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Citations for BioLegend :
1401 - 1450 of 7971 citations for 5 Pyrimidinecarbonitrile 1 2R 2 3 dihydroxypropyl 1 2 3 4 tetrahydro 3 methyl 2 4 dioxo 9CI since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2020Quote: ... recombinant AAV9 (2×109 vg/well) and SpCas9 protein (0.27 μg/well) were diluted in 1x Coating Buffer A (BioLegend) and used to coat a 96-well Nunc MaxiSorp plate ...
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bioRxiv - Genomics 2021Quote: ... Isolated 2×106 PBMC cells were incubated with PMA/ionomycin (i.e., stimulated) (Cell Activation Cocktail with Brefeldin A, Biolegend), or DMSO as control (i.e. ...
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bioRxiv - Genomics 2021Quote: ... B cells were incubated with viruses for 2 hr and then stained with Human TruStain FcXTM (Fc Block, BioLegend), CD19-APC (clone HIB19 ...
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bioRxiv - Cancer Biology 2020Quote: ... a maximum of 2 million cells per sample was resuspended in 100 µl of cell staining buffer (Biolegend, USA) with 5 µl of Fc receptor Block (TrueStain FcX ...
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bioRxiv - Cancer Biology 2022Quote: ... washed in blocking solution (2% BSA in PBS) and immunolabeled with anti-CD24 PerCP/Cy5.5 and anti-CD44 FITC primary antibodies (BioLegend, 1:50 ...
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bioRxiv - Immunology 2022Quote: ... for 30mins at 37°C, washed, resuspended in FACS buffer (PBS, 2% FBS) and co-stained with anti-CD4 (GK1.5, Biolegend), anti-CD62L (MEL-14 ...
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bioRxiv - Immunology 2022Quote: ... and nonspecific staining was then blocked by addition of anti-CD16/32 (2 μg/ml; mouse TrueStain FcX, BioLegend), 2% rat serum (Invitrogen) ...
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bioRxiv - Immunology 2022Quote: ... CD19+ B cells were stimulated for 24 hours using Purified F(ab’)2 Goat anti-mouse IgM Antibody (BioLegend). CD14+ monocytes were stimulated for 24 hours with 1ug/ml of LPS ...
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bioRxiv - Microbiology 2022Quote: ... then retro-orbitally injected with 2 μg/mouse of APC anti-mouse CD45 (Biolegend, cat#103112; clone 30-F11) antibody in 50 μL PBS for intravenous labeling of CD45+ circulating lymphocytes 15 minutes prior to euthanization ...
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bioRxiv - Immunology 2022Quote: ... SARS-CoV-2 biotinylated RBD protein (His & AVI Tag) (SinoBiological, 40592-V27H-B) conjugated to APC-streptavidin (BioLegend, 405207). After washed twice ...
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bioRxiv - Cell Biology 2022Quote: ... Cells were then washed with suspension buffer (PBS + 0.5% BSA + 2 mM EDTA + 4.5 mg/mL D-glucose) and resuspended in RBC Lysis Buffer (Biolegend 420301) for one minute at room temperature to remove red blood cells ...
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bioRxiv - Cell Biology 2023Quote: ... approximately 200,000 cells per sample were resuspended in staining buffer (PBS, 2% BSA, and 0.01% Tween) and incubated for 10 minutes with Fc block (TruStain FcX (BioLegend) and FcR blocking reagent (Miltenyi) ...
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bioRxiv - Microbiology 2023Quote: ... MBL) or H-2q-restricted LCMV NP118 peptide (MBL) and 50 U/mL of recombinant mouse IL-2 (BioLegend) for 1 h ...
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bioRxiv - Immunology 2023Quote: ... and resuspended in FACS buffer (PBS with 0.5% BSA and 2 mM EDTA) with Fc blocked using human TrueStain FcX (Biolegend) and incubated in the dark for 10 min at room temperature ...
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bioRxiv - Bioengineering 2023Quote: ... cells were washed with FACS buffer (PBS + 2% FBS + 0.05% NaN3) and incubated with Fc receptor blocking solution (Human TruStain FcX, BioLegend) for 5 minutes ...
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bioRxiv - Immunology 2023Quote: ... Cells were subsequently resuspended in staining buffer (PBS with 0.5% bovine serum albumin and 2 mM EDTA) and Fc-blocked using TruStain FcX reagent (BioLegend). Cells were then stained with the following fluorophore-conjugated anti-mouse antibodies for 30 min at 4°C ...
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bioRxiv - Immunology 2023Quote: ... cells were resuspended in FACS buffer (PBS with 0.5% BSA and 2 mM EDTA) and Fc blocked using human TrueStain FcX (Biolegend). For cell surface staining ...
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bioRxiv - Immunology 2023Quote: Surface staining for flow cytometry analysis was performed in PBS supplemented with 2% FBS and Trustain FcX (Biolegend, 422301) at room temperature ...
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bioRxiv - Immunology 2023Quote: ... Enriched CellTrace Violet-labeled OT-I CD8+ T cells were resuspended at 20,000 cells/100uL in complete RPMI + 10ng/mL of recombinant mouse IL-2 (Biolegend). As positive control ...
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bioRxiv - Immunology 2021Quote: ... Nonspecific binding was blocked by incubation for 10 min at 4 °C with anti-CD16/CD32 antibody (Biolegend, clone 93, 5 ng/ μL) antibody and stained with the appropriate antibodies for 15 min at 4 °C ...
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bioRxiv - Pathology 2019Quote: ... IL-9 and IL-6 determinations (Human IL-4, IL-5, IL-9 and IL-6 MAX, Biolegend, San Diego, CA 92121 USA) according to manufacturer’s protocol ...
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bioRxiv - Immunology 2020Quote: ... IL-4-APC (clone MP4-25D2, BioLegend Cat# 500812, RRID:AB_315131), IL-17A-AlexaFluor488 (clone eBio64Dec17 ...
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bioRxiv - Neuroscience 2019Quote: ... and in 4 μl of APC anti-HA antibody (Biolegend). Bound GPC6 antibody were detected by incubating with a 1:200 dilution of goat anti-mouse Alexa-488 secondary antibody in 2% BSA for 45 min on ice ...
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bioRxiv - Immunology 2019Quote: ... 4 were analyzed with the following cocktail: CD4-BV650 (Biolegend), CD25-BV605(Biolegend) ...
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bioRxiv - Cancer Biology 2021Quote: ... cells were incubated for 4 hours with Brefeldin A (Biolegend) and Monensin (Sigma Aldrich) ...
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bioRxiv - Microbiology 2022Quote: ... 4°C) and stained with CD11b-APC (M1/70, Biolegend), CD19-PE (HIB19 ...
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bioRxiv - Immunology 2022Quote: ... and anti-CD62L PeCy7 (Cat#104418, Clone# MEL/4, BioLegend) on ice for 30 minutes (Supplemental Table 6) ...
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bioRxiv - Immunology 2020Quote: ... Tbet BV605 (clone 4B10, BioLegend, cat# 644817, dilution 4:100); Bcl6 PE/Dazzle (clone 7D1 ...
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bioRxiv - Immunology 2020Quote: ... ELISA MAX capture anti-IL-4 (previous antibodies from Biolegend) or capture TNFα (R&D systems ...
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bioRxiv - Genomics 2022Quote: ... 50 ng/mL of recombinant IL-4 (BioLegend, Cat# 574302) and 1 μg/mL of soluble anti-CD28 for 6 days at 37°C ...
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bioRxiv - Immunology 2022Quote: ... and stained overnight at 4°C with avidin-FITC (BioLegend), anti-human/mouse CD117 (c-kit ...
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bioRxiv - Immunology 2022Quote: ... at 4°C for 15 minutes with antibody 3D12 (BioLegend) at 5 ng/μl ...
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bioRxiv - Microbiology 2022Quote: ... CD137 (4-1BB) PE (2.5 μg/mL, BioLegend Cat. 106106), and CD197 (CCR7 ...
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bioRxiv - Cell Biology 2023Quote: ... and 4 uL APC conjugated anti-CD9 (clone H19a, Biolegend). Excess antibody was removed by two washes with 4°C PBS ...
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bioRxiv - Microbiology 2023Quote: ... 4°C) and stained with CD11b-APC (M1/70, Biolegend), CD19-PE (HIB19 ...
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bioRxiv - Immunology 2023Quote: ... 10 μg/mL anti-IL-4 mAb (11B11, BioLegend, 504122); Th2 condition ...
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bioRxiv - Bioengineering 2023Quote: ... Anti-SSEA-4 PE (2.5 µl/test, cat. 330406, Biolegend) and Anti-TRA-1-60 Alexa Fluor 647 (2.5 µl/test ...
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bioRxiv - Genomics 2023Quote: ... TotalSeq™-B0254 anti-human Hashtag 4 Antibody (394637, Biolegend) and TotalSeq™-B0255 anti-human Hashtag 5 Antibody (394639 ...
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bioRxiv - Immunology 2023Quote: ... and IL-4 (20 ng/ml, Biolegend, San Diego, USA). Cells were allowed to differentiate for 5-7 days (DC-SIGN+ cells in the culture > 90%).
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bioRxiv - Immunology 2023Quote: ... and 10 ng/mL of recombinant mouse IL-4 (BioLegend) diluted in PBS was added to wells cultured with the cytokine ...
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bioRxiv - Immunology 2023Quote: ... and anti-mouse TotalSeq-A Hashtag antibody (4-6; BioLegend). The enriched samples were then stained with APC-labeled Streptavidin (BioLegend ...
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bioRxiv - Immunology 2024Quote: ... Brilliant Violet 605 anti CTLA-4 clone UC10-4B9 (Biolegend), FITC anti-Foxp3 clone FJK-16s (eBioscience) ...
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bioRxiv - Cancer Biology 2024Quote: ... and then fixed in 1x Fixation Buffer (4% paraformaldehyde, BioLegend). Cells were washed 3 times with Cell Staining Buffer and stained with either Alexa Fluor (AF ...
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bioRxiv - Immunology 2024Quote: ... and stained with anti-IFNγ–PE (clone 4 S.B3, BioLegend) for 30 minutes at room temperature ...
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bioRxiv - Immunology 2024Quote: ... 10 ng/ml of recombinant IL-4 (Cat# 574302, BioLegend), and 10 ng/ml of recombinant IL-13 (Cat# 575902 ...
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bioRxiv - Pathology 2022Quote: ... and cells were stained in FACS buffer for 30 min at 4°C with the following anti-mouse antibodies: PacificBlue-conjugated anti-CD45 (Biolegend, clone 30-F11, #103126, 1/100), PE-Cy7-conjugated anti-CD11b (BD Biosciences ...
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bioRxiv - Cancer Biology 2020Quote: ... and polyclonal chicken anti-Keratin 5 (Biolegend, Cat: 905901, 1:100). Subsequently ...
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bioRxiv - Immunology 2020Quote: ... CD4-Alexa Fluor 488 (clone: RM4-5; dilution 1:400, BioLegend), CD8a-APC/Cy7 (clone ...
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bioRxiv - Immunology 2021Quote: ... CD4 (PerCP/BV605, Tonbo Bioscience/Biolegend, 1:200, clone RM4-5), CD8a (APC-Cy7/APC-H7/APC ...
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bioRxiv - Cancer Biology 2022Quote: ... at 1:5 dilution and anti-human CD45-APC (BioLegend 304012) at 1:10 dilution ...