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Citations for BioLegend :
1551 - 1600 of 3960 citations for Recombinant Human CD3d Molecule Delta CD3 TCR Complex His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2021Quote: 96-well round-bottom plates were coated with either 2.5 µg/mL anti-CD3 (Biolegend, clone 145-2C11,Cat# 100359) or HIgG (Biolegend ...
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bioRxiv - Cancer Biology 2021Quote: ... Cells were then stained in FC buffer (2% FCS in PBS) with anti-CD3-APC conjugated antibodies (1:80; BioLegend) and LIVE/DEAD Fixable Near-IR Dead Cell Stain (1:1000 ...
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bioRxiv - Immunology 2021Quote: ... 1:400), CD4-Spark685 (SK3, 1:200), streptavidin-APC-Fire750, CD3-APC-Fire810 (SK7, 1:50) and Zombie NIR (all BioLegend) diluted in Brilliant Staining buffer (BD Horizon) ...
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bioRxiv - Immunology 2021Quote: ... 1:400), CD4-Spark685 (SK3, 1:200), streptavidin-APC-Fire750, CD3-APC-Fire810 (SK7, 1:50) and Zombie NIR (all BioLegend) diluted in Brilliant Staining buffer (BD Horizon) ...
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bioRxiv - Immunology 2022Quote: ... Total CD3+ T cells or naïve CD4+ T cells were enriched from mouse spleen and lymph nodes by negative selection using MojoSort Mouse CD3 T Cell Isolation Kit or MojoSort Mouse CD4 Naive T Cell Isolation Kit (MojoSort, Biolegend) following the manufacturer’s instructions ...
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bioRxiv - Genomics 2020Quote: ... The remaining 450μl of cell suspension was stained with 15 μl of each of the following antibodies: CD3 APC (Biolegend, 300411), CD4+ FITC (BD ...
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bioRxiv - Microbiology 2019Quote: ... and fluorescence-labelled monoclonal antibodies against CD3 (FITC, eBioscience) and CD4 (Alexa Fluor700 or Brilliant Violet 605, both from BioLegend). After fixation and permeabilization ...
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bioRxiv - Immunology 2019Quote: ... Cells were fixed and permeabilized with Foxp3 / Transcription Factor Staining Buffer Set (eBiosciene) and afterwards intracellularly stained with CD3-AlexaFluor700 and Ki-67-BV711 (clone Ki-67, Biolegend). In-depth analysis of CD27−IgD− B cell phenotype was performed with Fixable viability stain 780 or aqua viability dye V510 ...
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bioRxiv - Immunology 2021Quote: CD3+ T cells from lung single-cell suspensions (1×107 cells) were isolated by negative selection using the MojoSort mouse CD3+ T-cell Isolation kit (Biolegend) according to the manufacturer’s instructions ...
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bioRxiv - Bioengineering 2020Quote: The following antibodies were used for phenotyping and proliferation assays: anti-CD3-PE/Cy7 (clone SK7, Biolegend, San Diego, CA), anti-CD4-PerCP (clone SK3 ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... The suspended cells were then transferred to a fresh flask and activated in fresh RPMI-1640/10% FBS/PSG by 3:20,000 anti-CD3 (BioLegend 317303), 3:20,000 anti-CD28 (BioLegend 302913) ...
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bioRxiv - Immunology 2021Quote: ... 0.05 mM β-mercaptoethanol) on a 96-well flat bottom plate coated with anti-CD3 antibody (10µg/ml, cat. # 317325, BioLegend). 7 x 103 DC3 or 5 x 104 monocytes were used per well ...
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bioRxiv - Cancer Biology 2020Quote: ... Cells were seeded at a density of 2.5 × 104/well in 96-well plates pre-coated with 1ug/ml CD3 (Biolegend100339) and 1ug/ml CD28 (Biolegend 102115) antibodies and cultured in 1640 media with 10% FBS ...
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bioRxiv - Cell Biology 2021Quote: ... Unfractionated or c-Kit-enriched BM cells were then incubated with purified rat anti-mouse lineage antibodies (CD3, BioLegend, 100202; CD4, eBioscience, 16-0041-82; CD5, BioLegend, 100602; CD8, BioLegend, 100702; CD11b, BioLegend, 101202; B220, BioLegend, 103202 ...
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bioRxiv - Cell Biology 2021Quote: ... Unfractionated or c-Kit-enriched BM cells were then incubated with purified rat anti-mouse lineage antibodies (CD3, BioLegend, 100202; CD4, eBioscience, 16-0041-82; CD5, BioLegend, 100602; CD8, BioLegend, 100702; CD11b, BioLegend, 101202 ...
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bioRxiv - Cancer Biology 2022Quote: ... the phenotype of live T-/CAR T-lymphocytes in all the experimental conditions was assessed using the following antibodies: BV510-anti CD3 (clone OKT3, BioLegend), APC-CY7-anti CD3 (clone UCHT1 ...
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bioRxiv - Cell Biology 2022Quote: ... isolated neutrophils were labeled with a panel of primary antibodies (anti-CD3, anti-CD45, anti-CD19, anti-CD14, anti-CD66b, anti-CD15 from Biolegend) for 20 minutes at room temperature and washed twice ...
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bioRxiv - Cancer Biology 2022Quote: ... for 24h at 4°C on a shaker and then stained with antibodies (rat anti-CD3 [clone 17A2, cat#100202, Biolegend] ...
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bioRxiv - Immunology 2022Quote: ... we obtained splenocytes from WT and TCF-7 cKO mice and either activated them with 2.5ug/ml anti-CD3 (Biolegend #100202) and anti-CD28 antibodies (Biolegend #102115 ...
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bioRxiv - Immunology 2022Quote: ... we obtained splenocytes from WT and TCF-7 cKO mice and stimulated T cells with 2.5ug/ml anti-CD3 (Biolegend #100202) and anti-CD28 antibodies (Biolegend #102115 ...
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bioRxiv - Immunology 2022Quote: ... cells were transferred to a non-CD3 coated plate and incubated for an additional three days before activation with Cell Activation cocktail with Brefeldin A (Biolegend) for 6 h and collection ...
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bioRxiv - Immunology 2021Quote: Total CD3+ T cells or naïve CD4+ T cells were enriched from mouse spleen and lymph nodes by negative selection using MojoSort Mouse CD3 T Cell Isolation Kit or MojoSort Mouse CD4 Naive T Cell Isolation Kit (MojoSort, Biolegend) following the manufacturer’s instructions ...
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bioRxiv - Immunology 2022Quote: ... The cells were then incubated for 20 min in the dark with the following fluorophore-conjugated antibodies CD3 (17A2; Biolegend), CD4 (GK1.5 ...
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bioRxiv - Immunology 2019Quote: ... glass slides or 8 well chambers were coated with either ICAM-1 alone or anti-CD3 (clone Hit3a from Biolegend)/anti-CD28 (clone CD28.2 from Biolegend)/ICAM-1 (R&D systems ...
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bioRxiv - Immunology 2019Quote: ... The following antibodies were used for extracellular and intracellular staining as 2 different panels: anti-CD3 Alexa700 (clone UCHT1; BioLegend), anti-CD3 PerCp Cy5.5 (clone UCHT1 ...
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bioRxiv - Immunology 2020Quote: ... CD4+ T cell purity was assessed by flow cytometry using anti-CD4 Alexa 700 (eBioscience) and anti-CD3 Brilliant Violet (BV) 785 (BioLegend) antibody staining ...
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bioRxiv - Immunology 2019Quote: ... Pre-enrichment for antigen presenting cells prior to sorting was done with biotinylated anti-CD3 (clone 145-2C11) and anti-CD19 (clone 6D5) both from Biolegend. Cell suspensions were incubated with antibodies for 10 minutes at room temperature at a dilution of 1:200 ...
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bioRxiv - Cell Biology 2021Quote: ... clone HIT2), Lineage marker cocktail (BV510, CD3/14/16/19/20//56, clone OKT3/M5E2/3G8/HIB19/2H7/HCD56) (BioLegend) and PLXDC2 (PE/APC ...
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bioRxiv - Cell Biology 2021Quote: ... Unfractionated or c-Kit-enriched BM cells were then incubated with purified rat anti-mouse lineage antibodies (CD3, BioLegend, 100202; CD4, eBioscience, 16-0041-82; CD5, BioLegend, 100602; CD8, BioLegend, 100702 ...
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bioRxiv - Cell Biology 2021Quote: ... Unfractionated or c-Kit-enriched BM cells were then incubated with purified rat anti-mouse lineage antibodies (CD3, BioLegend, 100202; CD4, eBioscience, 16-0041-82; CD5, BioLegend, 100602 ...
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bioRxiv - Immunology 2020Quote: ... Cells were stained with the tetramer plus CD3-eFluor 450 (1/100, 48-0032-82 eBioscience) and CD8-FITC (1/100, 300905 BioLegend) at 4°C for 30 min ...
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bioRxiv - Immunology 2021Quote: ... sort-purified γδ or αβ IELs were seeded at approximately 400K cells per well in round-bottom 96-well plates coated overnight with 2 μg/ml anti-CD3 (145-2C11, BioLegend), 2 μg/ml polyclonal Armenian hamster IgG (BioXCell) ...
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bioRxiv - Immunology 2020Quote: ... Anti-CD69 (H1.2F3, PE/Cy7, B253212, 104512), Anti-CD3 (17A2, APC/Cyanine7, B283940, 100222) antibodies were purchased from BioLegend (Bxxxxxx) or BD Biosciences (xxxxxxx).
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bioRxiv - Immunology 2020Quote: ... The pellet was resuspended in flow cytometry staining buffer (FACS) buffer containing 1:100 dilution of CD3 antibody (Cat≠317307; Biolegend) and incubated for 30 minutes at 4°C in the dark ...
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bioRxiv - Immunology 2020Quote: ... total thymocytes were activated with 1 μg/mL plate bound anti-CD3 and soluble 1 μg/mL anti-CD28 in the presence of monensin (BioLegend). After 4hrs ...
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bioRxiv - Immunology 2020Quote: ... Slides were incubated with monoclonal antibodies overnight at 4°C using anti-CD3 (100202, clone 17A2, BioLegend, San Diego, CA) to detect T-cells ...
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bioRxiv - Immunology 2020Quote: ... lymph nodes were removed from Myc-eGFP mice and mashed and cell suspension activated with anti-CD3 (1 µg/mL, clone 145-2C11, Biolegend) and anti-CD28 (3 µg/mL ...
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bioRxiv - Immunology 2020Quote: ... Cells were then stained with anti-CD3-FITC (clone 145-2C11; 1:100; eBioscience) and anti-TCRδ (clone GL3; 1:100; Biolegend) for 30 min on ice ...
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bioRxiv - Immunology 2022Quote: ... CD4+ T cells or CD3+ T cells were sorted into CXCR4hi T cells and CXCR4low T with anti-CXCR4 (Cat # 306510, Biolegend) on FACS Aria-III (Becton Dickinson) ...
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bioRxiv - Cancer Biology 2022Quote: ... After cell counting 1×106 cells were stained with a cocktail for lineage markers (CD3, CD11b, B220, Ter119, and Ly6G/6C; BioLegend), and lineage-negative cells (Lin- ...
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bioRxiv - Cancer Biology 2022Quote: ... and activated using plate-bound anti-CD3 (1μg/ml; clone-145-2C11) and anti-CD28 (5μg/ml; clone-37.51) antibodies (Biolegend; San Diego, CA) for 4 days.
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bioRxiv - Genomics 2022Quote: ... flat bottom 96-well plates were coated with 2 μg/mL of anti-CD3 (Clone: 145-2C11, BioLegend, Cat#100302) in PBS overnight at 4°C or 4 hrs at 37°C ...
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bioRxiv - Immunology 2022Quote: ... prior to incubation with staining antibodies (PE/Cy7 anti-mouse CD45, BV711 anti-mouse CD3, BV421 anti-mouse CD19, FITC anti-mouse CD31, and APC anti-mouse podoplanin, all BioLegend) and one Hashtag antibody per replicate using BioLegend TotalSeq-B03 (catalog numbers 155831 ...
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bioRxiv - Immunology 2022Quote: ... CD4+ T-cells were cultured alone or co-cultured with either of the B-cell populations at a ratio of 3:1 for 60 h at 37°C and 5% CO2 on anti-CD3 (2 μg/mL) (ULTRA-LEAF Biolegend) coated flat bottomed 384 CellCarrier Ultra microplates (PerkinElmer ...
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bioRxiv - Immunology 2022Quote: ... and then stained with the appropriate extracellular antibodies (anti-CD45, clone H130, Biolegend; anti-CD3, clone HIT3a, Biolegend; anti-CD4, clone RPA-T4, Biolegend; anti-CD45RA, clone HI100, Biolegend; anti-CD45RO ...
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bioRxiv - Immunology 2022Quote: ... and then stained with the appropriate extracellular antibodies (anti-CD45, clone H130, Biolegend; anti-CD3, clone HIT3a, Biolegend; anti-CD4, clone RPA-T4, Biolegend; anti-CD45RA ...
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bioRxiv - Immunology 2022Quote: ... Samples were then stained for extracellular markers in the presence of Fc block for 30 minutes at 4°C protected from light using the following antibodies: CD3 (APC/Cy7; 1:300; clone 17A2; BioLegend), CD4 (AF488 ...
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bioRxiv - Cancer Biology 2023Quote: PB or BM cell suspensions in PBE buffer were stained on ice for 20 min with the indicated multi-colour antibody panels consisting of fluor-labelled antibodies directed against CD3 (13-0032-82, eBioscience/100206, Biolegend), CD4 (13-0043-82 ...
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bioRxiv - Immunology 2024Quote: ... CD4 conv T cells were resuspended in complete RPMI 10% FBS and incubated for 1 hour at 37C with 1 ug/ml of anti-mouse CD3 (2c11 clone; Biolegend) or left untreated ...
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bioRxiv - Immunology 2023Quote: ... CD4+ and CD8+ T cells from PBMC were stained with the following cell surface markers in MACS buffer at room temperature for 20 mins: CD3-AF488 (clone OKT3, Biolegend), CD14-eFluor450 (clone 61D3 ...