Labshake search
Citations for BioLegend :
1 - 50 of 315 citations for glass bottom plate since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Biochemistry 2022Quote: Flat-bottom 96 well microtitre plates (Nunc MaxiSorp, Biolegend) were coated overnight at 4°C with either r3CLpro-RBD chimer (2 μg/mL) ...
-
bioRxiv - Biochemistry 2022Quote: Flat-bottom 96 well microtitre plates (Nunc MaxiSorp, Biolegend) were coated with r3CL-pro ...
-
bioRxiv - Immunology 2021Quote: ELISA plates (Nunc, MaxiSorp ELISA plates, flat bottom, uncoated, BioLegend, San Diego, CA, USA) were coated with recombinant human CTLA-4-Fc (7268-CT ...
-
bioRxiv - Immunology 2021Quote: 96-well plates (Nunc, MaxiSorp, flat bottom, uncoated, BioLegend, San Diego, CA, USA, 423501) were coated with 0.5 μg/mL rhCTLA-4-Fc chimera (7268-CT ...
-
bioRxiv - Immunology 2024Quote: ... F-bottom 96-wells plate were coated anti-CD3 (10μg/mL, BioLegend, clone OKT3) and rhICAM1-Fc (2μg/mL ...
-
bioRxiv - Microbiology 2024Quote: ... Cytokine levels were determined using the LEGENDplex Mouse Inflammation Panel (13-plex) with V-bottom Plate (BioLegend) according to the manufacturer’s instructions ...
-
bioRxiv - Immunology 2020Quote: ... Cells were placed in V-bottom plates and stimulated with 500X Cell Activation Cocktail with Brefeldin A (Biolegend) in IMDM medium supplemented with 8% FCS ...
-
bioRxiv - Immunology 2023Quote: ... Cells were plated in a 96-well round-bottom plate coated with 3 μg/ml anti-CD3 (Biolegend), uncoated well or with PMA (1 ng/ml ...
-
bioRxiv - Immunology 2024Quote: ... seeded in a F-bottom 96-well plate precoated with 3 µg/mL anti-CD3 (145-2C11; Biolegend) using 5×105 cells (200 µl ...
-
bioRxiv - Immunology 2021Quote: ... and transferred to round-bottom 96-well plates for staining with Aqua live/dead stain (Life Technology or Biolegend), followed by Fc receptor blocking (normal immunoglobulin ...
-
bioRxiv - Immunology 2021Quote: ... 1×106 splenocytes were isolated from mice and cultured on Immulon U-bottom plates coated with antibodies for Ly49D (0.5mg/ml) (Biolegend) or NKp46 (1mg/ml ...
-
bioRxiv - Immunology 2022Quote: Plates (48- or 96-well, flat bottom) were coated with 10 μg/ml αCD3ε mAb (BioLegend, San Diego, CA) and incubated at 37°C for 5 hours ...
-
bioRxiv - Immunology 2024Quote: ... at a 1:1 ratio at 37C with 5% CO2 in a U-bottom 96-well plate in 200µL of T cell media with 10µL of anti-mouse CD107a-BV421 (BioLegend 121617). After 1 hour ...
-
bioRxiv - Cancer Biology 2021Quote: 96-well round-bottom plates were coated with either 2.5 µg/mL anti-CD3 (Biolegend, clone 145-2C11,Cat# 100359) or HIgG (Biolegend ...
-
bioRxiv - Genomics 2020Quote: ... 1×105 TILs were seeded per well of a round bottom 96 well plate and stimulated with phorbol-12-myristate-13-acetate/Ionomycin in the presence of Brefeldin A (Biolegend) for 4 hours as per manufacturer’s instruction ...
-
bioRxiv - Immunology 2020Quote: ... The cells were activated in 96-well round bottom plates that were coated with 3ul/ml anti-CD3e (BioLegend #100202) and 5ul/ml anti-CD28 (BioLegend #102102 ...
-
bioRxiv - Immunology 2021Quote: ... 0.05 mM β-mercaptoethanol) on a 96-well flat bottom plate coated with anti-CD3 antibody (10µg/ml, cat. # 317325, BioLegend). 7 x 103 DC3 or 5 x 104 monocytes were used per well ...
-
bioRxiv - Immunology 2021Quote: ... sort-purified γδ or αβ IELs were seeded at approximately 400K cells per well in round-bottom 96-well plates coated overnight with 2 μg/ml anti-CD3 (145-2C11, BioLegend), 2 μg/ml polyclonal Armenian hamster IgG (BioXCell) ...
-
bioRxiv - Immunology 2023Quote: ... Cells were activated for 3 h in a round-bottom 96-well plate using Cell Activation Cocktail (with Brefeldin A) (BioLegend). Plates were then centrifuged at 300 x g for 5 min and the pellets resuspended in 50 µL of Cell-ID™ Cisplatin-195Pt viability reagent (Standard BioTools) ...
-
bioRxiv - Genomics 2022Quote: ... flat bottom 96-well plates were coated with 2 μg/mL of anti-CD3 (Clone: 145-2C11, BioLegend, Cat#100302) in PBS overnight at 4°C or 4 hrs at 37°C ...
-
bioRxiv - Microbiology 2023Quote: ... 100,000 cells were plated in 96-well round bottom plate and blocked with 1:1000 Human TruStain FcX™ (Biolegend) diluted in washing buffer for 10 minutes ...
-
bioRxiv - Immunology 2024Quote: ... 0.3 x 106 OT-I T cells were placed on flat-bottom 96 well plates coated with 5 µg/mL anti-CD3ε (clone 145-2C11; Biolegend) and stimulated with 1 µg/mL anti-CD28 mAb (clone 37.51 ...
-
bioRxiv - Immunology 2024Quote: ... F-bottom 96-wells plate were coated increasing anti-CD3 concentrations (ranging from 0.001 to 10μg/mL, BioLegend, clone OKT3) overnight at 4°C ...
-
bioRxiv - Immunology 2022Quote: ... 50 μl of supernatants from cultures of T cell clones were collected after 48 hrs of stimulation and added to each well of 96-well round-bottom plates precoated with IFN-γ (clone MD-1) capturing antibodies (BioLegend). After overnight incubation ...
-
bioRxiv - Immunology 2022Quote: ... 200,000 cells were plated in a 96-well round bottom plate and blocked with 1:1000 Human TruStain FcX™ (Biolegend) for 10 minutes ...
-
bioRxiv - Immunology 2024Quote: ... The LEGENDplex™ Mouse Immunoglobulin Isotyping Panel catalog #740493 with the V-bottom plate was used according to manufacturer’s instructions (BioLegend, CA). Briefly ...
-
bioRxiv - Immunology 2023Quote: High-binding 96-well flat-bottom plates were coated with 100 μl per well of 20 μg/ml anti-NK1.1 (Clone PK136, BioLegend, cat. 108759) in PBS at 4 °C overnight ...
-
bioRxiv - Immunology 2023Quote: ... LN single cell suspensions were stimulated in a 96-well round-bottom plate with Cell Stimulation Cocktail containing PMA and ionomycin (eBioscience 00-4970-03, Thermo Fisher or 423302, BioLegend) at 37 °C for 1 hour ...
-
bioRxiv - Bioengineering 2024Quote: ... Supernatants were collected on day 6 and immediately assessed for cytokine and chemokine secretion by LEGENDplex™ using LEGENDplex Human Inflammation Panel 1 (13-plex) with V-bottom Plate (Biolegend catalog #740809 ...
-
bioRxiv - Immunology 2024Quote: ... T cells were cultured in a 96-well round bottom plate at the density of 50,000 cells/200 µL with 100 U/mL IL-2 (BioLegend, Cat. 589104) for 96 hours ...
-
bioRxiv - Immunology 2024Quote: ... Cells were cultured in a 96-well round bottom plate at a density of 50,000-600,000 cells/300 µL with 100 U/mL IL-2 (Biolegend, Cat. 575404 (mouse) 589104 (human)) ...
-
bioRxiv - Immunology 2022Quote: Activated γδT cells were seeded in round-bottom plates with FITC conjugated anti-mouse CD107a (1D4B) antibody or anti-human CD107a (H4A3) (Biolegend, San Diego, CA). γδT cells were co-cultured with A20 or K562 at a ratio of 1:1 ...
-
bioRxiv - Immunology 2024Quote: ... and 50,000 cells were added to each well of a 96-well U-bottom plate to provide APC-mediated stimulation alongside soluble 11-CD3 (BioLegend; 0.5 µg/mL) and 11-CD28 (BioLegend ...
-
bioRxiv - Immunology 2021Quote: ... CD8+ T cells isolated at each time point of interest were directly stained in round-bottom 96-well plates using the following surface-marker antibodies (either from eBioscience, BioLegend, or produced in house): anti-mouse CD8a FITC (clone 53.6.7 ...
-
bioRxiv - Immunology 2021Quote: ... The resulting whole-splenocyte single-cell suspensions were directly stained in round-bottom 96-well plates using the following surface-marker antibodies (either from eBioscience, BioLegend, or produced in house): anti-mouse CD8a FITC (clone 53.6.7 ...
-
bioRxiv - Immunology 2023Quote: Levels of murine cytokines in transplanted mice were assessed using the LEGENDplex™ Murine Inflammation panel (13-plex) in a V-bottom plate per manufacturer’s instructions (Cat. No: 740446, BioLegend, San Diego, CA, USA). LEGENDPlex data were acquired on a BD Fortessa analyzer (BD Biosciences ...
-
bioRxiv - Immunology 2022Quote: Supernatants were assessed for cytokine production using the LEGENDplex™ Human Inflammation Panel 1 (13-plex) in a V-bottom plate per the manufacturer’s instructions (BioLegend, San Diego, CA, USA). Flow cytometry data were acquired on a BD Fortessa analyzer (BD Biosciences ...
-
bioRxiv - Immunology 2021Quote: ... were coated overnight at 4°C with 50 µL/well of either 10 µg/mL (flat-bottom) or 5 µg/mL (round-bottom) anti-mouse CD3ε (BioLegend) in PBS (for stimulated wells) ...
-
bioRxiv - Immunology 2022Quote: ... ELISA plates (Immulon 2HB 96-well plates, Biolegend) were coated with 100 ng per well (100 μl ...
-
bioRxiv - Immunology 2024Quote: ... with the bottom chamber containing either 0 or 100 ng/mL CCL20 (BioLegend) in 0.6 mL complete media ...
-
bioRxiv - Pathology 2021Quote: ... Microwell plates (BioLegend, #423501) were coated with purified GM-CSF capturing antibody from ELISA MAX™ Deluxe Set Mouse GM-CSF (Biolegend) ...
-
bioRxiv - Bioengineering 2020Quote: Maxisorp plates (BioLegend, 423501) were coated with 1μg/ml anti-Flag antibody (Sigma Aldrich ...
-
bioRxiv - Immunology 2020Quote: ... cells migrated to the bottom chamber were collected and stained with CD8 AF700 (BioLegend) and resuspended in 200 μl of PBS ...
-
bioRxiv - Immunology 2022Quote: 96-well ELISA plates (BioLegend) were coated with 50 μL of Ovalbumin from chicken egg white (Sigma-Aldrich ...
-
bioRxiv - Cancer Biology 2022Quote: ... The bottom chamber was also supplemented with either vehicle or CXCL10 (500 ng/mL, BioLegend) to promote T cell migration ...
-
bioRxiv - Pathology 2022Quote: An uncoated ELISA plate (423501, Biolegend) was coated with 1.2 μg/mL HERV-K113 Capsid protein (sp|Q902F9|90-632 ...
-
bioRxiv - Immunology 2024Quote: ... Nunc MaxiSorp 96-well plates (BioLegend) were coated with an anti-IL-12p40 capture antibody (2 μg/ml ...
-
bioRxiv - Immunology 2022Quote: ... or plate-bound α-CD3ε (2 μg/mL) and plate-bound CD155-Fc (1 μg/mL; BioLegend) for four days ...
-
bioRxiv - Cancer Biology 2022Quote: ... plate-bound OKT3 (1 μg/mL; Biolegend) was used as a positive control ...
-
bioRxiv - Bioengineering 2023Quote: ... Nunc MaxiSorp ELISA plates (Biolegend, Cat# 423501) were coated with 10μg/ml trimeric spike protein overnight at 4°C ...