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Citations for BioLegend :
1851 - 1900 of 3960 citations for Recombinant Human CD3d Molecule Delta CD3 TCR Complex His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2024Quote: Bone marrow derived-DCs (BMDCs) were prepared using 10 ng/mL recombinant GM-CSF (Biolegend, #576306), as previously described [18] ...
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bioRxiv - Immunology 2024Quote: The cytokine stimulation conditions were performed with commercially available recombinant IL-12 (100 ng/mL; BioLegend), recombinant IL-18 (100 ng/mL ...
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bioRxiv - Neuroscience 2020Quote: For analysis of LILRB2 expression on marmoset and human monocyte derived macrophages were first blocked with human TruStain FcX Fc blocking solution (Biolegend), then labelled with and without anti-human LILRB2 (5 µg/mL ...
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bioRxiv - Bioengineering 2021Quote: ... Naïve T cell marker staining was performed with APC/Cy7 mouse anti-human CD45RA (#304128) and BV510 mouse anti-human CD45RO (#304232) antibodies from Biolegend.
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bioRxiv - Immunology 2021Quote: ... Tonsillar IgD+ B cells were isolated from human tonsil cells by positive selection using biotin-anti-human IgD mAb (clone IA6-2; 348212, Biolegend) and MagniSort™ Streptavidin Positive Selection Beads (MSPB-6003-74 ...
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bioRxiv - Immunology 2022Quote: ... PBMCs were surface stained with series of antibodies (anti-human CD158 [KIR2DL1/S1/S3/S5]-FITC [BioLegend, Cat#339504], anti-human CD158b [KIR2DL2/L3, NKAT2]-FITC [BioLegend, Cat#312604] ...
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bioRxiv - Cancer Biology 2022Quote: ... Antibodies used for staining of human tumor cells included: anti-human EpCAM-FITC (BioLegend 324204, clone 9C4, dilution 1:30), anti-human/mouse CD49f-APC (BioLegend 313616 ...
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bioRxiv - Biophysics 2022Quote: ... Monoclonal antibodies against human Glycophorin AB (CD235ab) (HIR2; catalog number: 306602) and human CD47 (CC2C6; catalog number: 323102) were purchased from BioLegend.
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bioRxiv - Immunology 2019Quote: ... Supernatants were analyzed by using either the Legendplex Multi-Analyte Flow Assay Kit for human CD8/NK cells or the human Th cytokine panel (Biolegend).
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bioRxiv - Immunology 2021Quote: ... containing anti-CD9 (human HI9a, mouse MZ3), anti-CD63 (human H5C6, mouse NVG-2), anti-CD81 antibodies (human 5a6, mouse Eat-2) from Biolegend, and anti-human ACE2 (R&D Biosystems cat no ...
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bioRxiv - Microbiology 2021Quote: ... Mouse IgG and human IgG were detected with separate secondary antibodies (goat anti-human IgG Fc, goat anti-mouse IgG Fc, BioLegend) at a dilution of 1:4,000 in blocking buffer and PNPP as described above.
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bioRxiv - Immunology 2020Quote: ... For human cytokine IL-4 in the supernatant of human ILC2 cell cultures were measured by ELISA kit from BioLegend. All final reactions were developed with TMB substrate (Thermo scientific ...
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bioRxiv - Immunology 2020Quote: ... human Interlukin-6 (IL-6) (100 ng/ml) and human IL-6Rα (100 ng/ml) (BioLegend, San Diego, CA, USA).
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bioRxiv - Cell Biology 2022Quote: ... Cells were incubated with antibodies directly conjugated to fluorescent dyes (anti-human BAFF-R conjugated to FITC, BioLegend, Cat. #: 316904; anti-human BCMA conjugated to APC, BioLegend, Cat ...
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bioRxiv - Immunology 2022Quote: The untouched resting B cells were isolated from the human tonsils by depletion of other cells using MojoSort Human B Cell (CD43-) Isolation Kit (BioLegend), whereas the naïve B cells were isolated using the EasySep Human Naïve B cell isolation kit (Stem Cell Technologies) ...
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bioRxiv - Microbiology 2022Quote: ... using 1:1000 of polyclonal rabbit anti-human MCT1 IgG (Cell Signalling) and 1:1000 mouse anti-human CD147 IgG (Biolegend) followed by HRP-conjugated secondary IgG antibody of specific host type ...
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bioRxiv - Microbiology 2024Quote: CD14+ monocytes were isolated from PMBCs of healthy human blood using the MojosortTM Human CD14 Selection Kit (BioLegend, Cat# 480026) To generate monocyte derived dendritic cells (moDCs) ...
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bioRxiv - Immunology 2023Quote: ... FITC anti-human CD18 antibody and FITC anti-human CD11c antibody (clone Bul15) were purchased from BioLegend (San Diego, USA). Rabbit anti-human MERTK antibody (D21F11 ...
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bioRxiv - Immunology 2023Quote: ... THP-1 and SKOV-3 cell lines and primary human T cells were labeled with fluorescent antibody against human CD70 or isotype control (Biolegend) to verify antigen expression as described before35.
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bioRxiv - Immunology 2024Quote: ... IL-2 (anti-human IL-2, PE-Cy7, clone MQ1-17H12), and CD154 (anti-human CD154, APC, clone 24-31) all from Biolegend in Perm wash buffer (PBS + 0.02% NaN3 + 0.5% bovine serum albumin (BSA ...
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bioRxiv - Genomics 2020Quote: ... Expression of cell surface markers in resting and CD3/CD28 activated cells was made by flow cytometry using fluorescent-coupled antibodies against CD4 (Biolegend, Cat: 100434), CD3 (Biolegend ...
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bioRxiv - Microbiology 2021Quote: ... Intracellular P-STAT5 staining was then performed in PBS with wash steps performed at 1800 rpm for 6 min at 4°C.The following antibody clones and fluorochromes were used: CD3 (UCHT1, Biolegend; Bv510, Bv711, FITC), CD8 (SK1 ...
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bioRxiv - Immunology 2022Quote: ... Cells were then stained at room temperature for 45 minutes with the following antibodies: CD3 BV650 (OKT3; Biolegend, San Diego, CA, USA), CD4 BV785 (OKT4 ...
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bioRxiv - Immunology 2022Quote: ... Cells were then stained at room temperature for 45 min with the following antibodies: CD3 BV650 (OKT3; BioLegend, San Diego, CA, USA), CD4 BV785 (OKT4 ...
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bioRxiv - Immunology 2020Quote: ... Cells were then permeabilised with Saponin 2% in PBS for 20 min at room temperature and then stained in Saponin 2% in PBS with the appropriate antibodies: Th-1 (anti-CD3-BV510 (1:100, Biolegend, Cat#300448), anti-CD4-PE (1:100 ...
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bioRxiv - Physiology 2019Quote: ... overnight at a 1:1 ratio with the indicated target melanoma cells or activated with 1μg/ml plate-bound anti - CD3 (Biolegend, clone: 145-2C11) as indicated in each experiment ...
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bioRxiv - Physiology 2019Quote: ... were co-cultured for 6h at 37°C at a 1:1 ratio with the indicated target melanoma cells or activated with 1μg/ml anti-CD3 (Biolegend, clone: 145-2C11). After 2 h ...
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bioRxiv - Biophysics 2020Quote: ... The lineage depleted fraction was stained with 13 markers for lineage-specfic monoclonal antibodies FITC (CD2, CD3, CD4, CD11b, CD14, CD15, CD24, CD10, CD41, CD20, CD66c, CD127, CD33) (Biolegend and BD) and Pacific blue conjugated anti-CD34 (Biolegend) ...
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bioRxiv - Immunology 2021Quote: ... plates were coated at least 5 hours prior to use with 3 ug/ml plate-bound anti-CD3 antibody (clone OKT3; BioLegend, LEAF grade), 1 ug/ml soluble anti-CD28 antibody (BioLegend ...
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bioRxiv - Immunology 2021Quote: ... plates were coated at least 5 hours prior to use with 5 ug/ml plate-bound anti-CD3 antibody (clone OKT3; BioLegend, LEAF grade), which is then washed off with PBS ...
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bioRxiv - Cancer Biology 2022Quote: ... some samples were additionally stained with antibodies to enrich for T cell populations (NB124, NB125, NB138 with CD3-PE (Biolegend, cat#300308); NB124 with TCRγδ-BV421 (Biolegend ...
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bioRxiv - Cell Biology 2024Quote: S6K phosphorylation was measured by intracellular staining in murine CD8+ T cells activated for 24 h with plate-bound anti-CD3 (coated at 5 µg / ml for 14 h at 4 °C in PBS; BioLegend, Cat. # 100331) and 1 µg / ml soluble anti-CD28 (BioLegend ...
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bioRxiv - Immunology 2024Quote: ... and then surface labelled for 30 min at 4°C with TotalSeq-C0182 anti-mouse CD3 (clone 17A2, dilution 1:50, Biolegend, cat # 100263) and TotalSeq-C0002 anti-mouse CD8α (clone 53-6.7 ...
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bioRxiv - Immunology 2024Quote: ... CD19-expressing K562 (CAR Activation) or CD64- and CD80-expressing K562 previously loaded with anti-CD3 antibody (OKT3, Biolegend, San Diego, CA) at 1 μg/ml for 1 hour 39 (TCR/CD28 Activation ...
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bioRxiv - Immunology 2024Quote: ... Primary lymphocytes isolated from skin draining lymph nodes from adult C57BL/6 mice were cultured on plates precoated with anti-mouse α-CD3 (3 μg/ml, Biolegend, #100340) and anti-mouse α-CD28 (3 μg/ml ...
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bioRxiv - Bioengineering 2023Quote: ... T-cells that have been incubating with conditioned media were carefully transferred to the CD3-coated plates and further stimulated with 1 µg/mL anti-CD28 (BioLegend, clone: CD28.2) for another 48 hours ...
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bioRxiv - Bioengineering 2023Quote: ... the CNP labeled cells were spin washed and incubated with 10μgꞏml−1 of AF488 conjugated anti-CD3 antibodies (Biolegend, Cat # 300415, clone UCHT1) in 2% BSA/PBS on ice for 30min and then spin washed ...
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bioRxiv - Immunology 2024Quote: ... cells were first stained with AViD Live/Dead Fixable Yellow Dead Cell Staining Reagent and subsequently stained with PE anti-CD3 (Biolegend, clone UCHT1), PE/Cy7 anti-CD56 (Biolegend ...
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bioRxiv - Immunology 2024Quote: ... and PBS-57/CD1d-tetramers (NIH Tetramer Facility) for 20 mins and subsequently stained with the surface antibodies against CD3 (Biolegend cat #: 300324), CD4 (BD cat #:611887) ...
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bioRxiv - Molecular Biology 2024Quote: ... the antibody (CD8 clone RPA-T8; Biolegend Cat: 301002; CD4 clone RPA-T4; Biolegend Cat: 300502; CD3 clone UCHT1; Biolegend Cat: 300402) is incubated with DBCO-Peg5-NHS Ester (linker ...
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bioRxiv - Microbiology 2020Quote: ... stained with anti-human CD14 (Clone 63D3; Biolegend) and quantified using Attune NxT (Life Technologies) ...
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bioRxiv - Synthetic Biology 2021Quote: ... 200 ng mL-1 anti-human RP105 (Biolegend), 500 U mL-1 IL-2 (UCSF Pharmacy) ...
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bioRxiv - Cell Biology 2020Quote: ... BV421 anti-human CD14 (325627, clone HCD14, BioLegend), PE-Cy7 anti-human CD123 (560826 ...
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bioRxiv - Cell Biology 2020Quote: ... FITC anti-human Fas/CD95 (Clone DX2, Biolegend), APC anti-human Nectin-2/CD112 (Clone TX31 ...
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bioRxiv - Developmental Biology 2021Quote: ... anti-human CD148 (Biolegend, clone A3; 1:100); HDE-1 (Keller’s lab ...
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bioRxiv - Developmental Biology 2021Quote: ... anti-human CD13 (Biolegend; clone WM15; 1:200), anti-human CD90 (Biolegend ...
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bioRxiv - Developmental Biology 2020Quote: ... anti-human CD13 (Biolegend; clone WM15; 1:1,000), anti-human CD90 (Biolegend ...
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bioRxiv - Developmental Biology 2020Quote: ... anti-human CD90 (Biolegend, clone 5E10; 1:200); anti-human CD140a (PDGFRα ...
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bioRxiv - Microbiology 2022Quote: ... Purified anti-human CD81 (TAPA-1) 5A6 (Biolegend). Secondary antibodies used were Alexa 647 goat anti-mouse (Thermo Fisher Scientific ...
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bioRxiv - Immunology 2022Quote: ... and the MojoSort Human CD8 T cell (BioLegend) isolation kit ...