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Citations for BioLegend :
101 - 150 of 315 citations for glass bottom plate since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2024Quote: ... and plate-bound 11-CD28 (BioLegend; RRID: AB_11147170; 1 µg/mL) or plate-bound 11-CD3 (BioLegend ...
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bioRxiv - Immunology 2024Quote: ... Plates were washed four times with washing buffer (BioLegend; cat# 4211601) and then incubated for 1 h at RT with horseradish peroxidase (HRP)-conjugated anti-mouse secondary antibodies (1:2000 dilution ...
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bioRxiv - Cell Biology 2022Quote: ... poly-l-lysine coated glass slides were coated by overnight incubation with ICAM-1 (5 μg/mL, Biolegend 552906) or with ICAM-1 and anti-CD28 (5 μg/mL ...
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bioRxiv - Immunology 2021Quote: ... plates were further processed with anti-mouse IgG1 (clone RMG1-1, BioLegend) in 1:1000 dilution for 2 hours.
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bioRxiv - Immunology 2021Quote: ... Plates were washed five times and developed with tetramethylbenzidine substrate (Biolegend, 421101). The reaction was stopped with 1 M phosphoric acid ...
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bioRxiv - Biochemistry 2020Quote: ... and plate-bound Armenian hamster anti-mouse CD3ε (clone 145-2C11, BioLegend, USA ...
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bioRxiv - Immunology 2022Quote: ... Plates were washed five times and developed with tetramethylbenzidine substrate (Biolegend, 421101). The reaction was stopped with 1 M phosphoric acid after 20 min at room temperature ...
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bioRxiv - Microbiology 2020Quote: ... cell culture plates were pre-coated with anti-mouse CD3 antibody (Biolegend) (1 μg/ml ...
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bioRxiv - Immunology 2021Quote: ... in the presence of plate- bound anti-CD3 (5 μg/mL, Biolegend), CpG (10 μg/mL ...
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bioRxiv - Immunology 2020Quote: ... The plates were washed and developed with 50 µl TMB (Biolegend, UK); after 5 minutes the reaction was stopped with 50 µl 1M sulfuric acid and absorbance measured at 450 and 630 nm ...
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bioRxiv - Neuroscience 2021Quote: ... and then incubated on a plate coated with anti-CD140a (#135902, Biolegend) for OPC selection ...
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bioRxiv - Cancer Biology 2024Quote: ... plates were coated with 10 μg/ml anti-CD3 (145-2C11, Biolegend) for 2 h at 37 °C ...
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bioRxiv - Immunology 2024Quote: ... ELISPOT plates were coated with capture α-IFNγ (clone R4-6A2, Biolegend) and subsequently blocked ...
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bioRxiv - Immunology 2022Quote: ... plates were coated with brain lysates in an ELISA Coating Buffer (Biolegend) instead of mouse or human IgG antibodies.
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bioRxiv - Microbiology 2020Quote: ... The cell culture plates were pre-coated with anti-mouse CD3 antibody (Biolegend) (1 μg/ml) ...
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bioRxiv - Immunology 2020Quote: ... T cell activation was performed using plate-bound α-CD3 (clone OKT3, Biolegend) and α-CD28 (clone 9.3 ...
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bioRxiv - Immunology 2022Quote: ... T cells were activated with plate-bound anti-CD3ε (clone 145-2C11; Biolegend) and 0.5 µg/ml of soluble anti-CD28 (clone 37.51 ...
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bioRxiv - Immunology 2023Quote: ... stimulated with 1 μg/ml of plate-bound anti-CD3 (145-2C11, Biolegend), 2 μg/ml anti-CD28 (37.5 ...
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bioRxiv - Bioengineering 2024Quote: ... Plates were then washed with PBS before the addition of 1x streptavidin (Biolegend) in PBS + 0.5% BSA (w/v ...
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bioRxiv - Bioengineering 2024Quote: ... and then activated by pre-coated plate-bound anti-CD3 (1μg/ml; Biolegend) and soluble anti-CD28 ((1μg/ml ...
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bioRxiv - Immunology 2024Quote: ... plates were coated with 2 µg/ml anti-mouse-IgM or IgG (Biolegend) in NaHCO3 0.1 M pH 9.6 ...
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bioRxiv - Neuroscience 2022Quote: ... cells were restimulated on plates coated with anti-CD3 and anti-CD28 (BioLegend). After 48 hours of restimulation ...
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bioRxiv - Immunology 2022Quote: ... TCR stimulation was provided by plate-bound αCD3 (clone HIT3a, BioLegend, cat#300313) at 1μg/mL unless otherwise specified ...
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bioRxiv - Immunology 2022Quote: ... plates were washed and incubated with 100 μL/well TMB-substrate (Biolegend/ITK). The substrate reacted approximately 8 minutes ...
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bioRxiv - Immunology 2024Quote: ... Plates were coated with anti-IL2 antibodies ((mouse: clone JES6-1A12, BioLegend, 503701) (human ...
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bioRxiv - Cancer Biology 2022Quote: ... 96-well plates were coated with 5 g/ml of anti-CD28 antibody (Biolegend) o/n at 4°C to provide a co-stimulatory signal during co-culture ...
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bioRxiv - Immunology 2022Quote: ... Tissue and serum samples were loaded directly onto ELISA plates according to protocol (Biolegend 439407 Legend Max Mouse IFN-B ELISA kit) ...
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bioRxiv - Immunology 2022Quote: ... After the plate was washed five times and a 3,3′,5,5′-Tetramethylbenzidine solution (Biolegend) was prepared and incubated for 30 min ...
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bioRxiv - Microbiology 2021Quote: ... Purified CD4+ T cells were co-stimulated with plate-bound CD3 antibody (Biolegend #300333) with media containing soluble CD28 (Biolegend #302943 ...
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bioRxiv - Immunology 2021Quote: ... cells were cultured overnight with plate-bound anti-CD3 (OKT3, 1 μg/ml, Biolegend) and soluble anti-CD28 (CD28.2 ...
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bioRxiv - Immunology 2020Quote: ... - plates were coated with Goat anti-mouse IgG (clone Poly4053 at 10μg/ml - BioLegend) for 1 hour at 37°C ...
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bioRxiv - Immunology 2023Quote: ... the plate was further incubated with HRP-conjugated goat anti-mouse IgG (poly4053, Biolegend) at 1:20,000 for 1 h before development with TMB Substrate Set (421101 ...
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bioRxiv - Immunology 2022Quote: ... in PBS were added to 96-well high-bound Nunc MaxiSorp ELISA plates (BioLegend) and incubated at 4°C overnight ...
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bioRxiv - Immunology 2024Quote: ... plates were coated with 2 µg/mL of anti-IgE (clone RME-1, BioLegend) and for IgG1 ELISAs ...
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bioRxiv - Immunology 2024Quote: Plates were coated with 1.25 µg/ml anti-CD3 (145-2C11 or OKT3, Biolegend) with or without 10 µg/ml Fc proteins (R&D Systems Inc.) ...
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bioRxiv - Immunology 2023Quote: ... T cells were incubated in 96 well plates with Brefeldin A (15µg/mL, Biolegend) and stimulated with PMA (5ng/mL ...
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bioRxiv - Developmental Biology 2022Quote: ... Plates were coated with recombinant human E-cadherin-Fc chimera (BioLegend #BLG-779904-25ug) to reduce colony formation and thereby limit the potential impact of paracrine and direct cell signaling in regulating gene expression of related cells 50 ...
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bioRxiv - Immunology 2024Quote: ... 1 million naïve CD8+ T-cells were activated with plate-bound anti-CD3 (Biolegend Ultra-LEAF purified ...
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bioRxiv - Immunology 2024Quote: ... The signals from the microplate plate were visualized using the TMB substrate solution (BioLegend) and read at 650 nm
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bioRxiv - Microbiology 2024Quote: ... plated in 24-well plates coated with 2µg/mL of anti-CD3 antibody (Biolegend), and incubated at 37°C and 5% CO2 for 4 days ...
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bioRxiv - Immunology 2022Quote: ... tissue culture plates were coated with 5 μg/mL of anti-human CD162 (KPL, Biolegend). On day three of activation ...
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bioRxiv - Immunology 2021Quote: ... Plates were washed three times and incubated ∼7min with 50 µl of TMB substrate (BioLegend). The reaction was stopped with 25µl of 1M phosphoric acid (Sigma ...
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bioRxiv - Cancer Biology 2022Quote: ... 96-well plates were coated in anti-CD3 antibody (1 μg/ml Biolegend, cat #100340), and splenocytes from CD-1 nude mice were added as antigen presenting cells (APCs) ...
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bioRxiv - Immunology 2020Quote: ... CD4+ T cells were stimulated with plate bound αCD3 and αCD28 (both 1μg/ml, Biolegend). After 12 hours ...
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bioRxiv - Immunology 2021Quote: ... Cells were activated in a 24-well plate coated with anti-CD3 (clon: 500A2, Biolegend) and soluble anti-CD28 (clon ...
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bioRxiv - Immunology 2020Quote: ... tissue-culture plates were coated with 5 μg/mL purified anti-CD3 (clone HIT3a, Biolegend) at 4°C overnight and subsequently washed twice with 1X PBS ...
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bioRxiv - Immunology 2020Quote: ... Plates were washed and 50 μl/well of TMB High Sensitivity substrate solution (BioLegend, UK) was added ...
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bioRxiv - Cancer Biology 2021Quote: ... and cultured in 96-well plates coated with 1µg/ml anti-CD3 (BioLegend, Clone UCHT1) or control mIgG (BioLegend ...
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bioRxiv - Immunology 2023Quote: ... The 96-well ELISA plates were pre-coated with purified anti-mouse IL-2 (BioLegend) using coating buffer (0.1 M Na2HPO ...
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bioRxiv - Immunology 2022Quote: ... The plate was washed five times with PBST and developed with tetramethylbenzidine substrate (Biolegend, 421101). After 20 min at room temperature ...