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Citations for BioLegend :
1601 - 1650 of 3960 citations for Recombinant Human CD3d Molecule Delta CD3 TCR Complex His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2024Quote: ... cells were harvested and stained with viability dyes and surface antibodies (anti-CD19, anti-CD11b, anti-CD3, and anti-CD4, BioLegend) for 30 minutes at 4°C followed by fixation with 2% paraformaldehyde ...
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bioRxiv - Immunology 2024Quote: γδ IELs were cultured ex vivo as previously described (33) in which 100,000 γδ IELs were activated with 1 ÿg/mL anti-CD3 (2C11, BioLegend) in the presence of 10 U/mL IL-2 ...
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bioRxiv - Immunology 2023Quote: ... CD4+CD62LhighCD44low/- T cells were purified from the spleen of wild-type BALB/c mice post staining with antibodies to mouse CD3 (17A2, Biolegend), CD4 (RM4-5 ...
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bioRxiv - Immunology 2023Quote: ... 100 uL/well) were restimulated with coated anti-CD3 antibody (1 ug/mL, 100 uL/well, 4 °C overnight, Clone: OKT3, Biolegend) and soluble anti-CD28 antibody (2 ug/mL ...
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bioRxiv - Cancer Biology 2023Quote: ... Transfected cells were then stimulated for 24h at 37°C with plate bound 1μg/ml of anti-CD3 (clone UCHT1, BioLegend #300402) and 2μg/ml of anti-CD28 (clone CD28.2 ...
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bioRxiv - Cancer Biology 2023Quote: ... Jurkat transfectants were activated for 5 minutes at 37°C in serum-free RPMI-1640-sodium bicarbonate by crosslinking the anti-CD3 antibody (1μg/ml, clone UCHT1, BioLegend #300402) with secondary anti-mouse antibodies for immunoblot analysis of GSK-3 α/β and Akt phosphorylation.
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bioRxiv - Cancer Biology 2023Quote: ... PBMCs and HeLa target cells were co-cultured in u-bottom 96 well plates at a range of T:E ratios (indicated in figure legends) T cells were stimulated with 10 µg/mL plate-immobilised anti-CD3 (OKT3, BioLegend) and cultured for 4 days prior to staining with anti-CD8-APC (SK1 ...
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bioRxiv - Cancer Biology 2023Quote: ... Wells of a 24-well plate were coated with 1 μg/mL anti-CD3 (#317326) and anti-CD28 antibodies (#302934, both BioLegend). For activation of T-cells ...
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bioRxiv - Immunology 2023Quote: T-IEL isolated from untreated Nr4a3-Tocky mice or mice that were injected with anti-CD3 (Clone 145-2C11) or IgG (both Biolegend) were stained with Viability dye-efluor780 (eBiosciences) ...
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bioRxiv - Immunology 2023Quote: ... Primary antibodies used were: Vimentin 1:500 (Standard BioTools, #3143027D), CD3 1:250 (Dako, #A0452, Lysozyme 1:400 (Biolegend, #860001), CD11c 1:300 (Abcam ...
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bioRxiv - Immunology 2023Quote: Staining for flow cytometry was done using the following anti-mouse mAbs: CD3 (17A2) conjugated to PE/Dazzle 594 (BioLegend), CD4 (RM4-5 ...
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bioRxiv - Immunology 2023Quote: Harvested lungs and spleen immune cells were incubated with recommended dilutions of antibodies conjugated to fluorophores: CD3-BV510 (BioLegend, #100234), CD4-BV785 (BioLegend ...
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bioRxiv - Immunology 2023Quote: ... for 5 min on ice before staining with fluorochrome-conjugated antibodies in FACS staining buffer (1× PBS, 2% FBS): CD3 (1:50 dilution; clone 145-2C11, BioLegend), CD8α (1:200 dilution ...
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bioRxiv - Immunology 2023Quote: ... electroporated Jurkat cells were interrogated by pMHC-multimer staining with the following surface panel: anti-CD3 APC Fire 50 (SK7, Biolegend), anti-CD8 Pacific Blue™ (RPA-T8 ...
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bioRxiv - Immunology 2023Quote: ... Stained T-IEL and unstained lymph node T cells were mixed at a 1:1 ratio before stimulation with 30 μg/ml anti-CD3 (Clone 145-2C11, Biolegend) crosslinked with 5 μg/ml anti-Hamster (Jackson Immunoresearch) ...
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bioRxiv - Bioengineering 2023Quote: ... CD3/CD28 Beads were magnetically removed and transduction efficacy was analyzed by staining with fluorophore-conjugated antibodies (all from Biolegend) and 7-AAD staining solution (Miltenyi Biotec ...
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bioRxiv - Bioengineering 2023Quote: ... the cells were stained for 30 min at 4°C with surface antibodies: anti-mouse CD3 (Biolegend, APC Fire 810), CD4 (Biolegend ...
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bioRxiv - Immunology 2023Quote: ... CD8+ T cells were activated in 96-wells culture plates (25 x103 cells/well) coated with anti-CD3 and anti-CD28 (Biolegend) or by the addition of the DPP-8/9 inhibitor Talabostat (Val-boroPro ...
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bioRxiv - Cancer Biology 2023Quote: ... or cells cultured in vitro were subjected for surface marker staining with the following reagents for 20 min at 4 °C: Brilliant Violet 650TM-conjugated anti-mouse CD3 Ab (clone 17A2; BioLegend), PerCP/Cyanine5.5-conjugated anti-mouse CD4 Ab (clone GK1.5 ...
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bioRxiv - Immunology 2023Quote: ... CD4+ and CD8+ T cells from PBMC were stained with the following cell surface markers in MACS buffer at room temperature for 20 mins: CD3-AF488 (clone OKT3, Biolegend), CD14-eFluor450 (clone 61D3 ...
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bioRxiv - Immunology 2024Quote: ... CD4 conv T cells were resuspended in complete RPMI 10% FBS and incubated for 1 hour at 37C with 1 ug/ml of anti-mouse CD3 (2c11 clone; Biolegend) or left untreated ...
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bioRxiv - Cancer Biology 2023Quote: PB or BM cell suspensions in PBE buffer were stained on ice for 20 min with the indicated multi-colour antibody panels consisting of fluor-labelled antibodies directed against CD3 (13-0032-82, eBioscience/100206, Biolegend), CD4 (13-0043-82 ...
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bioRxiv - Microbiology 2024Quote: ... the cells were resuspended with the CD3 T-cell antibody conjugated with Brilliant Violet 605 (Clone: 17A2; Cat. No. 100237) purchased from BioLegend at the desirable concentration (1:100 ...
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bioRxiv - Immunology 2024Quote: ... T cell stimulations were performed by first coating a 96-well plate with 5 μg/mL anti-mouse CD3 (Biolegend) in 50 μL/well PBS overnight the day before tissue harvest ...
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bioRxiv - Immunology 2024Quote: ... CTLs were seeded on sapphire discs coated with poly-L-ornithine (0.1 mg/ml) and anti-CD3 UCHT1 (30 µg/ml, BioLegend # 300402) in flat specimen carriers (Leica) ...
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bioRxiv - Immunology 2024Quote: ... which were coated with poly-L-ornithine (0.1 mg/ml) and mouse anti-CD3 UCHT1 (30 µg/ml, BioLegend # 300402), for 10 min at RT (20°C ± 2°C ...
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bioRxiv - Cancer Biology 2020Quote: ... Cell sorting for GFP-positive CD298+MHC-I− PDX cells with fluorescently tagged antibodies for CD298 (Biolegend, cat. no. 341704) and MHC-I (eBioscience ...
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bioRxiv - Cell Biology 2022Quote: ... or P14 bone marrow cells from 2-4 mice per genotype were c-kit selected and stained with tagged Total-Seq B antibodies (Biolegend) to CD150 ...
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bioRxiv - Immunology 2023Quote: ... Single cell suspensions were then tagged with mouse Total-Seq anti-mouse hashtags C0306 (Brafi: Hashtag 6) and C0307 (Brafi + C-Me: Hashtag 7) (BioLegend) according to manufacturers’ protocols.
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bioRxiv - Microbiology 2020Quote: ... incubated for 1 hour with mouse HRP α-His Tag antibody (1:1000 in 5% milk) (BioLegend, USA) and developed ...
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bioRxiv - Bioengineering 2023Quote: ... the cells were washed with PBS containing 1% FBS and incubated with APC anti-His Tag Antibody (BioLegend) for 20 minutes on ice ...
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bioRxiv - Immunology 2023Quote: ... Binding of anti-TIGIT antibodies HB0036/HB0030 to cell surface was detected by APC-anti his (Biolegend/362605). For ELISA based assay of anti-TIGIT antibodies in tumour digest ...
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bioRxiv - Immunology 2023Quote: ... with a difference that the cells were Fc blocked with both human Fc blocker (Human TruStain FcX, Biolegend) and mouse Fc blocker (TruStain FcX™ PLUS (anti-mouse CD16/32 ...
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bioRxiv - Immunology 2022Quote: ... then incubated at 37 °C in the presence or absence of recombinant murine CCL28 (BioLegend) at the indicated concentrations ...
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bioRxiv - Immunology 2021Quote: ... Sorted transduced macrophages were stimulated with 5 ng/mL recombinant murine IFN-γ (575304, Biolegend) 12-14 hours before stimulation with 10 ng/mL recombinant TNFα as described above for 4 hours (FBS used in these experiments was from Omega ...
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bioRxiv - Immunology 2024Quote: ... BMDCs were generated by cultivating BM cells supplemented with recombinant mouse GM-CSF (#576308, BioLegend) or recombinant mouse Flt3L (550706 ...
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bioRxiv - Immunology 2024Quote: WT C57Bl/6 mice were injected intraperitoneally with 250 ng recombinant mouse IL-33 (Biolegend) in 100 µl PBS for three consecutive days ...
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bioRxiv - Developmental Biology 2023Quote: ... one uterine horn of XER mice was injected intraluminally with 1µg recombinant Lif (BioLegend, 554006) at 1300h on GD3 ...
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bioRxiv - Cancer Biology 2023Quote: ... 100 ng/ml recombinant mouse (rm)-SCF (Miltenyi) and 20 ng/ml rm-TPO (BioLegend), in the presence or absence of 300 pg/ml rm-IL21 (R&D Systems) ...
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bioRxiv - Cancer Biology 2023Quote: ... 100 ng/ml recombinant mouse (rm)-SCF (Miltenyi) and 20 ng/ml rm-TPO (BioLegend), in the presence or absence of 300 pg/ml rm-IL21 (R&D Systems) ...
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bioRxiv - Cancer Biology 2021Quote: ... incubated with human Fc receptor block (BioLegend), and stained with cell surface antibodies (1:100 anti-CD137-APC [clone 4B4-1 ...
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bioRxiv - Cell Biology 2022Quote: ... and anti-human CD45-APC (Biolegend, 368512). Human cell subpopulations were detected in the bone marrow of transplanted mice using the following antibodies ...
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bioRxiv - Cell Biology 2022Quote: ... anti-human CD34-Alexa 488 (Biolegend, 343518) and anti-human CD235a-APC (Ebioscience ...
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bioRxiv - Molecular Biology 2021Quote: ... using the LEGENDplex Human Angiogenesis Panel (BioLegend) bead-based immunoassay ...
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Single-cell analysis of skeletal muscle macrophages reveals age-associated functional subpopulationsbioRxiv - Cell Biology 2022Quote: ... PE anti-mouse/human CD11b Antibody (Biolegend, Cat# 101208 ...
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bioRxiv - Immunology 2021Quote: Cells were incubated with Human FcX (Biolegend) to prevent non-specific antibody binding ...
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bioRxiv - Immunology 2019Quote: ... and mouse anti-human CD45 (HI30, Biolegend) overnight followed by 1hr incubation at room temperature with goat anti-mouse Cy3 (Jackson laboratory ...
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bioRxiv - Synthetic Biology 2019Quote: ... and mouse anti human CD4 APC (Biolegend),
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bioRxiv - Cancer Biology 2019Quote: ... and APC anti-human CD4 (Biolegend 300514). 68% of sgCD45-transduced cells expressed CD45 and 28% of sgCD4-transduced cells expressed CD4.
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bioRxiv - Immunology 2019Quote: ... blocked with human TruStain FcX block (Biolegend) for 10 minutes at room temperature with shaking ...