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Citations for BioLegend :
151 - 200 of 315 citations for glass bottom plate since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2024Quote: ... and stimulated with plate bound anti-human CD3 Antibody (clone OKT3, 5ng/mL, #317326, Biolegend) and aCD28 (clone CD28.2 ...
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bioRxiv - Bioengineering 2024Quote: ... plates were coated with 2Lμg/ml of a mouse anti-human CD4 antibody (BioLegend, 300502) diluted in PBS overnight at 4 °C ...
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bioRxiv - Systems Biology 2023Quote: ... Plates were coated with 3.33 μg/mL α-CD3 (HITa3) and α-CD28 antibodies (Biolegend), in 100 μL PBS at 4°C overnight ...
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bioRxiv - Immunology 2023Quote: ... 24-well plates have been coated with 1μg purified human TCRγ/δ antibody (331202, BioLegend) per well overnight at 4 °C ...
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bioRxiv - Immunology 2024Quote: Plates for CD8+ T-cell activation were coated with 5μg/mL α-CD3 (Biolegend, 100239) in phosphate buffered saline (PBS ...
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bioRxiv - Immunology 2022Quote: ... PBMCs were activated on plates coated with anti-CD3 (1mg/ml, Biolegend, #317347, clone: OKT3) and anti-CD28 (1mg/ml ...
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bioRxiv - Biochemistry 2022Quote: ... Plates were washed 3X with PBST before adding HRP goat anti-mouse antibody (BioLegend 405306) at a 1:10,000 dilution in PBST ...
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bioRxiv - Immunology 2024Quote: ... After five additional washes the plates were developed by adding the TMB substrate (421501, Biolegend) and read at 620 nm.
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bioRxiv - Immunology 2024Quote: ... and stimulated for 24 h with plate-bound anti-CD3 (5 μg/ml; 2C11; Biolegend) and anti-CD28 (2 μg/ml ...
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bioRxiv - Cancer Biology 2024Quote: ... We then incubated the plates for 30 minutes with 50 µl of HRP Streptavidin (BioLegend) diluted at 1:1000 in blocking buffer at room temperature ...
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bioRxiv - Immunology 2020Quote: ... plates were washed five times with 200µL freshly prepared 0.05% Tween-based ELISA Wash Buffer (BioLegend) and a final time in 200μL phosphate buffered saline (PBS ...
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bioRxiv - Immunology 2020Quote: ... Th2 cells were cultured for 4 days with plate-bound αCD3 (5 μg/ml; Biolegend, #100339), rIL-4 (4 ng/ml ...
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bioRxiv - Immunology 2020Quote: ... Th17 cells were differentiated in the presence of plate-bound αCD3 (5 μg/ml; Biolegend, #100339), rIL-6 (20 ng/ml ...
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bioRxiv - Immunology 2021Quote: 96-well U-bottomed plates were coated with 100μL of 0.5μg/mL streptavidin (BioLegend Cat#270302) at room temperature (18-25°C ...
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bioRxiv - Immunology 2023Quote: ... Plates were washed 4 times with PBS and 40μl of HRP anti-rabbit IgG antibody (Biolegend) 1:1500 was added to each well and incubated for 2 hours at room temperature ...
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bioRxiv - Immunology 2023Quote: ... 96 well plates were coated with purified anti-mouse IgE (2ug/ml, Biolegend, Clone: RME-1) in 0.05M carbonate/ bicarbonate buffer and incubated overnight at 47C ...
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bioRxiv - Immunology 2023Quote: ... 96-well plates were pre-coated with 5 μg/ml anti-hCD3 (BioLegend, Cat# 317326, RRID:AB_11150592), 1 μg/ml anti-hCD28 (Thermo Fisher Scientific ...
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bioRxiv - Immunology 2023Quote: ... plates were washed and the biotinylated anti-mouse IFNγ monoclonal antibody R4-6A2 (Biolegend, 1:2000) was added and incubated for 1 hour at RT ...
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bioRxiv - Immunology 2022Quote: ... The plate was finally washed eight times and was developed with the TMB substrate kit (Biolegend) according the manufacturer’s instructions ...
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bioRxiv - Immunology 2022Quote: ... coated flat bottomed 96 well plates with soluble anti-CD28 (2 μg/mL) (ULTRA-LEAF Biolegend), and with or without human recombinant soluble BAFF at 500 ng/mL ...
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bioRxiv - Microbiology 2022Quote: ... the plates were incubated with biotinylated detection antibodies against IL-12 (Biolegend Cat#505302, clone C17.8) and IL-1β (Biolegend Cat#515801 ...
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bioRxiv - Immunology 2023Quote: ... T cells were activated with 1 μg/ml plate-bound anti-CD3 (BioLegend, clone 145-2C11) and 1 μg/mL anti-CD28 (BioLegend ...
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bioRxiv - Immunology 2024Quote: ... A 24-well non-TC treated plate was coated with 5µg/mL anti-CD3 (BioLegend 100253) overnight at 4C or for 2h at 37C ...
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bioRxiv - Immunology 2024Quote: ... Plates were washed four times with PBS-T and developed by adding TMB substrate (Biolegend, 421101) to each well ...
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bioRxiv - Immunology 2024Quote: ... plates were incubated with biotin-conjugated secondary antibody for 1 hour at RT (BioLegend, catalog 508105). The plates were washed with PBS-T and incubated with Avidin-peroxidase for 30 minutes at RT (Sigma-Aldrich ...
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bioRxiv - Cancer Biology 2022Quote: ... and then plated in a culture plate pre-coated with 10 µg/mL anti-CD3Ɛ antibody (Biolegend). Culture medium was exchanged to R10 containing IL-2 and the cell density was adjusted to be 0.5 – 1x106 cells/ml every 24 h.
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bioRxiv - Immunology 2020Quote: ... mixed LN cells were activated using plate-bound anti-CD3ε (1.5 μg/mL) (2C11, BioLegend, London, UK) and soluble anti-CD28 (37.51 ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... Plates were washed three times with PBS and blocked with Cell Staining Buffer (BioLegend, San Diego, CA). Plates were then incubated with rabbit-anti-LC3B (Cell Signaling Technologies ...
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bioRxiv - Immunology 2020Quote: ... Coated plates were washed and cells were plated with 1 µg/ml soluble αCD28 antibody (37.51, Biolegend). Bone marrow-derived dendritic cells (BMDC ...
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bioRxiv - Immunology 2024Quote: ... were stimulated in a plate coated with LEAF purified anti-mouse CD3ε antibody (clone 145-2111C, BioLegend) and anti-CD28 antibody (clone 37.51 ...
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bioRxiv - Immunology 2024Quote: ... and penicillin in a 96-well plate pre-coated with anti-CD3Ab (2 μg/ml, Biolegend 100360), along with the addition of 5 μg/ml anti-CD28 antibody (Biolegend 102122 ...
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bioRxiv - Immunology 2022Quote: ... the stained cells were distributed across the three 96-well plates provided with the LEGENDScreen kit (Biolegend), each well containing a unique PE-labeled exploratory antibody as well as isotype controls and blanks ...
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bioRxiv - Immunology 2022Quote: ... and 1X nonessential amino acids and stimulated with plate-bound αCD3 mAb (clone HIT3a, BioLegend, cat#300313) at 1 μg/mL and soluble αCD28 mAb (CD28.2 ...
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bioRxiv - Microbiology 2022Quote: ... 96-well plates were coated with primary capture antibody against IL-12 (Biolegend Cat#511802, clone C18.2) and IL-1β (Biolegend Cat#503502 ...
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bioRxiv - Immunology 2023Quote: ... Primary CD4+ T cells were activated in six-well plates coated with anti-CD3e (OKT3; 317326; BioLegend) in presence of soluble anti-CD28 (CD28.2 ...
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bioRxiv - Immunology 2023Quote: 24 well plates were coated with anti-CD3/28 stimulating antibodies (Biolegend Catalog Nos. 100302 and 102102) at final concentration of 5 µg/mL each overnight at 4° C ...
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bioRxiv - Immunology 2024Quote: ... Plates were washed with PBS and coated for >3 h with 1 µg/mL αCD3 (HIT3a, Biolegend) at 37°C ...
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bioRxiv - Immunology 2024Quote: ... Plates for CD8+ T-cell culture were pre-treated with 5 ug/mL α-CD3 (Biolegend, 100239) in phosphate buffered saline for 2 hours at 37°C ...
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bioRxiv - Immunology 2024Quote: ... ELISA plates were coated with purified antibody (3 µg/ml) overnight at 4°C (BioLegend, catalog 519202). The next day ...
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bioRxiv - Cancer Biology 2024Quote: ... Samples were randomized on the assay plates and analyzed using the LEGENDplex human CD8/NK panel (BioLegend). Samples were read on a CytoFLEX S flow cytometer (Beckman Coulter) ...
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bioRxiv - Biochemistry 2024Quote: ... tissue culture plates were coated with 1 µg/mL anti-CD3ε (BioLegend, Ultra-LEAF, Clone 145-2C11) and 0.5 µg/mL anti-CD28 (CyTek in vivo ready ...
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bioRxiv - Immunology 2022Quote: ... 96-well ELISA plates were coated with 3 μg/ml GPI-RBD-GM-CSF in coating buffer (BioLegend) overnight at 4 °C ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... Cytokine levels in macaque plasma were measured using the LEGENDplex NHP Inflammation Panel in filter plates (BioLegend #740332). Plasma samples were diluted 1:4 in assay buffer ...
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bioRxiv - Immunology 2022Quote: ... All cells were differentiated for 48 hours in the presence of plate-bound αCD3 (2.5ug/ml; Biolegend, #100339), rIL-6 (20 ng/ml ...
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bioRxiv - Immunology 2021Quote: ... plates were coated with CD3 antibody (clone OKT3 at 0.5μg/mL) purchased from BioLegend (San Diego, CA USA) 24 hours prior to start of experiment ...
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bioRxiv - Microbiology 2021Quote: ... primary CD4+T cells were activated for 4 days with 1μg/ml plate-bound αCD3 antibody (cloneOKT3, Biolegend) in the presence of 2 μg/mL soluble αCD28 antibody(clone CD28.2 ...
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bioRxiv - Immunology 2022Quote: ... 5x105 cells were plated per well of a 48-well plate coated with CD3 (5 µg/ml) (Biolegend) and cultured in regular T cell media (RPMI 1640 supplemented with 10% heat inactivated FBS (Thermo Fisher Scientific) ...
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bioRxiv - Immunology 2020Quote: ... according to the manufacturer’s protocol and activated in vitro using a combination of plate bound anti-CD3 (2μg/mL) (BioLegend) and soluble anti-CD28 (2μg/mL ...
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bioRxiv - Immunology 2020Quote: ... 2.5 × 105 CTV-labelled T-cells were stimulated with 5µg/ml of plate-bound anti-CD3 (300331, Biolegend) and 5µg/ml of soluble anti-CD28 (302913 ...
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bioRxiv - Cancer Biology 2022Quote: ... from healthy human donor Leukopaks and expanded either on anti-CD3 coated plates (+anti-CD28/IL-2, BioLegend), or in the presence of matched donor-derived monocyte-derived dendritic cells (Wölfl and Greenberg ...