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Citations for BioLegend :
1551 - 1600 of 7971 citations for 5 Pyrimidinecarbonitrile 1 2R 2 3 dihydroxypropyl 1 2 3 4 tetrahydro 3 methyl 2 4 dioxo 9CI since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genomics 2022Quote: ... 0.2×106 naïve CD4+ T cells were cultured in presence of 10 ng/mL of recombinant IL-12 (BioLegend, Cat#577002), 1 ng/mL of recombinant IL-2 (BioLegend ...
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bioRxiv - Immunology 2022Quote: ... and of soluble triggering receptor expressed on myeloid cells 2 (sTREM2) and brain-derived neurotrophic factor (BDNF) were assessed using the Human LEGENDplex Multiplex Assay (BioLegend®) was used following manufacturer’s instructions (Lehmann et al ...
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bioRxiv - Immunology 2022Quote: ... Sixteen micron sections were cut and blocked in .01% Triton X 100 with 2% of FBS and donkey serum including TrueStain Monocyte Blocker (BioLegend 426101) for 1h and then stained overnight with the following directly conjugated mAbs ...
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bioRxiv - Immunology 2023Quote: ... Skin immune cells were subsequently added to each fibroblasts-placed well and co-cultured in the presence of 20 ng/ml human IL-2 recombinant and 20 ng/ml human IL-15 recombinant (BioLegend, 570304) in 24 well plates (Ratio of 50:1 immune cell-to-fibroblast) ...
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bioRxiv - Immunology 2023Quote: ... Cells were pelleted at 500xg for 5 minutes and then resuspended in 50uL of PBS+ 2% HI-FBS + 2mM EDTA in the presence of Fc receptor blocking antibody (BioLegend, 101302). Cells were labelled with antibodies as described below ...
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bioRxiv - Immunology 2023Quote: ... Cells were stained with Zombie-violet or Zombie-NIR in PBS at 1:2000 dilution for 5 minutes at room temperature prior to resuspending in PBS + 2% HI-FBS + 2mM EDTA in the presence of Fc receptor blocking antibody (BioLegend, 101302) for blocking ...
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bioRxiv - Immunology 2023Quote: Levels of CXCL9 and CXCL10 were measured in plasma (diluted 2-fold) using the mouse cytokine release syndrome panel LEGENDplex system (BioLegend, UK), according to manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2023Quote: ... and IL-2 were investigated with an enzyme-linked immunosorbent assay (ELISA MAXTM Deluxe Set, Biolegend®, San Diego, CA, USA) according to the manufacturer’s instruction ...
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bioRxiv - Bioengineering 2023Quote: ... Nanovials were reconstituted at a ten-fold dilution in Washing Buffer containing 2 μL of 100 μg/mL anti-HLA-A2 FITC antibody (Biolegend, 343304) and incubated for 30 minutes at 37°C ...
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bioRxiv - Microbiology 2023Quote: ... opsonized bacteria were re-suspended in sterile PBS with 2% fetal bovine serum and stained with APC-conjugated anti-human C3b (Biolegend 846106) and FITC-conjugated anti-human C5b-C9 (LSBio C210266 ...
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bioRxiv - Neuroscience 2023Quote: Endothelial cells isolated from brain from SARS-CoV-2 infected mice were stained with fixable viability dye (Zombie Green, Biolegend 423111) and blocked with Fc receptor blockade (anti-mouse CD16/32 ...
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bioRxiv - Bioengineering 2024Quote: ... supernatant was removed for analysis with ELISA MAX Deluxe Set Human IL-2 and ELISA MAX Deluxe Set Human IFN-γ (BioLegend). Cytotoxicity at the various indicated timepoints was tested using a luciferase-based assay ...
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bioRxiv - Immunology 2024Quote: ... the harvested cells were restimulated using a pool of overlapping Wuhan Spike peptides (Miltenyi, 130-126-701) at 2 µg/mL for 16 hours in the presence of brefeldin A (Biolegend, #400602) at 5 µg/mL ...
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bioRxiv - Immunology 2022Quote: ... CD4 (RM4-5; BV 711) and PD-1 (J43; FITC; all BioLegend). After fixation and permeabilization (Foxp3 Transcription Factor Staining Buffer Set ...
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bioRxiv - Immunology 2023Quote: ... wells were pre-coated with 5 μg/mL ICAM-1 (Biolegend, #553006) for 2-3 hours at 37°C before being emptied for seeding ...
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bioRxiv - Microbiology 2023Quote: ... CD4 (mouse, FITC fluorochrome, clone RM4-5, BioLegend 100510, 1:200 dilution), CD8a (mouse ...
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bioRxiv - Cell Biology 2022Quote: ... anti-CD64-PE/Dazzle594 (1:50, clone X54-5/7.1, Biolegend, 139320), anti-CD11b-PE (1:100 ...
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bioRxiv - Neuroscience 2024Quote: ... CD64-PE-594 (BioLegend, clone X54-5/7.1, cat #139319, 1:400), Ly6c-BV711 (BioLegend ...
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bioRxiv - Microbiology 2024Quote: ... keratin 5 (1:2000, KRT5 for basal cells; BioLegend, Catalog number: 905503), SCGB1A1 (1:200 ...
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bioRxiv - Immunology 2021Quote: ... Cells were washed with 2% FBS PBS before incubating with a panel of previously titrated 14 barcoded oligo-conjugated antibodies (BioLegend TotalSeq-C), including FITC-anti-CD45 ...
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bioRxiv - Microbiology 2020Quote: ... approximately 2-10 million total human lung cells were resuspended in MACS buffer and blocked with Human TruStain FcX (Biolegend cat# 422032) for 15min at 4°C followed by HTII-280 antibody (Terrace Biotech ...
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bioRxiv - Molecular Biology 2022Quote: ... and incubated 20-60 minutes at room temperature and continued by adding 2 mL of 1X Permeabilization Buffer (Biolegend Cat. No. 421002) and centrifuged at 400-600 x g for 5 minutes at room temperature ...
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bioRxiv - Immunology 2019Quote: ... according to the manufacturer’s instructions and cultured in the above medium supplemented with 2 μg/mL anti-human RP105 antibody clone MHR73-11 (BioLegend Cat.# 312907).
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bioRxiv - Molecular Biology 2021Quote: Enzyme-linked immunosorbent assays were completed using and according to the instructions found with the ELISA MAX™ Deluxe Set Human IL-2 (BioLegend #431804).
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bioRxiv - Immunology 2021Quote: ... prior to staining with the mAbs against cytokines (PE anti-IFN-γ, AF488 anti-TNF-α and PE-Cy7 anti-IL-2) (Biolegend) with Perm/Wash buffer (BD Biosciences) ...
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bioRxiv - Immunology 2021Quote: ... PE-conjugated anti-mouse TNF-α (clone MP6-XT22) and Pe-Cy7–conjugated anti-mouse IL-2 (clone JES6-5H4) antibodies (BioLegend, UK) for 20 min at 4°C ...
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bioRxiv - Bioengineering 2022Quote: ... Cells were stained for 30min in flow buffer (PBS, 1mM EDTA, 2% FBS) with antibodies against CD8α-FITC (53-6.7; Biolegend unless otherwise noted), CD19-PerCP/Cy5.5 (1D3) ...
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bioRxiv - Microbiology 2022Quote: Cytokine/chemokine concentrations in supernatant from mock and SARS- CoV-2 infected lung AT2 and cardiac cells were analyzed using the LEGENDplex human anti-virus response panel (Biolegend, Cat. 740390) following the manufacturer’s instructions ...
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bioRxiv - Immunology 2022Quote: ... and anti-CD45.2 APC antibodies together with TotalSeq-C anti-mouse hashtag antibodies (anti-CD45 clone 30-F11, anti-H-2 clone M1/42, Biolegend, #155869 (MH5), #155871 (MH6) ...
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bioRxiv - Bioengineering 2023Quote: ... the serum samples collected at 6 hours and 48 hours after administration were diluted 2-fold for ELISA analysis using the LEGEND MAX™ Rat IL-6 ELISA Kit with pre-coated plate (Biolegend, USA) according to the manufacturer’s recommendations with a little modification as described below ...
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bioRxiv - Bioengineering 2024Quote: ... The levels of cytokines IL-2 and IFN-γ were quantified using ELISA MAX Deluxe Set Human IL-2 and ELISA MAX Deluxe Set Human IFN-γ (BioLegend).
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bioRxiv - Immunology 2021Quote: ... fixed with 4% paraformaldehyde fixation buffer (BioLegend 420801, San Diego, CA, USA) for 20 minutes at room temperature and washed twice more with MACS buffer ...
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bioRxiv - Cell Biology 2021Quote: ... Membranes were then incubated at 4°C overnight with ATF4 antibody (BioLegend) at 1:1000 ...
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bioRxiv - Immunology 2022Quote: ... for surface staining and BV605-anti-IL-4 (504125, BioLegend Europe BV), APC-anti-IL-5 (504306 ...
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bioRxiv - Immunology 2022Quote: ... cells were fixed for 20 min with 4% paraformaldehyde (Fixation buffer; BioLegend). When intracellular staining was performed ...
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bioRxiv - Neuroscience 2021Quote: ... and CD284 (TLR-4, clone SA15-21, PE, 2.5 µg/ml Biolegend). After staining ...
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Sterilizing immunity against SARS-CoV-2 in hamsters conferred by a novel recombinant subunit vaccinebioRxiv - Microbiology 2020Quote: ... and further stained with BV421-conjugated anti-mouse IL-4 (Biolegend, 504127) and ...
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bioRxiv - Immunology 2020Quote: ... Cells were stained at 4 °C with Zombie Violet staining (BioLegend, 423113) to exclude dead cells ...
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bioRxiv - Microbiology 2021Quote: ... cells were fixed with 4% paraformaldhyde and permeabilised with CytoPerm buffer (Biolegend). Cells were analysed on BD LSR Fortessa X-20 cytometer ...
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bioRxiv - Cancer Biology 2020Quote: ... rotating at 4°C with either μ5g IRF3 antibody (BioLegend, clone:12A4A35) or equivalent isotype control conjugated with protein A/G magnetic beads ...
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bioRxiv - Immunology 2023Quote: ... and 10 µg/mL anti-IL-4 (BioLegend, Cat no. 504-102) to achieve Th1 polarization ...
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bioRxiv - Cancer Biology 2022Quote: ... and stained (20 min, 4°C) with B220-PerCP/Cyanine5.5 (#103235; BioLegend) and CD138-PE (#142504 ...
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bioRxiv - Cancer Biology 2023Quote: ... PerCP/Cyanine5.5 anti-mouse CD4 Antibody (BioLegend, catalog #116011, clone RM4-4), PE/Cyanine7 anti-mouse CD8α Antibody (BioLegend ...
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bioRxiv - Immunology 2023Quote: ... Standards for both IL-4 and IL-13 were purchased from BioLegend. IL-10 ELISA kit was purchased from BioLegend
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bioRxiv - Immunology 2023Quote: ... and stained for 30mins at 4°C with: TruStain FcX (Biolegend #422302), CD45-BV421 (clone HI30 ...
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bioRxiv - Immunology 2023Quote: ... and stained for 30mins at 4°C with: TruStain FcX (Biolegend #422302), CD45-AF647 (clone QA17A19 ...
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bioRxiv - Neuroscience 2024Quote: ... The primary incubation was performed overnight at 4°C (phospho-Ser129, BioLegend Cat # 825701 ...
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bioRxiv - Immunology 2024Quote: ... and 10 μg/mL anti-mouse IL-4 antibody (clone 11B11, BioLegend)) or Th2-polarizing condition (10 ng/mL recombinant mouse IL-4 (#574304 ...
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bioRxiv - Immunology 2024Quote: ... CD4 (clone RM4-4) and CD8 (clone 53-6.7) were from Biolegend. Cleaved caspase 3-AF647 (Clone D3E9 ...
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bioRxiv - Neuroscience 2023Quote: ... Sections were then blocked in 5% NDS in TBS for 1 hour before being incubated with 1:1000 6E10 (BioLegend 803004) overnight at 4°C ...