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Citations for BioLegend :
1451 - 1500 of 8552 citations for 5 Pyrimidinecarbonitrile 1 2R 2 3 dihydroxypropyl 1 2 3 4 tetrahydro 3 methyl 2 4 dioxo 9CI since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2022Quote: ... IL-4 PE-Cy7 (BioLegend, cat. 504117, clone 11B11), IL-5 PE (BioLegend ...
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bioRxiv - Cancer Biology 2024Quote: ... and fixed in 1x Fixation Buffer (4% paraformaldehyde, BioLegend). Cells were washed three times with Cell Staining Buffer and stained with Alexa Fluor 647 anti-human Ki-67 antibody (1:100 ...
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bioRxiv - Neuroscience 2023Quote: ... 0.1%TritonX and DAPI (4 ug/mL, #422801, Biolegend) for one hour at room temperature and were stained with vimentin (#677809 ...
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bioRxiv - Immunology 2023Quote: ... CD102-Alexa Fluor 647 (BioLegend; Clone 3C4 (mlC2/4)) ...
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bioRxiv - Immunology 2023Quote: ... CTLA-4-BV421 (clone UC10-4B9; cat. #106311; BioLegend), TIM3-PECF594 (clone B8.2C12 ...
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bioRxiv - Immunology 2023Quote: ... anti-mIL-4 (11B11, BD, 557728, or BioLegend, 504119), anti-m/hIL-5 (TRFK5 ...
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bioRxiv - Plant Biology 2023Quote: ... Then 4 µg of primary antibodies (αHA antibody: Biolegend, #901502 ...
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bioRxiv - Immunology 2023Quote: ... and 500ng/mL anti-IL-4 (500837, BioLegend, USA) purchased from R&D Systems unless stated otherwise ...
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bioRxiv - Immunology 2023Quote: ... anti-IL-4-PE (BioLegend Cat# 504102 (also 504101), RRID:AB_315316) ...
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bioRxiv - Cancer Biology 2023Quote: ... and anti-CD137 antibody (4-1BB; Biolegend Cat #309804) to assess CD8+ T-cell degranulation and TCR activation ...
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bioRxiv - Cancer Biology 2023Quote: ... and anti-CD137 antibody (4-1BB; Biolegend Cat #309804) staining.
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bioRxiv - Immunology 2024Quote: ... and anti-human IL-4 (2μg/ml; Biolegend, Inc).
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bioRxiv - Immunology 2024Quote: ... and anti-mouse IL-4 (10µg/mL; Biolegend, Inc).
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bioRxiv - Microbiology 2023Quote: ... rat anti-mouse CD4 (RM4-5, 1/700, Biolegend, #100548), rat anti-mouse IFN-γ (XMG1.2 ...
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bioRxiv - Immunology 2024Quote: ... CD11b APC/fire (clone CBRM1/5, Biolegend, #301419, 1:150), CD44 BV510 (clone IM7 ...
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bioRxiv - Immunology 2021Quote: ... and stained by intracellular staining for 30 min at 4 °C using a cocktail of the following antibodies: IFN-γ PE (Biolegend, Clone W18272D,1:500), TNF Percp-Cy5.5(Biolegend ...
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bioRxiv - Developmental Biology 2021Quote: ... the cells were pre-incubated for 20 minutes at 4°C in the dark with unlabelled rat anti-mouse CD16/32 (BioLegend – 101320, 1 μg/million cells), then for 1 hour at 4°C in the dark with a 1:1 mix of rat anti-mouse CD41 (labelled with BV421 ...
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bioRxiv - Developmental Biology 2021Quote: ... Single cell suspensions were pre-incubated for 20 minutes at 4°C in the dark with unlabelled rat anti-mouse CD16/32 (BioLegend – 101320, 1 μg/million cells), then stained for one hour at 4°C in the dark with a mix of rat anti-mouse CD117/c-kit (labelled with PE ...
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bioRxiv - Cell Biology 2022Quote: ... and 0.1% Tween-20 and incubated overnight at 4°C in a humidified chamber with primary human anti-CD9 (Biolegend, catalog no. 312102, dilution 1:1000), human anti-CD63 (BD ...
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bioRxiv - Developmental Biology 2024Quote: ... The cells were pelleted and resuspended in FACS buffer for staining with the following antibodies for 20 min at 4° C: Ter119-FITC (BioLegend, Catalog No. 116205, 1/100), EpCAM-PE (BioLegend ...
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bioRxiv - Developmental Biology 2024Quote: ... Samples were then washed with FACS buffer and incubated with the following Lineage antibodies for 30 min at 4°C: CD11b Biotin (BioLegend, Catalog No. 101204, 1/1000), CD11c Biotin (BioLegend ...
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bioRxiv - Immunology 2023Quote: ... and then stained for 20 minutes at 4 °C using the following antibodies: Brilliant Violet 605™ anti-human interferon-gamma (IFN-γ) (506542, Biolegend; 1 μL/well). After being stained ...
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bioRxiv - Cancer Biology 2024Quote: ... approximately 5-10 µg/ml) or purified KPL-1 anti-PSGL-1 mAb (cat. no. 328802, Biolegend). The PL1 hybridoma developed by McEver ...
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bioRxiv - Immunology 2021Quote: ... Extracellular staining was performed in FACS buffer (PBS + 2% FBS + 2mM EDTA) with the following antibodies: CD4 (GK1.5, Biolegend), CD8 (53-6.7 ...
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bioRxiv - Immunology 2021Quote: ... were co-incubated with 6×105 target cells expressing mCherry at 37°C for 2 hours in the presence of 2µM monensin (BioLegend). The cells were centrifuged ...
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bioRxiv - Immunology 2022Quote: Serum samples were screened for neutralization using LEGENDplex SARS-CoV-2 neutralizing antibody assay (BioLegend, San Diego, CA, USA) following manufacturer recommendations ...
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bioRxiv - Cancer Biology 2021Quote: ... then resuspended in FACS buffer containing 2 ug/ml APC/Cy7 anti-human HLA-A,B,C (BioLegend, #311425). Cells were incubated with the antibody on ice for 20 minutes in the dark ...
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bioRxiv - Cancer Biology 2022Quote: ... Cells were blocked in FSC buffer (2% FBS/1x PBS) containing 0.5 µg anti-CD16/32 antibody (TruStain FcX Plus, BioLegend) for 10 min at 4°C to block non-specific binding of antibodies via Fc receptors ...
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bioRxiv - Immunology 2022Quote: ... SARS-CoV-2 biotinylated RBD protein (His & AVI Tag) (SinoBiological, 40592-V27H-B) conjugated with APC-streptavidin (BioLegend, 405207), and 7-AAD (Invitrogen ...
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bioRxiv - Neuroscience 2022Quote: ... Muscle sections were incubated overnight at 4 °C in blocking solution (2 % BSA, 10 % Normal Goat Serum, 0.1 % Triton and PBS) with chicken anti-NFM (#822701, BioLegend, USA ...
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bioRxiv - Cancer Biology 2022Quote: ... cells were pre-incubated for 2 h with the Cell Activation Cocktail containing Brefeldin A according to the manufacturer’s instructions (BioLegend). After surface staining ...
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bioRxiv - Microbiology 2020Quote: ... TNF-α and IL-2 levels were quantified in co-culture supernatants by ELISA (BioLegend, San Diego, CA, USA).
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bioRxiv - Cancer Biology 2022Quote: ... Cells were then blocked in FSC buffer (2% FBS/1x PBS) containing 0.5 μg anti-CD16/32 antibody (TruStain FcX Plus, BioLegend) for 10 min at 4°C to block non-specific binding of antibodies via Fc receptors ...
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bioRxiv - Immunology 2019Quote: ... 2 ml medium/well) were cultured in growth media supplemented with GM-CSF (100 ng/ml, BioLegend, San Diego), TGF-β (10 ng/ml ...
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bioRxiv - Bioengineering 2020Quote: ... recombinant AAV9 (2×109 vg/well) and SpCas9 protein (0.27 μg/well) were diluted in 1x Coating Buffer A (BioLegend) and used to coat a 96-well Nunc MaxiSorp plate ...
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bioRxiv - Genomics 2021Quote: ... Isolated 2×106 PBMC cells were incubated with PMA/ionomycin (i.e., stimulated) (Cell Activation Cocktail with Brefeldin A, Biolegend), or DMSO as control (i.e. ...
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bioRxiv - Genomics 2021Quote: ... B cells were incubated with viruses for 2 hr and then stained with Human TruStain FcXTM (Fc Block, BioLegend), CD19-APC (clone HIB19 ...
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bioRxiv - Cancer Biology 2020Quote: ... a maximum of 2 million cells per sample was resuspended in 100 µl of cell staining buffer (Biolegend, USA) with 5 µl of Fc receptor Block (TrueStain FcX ...
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bioRxiv - Cancer Biology 2022Quote: ... washed in blocking solution (2% BSA in PBS) and immunolabeled with anti-CD24 PerCP/Cy5.5 and anti-CD44 FITC primary antibodies (BioLegend, 1:50 ...
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bioRxiv - Immunology 2022Quote: ... for 30mins at 37°C, washed, resuspended in FACS buffer (PBS, 2% FBS) and co-stained with anti-CD4 (GK1.5, Biolegend), anti-CD62L (MEL-14 ...
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bioRxiv - Immunology 2022Quote: ... and nonspecific staining was then blocked by addition of anti-CD16/32 (2 μg/ml; mouse TrueStain FcX, BioLegend), 2% rat serum (Invitrogen) ...
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bioRxiv - Immunology 2022Quote: ... CD19+ B cells were stimulated for 24 hours using Purified F(ab’)2 Goat anti-mouse IgM Antibody (BioLegend). CD14+ monocytes were stimulated for 24 hours with 1ug/ml of LPS ...
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bioRxiv - Microbiology 2022Quote: ... then retro-orbitally injected with 2 μg/mouse of APC anti-mouse CD45 (Biolegend, cat#103112; clone 30-F11) antibody in 50 μL PBS for intravenous labeling of CD45+ circulating lymphocytes 15 minutes prior to euthanization ...
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bioRxiv - Immunology 2022Quote: ... SARS-CoV-2 biotinylated RBD protein (His & AVI Tag) (SinoBiological, 40592-V27H-B) conjugated to APC-streptavidin (BioLegend, 405207). After washed twice ...
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bioRxiv - Cell Biology 2022Quote: ... Cells were then washed with suspension buffer (PBS + 0.5% BSA + 2 mM EDTA + 4.5 mg/mL D-glucose) and resuspended in RBC Lysis Buffer (Biolegend 420301) for one minute at room temperature to remove red blood cells ...
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bioRxiv - Microbiology 2023Quote: ... MBL) or H-2q-restricted LCMV NP118 peptide (MBL) and 50 U/mL of recombinant mouse IL-2 (BioLegend) for 1 h ...
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bioRxiv - Cell Biology 2023Quote: ... approximately 200,000 cells per sample were resuspended in staining buffer (PBS, 2% BSA, and 0.01% Tween) and incubated for 10 minutes with Fc block (TruStain FcX (BioLegend) and FcR blocking reagent (Miltenyi) ...
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bioRxiv - Immunology 2023Quote: ... and resuspended in FACS buffer (PBS with 0.5% BSA and 2 mM EDTA) with Fc blocked using human TrueStain FcX (Biolegend) and incubated in the dark for 10 min at room temperature ...
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bioRxiv - Bioengineering 2023Quote: ... cells were washed with FACS buffer (PBS + 2% FBS + 0.05% NaN3) and incubated with Fc receptor blocking solution (Human TruStain FcX, BioLegend) for 5 minutes ...
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bioRxiv - Immunology 2023Quote: ... Cells were subsequently resuspended in staining buffer (PBS with 0.5% bovine serum albumin and 2 mM EDTA) and Fc-blocked using TruStain FcX reagent (BioLegend). Cells were then stained with the following fluorophore-conjugated anti-mouse antibodies for 30 min at 4°C ...