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Citations for BioLegend :
1901 - 1950 of 9012 citations for 6H Pyrazolo 3 4 b pyridin 6 one 3 cyclobutyl 1 2 4 5 tetrahydro 4 methyl 9CI since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2021Quote: ... with the caveat that CD8+ enrichment was achieved by incubating 1-2 million PBMCs with CD8 Mojobeads (Biolegend 480108) for 15 minutes on ice ...
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bioRxiv - Immunology 2024Quote: ... then after 2 washes cells were incubated with streptavidin (SA) conjugated to Brilliant Violet 650 (BV650, 1:200, BioLegend) in PBS on ice for 15 minutes ...
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bioRxiv - Cell Biology 2024Quote: ... Membranes were blocked overnight in Odyssey PBS Blocking Buffer (Li-Cor) and stained for 2 hours at RT with Syn1 (1:1,000; Biolegend) or γH2AX (1:1,000 ...
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bioRxiv - Immunology 2021Quote: ... cells were permeabilized with 0.5% saponin for 15 min and intracellularly stained with PE-anti-perforin (clone B-D48) antibody (Biolegend) for 20 minutes ...
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bioRxiv - Pathology 2022Quote: ... or B cell supernatant pre-treated with a recombinant antibody to deplete IL-10 (IgG2b, clone JES5-16E3, Biolegend). As controls ...
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bioRxiv - Immunology 2022Quote: ... B cell purity was > 95% based on flow cytometric analysis following staining with anti-CD19 (Biolegend, San Diego, CA). FO and MZ B cells were >90% pure based on the criteria that they were sorted on.
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bioRxiv - Immunology 2023Quote: ... This reference curve was established in each experiment for all FcyR-specific antibodies of interest (PE-conjugated clone 10.1 to detect FcyRI, clone AT10 to detect FcyRIIa/b and clone 3G8 to detect FcyRIIIa, all from Biolegend) and used to calculate receptor numbers based on fluorescence intensity of FcyR staining on CHO cells ...
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bioRxiv - Cancer Biology 2023Quote: ... IL-1β (432604) and Cathepsin B (EA100429) enzyme-linked immunosorbent assay (ELISA) kits were from BioLegend (San Diego, CA) and Origene (Rockville ...
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bioRxiv - Immunology 2023Quote: ... followed by 100ul of Streptavidin nanobeads for 1×10^8 cells in 1ml of MACS buffer for 15min on ice (MojoSort Mouse pan B cell isolation kit II, Cat.no. 480088, Biolegend). The cell suspension was diluted and put on a pre-rinsed LS Column (Cat ...
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bioRxiv - Immunology 2021Quote: ... in PBS with 2% FCS and 2% EDTA (FACS buffer) with Fc block (Biolegend) for 25 minutes ...
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bioRxiv - Immunology 2023Quote: ... and 2 ng/ml human interleukin (IL)-2 recombinant (BioLegend, San Diego, CA, 589104).
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bioRxiv - Immunology 2023Quote: ... IL-2 concentration in thawed supernatant was measured by ELISA (Biolegend, IL-2 human). For experiments with KIR2DS1 reporter cells ...
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bioRxiv - Microbiology 2019Quote: ... or double knockout cells were harvested and washed with PBS containing 1% bovine serum albumin (BSA) and incubated with 5 μL of PE-conjugated EphA2 antibody (Biolegend, SHM16) in 50 μL PBS containing 1% BSA for 30 minutes at 4 °C ...
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bioRxiv - Microbiology 2022Quote: ... bacteria were labeled with 5 µg ml−1 carboxylfluorescein diacetate succinimidyl ester (CFSE) or Tag-IT Violet™ proliferation and cell tracking dye (Biolegend) in PBS containing 5 mM MgSO4 ...
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bioRxiv - Immunology 2022Quote: ... single-cell spleen suspensions from C57BL/6J mice were cultured at 5 million cells/ mL in complete RPMI with 1 μg/mL anti-CD3 (BioLegend, #100340) and 1 μg/mL anti-CD28 (BioLegend ...
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bioRxiv - Neuroscience 2023Quote: ... Membranes were incubated in primary antibodies diluted in 5% skim milk in TBST: mouse anti-brevican (1:1000, BioLegend, cat# 820101), sheep anti-neurocan (1:1000 ...
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bioRxiv - Neuroscience 2022Quote: ... Then the live microglial cells were incubated with PBS supplemented with 5% BSA + HLA-DR mouse monoclonal antibodies pre-conjugated with FITC (1:100, BioLegend, Inc.) + Hoechst 33342 (1:5000 ...
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bioRxiv - Synthetic Biology 2022Quote: ... The membrane was incubated with 5% nonfat milk in TBST for 1 hour followed by the addition of primary antibodies (BioLegend, 652505). After 1 hour of the primary antibody incubation ...
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bioRxiv - Synthetic Biology 2022Quote: ... The membrane was incubated with 5% nonfat milk in TBST for 1 hour followed by the addition of primary antibodies (BioLegend, 652505). After 1 hour of the primary antibody incubation ...
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bioRxiv - Immunology 2024Quote: The Fc receptors were blocked on 5X105 neutrophils resuspended in PBS with 5% FBS and TruStain FcX (1:50, BioLegend, 101320). Neutrophils were incubated with FITC conjugated anti-mouse Ly6G (1:50 ...
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bioRxiv - Microbiology 2024Quote: ... Cells were pelleted by centrifugation (2,000 rpm 5 min) and then treated with 1 mL 1X red blood cell (RBC) Lysis Buffer (Biolegend; cat# NC9067514) to lyse red blood cells ...
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bioRxiv - Immunology 2024Quote: ... diluted 1:500 in 1x Permeabilisation buffer with 5% Normal Donkey Serum at +40C and 1 h staining with DyLightTM 649 Donkey anti-rabbit IgG (BioLegend, #406406) diluted 1:500 in 1x Permeabilisation buffer ...
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bioRxiv - Immunology 2021Quote: ... Anti-IL-6 (MP5-20F3) and anti-TNF-alpha (MP6-XT22) were from BioLegend.
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bioRxiv - Physiology 2019Quote: ... and hyaluronan were measured using commercial enzyme-linked immunosorbent assay kits (IL-6: BioLegend, San Diego ...
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bioRxiv - Cancer Biology 2019Quote: ... and plated for activation on 6 well plates previously coated with αCD3 antibody (BioLegend, CA ...
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bioRxiv - Cancer Biology 2022Quote: ... and with or without anti-IL-6 antibody (20 μg/ml, Biolegend Cat#504512). Cells were then harvested from plates ...
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bioRxiv - Cancer Biology 2022Quote: ... ELISA was conducted using the Mouse IL-6 ELISA Max™ Delux kit (Biolegend), following the manufacturer’s instructions ...
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bioRxiv - Immunology 2024Quote: IL-6 ELISA was performed according to the manufacturer’s instruction (Biolegend, Cat. No. 431301) with mouse serum diluted 1:50 ...
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bioRxiv - Immunology 2023Quote: ... in the presence or absence of 20ng/ml of recombinant mouse IL-6 (Biolegend) and/or 200ng/ml of nivalenol (Cayman Chemical ...
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bioRxiv - Bioengineering 2023Quote: ... and values were calculated based on the standard curve for IL-6 (BioLegend 431315), IL-1β (BioLegend 432615) ...
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bioRxiv - Cell Biology 2020Quote: ... Diamidino-2-Phenylindole (DAPI) (Biolegend) for live/dead exclusion and cell sorting ...
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bioRxiv - Immunology 2021Quote: ... IL-2 (MQ1-17H12, Biolegend), and IFNγ (4S.B3 ...
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bioRxiv - Immunology 2021Quote: ... and 2 μM monensin (BioLegend). For intracellular staining of IgM ...
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bioRxiv - Cancer Biology 2022Quote: ... and monensin (2 μM, Biolegend) in a humidified incubator at 37°C with 5% CO2 ...
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bioRxiv - Bioengineering 2020Quote: ... beta-2-microglobulin (BioLegend #316306) and Fixable Viability Dye eFluor780 or LIVE/DEAD Fixable Aqua Dead Cell Stain (ThermoFisher #65-0865-14 1:500 dilution ...
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bioRxiv - Immunology 2022Quote: ... and 2 nM Monensin (BioLegend) were added to each well and incubated for further 2 h ...
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bioRxiv - Immunology 2019Quote: ... CD138(BV421, 281-2, BioLegend, #142507 ...
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bioRxiv - Immunology 2020Quote: ... anti-CD138 (281-2, Biolegend), anti-IgD (11-26c.2a ...
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bioRxiv - Genomics 2021Quote: ... IL-2 (Biolegend, Cat #500310), or CTLA-4 (Biolegend ...
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bioRxiv - Genomics 2021Quote: ... IL-2 (Biolegend, Cat #500310), or CTLA-4 (Biolegend ...
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bioRxiv - Immunology 2020Quote: ... 2) huCD45 APC Cy7 (BioLegend), huCD3 PerCp-Cy5.5 (BioLegend) ...
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bioRxiv - Immunology 2020Quote: ... CD138 (clone 281-2, Biolegend), B220 (clone RA3-6B2 ...
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bioRxiv - Cancer Biology 2020Quote: ... CD138 (BioLegend; clone: 281-2) and analyzed for germinal center activated B cells (GCB) ...
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bioRxiv - Immunology 2020Quote: ... IL-2 PE (Biolegend 503808), CD4 PE-Cy7 (Biolegend 100528) ...
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bioRxiv - Immunology 2020Quote: ... IL-2 BV605 (Biolegend 500332), and Live/dead Near-IR (Invitrogen L10119) ...
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bioRxiv - Immunology 2021Quote: ... or -BV510 (281-2, Biolegend) and B220-BV421 (RA3-6B2 ...
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bioRxiv - Immunology 2022Quote: ... CD49d-FITC (R1-2, BioLegend), Tbet-APC (4B10 ...
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bioRxiv - Immunology 2024Quote: ... and monensin (2 µM; Biolegend). Phorbol 12-myristate13-acetate (PMA)/ionomycin-stimulated (Cell Activation cocktail ...
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bioRxiv - Pathology 2023Quote: ... CD206 (Biolegend; clone 15-2) and CD163 (Biolegend ...
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bioRxiv - Cell Biology 2023Quote: ... mouse CD63 (NVG-2, Biolegend), CD9 (312102 ...