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Citations for BioLegend :
2051 - 2100 of 9012 citations for 6H Pyrazolo 3 4 b pyridin 6 one 3 cyclobutyl 1 2 4 5 tetrahydro 4 methyl 9CI since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2022Quote: ... Approximately 5×105 cells were stained for 30 min using the Zombie Aqua fixable viability dye (BioLegend Cat# 423101, 1:500 dilution), then fixed for 1 h in Foxp3/Transcription Factor Fixation/Permeabilisation Solution (ThermoFisher Cat# 00552300) ...
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bioRxiv - Immunology 2024Quote: ... Primary human CD4+ T cells from CTLA4-deficient patients or healthy controls were purified from 5 million frozen PBMCs using a biotin labeled anti-human CD4 antibody (OKT4; BioLegend; 1:20) followed by magnetic separation with anti-biotin microbeads (Miltenyi Biotic ...
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bioRxiv - Microbiology 2023Quote: ... Primary antibodies were resuspended in 0.1% Tween-20 in PBS supplemented with 5% BSA at the following dilutions: TUJ1 (BioLegend, 801202, 1:400), SOX2 (Invitrogen ...
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bioRxiv - Immunology 2021Quote: ... The splenocytes were incubated for 6 hr at 37°C in vitro with BrefeldinA (Biolegend) under three conditions ...
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bioRxiv - Neuroscience 2022Quote: Enzyme-linked immunosorbent assays were performed using the mouse IL-6 ELISA-MAX kit (Biolegend), using manufacturer’s instructions.
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bioRxiv - Cancer Biology 2022Quote: NSG mice were administrated intraperitoneally with IL-6 (50 μg /mouse) neutralizing Ab (BioLegend 504512) for two days ...
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bioRxiv - Immunology 2020Quote: ... and then stimulated with 10nM HyIL-6 or 0.1 μg/mL anti-CD3 (Biolegend #300438) and 20 ng/mL IL2 (Novartis #709421) ...
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bioRxiv - Immunology 2021Quote: ... and supernatants were harvested for IL-6 and IL-1β ELISA (Biolegend, San Diego, CA). BMDCs were also utilized in the MLR and in vitro T cell differentiation assays.
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bioRxiv - Immunology 2022Quote: ... and then stained with PE-conjugated anti-mouse IL-6 mAb (clone MP5-20F3, Biolegend). Stained cells were analysed using a BD LSR Fortessa cell analyser (BD Biosciences ...
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bioRxiv - Immunology 2024Quote: WT C57Bl/6 mice were injected intraperitoneally with 250 ng recombinant mouse IL-33 (Biolegend) in 100 µl PBS for three consecutive days ...
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bioRxiv - Cancer Biology 2023Quote: ... and PE anti-mouse H-2Kb/H2Db antibody (Clone 28-8-6; Biolegend; Cat #114607) on ice for 20 minutes in the dark ...
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bioRxiv - Microbiology 2024Quote: ... IL-6 and TNF-α ELISA kits according to the manufacturer’s instructions (BioLegend, CA, USA).
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bioRxiv - Immunology 2019Quote: ... 105 resting NK cells were mixed at 1:1 with 221 cell lines or peptide loaded target cells in presence of 1 μl BV421 anti-CD107a mAb for 2 hr at 37°C (H4A3, Biolegend 328626). NK cells were then stained with mAbs to identify subsets based on the expression of KIR2DL1/S1 (Beckman Coulter ...
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bioRxiv - Immunology 2020Quote: ... were added to the tumor cells and incubated for 2 hours with CD20-TCB (200 ng/ml) and +/- αLFA1 antibody (Clone: TS 1/18, 10 μg/ml, BioLegend, 302116). Cells were then gently washed ...
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bioRxiv - Immunology 2019Quote: ... Antibody coated cells were mixed with resting NK cells in the presence of 1 μl BV421 anti-CD107a mAb for 2 hr at 37°C (H4A3, Biolegend 328626). Cells were then stained with mAbs to KIR2DS4 (Miltenyi Biotec ...
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bioRxiv - Immunology 2021Quote: ... Approximately 2 million cells per experimental condition were stained with 1 μg of TotalSeq™ B0301-B0308 anti-mouse Hashtag antibodies (BioLegend) according to the manufacturer’s instructions and pooled together at equal cell numbers ...
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bioRxiv - Immunology 2024Quote: ... Cells were then washed with FACS buffer (PBS+ 2% (v/v) FBS with 1mM EDTA) and incubated with a 1:500 dilution of TruStain FCX (Biolegend, 101320) for 5 minutes ...
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bioRxiv - Cell Biology 2024Quote: ... and the resulting suspension was plated at 1-2×106 cells/ml in complete medium containing 20% FCS and 20 ng/ml M-CSF (BioLegend, 574804). Cells were incubated for 6 days with a medium replacement at day 3 ...
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bioRxiv - Immunology 2023Quote: ... For the memory B cell EliSpot, PBMC were stimulated with 1 µg/ml R848 (SigmaAldrich, SML0196) and 10 ng/ml recombinant human IL-2 (Biolegend, 791904) for 5 days before harvesting and plating of 50-100.000 cells/well ...
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bioRxiv - Immunology 2021Quote: ... anti-CD138 (clone 281-2, Biolegend). Specific conjugates are indicated within Figures ...
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bioRxiv - Immunology 2021Quote: ... and Thy1/2 (Biolegend, 30-H12) conjugated to APC-Cy7 each at a concentration of 0.25 μg per 1 x 106 cells to remove dendritic cells ...
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bioRxiv - Immunology 2021Quote: ... IL-2 (20 ng/ml; BioLegend), IL-4 (20 ng/ml ...
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bioRxiv - Immunology 2022Quote: ... IL-2 (clone: JES6-5H4; BioLegend) and IL-17A (clone ...
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bioRxiv - Immunology 2022Quote: ... and Monensin solution (2 μM) (BioLegend). Cells were then stained with one of four antibody panels (Supplementary Table 1 ...
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bioRxiv - Immunology 2020Quote: ... anti-IL-2-PE-Cy7 (Biolegend) and anti-IL-4-PE (Biolegend) ...
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bioRxiv - Bioengineering 2021Quote: ... αCD4 mAb (clone RM4-2, Biolegend), anti-Camelid VHH antibody (clone 96A3F5 ...
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bioRxiv - Bioengineering 2021Quote: ... αCD4 mAb (clone RM4-2, Biolegend), αCD11b mAb (clone M1/70 ...
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bioRxiv - Immunology 2020Quote: ... α-BCL-2 (BCL/10C4, 633510, Biolegend), α-BIM (K.912.7 ...
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bioRxiv - Immunology 2020Quote: Panel 2: CD11b-BV421 (Biolegend, 301323), CD274-PE (Biolegend ...
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bioRxiv - Immunology 2020Quote: ... anti-SDC1-PE (281-2, BioLegend), and anti-FR4-PE Cy7 (ebio12A5 ...
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bioRxiv - Immunology 2021Quote: ... IgD PE-Cy7 (IA6-2, BioLegend) Zombie NIR (BioLegend ...
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bioRxiv - Immunology 2021Quote: ... IgD PE-Cy5 (IA6-2, BioLegend), CD14 PerCP (HCD14 ...
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bioRxiv - Immunology 2022Quote: ... IL-2 BV421 (Biolegend, cat.503826), IL-4 PE (Biolegend ...
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bioRxiv - Immunology 2022Quote: ... α-CD8b.2 APC (53-5.8, BioLegend) and α-FOXO1 (C29H4 ...
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bioRxiv - Immunology 2024Quote: ... CD138-BV650 (Biolegend, Clone: 281-2), CD31-APCCy7 (Biolegend ...
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bioRxiv - Immunology 2024Quote: ... and monensin (2 mM) (Biolegend, 420701) in iron free media at 37°C/5% CO2 for 5h prior to staining.
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bioRxiv - Bioengineering 2024Quote: ... α-total ERK1/2 (Biolegend 686902) 5000x ...
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bioRxiv - Immunology 2023Quote: ... IL-2-PE (JES6-5H4; Biolegend), TNFα-Fitc (MP6-XT22) ...
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bioRxiv - Immunology 2024Quote: ... Anti-CD138-APC (281-2) (Biolegend), Anti-CD83-PeCy7 (Michel-19) ...
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bioRxiv - Neuroscience 2024Quote: ... CD202b (TIE-2) (BioLegend, Cat#334204), CD146 (BioLeg-end ...
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bioRxiv - Immunology 2024Quote: ... IL-2-Alexa700 [all from BioLegend] ...
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bioRxiv - Cell Biology 2024Quote: ... anti-CLEC2 (17D9/CLEC-2, Biolegend), anti-CD117 (2B8 ...
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bioRxiv - Cancer Biology 2024Quote: ... anti-mouse H-2 (Biolegend, 125506), anti-mouse I-A/I-E (Biolegend ...
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bioRxiv - Cancer Biology 2023Quote: ... IL-2 and TNFα kits (BioLegend) and the experiments were done according to the manufacturer’s protocol ...
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bioRxiv - Immunology 2023Quote: ... 200 IU/ml IL-2 (Biolegend), 12.5% FBS ...
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bioRxiv - Immunology 2023Quote: ... anti-CD49d (Biolegend, clone: R1-2), anti-NK1.1 (Biolegend ...
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bioRxiv - Immunology 2022Quote: ... IgD BV480 (Biolegend, clone IA6-2), CD5 BV785 (BD bioscience ...
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bioRxiv - Microbiology 2023Quote: ... IL-2 (clone JES6-5H4, BioLegend), TNF- α (clone MP6-XT22 ...
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bioRxiv - Immunology 2022Quote: ... IgD PC7 (Biolegend, clone IA6-2). Flow cytometry was performed as above with a BD Fortessa Analyzer and the FlowJo software.
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bioRxiv - Microbiology 2023Quote: ... IL-2 and IL-7 (Biolegend) were added at 10 ng/ml final concentration ...