-blocked using TruStain FcX reagent (BioLegend). Cells were then stained with the following fluorophore-conjugated anti-mouse antibodies for 30 min at 4°C ...
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bioRxiv - Developmental Biology 2023Quote: ... then resuspended in ice-cold MACS buffer (DPBS + 0.5% BSA + 2mM EDTA) and incubated with Fc Block (Biolegend 156603) for 5 min at 4°C ...
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bioRxiv - Cell Biology 2022Quote: ... one million cells were first resuspended in 100 μL buffer containing 5 μL Fc Receptor Blocking Solution (Biolegend, #422302) and incubated for 15 minutes ...
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bioRxiv - Immunology 2023Quote: ... and single cell preparations were obtained by passing the cell mixture through a PluriStrainer (500 – 70µM cell strainers; 43-50500, PluriSelect) before blocking with Fc block for 10 min (422302, BioLegend). CD45+ cells were enriched using anti-CD45 magnetic MicroBeads and LS columns (130-045-801 and 130-042-401 ...
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bioRxiv - Neuroscience 2024Quote: ... Cells were then blocked on ice with PBS + 7.5% FCS for 15 minutes before staining with antibody cocktails (see key resource table) containing TruStain Monocyte Blocker (Biolegend) in FACS buffer for 45 minutes at 4°C ...
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bioRxiv - Neuroscience 2020Quote: ... Aβ peptides were extracted from 250 µL human plasma using immunoprecipitation with anti-β-Amyloid 17-24 (4G8) and anti-β-Amyloid 1-16 antibodies (6E10, both Biolegend®) coupled to Dynabeads™ M-280 Sheep Anti-Mouse IgG magnetic beads (Thermofisher ...
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bioRxiv - Cancer Biology 2021Quote: ... freshly harvested PDX or EHS tumors were processed to single cells47 and stained with PE-conjugated anti-human CD298 PE (BioLegend; 1:50 dilution) and FITC-conjugated anti-mouse CD45 FITC (BioLegend ...
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bioRxiv - Neuroscience 2020Quote: ... anti-neurofilament to a phosphorylated heavy chain neurofilament (NF-H; mouse anti-human monoclonal antibody SMI-34, dilution 1:100, epitope retrieval time 10 min, Biolegend 835503; Biolegend, San Diego, CA). Hemosiderin deposition was evaluated on a section with only counterstain ...
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bioRxiv - Neuroscience 2022Quote: ... human Aβ was immunodepleted from TgCM with the mouse antibody 6E10 (Purified anti-β-Amyloid, 1-16 antibody BioLegend Cat# 803004, RRID: AB_2715854) and Protein G Sepharose beads (Sigma-Aldrich) ...
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bioRxiv - Microbiology 2023Quote: ... was added to measure and gate on the % live HL-60 cells and anti-mouse/human CD11b PE (Biolegend, Catalog #101207, 1:50) was added to measure and gate on differentiated HL-60 cells ...
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bioRxiv - Bioengineering 2023Quote: ... coated glass bottom chamber and then labeled with 1ugꞏml−1 of Alexa Flour (AF) 488 conjugated anti-human CD45 antibodies (Biolegend, Cat# 304017, clone HI30) in 2% BSA in PBS for 20 min at RT ...
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bioRxiv - Microbiology 2024Quote: ... The panel of cytokines presented in Fig 5 was measured using the LEGENDplex™ Human Inflammation Panel 1 (13-plex) (BioLegend, Cat# 740809) kit ...
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bioRxiv - Bioengineering 2024Quote: ... Sections were then stained overnight at 4 °C in a humidified chamber with mouse monoclonal anti-human CD68 (hCD68, 1:400, BioLegend, clone Y1/82A) and rabbit polyclonal anti-mouse IL-10 (mIL-10 ...
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bioRxiv - Cancer Biology 2019Quote: ... supernatants were harvested and stored at −80°C until the amount of liberated cytokine could be quantified by ELISA MAXTM Human IL-2 or Human IFN-g detection kits (BioLegend #431801 and #430101).
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bioRxiv - Cancer Biology 2020Quote: ... Alexa Fluor 488 anti-human CD206 and PE/Cy7 anti-human Interleukin-10 (IL-10) were used for analysis (all from BioLegend, San Diego, CA). The samples were run on an LSR Fortessa cell analyzer from BD (Becton Dickinson ...
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bioRxiv - Immunology 2019Quote: ... mouse anti-human CD247 (CD3ζ) mAb (clone 6B10.2) and mouse anti-human EGFR antibody (clone AY13) were purchased from Biolegend (San Diego, CA, USA). Anti-human CD32 mAb (clone 8.26 ...
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bioRxiv - Immunology 2021Quote: ... 1 million cells pooled from several individuals were stained with TotalSeq-C custom human panel together with Human TruStain FcX (Biolegend. Cat. No. 422301) on ice for 30 minutes ...
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bioRxiv - Immunology 2023Quote: ... B cells were isolated using the EasySep Human B Cell Isolation Kit (StemCell, cat. no. 17954) or the MojoSort Human Pan B Cell Isolation Kit (BioLegend, cat. no. 480082) according to the manufacturer’s instructions.
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bioRxiv - Cell Biology 2020Quote: ... before plating the cells in petri dishes in RPMI 1640 medium containing 10 % heat-inactivated FCS and 50 ng/ml M-CSF (BioLegend) or 50 ng/ml GM-CSF (BioLegend) ...
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Optimized immunoglobulin knock-ins using Cas9 reveal peritoneal B cell lineage relationships in vivobioRxiv - Immunology 2021Quote: Cells were incubated with anti-CD16/32 (Fc block) prior to staining with the following fluorophore or biotin conjugated monoclonal antibodies purchased from Biolegend, eBioscience ...
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bioRxiv - Microbiology 2021Quote: ... Cells were filtered through a 70 µm strainer and red blood cells were lysed before blocking FC receptors using anti-CD16/CD32 (BioLegend). Cell-specific proteins were labeled with the following antibodies ...
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bioRxiv - Microbiology 2021Quote: ... Plates were washed with water three times before applying 25 μL of secondary antibody (goat anti-mouse IgG Fc, BioLegend) at a dilution of 1:4,000 in blocking solution ...
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bioRxiv - Cancer Biology 2020Quote: ... Cells were blocked for 10 min on ice following addition of 10 μL Fc blocking solution (BioLegend, cat. no. 422301), 4 μL of a 1% dextran sulfate solution (Research Products International ...
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bioRxiv - Neuroscience 2021Quote: The myelin-depleted single-cell suspension obtained above was incubated (15 min on ice) with anti-mouse CD16/CD32 antibody to block Fc receptors and subsequently incubated with anti-CD11b PEcy7 (Biolegend), anti-CD45 APC (Biolegend) ...
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bioRxiv - Cell Biology 2021Quote: ... The supernatant was discarded and the cell pellets were resuspended in 10% FCS in PBS containing Alexafluor647 anti-Ki67 (Biolegend; 1:200 in binding buffer ...